Targeted DNA complementation on a 1,1′-carbonyldiimidazole-functionalized surface for identifying Mycobacterium tuberculosis
Herein, a rapid and sensitive current–volt measurement was developed for identifying the IS6110 DNA sequence to diagnose Mycobacterium tuberculosis (TB). An aminated capture probe was immobilized on a 1,1′-carbonyldiimidazole-functionalized interdigitated electrode (IDE) silica substrate, and the ta...
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| Veröffentlicht in: | 3 Biotech 2020-05, Vol.10 (5), p.227-227, Article 227 |
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| creator | Xu, Shu Xue, Yu Guo, Fengyan Xu, Miaomiao Gopinath, Subash C. B. Mao, Xiaohui |
| description | Herein, a rapid and sensitive current–volt measurement was developed for identifying the IS6110 DNA sequence to diagnose
Mycobacterium tuberculosis
(TB). An aminated capture probe was immobilized on a 1,1′-carbonyldiimidazole-functionalized interdigitated electrode (IDE) silica substrate, and the target sequence was detected by complementation. It was found that all tested concentrations displayed a higher response in current changes than the control, and the limit of detection was 10 fM. The sensitivity ranged from 1 to 10 fM. The control sequences with single-, triple-mismatch and noncomplementary sequences showed great discrimination. This rapid and easy DNA detection method helps to identify
M. tuberculosis
for early-stage diagnosis of TB. |
| doi_str_mv | 10.1007/s13205-020-02216-2 |
| format | Article |
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Mycobacterium tuberculosis
(TB). An aminated capture probe was immobilized on a 1,1′-carbonyldiimidazole-functionalized interdigitated electrode (IDE) silica substrate, and the target sequence was detected by complementation. It was found that all tested concentrations displayed a higher response in current changes than the control, and the limit of detection was 10 fM. The sensitivity ranged from 1 to 10 fM. The control sequences with single-, triple-mismatch and noncomplementary sequences showed great discrimination. This rapid and easy DNA detection method helps to identify
M. tuberculosis
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Mycobacterium tuberculosis
(TB). An aminated capture probe was immobilized on a 1,1′-carbonyldiimidazole-functionalized interdigitated electrode (IDE) silica substrate, and the target sequence was detected by complementation. It was found that all tested concentrations displayed a higher response in current changes than the control, and the limit of detection was 10 fM. The sensitivity ranged from 1 to 10 fM. The control sequences with single-, triple-mismatch and noncomplementary sequences showed great discrimination. This rapid and easy DNA detection method helps to identify
M. tuberculosis
for early-stage diagnosis of TB.</description><subject>Agriculture</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Biotechnology & Applied Microbiology</subject><subject>Cancer Research</subject><subject>Carbonyls</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Life Sciences & Biomedicine</subject><subject>Mycobacterium tuberculosis</subject><subject>Nucleotide sequence</subject><subject>Original</subject><subject>Original Article</subject><subject>Science & Technology</subject><subject>Silica</subject><subject>Silicon dioxide</subject><subject>Stem Cells</subject><subject>Substrates</subject><subject>Target detection</subject><subject>Tuberculosis</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><recordid>eNqNks2KFDEQxxtR3GXdF_AgDV6EtTWVdE86F2EZP2HVywreQjqpHrN0J2PSUWYP4jP5SD6J6e11_DiIISGB_OpPVf2rKO4CeQSE8McRGCVNRSjJh8KqojeKQwqCVA1n7c39m74_KI5jvCB5NdAIILeLA0YZZzWIw-LLuQobnNCUT9-cltqP2wFHdJOarHdl3qqEh_D967dKq9B5txuMtaM16tIPWPXJ6RlUg73MEjGFXmksex9Ka7KK7XfWbcrXO-07pScMNo3llDoMOg0-2ninuNWrIeLx9X1UvHv-7Hz9sjp7--LV-vSs0jVtpoohIx0VNetrjkwBY127YiAEaUXLlSFCG6EYIKfaUNNwUyMXhnUALdakY0fFk0V3m7oRjc65BTXIbbCjCjvplZV__jj7QW78J8lBrACaLPDgWiD4jwnjJEcbNQ6DcuhTlJQJkZtKGpLR-3-hFz6F3KMriue0W6CZogulg48xYL9PBoicHZaLwzI7LK8clnPQvd_L2If89DMDJwvwGTvfR23Radxj8wgwaFe8nacBMt3-P722y1CsfXJTDmVLaMy422D4VeQ_8v8BygzVDw</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Xu, Shu</creator><creator>Xue, Yu</creator><creator>Guo, Fengyan</creator><creator>Xu, Miaomiao</creator><creator>Gopinath, Subash C. 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Mycobacterium tuberculosis
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M. tuberculosis
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| subjects | Agriculture Bioinformatics Biomaterials Biotechnology Biotechnology & Applied Microbiology Cancer Research Carbonyls Chemistry Chemistry and Materials Science Deoxyribonucleic acid DNA Life Sciences & Biomedicine Mycobacterium tuberculosis Nucleotide sequence Original Original Article Science & Technology Silica Silicon dioxide Stem Cells Substrates Target detection Tuberculosis |
| title | Targeted DNA complementation on a 1,1′-carbonyldiimidazole-functionalized surface for identifying Mycobacterium tuberculosis |
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