Hair Regeneration Potential of Human Dermal Sheath Cells Cultured Under Physiological Oxygen
We investigated the effect of oxygen tension on the proliferation and hair-inductive capacity of human dermal papilla cells (DPCs) and dermal sheath cells (DSCs). DPCs and DSCs were separately obtained from human hair follicles and each cultured under atmospheric/hyperoxic (20% O 2 ), physiological/...
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| Veröffentlicht in: | Tissue engineering. Part A 2020-11, Vol.26 (21-22), p.1147-1157 |
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| creator | Kanayama, Koji Takada, Hitomi Saito, Natsumi Kato, Harunosuke Kinoshita, Kahori Shirado, Takako Mashiko, Takanobu Asahi, Rintaro Mori, Masanori Tashiro, Kensuke Sunaga, Ataru Kurisaki, Akira Yoshizato, Katsutoshi Yoshimura, Kotaro |
| description | We investigated the effect of oxygen tension on the proliferation and hair-inductive capacity of human dermal papilla cells (DPCs) and dermal sheath cells (DSCs). DPCs and DSCs were separately obtained from human hair follicles and each cultured under atmospheric/hyperoxic (20% O
2
), physiological/normoxic (6% O
2
), or hypoxic (1% O
2
) conditions. Proliferation of DPCs and DSCs was highest under normoxia. Compared with hyperoxia, hypoxia inhibited proliferation of DPCs, but enhanced that of DSCs. In DPCs, hypoxia downregulated the expression of hair-inductive capacity-related genes, including
BMP4
,
LEF1
,
SOX2
, and
VCAN
. In DSCs, both normoxia and hypoxia upregulated
SOX2
expression, whereas hypoxia downregulated
BMP4
expression. Microarray analysis revealed that normoxia increased the expression of pluripotency-related genes, including
SPRY
,
NR0B1
,
MSX2
,
IFITM1
, and
DAZL
, compared with hyperoxia. In an
in vivo
hair follicle reconstitution assay, cultured DPCs and DSCs were transplanted with newborn mouse epidermal keratinocytes into nude mice using a chamber method. In this experiment, normoxia resulted in the most efficient induction of DPC hair follicles, whereas hypoxia caused the most efficient induction and maturation of DSC hair follicles. These results suggest that application of physiological/hypoxic oxygen tension to cultured human DSCs enhances proliferation and maintenance of hair inductivity for skin engineering and clinical applications. |
| doi_str_mv | 10.1089/ten.tea.2019.0329 |
| format | Article |
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2
), physiological/normoxic (6% O
2
), or hypoxic (1% O
2
) conditions. Proliferation of DPCs and DSCs was highest under normoxia. Compared with hyperoxia, hypoxia inhibited proliferation of DPCs, but enhanced that of DSCs. In DPCs, hypoxia downregulated the expression of hair-inductive capacity-related genes, including
BMP4
,
LEF1
,
SOX2
, and
VCAN
. In DSCs, both normoxia and hypoxia upregulated
SOX2
expression, whereas hypoxia downregulated
BMP4
expression. Microarray analysis revealed that normoxia increased the expression of pluripotency-related genes, including
SPRY
,
NR0B1
,
MSX2
,
IFITM1
, and
DAZL
, compared with hyperoxia. In an
in vivo
hair follicle reconstitution assay, cultured DPCs and DSCs were transplanted with newborn mouse epidermal keratinocytes into nude mice using a chamber method. In this experiment, normoxia resulted in the most efficient induction of DPC hair follicles, whereas hypoxia caused the most efficient induction and maturation of DSC hair follicles. These results suggest that application of physiological/hypoxic oxygen tension to cultured human DSCs enhances proliferation and maintenance of hair inductivity for skin engineering and clinical applications.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2019.0329</identifier><identifier>PMID: 32408803</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Cell culture ; Cell proliferation ; Follicles ; Hyperoxia ; Hypoxia ; Keratinocytes ; Msx2 protein ; Original Articles ; Oxygen tension ; Physiology ; Pluripotency ; Skin ; Therapeutic applications ; Wound healing</subject><ispartof>Tissue engineering. Part A, 2020-11, Vol.26 (21-22), p.1147-1157</ispartof><rights>2020, Mary Ann Liebert, Inc., publishers</rights><rights>Copyright Mary Ann Liebert, Inc. Nov 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-7788ac05f12827961233deade3d9b4cfcc1fbd9a89a0dc41f076d065d11160563</citedby><cites>FETCH-LOGICAL-c491t-7788ac05f12827961233deade3d9b4cfcc1fbd9a89a0dc41f076d065d11160563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32408803$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kanayama, Koji</creatorcontrib><creatorcontrib>Takada, Hitomi</creatorcontrib><creatorcontrib>Saito, Natsumi</creatorcontrib><creatorcontrib>Kato, Harunosuke</creatorcontrib><creatorcontrib>Kinoshita, Kahori</creatorcontrib><creatorcontrib>Shirado, Takako</creatorcontrib><creatorcontrib>Mashiko, Takanobu</creatorcontrib><creatorcontrib>Asahi, Rintaro</creatorcontrib><creatorcontrib>Mori, Masanori</creatorcontrib><creatorcontrib>Tashiro, Kensuke</creatorcontrib><creatorcontrib>Sunaga, Ataru</creatorcontrib><creatorcontrib>Kurisaki, Akira</creatorcontrib><creatorcontrib>Yoshizato, Katsutoshi</creatorcontrib><creatorcontrib>Yoshimura, Kotaro</creatorcontrib><title>Hair Regeneration Potential of Human Dermal Sheath Cells Cultured Under Physiological Oxygen</title><title>Tissue engineering. Part A</title><addtitle>Tissue Eng Part A</addtitle><description>We investigated the effect of oxygen tension on the proliferation and hair-inductive capacity of human dermal papilla cells (DPCs) and dermal sheath cells (DSCs). DPCs and DSCs were separately obtained from human hair follicles and each cultured under atmospheric/hyperoxic (20% O
2
), physiological/normoxic (6% O
2
), or hypoxic (1% O
2
) conditions. Proliferation of DPCs and DSCs was highest under normoxia. Compared with hyperoxia, hypoxia inhibited proliferation of DPCs, but enhanced that of DSCs. In DPCs, hypoxia downregulated the expression of hair-inductive capacity-related genes, including
BMP4
,
LEF1
,
SOX2
, and
VCAN
. In DSCs, both normoxia and hypoxia upregulated
SOX2
expression, whereas hypoxia downregulated
BMP4
expression. Microarray analysis revealed that normoxia increased the expression of pluripotency-related genes, including
SPRY
,
NR0B1
,
MSX2
,
IFITM1
, and
DAZL
, compared with hyperoxia. In an
in vivo
hair follicle reconstitution assay, cultured DPCs and DSCs were transplanted with newborn mouse epidermal keratinocytes into nude mice using a chamber method. In this experiment, normoxia resulted in the most efficient induction of DPC hair follicles, whereas hypoxia caused the most efficient induction and maturation of DSC hair follicles. These results suggest that application of physiological/hypoxic oxygen tension to cultured human DSCs enhances proliferation and maintenance of hair inductivity for skin engineering and clinical applications.</description><subject>Cell culture</subject><subject>Cell proliferation</subject><subject>Follicles</subject><subject>Hyperoxia</subject><subject>Hypoxia</subject><subject>Keratinocytes</subject><subject>Msx2 protein</subject><subject>Original Articles</subject><subject>Oxygen tension</subject><subject>Physiology</subject><subject>Pluripotency</subject><subject>Skin</subject><subject>Therapeutic applications</subject><subject>Wound healing</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqNkF1LwzAUhoMofkx_gDcS8Mab1aTp0uZS5seEgUMdeCGUNDl1lTbRJAX3703Z9MIrCSEn4TkvJw9Cp5QklBTiMoBJAsgkJVQkhKViBx1SwfIxY5OX3d86owfoyPt3Qjjheb6PDliakaIg7BC9zmTj8CO8gQEnQ2MNXtiYGxrZYlvjWd9Jg6_BdfH-tAIZVngKbevxtG9D70DjpdHg8GK19o1t7VujIvnwtY6Jx2ivlq2Hk-05Qsvbm-fpbDx_uLufXs3HKhM0jPO8KKQik5qmRZoLTlPGNEgNTIsqU7VStK60kIWQRKuM1iTnmvCJppRyMuFshC42uR_OfvbgQ9k1XsUppQHb-zL-dlicZRE9_4O-296ZOF2kOCVZwZiIFN1QylnvHdTlh2s66dYlJeWgvoyK4pbloL4c1Mees21yX3Wgfzt-XEcg3wDDszSmbaACF_4R_Q17NJMH</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Kanayama, Koji</creator><creator>Takada, Hitomi</creator><creator>Saito, Natsumi</creator><creator>Kato, Harunosuke</creator><creator>Kinoshita, Kahori</creator><creator>Shirado, Takako</creator><creator>Mashiko, Takanobu</creator><creator>Asahi, Rintaro</creator><creator>Mori, Masanori</creator><creator>Tashiro, Kensuke</creator><creator>Sunaga, Ataru</creator><creator>Kurisaki, Akira</creator><creator>Yoshizato, Katsutoshi</creator><creator>Yoshimura, Kotaro</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20201101</creationdate><title>Hair Regeneration Potential of Human Dermal Sheath Cells Cultured Under Physiological Oxygen</title><author>Kanayama, Koji ; Takada, Hitomi ; Saito, Natsumi ; Kato, Harunosuke ; Kinoshita, Kahori ; Shirado, Takako ; Mashiko, Takanobu ; Asahi, Rintaro ; Mori, Masanori ; Tashiro, Kensuke ; Sunaga, Ataru ; Kurisaki, Akira ; Yoshizato, Katsutoshi ; Yoshimura, Kotaro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-7788ac05f12827961233deade3d9b4cfcc1fbd9a89a0dc41f076d065d11160563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Cell culture</topic><topic>Cell proliferation</topic><topic>Follicles</topic><topic>Hyperoxia</topic><topic>Hypoxia</topic><topic>Keratinocytes</topic><topic>Msx2 protein</topic><topic>Original Articles</topic><topic>Oxygen tension</topic><topic>Physiology</topic><topic>Pluripotency</topic><topic>Skin</topic><topic>Therapeutic applications</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kanayama, Koji</creatorcontrib><creatorcontrib>Takada, Hitomi</creatorcontrib><creatorcontrib>Saito, Natsumi</creatorcontrib><creatorcontrib>Kato, Harunosuke</creatorcontrib><creatorcontrib>Kinoshita, Kahori</creatorcontrib><creatorcontrib>Shirado, Takako</creatorcontrib><creatorcontrib>Mashiko, Takanobu</creatorcontrib><creatorcontrib>Asahi, Rintaro</creatorcontrib><creatorcontrib>Mori, Masanori</creatorcontrib><creatorcontrib>Tashiro, Kensuke</creatorcontrib><creatorcontrib>Sunaga, Ataru</creatorcontrib><creatorcontrib>Kurisaki, Akira</creatorcontrib><creatorcontrib>Yoshizato, Katsutoshi</creatorcontrib><creatorcontrib>Yoshimura, Kotaro</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Tissue engineering. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kanayama, Koji</au><au>Takada, Hitomi</au><au>Saito, Natsumi</au><au>Kato, Harunosuke</au><au>Kinoshita, Kahori</au><au>Shirado, Takako</au><au>Mashiko, Takanobu</au><au>Asahi, Rintaro</au><au>Mori, Masanori</au><au>Tashiro, Kensuke</au><au>Sunaga, Ataru</au><au>Kurisaki, Akira</au><au>Yoshizato, Katsutoshi</au><au>Yoshimura, Kotaro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hair Regeneration Potential of Human Dermal Sheath Cells Cultured Under Physiological Oxygen</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2020-11-01</date><risdate>2020</risdate><volume>26</volume><issue>21-22</issue><spage>1147</spage><epage>1157</epage><pages>1147-1157</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>We investigated the effect of oxygen tension on the proliferation and hair-inductive capacity of human dermal papilla cells (DPCs) and dermal sheath cells (DSCs). DPCs and DSCs were separately obtained from human hair follicles and each cultured under atmospheric/hyperoxic (20% O
2
), physiological/normoxic (6% O
2
), or hypoxic (1% O
2
) conditions. Proliferation of DPCs and DSCs was highest under normoxia. Compared with hyperoxia, hypoxia inhibited proliferation of DPCs, but enhanced that of DSCs. In DPCs, hypoxia downregulated the expression of hair-inductive capacity-related genes, including
BMP4
,
LEF1
,
SOX2
, and
VCAN
. In DSCs, both normoxia and hypoxia upregulated
SOX2
expression, whereas hypoxia downregulated
BMP4
expression. Microarray analysis revealed that normoxia increased the expression of pluripotency-related genes, including
SPRY
,
NR0B1
,
MSX2
,
IFITM1
, and
DAZL
, compared with hyperoxia. In an
in vivo
hair follicle reconstitution assay, cultured DPCs and DSCs were transplanted with newborn mouse epidermal keratinocytes into nude mice using a chamber method. In this experiment, normoxia resulted in the most efficient induction of DPC hair follicles, whereas hypoxia caused the most efficient induction and maturation of DSC hair follicles. These results suggest that application of physiological/hypoxic oxygen tension to cultured human DSCs enhances proliferation and maintenance of hair inductivity for skin engineering and clinical applications.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>32408803</pmid><doi>10.1089/ten.tea.2019.0329</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1937-3341 |
| ispartof | Tissue engineering. Part A, 2020-11, Vol.26 (21-22), p.1147-1157 |
| issn | 1937-3341 1937-335X |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Cell culture Cell proliferation Follicles Hyperoxia Hypoxia Keratinocytes Msx2 protein Original Articles Oxygen tension Physiology Pluripotency Skin Therapeutic applications Wound healing |
| title | Hair Regeneration Potential of Human Dermal Sheath Cells Cultured Under Physiological Oxygen |
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