Vascular activity of infusion and fractions of Cymbopogon citratus (DC) Stapf. in human arteries
Cymbopogon citratus (DC.) Stapf has been traditionally used mainly for inflammatory diseases and hypertension. However, the mechanisms underlying its vascular activity remain to be fully characterized and the fractions responsible for its cardiovascular activity are still unknown. In this study, we...
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| Veröffentlicht in: | Journal of ethnopharmacology 2020-08, Vol.258, p.112947-112947, Article 112947 |
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| creator | Simões, Daniela M. Malheiros, Jéssica Antunes, Pedro E. Figueirinha, Artur Cotrim, Maria Dulce Fonseca, Diogo A. |
| description | Cymbopogon citratus (DC.) Stapf has been traditionally used mainly for inflammatory diseases and hypertension. However, the mechanisms underlying its vascular activity remain to be fully characterized and the fractions responsible for its cardiovascular activity are still unknown.
In this study, we aimed to assess the vascular activity of Cymbopogon citratus in human arteries and to study the role of cyclooxygenase in its vasorelaxant effects.
Vascular effects of leaves infusion and three fractions (phenolic acids, flavonoids and tannins) were studied using distal segments of human internal thoracic arteries harvested from patients undergoing coronary revascularization, which were mounted as rings in tissue organ baths and maintained at 37 °C in Krebs Henseleit buffer. The effect on basal vascular tone, the effect on the noradrenaline-induced contraction and the vasorelaxant effects were assessed. The role of cyclooxygenase was evaluated with indomethacin.
Our results showed a mild effect on the basal vessel tone of the infusion. A significant inhibition on the adrenergic-mediated vasoconstriction was observed for the infusion (0.0002 mg/mL) and the flavonoid fraction (0.2 mg/mL), despite a potentiation was observed in some conditions. A vasorelaxant effect was observed for both the infusion (6.46% of maximal relaxation) and the tannin fraction (26.91% of maximal relaxation, P |
| doi_str_mv | 10.1016/j.jep.2020.112947 |
| format | Article |
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In this study, we aimed to assess the vascular activity of Cymbopogon citratus in human arteries and to study the role of cyclooxygenase in its vasorelaxant effects.
Vascular effects of leaves infusion and three fractions (phenolic acids, flavonoids and tannins) were studied using distal segments of human internal thoracic arteries harvested from patients undergoing coronary revascularization, which were mounted as rings in tissue organ baths and maintained at 37 °C in Krebs Henseleit buffer. The effect on basal vascular tone, the effect on the noradrenaline-induced contraction and the vasorelaxant effects were assessed. The role of cyclooxygenase was evaluated with indomethacin.
Our results showed a mild effect on the basal vessel tone of the infusion. A significant inhibition on the adrenergic-mediated vasoconstriction was observed for the infusion (0.0002 mg/mL) and the flavonoid fraction (0.2 mg/mL), despite a potentiation was observed in some conditions. A vasorelaxant effect was observed for both the infusion (6.46% of maximal relaxation) and the tannin fraction (26.91% of maximal relaxation, P < 0.05 vs. infusion). Incubation with indomethacin (10 μM) elicited a decrease in the vasorelaxation to the infusion (P < 0.05).
These results suggest that cyclooxygenase may be involved in the vasorelaxation to the infusion of Cymbopogon citratus and that tannins are the compound fraction mainly responsible for this vasorelaxation.
[Display omitted]</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2020.112947</identifier><identifier>PMID: 32387462</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Cyclooxygenase ; Cymbopogon - chemistry ; Cymbopogon citratus ; Flavonoids - isolation & purification ; Flavonoids - pharmacology ; Human internal thoracic artery ; Humans ; Hydroxybenzoates - isolation & purification ; Hydroxybenzoates - pharmacology ; Indomethacin - pharmacology ; Infusion ; Mammary Arteries - drug effects ; Mammary Arteries - metabolism ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Leaves ; Prostaglandin-Endoperoxide Synthases - drug effects ; Prostaglandin-Endoperoxide Synthases - metabolism ; Tannins ; Tannins - isolation & purification ; Tannins - pharmacology ; Vascular effects ; Vasodilator Agents - isolation & purification ; Vasodilator Agents - pharmacology</subject><ispartof>Journal of ethnopharmacology, 2020-08, Vol.258, p.112947-112947, Article 112947</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-1e2162dc931b033169e80acb3a8fbd9b3449fe7d58ac438c43de4eff53520aa33</citedby><cites>FETCH-LOGICAL-c353t-1e2162dc931b033169e80acb3a8fbd9b3449fe7d58ac438c43de4eff53520aa33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jep.2020.112947$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32387462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simões, Daniela M.</creatorcontrib><creatorcontrib>Malheiros, Jéssica</creatorcontrib><creatorcontrib>Antunes, Pedro E.</creatorcontrib><creatorcontrib>Figueirinha, Artur</creatorcontrib><creatorcontrib>Cotrim, Maria Dulce</creatorcontrib><creatorcontrib>Fonseca, Diogo A.</creatorcontrib><title>Vascular activity of infusion and fractions of Cymbopogon citratus (DC) Stapf. in human arteries</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Cymbopogon citratus (DC.) Stapf has been traditionally used mainly for inflammatory diseases and hypertension. However, the mechanisms underlying its vascular activity remain to be fully characterized and the fractions responsible for its cardiovascular activity are still unknown.
In this study, we aimed to assess the vascular activity of Cymbopogon citratus in human arteries and to study the role of cyclooxygenase in its vasorelaxant effects.
Vascular effects of leaves infusion and three fractions (phenolic acids, flavonoids and tannins) were studied using distal segments of human internal thoracic arteries harvested from patients undergoing coronary revascularization, which were mounted as rings in tissue organ baths and maintained at 37 °C in Krebs Henseleit buffer. The effect on basal vascular tone, the effect on the noradrenaline-induced contraction and the vasorelaxant effects were assessed. The role of cyclooxygenase was evaluated with indomethacin.
Our results showed a mild effect on the basal vessel tone of the infusion. A significant inhibition on the adrenergic-mediated vasoconstriction was observed for the infusion (0.0002 mg/mL) and the flavonoid fraction (0.2 mg/mL), despite a potentiation was observed in some conditions. A vasorelaxant effect was observed for both the infusion (6.46% of maximal relaxation) and the tannin fraction (26.91% of maximal relaxation, P < 0.05 vs. infusion). Incubation with indomethacin (10 μM) elicited a decrease in the vasorelaxation to the infusion (P < 0.05).
These results suggest that cyclooxygenase may be involved in the vasorelaxation to the infusion of Cymbopogon citratus and that tannins are the compound fraction mainly responsible for this vasorelaxation.
[Display omitted]</description><subject>Cyclooxygenase</subject><subject>Cymbopogon - chemistry</subject><subject>Cymbopogon citratus</subject><subject>Flavonoids - isolation & purification</subject><subject>Flavonoids - pharmacology</subject><subject>Human internal thoracic artery</subject><subject>Humans</subject><subject>Hydroxybenzoates - isolation & purification</subject><subject>Hydroxybenzoates - pharmacology</subject><subject>Indomethacin - pharmacology</subject><subject>Infusion</subject><subject>Mammary Arteries - drug effects</subject><subject>Mammary Arteries - metabolism</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves</subject><subject>Prostaglandin-Endoperoxide Synthases - drug effects</subject><subject>Prostaglandin-Endoperoxide Synthases - metabolism</subject><subject>Tannins</subject><subject>Tannins - isolation & purification</subject><subject>Tannins - pharmacology</subject><subject>Vascular effects</subject><subject>Vasodilator Agents - isolation & purification</subject><subject>Vasodilator Agents - pharmacology</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlOwzAQhi0EoqXwAFxQjuWQ4i11Ik6orBISB5arcZwxuGriYDtIfXtctXDkMLLG_yLNh9ApwTOCyfxiOVtCP6OYpp3Qios9NCaloLkoBNtHY8xEmZeCkxE6CmGJMRaE40M0YpSl7zkdo_c3FfSwUj5TOtpvG9eZM5ntzBCs6zLVNZnxG8l1YaMs1m3teveRNG2jV3EI2fR6cZ49R9WbWUpmn0OrUtJH8BbCMTowahXgZPdO0OvtzcviPn98untYXD3mmhUs5gQomdNGV4zUmDEyr6DEStdMlaZuqppxXhkQTVEqzVmZpgEOxhSsoFgpxiZouu3tvfsaIETZ2qBhtVIduCFIyjEhjAhOk5Vsrdq7EDwY2XvbKr-WBMsNWLmUCazcgJVbsClztqsf6haav8QvyWS43BogHfltwcugLXQaGutBR9k4-0_9DwtYiMc</recordid><startdate>20200810</startdate><enddate>20200810</enddate><creator>Simões, Daniela M.</creator><creator>Malheiros, Jéssica</creator><creator>Antunes, Pedro E.</creator><creator>Figueirinha, Artur</creator><creator>Cotrim, Maria Dulce</creator><creator>Fonseca, Diogo A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200810</creationdate><title>Vascular activity of infusion and fractions of Cymbopogon citratus (DC) Stapf. in human arteries</title><author>Simões, Daniela M. ; Malheiros, Jéssica ; Antunes, Pedro E. ; Figueirinha, Artur ; Cotrim, Maria Dulce ; Fonseca, Diogo A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-1e2162dc931b033169e80acb3a8fbd9b3449fe7d58ac438c43de4eff53520aa33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Cyclooxygenase</topic><topic>Cymbopogon - chemistry</topic><topic>Cymbopogon citratus</topic><topic>Flavonoids - isolation & purification</topic><topic>Flavonoids - pharmacology</topic><topic>Human internal thoracic artery</topic><topic>Humans</topic><topic>Hydroxybenzoates - isolation & purification</topic><topic>Hydroxybenzoates - pharmacology</topic><topic>Indomethacin - pharmacology</topic><topic>Infusion</topic><topic>Mammary Arteries - drug effects</topic><topic>Mammary Arteries - metabolism</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves</topic><topic>Prostaglandin-Endoperoxide Synthases - drug effects</topic><topic>Prostaglandin-Endoperoxide Synthases - metabolism</topic><topic>Tannins</topic><topic>Tannins - isolation & purification</topic><topic>Tannins - pharmacology</topic><topic>Vascular effects</topic><topic>Vasodilator Agents - isolation & purification</topic><topic>Vasodilator Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simões, Daniela M.</creatorcontrib><creatorcontrib>Malheiros, Jéssica</creatorcontrib><creatorcontrib>Antunes, Pedro E.</creatorcontrib><creatorcontrib>Figueirinha, Artur</creatorcontrib><creatorcontrib>Cotrim, Maria Dulce</creatorcontrib><creatorcontrib>Fonseca, Diogo A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simões, Daniela M.</au><au>Malheiros, Jéssica</au><au>Antunes, Pedro E.</au><au>Figueirinha, Artur</au><au>Cotrim, Maria Dulce</au><au>Fonseca, Diogo A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vascular activity of infusion and fractions of Cymbopogon citratus (DC) Stapf. in human arteries</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2020-08-10</date><risdate>2020</risdate><volume>258</volume><spage>112947</spage><epage>112947</epage><pages>112947-112947</pages><artnum>112947</artnum><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Cymbopogon citratus (DC.) Stapf has been traditionally used mainly for inflammatory diseases and hypertension. However, the mechanisms underlying its vascular activity remain to be fully characterized and the fractions responsible for its cardiovascular activity are still unknown.
In this study, we aimed to assess the vascular activity of Cymbopogon citratus in human arteries and to study the role of cyclooxygenase in its vasorelaxant effects.
Vascular effects of leaves infusion and three fractions (phenolic acids, flavonoids and tannins) were studied using distal segments of human internal thoracic arteries harvested from patients undergoing coronary revascularization, which were mounted as rings in tissue organ baths and maintained at 37 °C in Krebs Henseleit buffer. The effect on basal vascular tone, the effect on the noradrenaline-induced contraction and the vasorelaxant effects were assessed. The role of cyclooxygenase was evaluated with indomethacin.
Our results showed a mild effect on the basal vessel tone of the infusion. A significant inhibition on the adrenergic-mediated vasoconstriction was observed for the infusion (0.0002 mg/mL) and the flavonoid fraction (0.2 mg/mL), despite a potentiation was observed in some conditions. A vasorelaxant effect was observed for both the infusion (6.46% of maximal relaxation) and the tannin fraction (26.91% of maximal relaxation, P < 0.05 vs. infusion). Incubation with indomethacin (10 μM) elicited a decrease in the vasorelaxation to the infusion (P < 0.05).
These results suggest that cyclooxygenase may be involved in the vasorelaxation to the infusion of Cymbopogon citratus and that tannins are the compound fraction mainly responsible for this vasorelaxation.
[Display omitted]</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>32387462</pmid><doi>10.1016/j.jep.2020.112947</doi><tpages>1</tpages></addata></record> |
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| subjects | Cyclooxygenase Cymbopogon - chemistry Cymbopogon citratus Flavonoids - isolation & purification Flavonoids - pharmacology Human internal thoracic artery Humans Hydroxybenzoates - isolation & purification Hydroxybenzoates - pharmacology Indomethacin - pharmacology Infusion Mammary Arteries - drug effects Mammary Arteries - metabolism Plant Extracts - chemistry Plant Extracts - pharmacology Plant Leaves Prostaglandin-Endoperoxide Synthases - drug effects Prostaglandin-Endoperoxide Synthases - metabolism Tannins Tannins - isolation & purification Tannins - pharmacology Vascular effects Vasodilator Agents - isolation & purification Vasodilator Agents - pharmacology |
| title | Vascular activity of infusion and fractions of Cymbopogon citratus (DC) Stapf. in human arteries |
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