Prevalence and genotype distribution of Enterocytozoon bieneusi in farmed raccoon dogs (Nyctereutes procyonoides) in Shandong Province, eastern China
Enterocytozoon bieneusi is a common microsporidian species, which can infect humans and various species of animals. However, little is known about E. bieneusi prevalence and genotypes in farmed raccoon dogs ( Nyctereutes procyonoides ) in Shandong Province, China. In this study, a total of 356 fecal...
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| Veröffentlicht in: | Parasitology research (1987) 2020-06, Vol.119 (6), p.1873-1878 |
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| container_title | Parasitology research (1987) |
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| creator | Ma, Yuan-Yuan Ma, Ye-Ting Nie, Lan-Bi Li, Tao-Shan Peng, Jun-Jie Cong, Wei Zou, Yang Zhu, Xing-Quan |
| description | Enterocytozoon bieneusi
is a common microsporidian species, which can infect humans and various species of animals. However, little is known about
E. bieneusi
prevalence and genotypes in farmed raccoon dogs (
Nyctereutes procyonoides
) in Shandong Province, China. In this study, a total of 356 fecal samples were collected from farmed raccoon dogs in Weihai, Weifang, and Yantai cities in Shandong Province, China. A total of 23 (6.5%) samples were
E. bieneusi
-positive by nested PCR amplification of the internal transcribed spacer (ITS) region of ribosomal DNA. Statistical analysis showed that
E. bieneusi
prevalence in male raccoon dogs was higher than that in female raccoon dogs, and the highest
E. bieneusi
prevalence was detected in adult raccoon dogs. Sequence analysis revealed four known
E. bieneusi
genotypes (D, type IV, CHG1, and Peru8), and type IV (11/23) was the predominant genotype. The genotypes type IV, Peru8, and CHG1 were reported in raccoon dogs for the first time in China. Phylogenetic analysis showed that three human-pathogenic genotypes (D, type IV, and Peru8) were clustered into group 1, and the CHG1 belonged to group 2. These findings expand the current understanding of
E. bieneusi
prevalence and genotype distribution in raccoon dogs in China. Our study also shows that raccoon dogs are hosts for
E. bieneusi
belonging to several genotypes, including zoonotic ones, highlighting the possibility of transmission of this pathogen between raccoon dogs and humans. |
| doi_str_mv | 10.1007/s00436-020-06693-5 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2399831666</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A625363866</galeid><sourcerecordid>A625363866</sourcerecordid><originalsourceid>FETCH-LOGICAL-c442t-6f4d10d42f6c248e2061ce4b1cfc035370e1d51c99b96f01049bd7e1ff57ef093</originalsourceid><addsrcrecordid>eNp9UtuKFDEQDaK44-oP-CABX1aw18ql05PHZVgvsOiC-hy6k8pslplkTLoXxv_wf03vrFdE8pBQdc6pStUh5CmDUwbQvSoAUqgGODSglBZNe48smBS8Ybpt75MF6PoGxsQReVTKNQDrlJQPyZHgous0dAvy7TLjTb_BaJH20dE1xjTud0hdKGMOwzSGFGny9DyOmJPdj-lrqpEhYMSpBBoi9X3eoqO5t3ZOubQu9OT93lYCTiMWupuJKabgsLyYGR-vaq0U1_Qyp5tQa7-k2JeKj3R1FWL_mDzw_abgk7v7mHx-ff5p9ba5-PDm3ersorFS8rFRXjoGTnKvLJdL5KCYRTkw6y2IVnSAzLXMaj1o5YGB1IPrkHnfdujrdI7JyUG3dvhlwjKabSgWN5s-YpqK4ULrpWBKqQp9_hf0Ok051u4Ml9AtFdfwG2pdZ2pC9GmsY5lFzZnirVBieat1-g9UPQ63waaIPtT4HwR-INicSsnozS6HbZ_3hoGZvWAOXjDVC-bWC6atpGd3HU9D3c9Pyo_lV4A4AEpNxTXmX1_6j-x33iK_6g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2407862906</pqid></control><display><type>article</type><title>Prevalence and genotype distribution of Enterocytozoon bieneusi in farmed raccoon dogs (Nyctereutes procyonoides) in Shandong Province, eastern China</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Ma, Yuan-Yuan ; Ma, Ye-Ting ; Nie, Lan-Bi ; Li, Tao-Shan ; Peng, Jun-Jie ; Cong, Wei ; Zou, Yang ; Zhu, Xing-Quan</creator><creatorcontrib>Ma, Yuan-Yuan ; Ma, Ye-Ting ; Nie, Lan-Bi ; Li, Tao-Shan ; Peng, Jun-Jie ; Cong, Wei ; Zou, Yang ; Zhu, Xing-Quan</creatorcontrib><description>Enterocytozoon bieneusi
is a common microsporidian species, which can infect humans and various species of animals. However, little is known about
E. bieneusi
prevalence and genotypes in farmed raccoon dogs (
Nyctereutes procyonoides
) in Shandong Province, China. In this study, a total of 356 fecal samples were collected from farmed raccoon dogs in Weihai, Weifang, and Yantai cities in Shandong Province, China. A total of 23 (6.5%) samples were
E. bieneusi
-positive by nested PCR amplification of the internal transcribed spacer (ITS) region of ribosomal DNA. Statistical analysis showed that
E. bieneusi
prevalence in male raccoon dogs was higher than that in female raccoon dogs, and the highest
E. bieneusi
prevalence was detected in adult raccoon dogs. Sequence analysis revealed four known
E. bieneusi
genotypes (D, type IV, CHG1, and Peru8), and type IV (11/23) was the predominant genotype. The genotypes type IV, Peru8, and CHG1 were reported in raccoon dogs for the first time in China. Phylogenetic analysis showed that three human-pathogenic genotypes (D, type IV, and Peru8) were clustered into group 1, and the CHG1 belonged to group 2. These findings expand the current understanding of
E. bieneusi
prevalence and genotype distribution in raccoon dogs in China. Our study also shows that raccoon dogs are hosts for
E. bieneusi
belonging to several genotypes, including zoonotic ones, highlighting the possibility of transmission of this pathogen between raccoon dogs and humans.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-020-06693-5</identifier><identifier>PMID: 32377907</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; China - epidemiology ; Disease transmission ; Dogs ; Enterocytozoon - genetics ; Enterocytozoon bieneusi ; Farms ; Feces - parasitology ; Female ; Genetic aspects ; Genotype ; Genotype & phenotype ; Genotypes ; Immunology ; Male ; Medical Microbiology ; Microbiology ; Microsporidiosis - epidemiology ; Microsporidiosis - parasitology ; Microsporidiosis - veterinary ; Nyctereutes procyonoides ; Phylogeny ; Polymerase Chain Reaction ; Prevalence ; Protozoology - Original Paper ; Raccoon Dogs - parasitology ; Ribosomal DNA ; Sequence analysis ; Statistical analysis</subject><ispartof>Parasitology research (1987), 2020-06, Vol.119 (6), p.1873-1878</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-6f4d10d42f6c248e2061ce4b1cfc035370e1d51c99b96f01049bd7e1ff57ef093</citedby><cites>FETCH-LOGICAL-c442t-6f4d10d42f6c248e2061ce4b1cfc035370e1d51c99b96f01049bd7e1ff57ef093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-020-06693-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-020-06693-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32377907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Yuan-Yuan</creatorcontrib><creatorcontrib>Ma, Ye-Ting</creatorcontrib><creatorcontrib>Nie, Lan-Bi</creatorcontrib><creatorcontrib>Li, Tao-Shan</creatorcontrib><creatorcontrib>Peng, Jun-Jie</creatorcontrib><creatorcontrib>Cong, Wei</creatorcontrib><creatorcontrib>Zou, Yang</creatorcontrib><creatorcontrib>Zhu, Xing-Quan</creatorcontrib><title>Prevalence and genotype distribution of Enterocytozoon bieneusi in farmed raccoon dogs (Nyctereutes procyonoides) in Shandong Province, eastern China</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Enterocytozoon bieneusi
is a common microsporidian species, which can infect humans and various species of animals. However, little is known about
E. bieneusi
prevalence and genotypes in farmed raccoon dogs (
Nyctereutes procyonoides
) in Shandong Province, China. In this study, a total of 356 fecal samples were collected from farmed raccoon dogs in Weihai, Weifang, and Yantai cities in Shandong Province, China. A total of 23 (6.5%) samples were
E. bieneusi
-positive by nested PCR amplification of the internal transcribed spacer (ITS) region of ribosomal DNA. Statistical analysis showed that
E. bieneusi
prevalence in male raccoon dogs was higher than that in female raccoon dogs, and the highest
E. bieneusi
prevalence was detected in adult raccoon dogs. Sequence analysis revealed four known
E. bieneusi
genotypes (D, type IV, CHG1, and Peru8), and type IV (11/23) was the predominant genotype. The genotypes type IV, Peru8, and CHG1 were reported in raccoon dogs for the first time in China. Phylogenetic analysis showed that three human-pathogenic genotypes (D, type IV, and Peru8) were clustered into group 1, and the CHG1 belonged to group 2. These findings expand the current understanding of
E. bieneusi
prevalence and genotype distribution in raccoon dogs in China. Our study also shows that raccoon dogs are hosts for
E. bieneusi
belonging to several genotypes, including zoonotic ones, highlighting the possibility of transmission of this pathogen between raccoon dogs and humans.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>China - epidemiology</subject><subject>Disease transmission</subject><subject>Dogs</subject><subject>Enterocytozoon - genetics</subject><subject>Enterocytozoon bieneusi</subject><subject>Farms</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Immunology</subject><subject>Male</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Microsporidiosis - epidemiology</subject><subject>Microsporidiosis - parasitology</subject><subject>Microsporidiosis - veterinary</subject><subject>Nyctereutes procyonoides</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Prevalence</subject><subject>Protozoology - Original Paper</subject><subject>Raccoon Dogs - parasitology</subject><subject>Ribosomal DNA</subject><subject>Sequence analysis</subject><subject>Statistical analysis</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UtuKFDEQDaK44-oP-CABX1aw18ql05PHZVgvsOiC-hy6k8pslplkTLoXxv_wf03vrFdE8pBQdc6pStUh5CmDUwbQvSoAUqgGODSglBZNe48smBS8Ybpt75MF6PoGxsQReVTKNQDrlJQPyZHgous0dAvy7TLjTb_BaJH20dE1xjTud0hdKGMOwzSGFGny9DyOmJPdj-lrqpEhYMSpBBoi9X3eoqO5t3ZOubQu9OT93lYCTiMWupuJKabgsLyYGR-vaq0U1_Qyp5tQa7-k2JeKj3R1FWL_mDzw_abgk7v7mHx-ff5p9ba5-PDm3ersorFS8rFRXjoGTnKvLJdL5KCYRTkw6y2IVnSAzLXMaj1o5YGB1IPrkHnfdujrdI7JyUG3dvhlwjKabSgWN5s-YpqK4ULrpWBKqQp9_hf0Ok051u4Ml9AtFdfwG2pdZ2pC9GmsY5lFzZnirVBieat1-g9UPQ63waaIPtT4HwR-INicSsnozS6HbZ_3hoGZvWAOXjDVC-bWC6atpGd3HU9D3c9Pyo_lV4A4AEpNxTXmX1_6j-x33iK_6g</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Ma, Yuan-Yuan</creator><creator>Ma, Ye-Ting</creator><creator>Nie, Lan-Bi</creator><creator>Li, Tao-Shan</creator><creator>Peng, Jun-Jie</creator><creator>Cong, Wei</creator><creator>Zou, Yang</creator><creator>Zhu, Xing-Quan</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200601</creationdate><title>Prevalence and genotype distribution of Enterocytozoon bieneusi in farmed raccoon dogs (Nyctereutes procyonoides) in Shandong Province, eastern China</title><author>Ma, Yuan-Yuan ; Ma, Ye-Ting ; Nie, Lan-Bi ; Li, Tao-Shan ; Peng, Jun-Jie ; Cong, Wei ; Zou, Yang ; Zhu, Xing-Quan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-6f4d10d42f6c248e2061ce4b1cfc035370e1d51c99b96f01049bd7e1ff57ef093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>China - epidemiology</topic><topic>Disease transmission</topic><topic>Dogs</topic><topic>Enterocytozoon - genetics</topic><topic>Enterocytozoon bieneusi</topic><topic>Farms</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Genetic aspects</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>Genotypes</topic><topic>Immunology</topic><topic>Male</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Microsporidiosis - epidemiology</topic><topic>Microsporidiosis - parasitology</topic><topic>Microsporidiosis - veterinary</topic><topic>Nyctereutes procyonoides</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Prevalence</topic><topic>Protozoology - Original Paper</topic><topic>Raccoon Dogs - parasitology</topic><topic>Ribosomal DNA</topic><topic>Sequence analysis</topic><topic>Statistical analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Yuan-Yuan</creatorcontrib><creatorcontrib>Ma, Ye-Ting</creatorcontrib><creatorcontrib>Nie, Lan-Bi</creatorcontrib><creatorcontrib>Li, Tao-Shan</creatorcontrib><creatorcontrib>Peng, Jun-Jie</creatorcontrib><creatorcontrib>Cong, Wei</creatorcontrib><creatorcontrib>Zou, Yang</creatorcontrib><creatorcontrib>Zhu, Xing-Quan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Yuan-Yuan</au><au>Ma, Ye-Ting</au><au>Nie, Lan-Bi</au><au>Li, Tao-Shan</au><au>Peng, Jun-Jie</au><au>Cong, Wei</au><au>Zou, Yang</au><au>Zhu, Xing-Quan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and genotype distribution of Enterocytozoon bieneusi in farmed raccoon dogs (Nyctereutes procyonoides) in Shandong Province, eastern China</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>119</volume><issue>6</issue><spage>1873</spage><epage>1878</epage><pages>1873-1878</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Enterocytozoon bieneusi
is a common microsporidian species, which can infect humans and various species of animals. However, little is known about
E. bieneusi
prevalence and genotypes in farmed raccoon dogs (
Nyctereutes procyonoides
) in Shandong Province, China. In this study, a total of 356 fecal samples were collected from farmed raccoon dogs in Weihai, Weifang, and Yantai cities in Shandong Province, China. A total of 23 (6.5%) samples were
E. bieneusi
-positive by nested PCR amplification of the internal transcribed spacer (ITS) region of ribosomal DNA. Statistical analysis showed that
E. bieneusi
prevalence in male raccoon dogs was higher than that in female raccoon dogs, and the highest
E. bieneusi
prevalence was detected in adult raccoon dogs. Sequence analysis revealed four known
E. bieneusi
genotypes (D, type IV, CHG1, and Peru8), and type IV (11/23) was the predominant genotype. The genotypes type IV, Peru8, and CHG1 were reported in raccoon dogs for the first time in China. Phylogenetic analysis showed that three human-pathogenic genotypes (D, type IV, and Peru8) were clustered into group 1, and the CHG1 belonged to group 2. These findings expand the current understanding of
E. bieneusi
prevalence and genotype distribution in raccoon dogs in China. Our study also shows that raccoon dogs are hosts for
E. bieneusi
belonging to several genotypes, including zoonotic ones, highlighting the possibility of transmission of this pathogen between raccoon dogs and humans.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32377907</pmid><doi>10.1007/s00436-020-06693-5</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Parasitology research (1987), 2020-06, Vol.119 (6), p.1873-1878 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2399831666 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biomedical and Life Sciences Biomedicine China - epidemiology Disease transmission Dogs Enterocytozoon - genetics Enterocytozoon bieneusi Farms Feces - parasitology Female Genetic aspects Genotype Genotype & phenotype Genotypes Immunology Male Medical Microbiology Microbiology Microsporidiosis - epidemiology Microsporidiosis - parasitology Microsporidiosis - veterinary Nyctereutes procyonoides Phylogeny Polymerase Chain Reaction Prevalence Protozoology - Original Paper Raccoon Dogs - parasitology Ribosomal DNA Sequence analysis Statistical analysis |
| title | Prevalence and genotype distribution of Enterocytozoon bieneusi in farmed raccoon dogs (Nyctereutes procyonoides) in Shandong Province, eastern China |
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