Pathogenesis and transmission of SARS-CoV-2 in golden hamsters
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus with high nucleotide identity to SARS-CoV and to SARS-related coronaviruses that have been detected in horseshoe bats, has spread across the world and had a global effect on healthcare systems and economies 1 , 2 . A s...
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| Veröffentlicht in: | Nature (London) 2020-07, Vol.583 (7818), p.834-838 |
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| creator | Sia, Sin Fun Yan, Li-Meng Chin, Alex W. H. Fung, Kevin Choy, Ka-Tim Wong, Alvina Y. L. Kaewpreedee, Prathanporn Perera, Ranawaka A. P. M. Poon, Leo L. M. Nicholls, John M. Peiris, Malik Yen, Hui-Ling |
| description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus with high nucleotide identity to SARS-CoV and to SARS-related coronaviruses that have been detected in horseshoe bats, has spread across the world and had a global effect on healthcare systems and economies
1
,
2
. A suitable small animal model is needed to support the development of vaccines and therapies. Here we report the pathogenesis and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters (
Mesocricetus auratus
). Immunohistochemistry assay demonstrated the presence of viral antigens in nasal mucosa, bronchial epithelial cells and areas of lung consolidation on days 2 and 5 after inoculation with SARS-CoV-2, followed by rapid viral clearance and pneumocyte hyperplasia at 7 days after inoculation. We also found viral antigens in epithelial cells of the duodenum, and detected viral RNA in faeces. Notably, SARS-CoV-2 was transmitted efficiently from inoculated hamsters to naive hamsters by direct contact and via aerosols. Transmission via fomites in soiled cages was not as efficient. Although viral RNA was continuously detected in the nasal washes of inoculated hamsters for 14 days, the communicable period was short and correlated with the detection of infectious virus but not viral RNA. Inoculated and naturally infected hamsters showed apparent weight loss on days 6–7 post-inoculation or post-contact; all hamsters returned to their original weight within 14 days and developed neutralizing antibodies. Our results suggest that features associated with SARS-CoV-2 infection in golden hamsters resemble those found in humans with mild SARS-CoV-2 infections.
The pathogenicity and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters resemble features of COVID-19 in human patients, suggesting that these hamsters could be used to model this disease. |
| doi_str_mv | 10.1038/s41586-020-2342-5 |
| format | Article |
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1
,
2
. A suitable small animal model is needed to support the development of vaccines and therapies. Here we report the pathogenesis and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters (
Mesocricetus auratus
). Immunohistochemistry assay demonstrated the presence of viral antigens in nasal mucosa, bronchial epithelial cells and areas of lung consolidation on days 2 and 5 after inoculation with SARS-CoV-2, followed by rapid viral clearance and pneumocyte hyperplasia at 7 days after inoculation. We also found viral antigens in epithelial cells of the duodenum, and detected viral RNA in faeces. Notably, SARS-CoV-2 was transmitted efficiently from inoculated hamsters to naive hamsters by direct contact and via aerosols. Transmission via fomites in soiled cages was not as efficient. Although viral RNA was continuously detected in the nasal washes of inoculated hamsters for 14 days, the communicable period was short and correlated with the detection of infectious virus but not viral RNA. Inoculated and naturally infected hamsters showed apparent weight loss on days 6–7 post-inoculation or post-contact; all hamsters returned to their original weight within 14 days and developed neutralizing antibodies. Our results suggest that features associated with SARS-CoV-2 infection in golden hamsters resemble those found in humans with mild SARS-CoV-2 infections.
The pathogenicity and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters resemble features of COVID-19 in human patients, suggesting that these hamsters could be used to model this disease.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/s41586-020-2342-5</identifier><identifier>PMID: 32408338</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/326/596/2555 ; 631/326/596/2563 ; 631/326/596/4130 ; 64 ; Aerosols ; Alveolar Epithelial Cells - pathology ; Alveolar Epithelial Cells - virology ; Animal models ; Animals ; Antibodies ; Antibodies, Neutralizing - immunology ; Antibodies, Viral - immunology ; Antigens ; Antigens, Viral - immunology ; Antigens, Viral - isolation & purification ; Antigens, Viral - metabolism ; Betacoronavirus - immunology ; Betacoronavirus - isolation & purification ; Betacoronavirus - metabolism ; Betacoronavirus - pathogenicity ; Bronchi - pathology ; Bronchi - virology ; Coronaviridae ; Coronavirus Infections - immunology ; Coronavirus Infections - transmission ; Coronavirus Infections - virology ; Coronaviruses ; COVID-19 ; Disease Models, Animal ; Disease transmission ; Duodenum ; Duodenum - virology ; Epithelial cells ; Fomites ; Fomites - virology ; Hamsters ; Housing, Animal ; Humanities and Social Sciences ; Hyperplasia ; Immunization ; Immunohistochemistry ; Infections ; Influenza ; Inoculation ; Kidney - virology ; Lung - pathology ; Lung - virology ; Lungs ; Male ; Mesocricetus - immunology ; Mesocricetus - virology ; Mesocricetus auratus ; Mucosa ; multidisciplinary ; Nasal Mucosa - virology ; Neurons ; Nucleotides ; Pandemics ; Pathogenesis ; Pneumonia ; Pneumonia, Viral - immunology ; Pneumonia, Viral - transmission ; Pneumonia, Viral - virology ; Proteins ; Ribonucleic acid ; RNA ; RNA viruses ; RNA, Viral - analysis ; SARS-CoV-2 ; Science ; Science (multidisciplinary) ; Severe acute respiratory syndrome coronavirus 2 ; Transgenic animals ; Vaccines ; Viral diseases ; Viral Load ; Viruses ; Weight Loss</subject><ispartof>Nature (London), 2020-07, Vol.583 (7818), p.834-838</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Limited 2020</rights><rights>Copyright Nature Publishing Group Jul 30, 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-ea9cf4cc3b8a5c54a34bddcb4194664037dd1ce8dd6fe0586a2f321b06bce8563</citedby><cites>FETCH-LOGICAL-c518t-ea9cf4cc3b8a5c54a34bddcb4194664037dd1ce8dd6fe0586a2f321b06bce8563</cites><orcidid>0000-0001-8217-5995 ; 0000-0003-2493-3609 ; 0000-0002-0464-2401 ; 0000-0003-3936-1535</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32408338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sia, Sin Fun</creatorcontrib><creatorcontrib>Yan, Li-Meng</creatorcontrib><creatorcontrib>Chin, Alex W. H.</creatorcontrib><creatorcontrib>Fung, Kevin</creatorcontrib><creatorcontrib>Choy, Ka-Tim</creatorcontrib><creatorcontrib>Wong, Alvina Y. L.</creatorcontrib><creatorcontrib>Kaewpreedee, Prathanporn</creatorcontrib><creatorcontrib>Perera, Ranawaka A. P. M.</creatorcontrib><creatorcontrib>Poon, Leo L. M.</creatorcontrib><creatorcontrib>Nicholls, John M.</creatorcontrib><creatorcontrib>Peiris, Malik</creatorcontrib><creatorcontrib>Yen, Hui-Ling</creatorcontrib><title>Pathogenesis and transmission of SARS-CoV-2 in golden hamsters</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus with high nucleotide identity to SARS-CoV and to SARS-related coronaviruses that have been detected in horseshoe bats, has spread across the world and had a global effect on healthcare systems and economies
1
,
2
. A suitable small animal model is needed to support the development of vaccines and therapies. Here we report the pathogenesis and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters (
Mesocricetus auratus
). Immunohistochemistry assay demonstrated the presence of viral antigens in nasal mucosa, bronchial epithelial cells and areas of lung consolidation on days 2 and 5 after inoculation with SARS-CoV-2, followed by rapid viral clearance and pneumocyte hyperplasia at 7 days after inoculation. We also found viral antigens in epithelial cells of the duodenum, and detected viral RNA in faeces. Notably, SARS-CoV-2 was transmitted efficiently from inoculated hamsters to naive hamsters by direct contact and via aerosols. Transmission via fomites in soiled cages was not as efficient. Although viral RNA was continuously detected in the nasal washes of inoculated hamsters for 14 days, the communicable period was short and correlated with the detection of infectious virus but not viral RNA. Inoculated and naturally infected hamsters showed apparent weight loss on days 6–7 post-inoculation or post-contact; all hamsters returned to their original weight within 14 days and developed neutralizing antibodies. Our results suggest that features associated with SARS-CoV-2 infection in golden hamsters resemble those found in humans with mild SARS-CoV-2 infections.
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Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sia, Sin Fun</au><au>Yan, Li-Meng</au><au>Chin, Alex W. H.</au><au>Fung, Kevin</au><au>Choy, Ka-Tim</au><au>Wong, Alvina Y. L.</au><au>Kaewpreedee, Prathanporn</au><au>Perera, Ranawaka A. P. M.</au><au>Poon, Leo L. M.</au><au>Nicholls, John M.</au><au>Peiris, Malik</au><au>Yen, Hui-Ling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathogenesis and transmission of SARS-CoV-2 in golden hamsters</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>2020-07-30</date><risdate>2020</risdate><volume>583</volume><issue>7818</issue><spage>834</spage><epage>838</epage><pages>834-838</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus with high nucleotide identity to SARS-CoV and to SARS-related coronaviruses that have been detected in horseshoe bats, has spread across the world and had a global effect on healthcare systems and economies
1
,
2
. A suitable small animal model is needed to support the development of vaccines and therapies. Here we report the pathogenesis and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters (
Mesocricetus auratus
). Immunohistochemistry assay demonstrated the presence of viral antigens in nasal mucosa, bronchial epithelial cells and areas of lung consolidation on days 2 and 5 after inoculation with SARS-CoV-2, followed by rapid viral clearance and pneumocyte hyperplasia at 7 days after inoculation. We also found viral antigens in epithelial cells of the duodenum, and detected viral RNA in faeces. Notably, SARS-CoV-2 was transmitted efficiently from inoculated hamsters to naive hamsters by direct contact and via aerosols. Transmission via fomites in soiled cages was not as efficient. Although viral RNA was continuously detected in the nasal washes of inoculated hamsters for 14 days, the communicable period was short and correlated with the detection of infectious virus but not viral RNA. Inoculated and naturally infected hamsters showed apparent weight loss on days 6–7 post-inoculation or post-contact; all hamsters returned to their original weight within 14 days and developed neutralizing antibodies. Our results suggest that features associated with SARS-CoV-2 infection in golden hamsters resemble those found in humans with mild SARS-CoV-2 infections.
The pathogenicity and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters resemble features of COVID-19 in human patients, suggesting that these hamsters could be used to model this disease.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32408338</pmid><doi>10.1038/s41586-020-2342-5</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-8217-5995</orcidid><orcidid>https://orcid.org/0000-0003-2493-3609</orcidid><orcidid>https://orcid.org/0000-0002-0464-2401</orcidid><orcidid>https://orcid.org/0000-0003-3936-1535</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 2020-07, Vol.583 (7818), p.834-838 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2404039278 |
| source | MEDLINE; Nature; Alma/SFX Local Collection |
| subjects | 631/326/596/2555 631/326/596/2563 631/326/596/4130 64 Aerosols Alveolar Epithelial Cells - pathology Alveolar Epithelial Cells - virology Animal models Animals Antibodies Antibodies, Neutralizing - immunology Antibodies, Viral - immunology Antigens Antigens, Viral - immunology Antigens, Viral - isolation & purification Antigens, Viral - metabolism Betacoronavirus - immunology Betacoronavirus - isolation & purification Betacoronavirus - metabolism Betacoronavirus - pathogenicity Bronchi - pathology Bronchi - virology Coronaviridae Coronavirus Infections - immunology Coronavirus Infections - transmission Coronavirus Infections - virology Coronaviruses COVID-19 Disease Models, Animal Disease transmission Duodenum Duodenum - virology Epithelial cells Fomites Fomites - virology Hamsters Housing, Animal Humanities and Social Sciences Hyperplasia Immunization Immunohistochemistry Infections Influenza Inoculation Kidney - virology Lung - pathology Lung - virology Lungs Male Mesocricetus - immunology Mesocricetus - virology Mesocricetus auratus Mucosa multidisciplinary Nasal Mucosa - virology Neurons Nucleotides Pandemics Pathogenesis Pneumonia Pneumonia, Viral - immunology Pneumonia, Viral - transmission Pneumonia, Viral - virology Proteins Ribonucleic acid RNA RNA viruses RNA, Viral - analysis SARS-CoV-2 Science Science (multidisciplinary) Severe acute respiratory syndrome coronavirus 2 Transgenic animals Vaccines Viral diseases Viral Load Viruses Weight Loss |
| title | Pathogenesis and transmission of SARS-CoV-2 in golden hamsters |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T15%3A50%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pathogenesis%20and%20transmission%20of%20SARS-CoV-2%20in%20golden%20hamsters&rft.jtitle=Nature%20(London)&rft.au=Sia,%20Sin%20Fun&rft.date=2020-07-30&rft.volume=583&rft.issue=7818&rft.spage=834&rft.epage=838&rft.pages=834-838&rft.issn=0028-0836&rft.eissn=1476-4687&rft_id=info:doi/10.1038/s41586-020-2342-5&rft_dat=%3Cproquest_cross%3E2430412371%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2430412371&rft_id=info:pmid/32408338&rfr_iscdi=true |