A rapid chair‐side method for the estimation of oral bacterial colonization density
Aims Caries and periodontal disease are associated with inadequate control of oral bacteria. Since conventional microbiological evaluations are impractical in dental clinics or public engagement activities, a rapid test for the quantification of oral bacteria represents a useful tool. We describe th...
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| Veröffentlicht in: | Journal of applied microbiology 2020-03, Vol.128 (3), p.884-892 |
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| container_title | Journal of applied microbiology |
| container_volume | 128 |
| creator | Ledder, R.G. Latimer, J. Buzza, K.M. Haddad, G.S. Wilson, R.A. Sreenivasan, P.K. McBain, A.J. |
| description | Aims
Caries and periodontal disease are associated with inadequate control of oral bacteria. Since conventional microbiological evaluations are impractical in dental clinics or public engagement activities, a rapid test for the quantification of oral bacteria represents a useful tool. We describe the development of a colour change test to rapidly estimate bacterial colonisation density in the mouth.
Methods and Results
Volunteers rinsed with milk or milkshake. Viability indicators were added and colour changes quantified during incubation. Using milkshake and the resazurin‐based solution PrestoBlue (9% v/v), the method distinguished between samples before and after brushing within 5 min. Colour changes were quantified and viable counts were obtained using oral rinses. Measured colour changes strongly correlated with total counts of both anaerobes and streptococci (Spearman’s correlation coefficient of 0·782 and 0·769, respectively, P ≤ 0·001) and with perceived changes, as determined by volunteers (n = 10) visually ranking images.
Conclusions
The resazurin milkshake test can rapidly and visually quantify viable bacteria in oral samples.
Significance and Impact of the Study
The resazurin milkshake test could serve as a sensitive semi‐quantitative method for measuring oral bacteria in human oral rinse samples. |
| doi_str_mv | 10.1111/jam.14529 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2400490602</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2353365172</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3819-e2fd1e609a6a1dce9f0ee1e2f5c8257fbab9c2eb0d043acebe519db487aa15123</originalsourceid><addsrcrecordid>eNqNkU9LwzAYh4Mobk4PfgEJeNFDXZI27XIcw79MvLhzSZO3LKNtZtIh8-RH8DP6SczW6UEQzCUvycND8vshdErJFQ1ruJD1FU04E3uoT-OURyzN2P52TiJOMtZDR94vCKEx4ekh6sU046MsTvpoNsZOLo3Gai6N-3z_8EYDrqGdW41L63A7Bwy-NbVsjW2wLbF1ssKFVC04EyZlK9uYt-5aQ-NNuz5GB6WsPJzs9gGa3Vw_T-6i6dPt_WQ8jVQ8oiICVmoKKREylVQrECUBoOGUqxHjWVnIQigGBdEkiaWCAjgVukhGmZSUUxYP0EXnXTr7sgrPzGvjFVSVbMCufM4SQhJBUvIPdJOJECGUgJ7_Qhd25ZrwkUDxOARMs43wsqOUs947KPOlCym5dU5JvqklD7Xk21oCe7Yzrooa9A_53UMAhh3waipY_23KH8aPnfILIL2XfQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2353365172</pqid></control><display><type>article</type><title>A rapid chair‐side method for the estimation of oral bacterial colonization density</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Ledder, R.G. ; Latimer, J. ; Buzza, K.M. ; Haddad, G.S. ; Wilson, R.A. ; Sreenivasan, P.K. ; McBain, A.J.</creator><creatorcontrib>Ledder, R.G. ; Latimer, J. ; Buzza, K.M. ; Haddad, G.S. ; Wilson, R.A. ; Sreenivasan, P.K. ; McBain, A.J.</creatorcontrib><description>Aims
Caries and periodontal disease are associated with inadequate control of oral bacteria. Since conventional microbiological evaluations are impractical in dental clinics or public engagement activities, a rapid test for the quantification of oral bacteria represents a useful tool. We describe the development of a colour change test to rapidly estimate bacterial colonisation density in the mouth.
Methods and Results
Volunteers rinsed with milk or milkshake. Viability indicators were added and colour changes quantified during incubation. Using milkshake and the resazurin‐based solution PrestoBlue (9% v/v), the method distinguished between samples before and after brushing within 5 min. Colour changes were quantified and viable counts were obtained using oral rinses. Measured colour changes strongly correlated with total counts of both anaerobes and streptococci (Spearman’s correlation coefficient of 0·782 and 0·769, respectively, P ≤ 0·001) and with perceived changes, as determined by volunteers (n = 10) visually ranking images.
Conclusions
The resazurin milkshake test can rapidly and visually quantify viable bacteria in oral samples.
Significance and Impact of the Study
The resazurin milkshake test could serve as a sensitive semi‐quantitative method for measuring oral bacteria in human oral rinse samples.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14529</identifier><identifier>PMID: 31758734</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adult ; Anaerobes ; Bacteria ; Bacteria - isolation & purification ; bacterial colonization ; Bacterial Load - methods ; caries ; citizen participation ; Colonization ; Colony Count, Microbial ; Color ; Correlation analysis ; Correlation coefficient ; Correlation coefficients ; Density ; Dental caries ; Disease control ; education ; gingival disease ; Humans ; Indicators and Reagents ; Male ; Milk ; milkshakes ; mouth ; Mouth - microbiology ; oral health ; Oxazines ; Periodontal disease ; Periodontal diseases ; plate count ; Point-of-Care Testing ; point‐of‐care ; Public participation ; rapid methods ; Streptococcus ; Viability ; volunteers ; Xanthenes</subject><ispartof>Journal of applied microbiology, 2020-03, Vol.128 (3), p.884-892</ispartof><rights>2019 The Society for Applied Microbiology</rights><rights>2019 The Society for Applied Microbiology.</rights><rights>Copyright © 2020 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3819-e2fd1e609a6a1dce9f0ee1e2f5c8257fbab9c2eb0d043acebe519db487aa15123</cites><orcidid>0000-0001-7613-6607 ; 0000-0002-5255-5425</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14529$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14529$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31758734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ledder, R.G.</creatorcontrib><creatorcontrib>Latimer, J.</creatorcontrib><creatorcontrib>Buzza, K.M.</creatorcontrib><creatorcontrib>Haddad, G.S.</creatorcontrib><creatorcontrib>Wilson, R.A.</creatorcontrib><creatorcontrib>Sreenivasan, P.K.</creatorcontrib><creatorcontrib>McBain, A.J.</creatorcontrib><title>A rapid chair‐side method for the estimation of oral bacterial colonization density</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
Caries and periodontal disease are associated with inadequate control of oral bacteria. Since conventional microbiological evaluations are impractical in dental clinics or public engagement activities, a rapid test for the quantification of oral bacteria represents a useful tool. We describe the development of a colour change test to rapidly estimate bacterial colonisation density in the mouth.
Methods and Results
Volunteers rinsed with milk or milkshake. Viability indicators were added and colour changes quantified during incubation. Using milkshake and the resazurin‐based solution PrestoBlue (9% v/v), the method distinguished between samples before and after brushing within 5 min. Colour changes were quantified and viable counts were obtained using oral rinses. Measured colour changes strongly correlated with total counts of both anaerobes and streptococci (Spearman’s correlation coefficient of 0·782 and 0·769, respectively, P ≤ 0·001) and with perceived changes, as determined by volunteers (n = 10) visually ranking images.
Conclusions
The resazurin milkshake test can rapidly and visually quantify viable bacteria in oral samples.
Significance and Impact of the Study
The resazurin milkshake test could serve as a sensitive semi‐quantitative method for measuring oral bacteria in human oral rinse samples.</description><subject>Adult</subject><subject>Anaerobes</subject><subject>Bacteria</subject><subject>Bacteria - isolation & purification</subject><subject>bacterial colonization</subject><subject>Bacterial Load - methods</subject><subject>caries</subject><subject>citizen participation</subject><subject>Colonization</subject><subject>Colony Count, Microbial</subject><subject>Color</subject><subject>Correlation analysis</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Density</subject><subject>Dental caries</subject><subject>Disease control</subject><subject>education</subject><subject>gingival disease</subject><subject>Humans</subject><subject>Indicators and Reagents</subject><subject>Male</subject><subject>Milk</subject><subject>milkshakes</subject><subject>mouth</subject><subject>Mouth - microbiology</subject><subject>oral health</subject><subject>Oxazines</subject><subject>Periodontal disease</subject><subject>Periodontal diseases</subject><subject>plate count</subject><subject>Point-of-Care Testing</subject><subject>point‐of‐care</subject><subject>Public participation</subject><subject>rapid methods</subject><subject>Streptococcus</subject><subject>Viability</subject><subject>volunteers</subject><subject>Xanthenes</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9LwzAYh4Mobk4PfgEJeNFDXZI27XIcw79MvLhzSZO3LKNtZtIh8-RH8DP6SczW6UEQzCUvycND8vshdErJFQ1ruJD1FU04E3uoT-OURyzN2P52TiJOMtZDR94vCKEx4ekh6sU046MsTvpoNsZOLo3Gai6N-3z_8EYDrqGdW41L63A7Bwy-NbVsjW2wLbF1ssKFVC04EyZlK9uYt-5aQ-NNuz5GB6WsPJzs9gGa3Vw_T-6i6dPt_WQ8jVQ8oiICVmoKKREylVQrECUBoOGUqxHjWVnIQigGBdEkiaWCAjgVukhGmZSUUxYP0EXnXTr7sgrPzGvjFVSVbMCufM4SQhJBUvIPdJOJECGUgJ7_Qhd25ZrwkUDxOARMs43wsqOUs947KPOlCym5dU5JvqklD7Xk21oCe7Yzrooa9A_53UMAhh3waipY_23KH8aPnfILIL2XfQ</recordid><startdate>202003</startdate><enddate>202003</enddate><creator>Ledder, R.G.</creator><creator>Latimer, J.</creator><creator>Buzza, K.M.</creator><creator>Haddad, G.S.</creator><creator>Wilson, R.A.</creator><creator>Sreenivasan, P.K.</creator><creator>McBain, A.J.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-7613-6607</orcidid><orcidid>https://orcid.org/0000-0002-5255-5425</orcidid></search><sort><creationdate>202003</creationdate><title>A rapid chair‐side method for the estimation of oral bacterial colonization density</title><author>Ledder, R.G. ; Latimer, J. ; Buzza, K.M. ; Haddad, G.S. ; Wilson, R.A. ; Sreenivasan, P.K. ; McBain, A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3819-e2fd1e609a6a1dce9f0ee1e2f5c8257fbab9c2eb0d043acebe519db487aa15123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adult</topic><topic>Anaerobes</topic><topic>Bacteria</topic><topic>Bacteria - isolation & purification</topic><topic>bacterial colonization</topic><topic>Bacterial Load - methods</topic><topic>caries</topic><topic>citizen participation</topic><topic>Colonization</topic><topic>Colony Count, Microbial</topic><topic>Color</topic><topic>Correlation analysis</topic><topic>Correlation coefficient</topic><topic>Correlation coefficients</topic><topic>Density</topic><topic>Dental caries</topic><topic>Disease control</topic><topic>education</topic><topic>gingival disease</topic><topic>Humans</topic><topic>Indicators and Reagents</topic><topic>Male</topic><topic>Milk</topic><topic>milkshakes</topic><topic>mouth</topic><topic>Mouth - microbiology</topic><topic>oral health</topic><topic>Oxazines</topic><topic>Periodontal disease</topic><topic>Periodontal diseases</topic><topic>plate count</topic><topic>Point-of-Care Testing</topic><topic>point‐of‐care</topic><topic>Public participation</topic><topic>rapid methods</topic><topic>Streptococcus</topic><topic>Viability</topic><topic>volunteers</topic><topic>Xanthenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ledder, R.G.</creatorcontrib><creatorcontrib>Latimer, J.</creatorcontrib><creatorcontrib>Buzza, K.M.</creatorcontrib><creatorcontrib>Haddad, G.S.</creatorcontrib><creatorcontrib>Wilson, R.A.</creatorcontrib><creatorcontrib>Sreenivasan, P.K.</creatorcontrib><creatorcontrib>McBain, A.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ledder, R.G.</au><au>Latimer, J.</au><au>Buzza, K.M.</au><au>Haddad, G.S.</au><au>Wilson, R.A.</au><au>Sreenivasan, P.K.</au><au>McBain, A.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid chair‐side method for the estimation of oral bacterial colonization density</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2020-03</date><risdate>2020</risdate><volume>128</volume><issue>3</issue><spage>884</spage><epage>892</epage><pages>884-892</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
Caries and periodontal disease are associated with inadequate control of oral bacteria. Since conventional microbiological evaluations are impractical in dental clinics or public engagement activities, a rapid test for the quantification of oral bacteria represents a useful tool. We describe the development of a colour change test to rapidly estimate bacterial colonisation density in the mouth.
Methods and Results
Volunteers rinsed with milk or milkshake. Viability indicators were added and colour changes quantified during incubation. Using milkshake and the resazurin‐based solution PrestoBlue (9% v/v), the method distinguished between samples before and after brushing within 5 min. Colour changes were quantified and viable counts were obtained using oral rinses. Measured colour changes strongly correlated with total counts of both anaerobes and streptococci (Spearman’s correlation coefficient of 0·782 and 0·769, respectively, P ≤ 0·001) and with perceived changes, as determined by volunteers (n = 10) visually ranking images.
Conclusions
The resazurin milkshake test can rapidly and visually quantify viable bacteria in oral samples.
Significance and Impact of the Study
The resazurin milkshake test could serve as a sensitive semi‐quantitative method for measuring oral bacteria in human oral rinse samples.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>31758734</pmid><doi>10.1111/jam.14529</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7613-6607</orcidid><orcidid>https://orcid.org/0000-0002-5255-5425</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1364-5072 |
| ispartof | Journal of applied microbiology, 2020-03, Vol.128 (3), p.884-892 |
| issn | 1364-5072 1365-2672 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adult Anaerobes Bacteria Bacteria - isolation & purification bacterial colonization Bacterial Load - methods caries citizen participation Colonization Colony Count, Microbial Color Correlation analysis Correlation coefficient Correlation coefficients Density Dental caries Disease control education gingival disease Humans Indicators and Reagents Male Milk milkshakes mouth Mouth - microbiology oral health Oxazines Periodontal disease Periodontal diseases plate count Point-of-Care Testing point‐of‐care Public participation rapid methods Streptococcus Viability volunteers Xanthenes |
| title | A rapid chair‐side method for the estimation of oral bacterial colonization density |
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