Plasmidic Fluoroquinolone Resistance Genes in Fluoroquinolone-Resistant and/or Extended Spectrum Beta-Lactamase-Producing Escherichia coli Strains Isolated from Pediatric and Adult Patients Diagnosed with Urinary Tract Infection
Escherichia coli is the leading etiological agent of community-acquired urinary tract infection (UTI). Fluoroquinolones have long been the choice of empirical treatment for UTIs. Plasmid-mediated fluoroquinolone-resistance (PMFR) is important not only for conferring resistance to fluoroquinolones bu...
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| Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2020-11, Vol.26 (11), p.1334-1341 |
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| container_title | Microbial drug resistance (Larchmont, N.Y.) |
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| creator | Akgoz, Melisa Akman, Irem Ates, Asuman Begum Celik, Cem Keskin, Betul Ozmen Capin, Busra Betul Karahan, Zeynep Ceren |
| description | Escherichia coli
is the leading etiological agent of community-acquired urinary tract infection (UTI). Fluoroquinolones have long been the choice of empirical treatment for UTIs. Plasmid-mediated fluoroquinolone-resistance (PMFR) is important not only for conferring resistance to fluoroquinolones but also because of the presence of PMFR genes on plasmids carrying genes encoding resistance to other antimicrobials. In this study, we aimed at investigating the frequency of PMFR genes in fluoroquinolone-resistant and/or expanded spectrum beta-lactamase (ESBL)-producing
E. coli
strains isolated from pediatric and adult patients diagnosed with UTI.
E. coli
strains isolated from urine cultures of 141 adult and 117 pediatric outpatients were evaluated. Antimicrobial susceptibilities were interpreted according to the EUCAST criteria. The presence of PMFR genes (
qnrA, qnrB, qnrC, qnrS, qepA, aac(6′)-Ib,
and
aac(6′)-
Ib-cr) was investigated by multiplex PCR analysis. One hundred-three (73.05%) adult and 92 (78.63%) pediatric isolates were fluoroquinolone resistant and/or ESBL producers. One third (92/258) of all isolates carried at least one PMFR gene, the most prevalent one being
qnrS
(67.4%). None of the isolates carried
qnrC
and
qepA
genes. PMFR determinants were found to be widespread among adult and pediatric isolates. Rational antimicrobial use is crucial for prevention of resistance in both adult and pediatric populations. |
| doi_str_mv | 10.1089/mdr.2020.0007 |
| format | Article |
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is the leading etiological agent of community-acquired urinary tract infection (UTI). Fluoroquinolones have long been the choice of empirical treatment for UTIs. Plasmid-mediated fluoroquinolone-resistance (PMFR) is important not only for conferring resistance to fluoroquinolones but also because of the presence of PMFR genes on plasmids carrying genes encoding resistance to other antimicrobials. In this study, we aimed at investigating the frequency of PMFR genes in fluoroquinolone-resistant and/or expanded spectrum beta-lactamase (ESBL)-producing
E. coli
strains isolated from pediatric and adult patients diagnosed with UTI.
E. coli
strains isolated from urine cultures of 141 adult and 117 pediatric outpatients were evaluated. Antimicrobial susceptibilities were interpreted according to the EUCAST criteria. The presence of PMFR genes (
qnrA, qnrB, qnrC, qnrS, qepA, aac(6′)-Ib,
and
aac(6′)-
Ib-cr) was investigated by multiplex PCR analysis. One hundred-three (73.05%) adult and 92 (78.63%) pediatric isolates were fluoroquinolone resistant and/or ESBL producers. One third (92/258) of all isolates carried at least one PMFR gene, the most prevalent one being
qnrS
(67.4%). None of the isolates carried
qnrC
and
qepA
genes. PMFR determinants were found to be widespread among adult and pediatric isolates. Rational antimicrobial use is crucial for prevention of resistance in both adult and pediatric populations.</description><identifier>ISSN: 1076-6294</identifier><identifier>EISSN: 1931-8448</identifier><identifier>DOI: 10.1089/mdr.2020.0007</identifier><identifier>PMID: 32407158</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial agents ; E coli ; Empirical analysis ; Epidemiology ; Escherichia coli ; Etiology ; Fluoroquinolones ; Gene frequency ; Genes ; Patients ; Pediatrics ; Plasmids ; Urinary tract ; Urinary tract diseases ; Urinary tract infections ; Urogenital system ; β Lactamase</subject><ispartof>Microbial drug resistance (Larchmont, N.Y.), 2020-11, Vol.26 (11), p.1334-1341</ispartof><rights>2020, Mary Ann Liebert, Inc., publishers</rights><rights>Copyright Mary Ann Liebert, Inc. Nov 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-ac01e18189b4747c2d08b9acfc03aeeaaffed3ba1ec6e30d806f478c160343c03</citedby><cites>FETCH-LOGICAL-c404t-ac01e18189b4747c2d08b9acfc03aeeaaffed3ba1ec6e30d806f478c160343c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32407158$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akgoz, Melisa</creatorcontrib><creatorcontrib>Akman, Irem</creatorcontrib><creatorcontrib>Ates, Asuman Begum</creatorcontrib><creatorcontrib>Celik, Cem</creatorcontrib><creatorcontrib>Keskin, Betul</creatorcontrib><creatorcontrib>Ozmen Capin, Busra Betul</creatorcontrib><creatorcontrib>Karahan, Zeynep Ceren</creatorcontrib><title>Plasmidic Fluoroquinolone Resistance Genes in Fluoroquinolone-Resistant and/or Extended Spectrum Beta-Lactamase-Producing Escherichia coli Strains Isolated from Pediatric and Adult Patients Diagnosed with Urinary Tract Infection</title><title>Microbial drug resistance (Larchmont, N.Y.)</title><addtitle>Microb Drug Resist</addtitle><description>Escherichia coli
is the leading etiological agent of community-acquired urinary tract infection (UTI). Fluoroquinolones have long been the choice of empirical treatment for UTIs. Plasmid-mediated fluoroquinolone-resistance (PMFR) is important not only for conferring resistance to fluoroquinolones but also because of the presence of PMFR genes on plasmids carrying genes encoding resistance to other antimicrobials. In this study, we aimed at investigating the frequency of PMFR genes in fluoroquinolone-resistant and/or expanded spectrum beta-lactamase (ESBL)-producing
E. coli
strains isolated from pediatric and adult patients diagnosed with UTI.
E. coli
strains isolated from urine cultures of 141 adult and 117 pediatric outpatients were evaluated. Antimicrobial susceptibilities were interpreted according to the EUCAST criteria. The presence of PMFR genes (
qnrA, qnrB, qnrC, qnrS, qepA, aac(6′)-Ib,
and
aac(6′)-
Ib-cr) was investigated by multiplex PCR analysis. One hundred-three (73.05%) adult and 92 (78.63%) pediatric isolates were fluoroquinolone resistant and/or ESBL producers. One third (92/258) of all isolates carried at least one PMFR gene, the most prevalent one being
qnrS
(67.4%). None of the isolates carried
qnrC
and
qepA
genes. PMFR determinants were found to be widespread among adult and pediatric isolates. Rational antimicrobial use is crucial for prevention of resistance in both adult and pediatric populations.</description><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>E coli</subject><subject>Empirical analysis</subject><subject>Epidemiology</subject><subject>Escherichia coli</subject><subject>Etiology</subject><subject>Fluoroquinolones</subject><subject>Gene frequency</subject><subject>Genes</subject><subject>Patients</subject><subject>Pediatrics</subject><subject>Plasmids</subject><subject>Urinary tract</subject><subject>Urinary tract diseases</subject><subject>Urinary tract infections</subject><subject>Urogenital system</subject><subject>β Lactamase</subject><issn>1076-6294</issn><issn>1931-8448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqF0U1v3CAQBmCratWkaY-9Vki95OINGNbGxzTdpCutlFU-ztYYxlkiDFvAavp_-0OKtUkPuZQLCD0ahnmL4jOjC0ZlezbqsKhoRReU0uZNccxazkophHybz7Spy7pqxVHxIcbHLJas5u-LI14J2rClPC7-bC3E0WijyKWdfPA_J-O89Q7JDUYTEziF5AodRmLca1O-mETA6TMfyOopodOoye0eVQrTSL5hgnIDKsEIEctt8HpSxj2QVVQ7DEbtDBDlrSG3KYBxkayjt5ByjSH4kWxRG0jZzU-Qcz3ZRLaQDLoUyXcDD87HbH-ZtCP3wTgIv8ldyO-RtRtyD8a7j8W7AWzET8_7SXF_ubq7-FFurq_WF-ebUgkqUgmKMmSSybYXjWhUpansW1CDohwQAYYBNe-BoaqRUy1pPYhGKlZTLnhGJ8Xpoe5-nhHG1I0mKrQWHPopdnnqgnKZV6ZfX9FHPwWXu8tqWYtasEpkVR6UCj7GgEO3D2bMP-wY7eb4uxx_N8ffzfFn_-W56tSPqP_pl7wz4AcwX4Nz1mCPIf2n7F9DVMLF</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Akgoz, Melisa</creator><creator>Akman, Irem</creator><creator>Ates, Asuman Begum</creator><creator>Celik, Cem</creator><creator>Keskin, Betul</creator><creator>Ozmen Capin, Busra Betul</creator><creator>Karahan, Zeynep Ceren</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20201101</creationdate><title>Plasmidic Fluoroquinolone Resistance Genes in Fluoroquinolone-Resistant and/or Extended Spectrum Beta-Lactamase-Producing Escherichia coli Strains Isolated from Pediatric and Adult Patients Diagnosed with Urinary Tract Infection</title><author>Akgoz, Melisa ; Akman, Irem ; Ates, Asuman Begum ; Celik, Cem ; Keskin, Betul ; Ozmen Capin, Busra Betul ; Karahan, Zeynep Ceren</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-ac01e18189b4747c2d08b9acfc03aeeaaffed3ba1ec6e30d806f478c160343c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>E coli</topic><topic>Empirical analysis</topic><topic>Epidemiology</topic><topic>Escherichia coli</topic><topic>Etiology</topic><topic>Fluoroquinolones</topic><topic>Gene frequency</topic><topic>Genes</topic><topic>Patients</topic><topic>Pediatrics</topic><topic>Plasmids</topic><topic>Urinary tract</topic><topic>Urinary tract diseases</topic><topic>Urinary tract infections</topic><topic>Urogenital system</topic><topic>β Lactamase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akgoz, Melisa</creatorcontrib><creatorcontrib>Akman, Irem</creatorcontrib><creatorcontrib>Ates, Asuman Begum</creatorcontrib><creatorcontrib>Celik, Cem</creatorcontrib><creatorcontrib>Keskin, Betul</creatorcontrib><creatorcontrib>Ozmen Capin, Busra Betul</creatorcontrib><creatorcontrib>Karahan, Zeynep Ceren</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akgoz, Melisa</au><au>Akman, Irem</au><au>Ates, Asuman Begum</au><au>Celik, Cem</au><au>Keskin, Betul</au><au>Ozmen Capin, Busra Betul</au><au>Karahan, Zeynep Ceren</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasmidic Fluoroquinolone Resistance Genes in Fluoroquinolone-Resistant and/or Extended Spectrum Beta-Lactamase-Producing Escherichia coli Strains Isolated from Pediatric and Adult Patients Diagnosed with Urinary Tract Infection</atitle><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle><addtitle>Microb Drug Resist</addtitle><date>2020-11-01</date><risdate>2020</risdate><volume>26</volume><issue>11</issue><spage>1334</spage><epage>1341</epage><pages>1334-1341</pages><issn>1076-6294</issn><eissn>1931-8448</eissn><abstract>Escherichia coli
is the leading etiological agent of community-acquired urinary tract infection (UTI). Fluoroquinolones have long been the choice of empirical treatment for UTIs. Plasmid-mediated fluoroquinolone-resistance (PMFR) is important not only for conferring resistance to fluoroquinolones but also because of the presence of PMFR genes on plasmids carrying genes encoding resistance to other antimicrobials. In this study, we aimed at investigating the frequency of PMFR genes in fluoroquinolone-resistant and/or expanded spectrum beta-lactamase (ESBL)-producing
E. coli
strains isolated from pediatric and adult patients diagnosed with UTI.
E. coli
strains isolated from urine cultures of 141 adult and 117 pediatric outpatients were evaluated. Antimicrobial susceptibilities were interpreted according to the EUCAST criteria. The presence of PMFR genes (
qnrA, qnrB, qnrC, qnrS, qepA, aac(6′)-Ib,
and
aac(6′)-
Ib-cr) was investigated by multiplex PCR analysis. One hundred-three (73.05%) adult and 92 (78.63%) pediatric isolates were fluoroquinolone resistant and/or ESBL producers. One third (92/258) of all isolates carried at least one PMFR gene, the most prevalent one being
qnrS
(67.4%). None of the isolates carried
qnrC
and
qepA
genes. PMFR determinants were found to be widespread among adult and pediatric isolates. Rational antimicrobial use is crucial for prevention of resistance in both adult and pediatric populations.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>32407158</pmid><doi>10.1089/mdr.2020.0007</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1076-6294 |
| ispartof | Microbial drug resistance (Larchmont, N.Y.), 2020-11, Vol.26 (11), p.1334-1341 |
| issn | 1076-6294 1931-8448 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2404038888 |
| source | Alma/SFX Local Collection |
| subjects | Antibiotics Antiinfectives and antibacterials Antimicrobial agents E coli Empirical analysis Epidemiology Escherichia coli Etiology Fluoroquinolones Gene frequency Genes Patients Pediatrics Plasmids Urinary tract Urinary tract diseases Urinary tract infections Urogenital system β Lactamase |
| title | Plasmidic Fluoroquinolone Resistance Genes in Fluoroquinolone-Resistant and/or Extended Spectrum Beta-Lactamase-Producing Escherichia coli Strains Isolated from Pediatric and Adult Patients Diagnosed with Urinary Tract Infection |
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