Molecular characterization, expression pattern and RNAi‐mediated silencing of vitellogenin receptor gene in almond moth, Cadra cautella
The almond moth is an important pest of date fruits worldwide. The females produce several eggs; however, role of vitellogenin receptor (VgR) in oocyte development by mediating endocytosis of major yolk protein precursor Vg remains yet unexplored. To investigate the role of vitellogenin receptor (Vg...
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| Veröffentlicht in: | Insect molecular biology 2020-08, Vol.29 (4), p.417-430 |
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| creator | Husain, M. Rasool, K. G. Tufail, M. Aldawood, A. S. |
| description | The almond moth is an important pest of date fruits worldwide. The females produce several eggs; however, role of vitellogenin receptor (VgR) in oocyte development by mediating endocytosis of major yolk protein precursor Vg remains yet unexplored. To investigate the role of vitellogenin receptor (VgR) in reproduction, Cadra cautella vitellogenin receptor (CcVgR) transcript was obtained using rapid amplification of cDNA ends‐polymerase chain reaction. Expression analysis of CcVgR was performed using reverse transcriptase and quantitative polymerase chain reaction (qPCR) in different developmental stages. RNA interference was performed by injecting CcVgR‐based double‐stranded (ds)RNA at different exposure times. The results revealed that CcVgR is 5421 bp long, encoded 1807 amino acid, belongs to low‐density lipoprotein receptor superfamily and contains all conserved domains. Expression analysis confirmed that CcVgR is sex‐specific and starts to express in female larvae on day 19. Additionally, RNA interference (RNAi) of CcVgR‐based dsRNA inhibited CcVgR expression up to 83% after 72 h, reduced fecundity and hatchability, and confirmed involvement of CcVgR in C. cautella reproduction. This report provides a basis for gene silencing in this species, and proposes RNAi technology potential for pest management. |
| doi_str_mv | 10.1111/imb.12646 |
| format | Article |
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G. ; Tufail, M. ; Aldawood, A. S.</creator><creatorcontrib>Husain, M. ; Rasool, K. G. ; Tufail, M. ; Aldawood, A. S.</creatorcontrib><description>The almond moth is an important pest of date fruits worldwide. The females produce several eggs; however, role of vitellogenin receptor (VgR) in oocyte development by mediating endocytosis of major yolk protein precursor Vg remains yet unexplored. To investigate the role of vitellogenin receptor (VgR) in reproduction, Cadra cautella vitellogenin receptor (CcVgR) transcript was obtained using rapid amplification of cDNA ends‐polymerase chain reaction. Expression analysis of CcVgR was performed using reverse transcriptase and quantitative polymerase chain reaction (qPCR) in different developmental stages. RNA interference was performed by injecting CcVgR‐based double‐stranded (ds)RNA at different exposure times. The results revealed that CcVgR is 5421 bp long, encoded 1807 amino acid, belongs to low‐density lipoprotein receptor superfamily and contains all conserved domains. Expression analysis confirmed that CcVgR is sex‐specific and starts to express in female larvae on day 19. Additionally, RNA interference (RNAi) of CcVgR‐based dsRNA inhibited CcVgR expression up to 83% after 72 h, reduced fecundity and hatchability, and confirmed involvement of CcVgR in C. cautella reproduction. This report provides a basis for gene silencing in this species, and proposes RNAi technology potential for pest management.</description><identifier>ISSN: 0962-1075</identifier><identifier>EISSN: 1365-2583</identifier><identifier>DOI: 10.1111/imb.12646</identifier><identifier>PMID: 32368832</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Amino Acid Sequence ; Amino acids ; Animals ; Cadra cautella ; Complementary DNA ; dates ; Developmental stages ; Double-stranded RNA ; Egg Proteins - chemistry ; Egg Proteins - genetics ; Egg Proteins - metabolism ; Eggs ; Endocytosis ; expression ; Fecundity ; Female ; Gametocytes ; Gene Expression ; Gene Expression Profiling ; Gene silencing ; Hatchability ; Insect Proteins - chemistry ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Interference ; Larva - growth & development ; Larva - metabolism ; Larvae ; Male ; Moths - genetics ; Moths - growth & development ; Moths - metabolism ; Pest control ; Pests ; Phylogeny ; Polymerase chain reaction ; Pupa - growth & development ; Pupa - metabolism ; Receptor density ; Receptors ; Receptors, Cell Surface - chemistry ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; Reproduction ; Reproduction (biology) ; Ribonucleic acid ; RNA ; RNA Interference ; RNA-directed DNA polymerase ; RNA-mediated interference ; Sequence Alignment ; Transcription ; Vitellogenin ; vitellogenin receptor ; Yolk ; Yolk protein</subject><ispartof>Insect molecular biology, 2020-08, Vol.29 (4), p.417-430</ispartof><rights>2020 The Royal Entomological Society</rights><rights>2020 The Royal Entomological Society.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3136-7089f727fe5db0d8c4d60f4308f6820b49af13dce110d4e28e97d95344ef880e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fimb.12646$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fimb.12646$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32368832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Husain, M.</creatorcontrib><creatorcontrib>Rasool, K. G.</creatorcontrib><creatorcontrib>Tufail, M.</creatorcontrib><creatorcontrib>Aldawood, A. S.</creatorcontrib><title>Molecular characterization, expression pattern and RNAi‐mediated silencing of vitellogenin receptor gene in almond moth, Cadra cautella</title><title>Insect molecular biology</title><addtitle>Insect Mol Biol</addtitle><description>The almond moth is an important pest of date fruits worldwide. The females produce several eggs; however, role of vitellogenin receptor (VgR) in oocyte development by mediating endocytosis of major yolk protein precursor Vg remains yet unexplored. To investigate the role of vitellogenin receptor (VgR) in reproduction, Cadra cautella vitellogenin receptor (CcVgR) transcript was obtained using rapid amplification of cDNA ends‐polymerase chain reaction. Expression analysis of CcVgR was performed using reverse transcriptase and quantitative polymerase chain reaction (qPCR) in different developmental stages. RNA interference was performed by injecting CcVgR‐based double‐stranded (ds)RNA at different exposure times. The results revealed that CcVgR is 5421 bp long, encoded 1807 amino acid, belongs to low‐density lipoprotein receptor superfamily and contains all conserved domains. Expression analysis confirmed that CcVgR is sex‐specific and starts to express in female larvae on day 19. Additionally, RNA interference (RNAi) of CcVgR‐based dsRNA inhibited CcVgR expression up to 83% after 72 h, reduced fecundity and hatchability, and confirmed involvement of CcVgR in C. cautella reproduction. This report provides a basis for gene silencing in this species, and proposes RNAi technology potential for pest management.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Cadra cautella</subject><subject>Complementary DNA</subject><subject>dates</subject><subject>Developmental stages</subject><subject>Double-stranded RNA</subject><subject>Egg Proteins - chemistry</subject><subject>Egg Proteins - genetics</subject><subject>Egg Proteins - metabolism</subject><subject>Eggs</subject><subject>Endocytosis</subject><subject>expression</subject><subject>Fecundity</subject><subject>Female</subject><subject>Gametocytes</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Gene silencing</subject><subject>Hatchability</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Interference</subject><subject>Larva - growth & development</subject><subject>Larva - metabolism</subject><subject>Larvae</subject><subject>Male</subject><subject>Moths - genetics</subject><subject>Moths - growth & development</subject><subject>Moths - metabolism</subject><subject>Pest control</subject><subject>Pests</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Pupa - growth & development</subject><subject>Pupa - metabolism</subject><subject>Receptor density</subject><subject>Receptors</subject><subject>Receptors, Cell Surface - chemistry</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Reproduction</subject><subject>Reproduction (biology)</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA-directed DNA polymerase</subject><subject>RNA-mediated interference</subject><subject>Sequence Alignment</subject><subject>Transcription</subject><subject>Vitellogenin</subject><subject>vitellogenin receptor</subject><subject>Yolk</subject><subject>Yolk protein</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kbtuFDEUhi0EIstCwQsgSzQgZRLfxmOXyYpLpAQkBLXltY8TRzPjwZ4BQkVLxzPyJHjZQIGEG9--8-uc_0foMSVHtK7jOGyPKJNC3kErymXbsFbxu2hFtGQNJV17gB6Uck0IUVrq--iAMy6V4myFvl-kHtzS24zdlc3WzZDjVzvHNB5i-DJlKKWe8WTn-jNiO3r87s1J_PntxwA-2hk8LrGH0cXxEqeAP8UZ-j5dwhhHnMHBNKeM6xVwfbD9kKrCkOarQ7yxPlvs7LKrsA_RvWD7Ao9u9zX68PLF-83r5vztq7PNyXnjeJ2t6eoMoWNdgNZviVdOeEmC4EQFqRjZCm0D5d4BpcQLYAp053XLhYCgFAG-Rs_2ulNOHxcosxlicbsORkhLMYxrJakWTFf06T_odVryWLszTFDdVWer8ho931Mup1IyBDPlONh8Yygxu3xMzcf8zqeyT24Vl2317y_5J5AKHO-Bz9XUm_8rmbOL073kL3vZnA0</recordid><startdate>202008</startdate><enddate>202008</enddate><creator>Husain, M.</creator><creator>Rasool, K. G.</creator><creator>Tufail, M.</creator><creator>Aldawood, A. S.</creator><general>John Wiley & Sons, Ltd</general><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>202008</creationdate><title>Molecular characterization, expression pattern and RNAi‐mediated silencing of vitellogenin receptor gene in almond moth, Cadra cautella</title><author>Husain, M. ; Rasool, K. G. ; Tufail, M. ; Aldawood, A. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3136-7089f727fe5db0d8c4d60f4308f6820b49af13dce110d4e28e97d95344ef880e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Cadra cautella</topic><topic>Complementary DNA</topic><topic>dates</topic><topic>Developmental stages</topic><topic>Double-stranded RNA</topic><topic>Egg Proteins - chemistry</topic><topic>Egg Proteins - genetics</topic><topic>Egg Proteins - metabolism</topic><topic>Eggs</topic><topic>Endocytosis</topic><topic>expression</topic><topic>Fecundity</topic><topic>Female</topic><topic>Gametocytes</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Gene silencing</topic><topic>Hatchability</topic><topic>Insect Proteins - chemistry</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Interference</topic><topic>Larva - growth & development</topic><topic>Larva - metabolism</topic><topic>Larvae</topic><topic>Male</topic><topic>Moths - genetics</topic><topic>Moths - growth & development</topic><topic>Moths - metabolism</topic><topic>Pest control</topic><topic>Pests</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Pupa - growth & development</topic><topic>Pupa - metabolism</topic><topic>Receptor density</topic><topic>Receptors</topic><topic>Receptors, Cell Surface - chemistry</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Reproduction</topic><topic>Reproduction (biology)</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>RNA-directed DNA polymerase</topic><topic>RNA-mediated interference</topic><topic>Sequence Alignment</topic><topic>Transcription</topic><topic>Vitellogenin</topic><topic>vitellogenin receptor</topic><topic>Yolk</topic><topic>Yolk protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Husain, M.</creatorcontrib><creatorcontrib>Rasool, K. G.</creatorcontrib><creatorcontrib>Tufail, M.</creatorcontrib><creatorcontrib>Aldawood, A. S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Husain, M.</au><au>Rasool, K. G.</au><au>Tufail, M.</au><au>Aldawood, A. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization, expression pattern and RNAi‐mediated silencing of vitellogenin receptor gene in almond moth, Cadra cautella</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>2020-08</date><risdate>2020</risdate><volume>29</volume><issue>4</issue><spage>417</spage><epage>430</epage><pages>417-430</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>The almond moth is an important pest of date fruits worldwide. The females produce several eggs; however, role of vitellogenin receptor (VgR) in oocyte development by mediating endocytosis of major yolk protein precursor Vg remains yet unexplored. To investigate the role of vitellogenin receptor (VgR) in reproduction, Cadra cautella vitellogenin receptor (CcVgR) transcript was obtained using rapid amplification of cDNA ends‐polymerase chain reaction. Expression analysis of CcVgR was performed using reverse transcriptase and quantitative polymerase chain reaction (qPCR) in different developmental stages. RNA interference was performed by injecting CcVgR‐based double‐stranded (ds)RNA at different exposure times. The results revealed that CcVgR is 5421 bp long, encoded 1807 amino acid, belongs to low‐density lipoprotein receptor superfamily and contains all conserved domains. Expression analysis confirmed that CcVgR is sex‐specific and starts to express in female larvae on day 19. Additionally, RNA interference (RNAi) of CcVgR‐based dsRNA inhibited CcVgR expression up to 83% after 72 h, reduced fecundity and hatchability, and confirmed involvement of CcVgR in C. cautella reproduction. This report provides a basis for gene silencing in this species, and proposes RNAi technology potential for pest management.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>32368832</pmid><doi>10.1111/imb.12646</doi><tpages>14</tpages></addata></record> |
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| ispartof | Insect molecular biology, 2020-08, Vol.29 (4), p.417-430 |
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| subjects | Amino Acid Sequence Amino acids Animals Cadra cautella Complementary DNA dates Developmental stages Double-stranded RNA Egg Proteins - chemistry Egg Proteins - genetics Egg Proteins - metabolism Eggs Endocytosis expression Fecundity Female Gametocytes Gene Expression Gene Expression Profiling Gene silencing Hatchability Insect Proteins - chemistry Insect Proteins - genetics Insect Proteins - metabolism Interference Larva - growth & development Larva - metabolism Larvae Male Moths - genetics Moths - growth & development Moths - metabolism Pest control Pests Phylogeny Polymerase chain reaction Pupa - growth & development Pupa - metabolism Receptor density Receptors Receptors, Cell Surface - chemistry Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism Reproduction Reproduction (biology) Ribonucleic acid RNA RNA Interference RNA-directed DNA polymerase RNA-mediated interference Sequence Alignment Transcription Vitellogenin vitellogenin receptor Yolk Yolk protein |
| title | Molecular characterization, expression pattern and RNAi‐mediated silencing of vitellogenin receptor gene in almond moth, Cadra cautella |
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