The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification
Ice-binding proteins (IBPs) facilitate organism survival under extreme conditions by inhibiting thermal hysteresis and ice recrystallization. IBPs have been widely used as cryoprotectants to cryopreserve mammalian gametes and embryos. In the present study, we evaluated the protective effects of an A...
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| Veröffentlicht in: | Theriogenology 2020-07, Vol.151, p.137-143 |
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| creator | Sun, Wu-Sheng Jang, Hoon Kwon, Hyo Jin Kim, Ki Young Ahn, Soo Bin Hwang, Seongsoo Lee, Sung Gu Lee, Jun Hyuck Hwang, In-Sul Lee, Jeong-Woong |
| description | Ice-binding proteins (IBPs) facilitate organism survival under extreme conditions by inhibiting thermal hysteresis and ice recrystallization. IBPs have been widely used as cryoprotectants to cryopreserve mammalian gametes and embryos. In the present study, we evaluated the protective effects of an Arctic yeast, Leucosporidium sp. AY30 derived ice-binding protein (LeIBP), on the vitrification of bovine metaphase II (MII) oocytes and embryos. When oocytes and embryos were frozen using the two-step vitrification method, the survival rate was significantly increased in the presence of LeIBP. The LeIBP supplementation decreased the levels of intracellular reactive oxygen species (ROS) and enhanced mitochondrial functions in the vitrified–warmed oocytes. Furthermore, LeIBP improved the developmental potential and suppressed apoptosis of the embryos derived from vitrified–warmed oocytes. Collectively, these data indicate that LeIBP can be used as a promising cryoprotectant to prevent cryoinjury during vitrification in bovine oocytes.
•LeIBP enhanced the viability, developmental rate of the vitrified bovine oocytes and embryos.•LeIBP decreased the oxidative stress during vitrification.•LeIBP can be used as a cryoprotectant during bovine oocyte vitrification. |
| doi_str_mv | 10.1016/j.theriogenology.2020.04.016 |
| format | Article |
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•LeIBP enhanced the viability, developmental rate of the vitrified bovine oocytes and embryos.•LeIBP decreased the oxidative stress during vitrification.•LeIBP can be used as a cryoprotectant during bovine oocyte vitrification.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2020.04.016</identifier><identifier>PMID: 32361180</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Basidiomycota - metabolism ; Bovine oocyte ; Cattle ; Cryopreservation - veterinary ; Cryoprotective Agents - chemistry ; Cryoprotective Agents - metabolism ; Cryoprotective Agents - pharmacology ; Embryo development ; Embryo, Mammalian ; Fungal Proteins - metabolism ; Fungal Proteins - pharmacology ; LeIBP ; Oocytes - drug effects ; Vitrification ; Vitrification - drug effects</subject><ispartof>Theriogenology, 2020-07, Vol.151, p.137-143</ispartof><rights>2021</rights><rights>Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-735bb16b62e07b99edb8a029a72dca21f25474e4b9685f8aaf8bc5d2b64baedd3</citedby><cites>FETCH-LOGICAL-c386t-735bb16b62e07b99edb8a029a72dca21f25474e4b9685f8aaf8bc5d2b64baedd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0093691X20302363$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32361180$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Wu-Sheng</creatorcontrib><creatorcontrib>Jang, Hoon</creatorcontrib><creatorcontrib>Kwon, Hyo Jin</creatorcontrib><creatorcontrib>Kim, Ki Young</creatorcontrib><creatorcontrib>Ahn, Soo Bin</creatorcontrib><creatorcontrib>Hwang, Seongsoo</creatorcontrib><creatorcontrib>Lee, Sung Gu</creatorcontrib><creatorcontrib>Lee, Jun Hyuck</creatorcontrib><creatorcontrib>Hwang, In-Sul</creatorcontrib><creatorcontrib>Lee, Jeong-Woong</creatorcontrib><title>The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>Ice-binding proteins (IBPs) facilitate organism survival under extreme conditions by inhibiting thermal hysteresis and ice recrystallization. IBPs have been widely used as cryoprotectants to cryopreserve mammalian gametes and embryos. In the present study, we evaluated the protective effects of an Arctic yeast, Leucosporidium sp. AY30 derived ice-binding protein (LeIBP), on the vitrification of bovine metaphase II (MII) oocytes and embryos. When oocytes and embryos were frozen using the two-step vitrification method, the survival rate was significantly increased in the presence of LeIBP. The LeIBP supplementation decreased the levels of intracellular reactive oxygen species (ROS) and enhanced mitochondrial functions in the vitrified–warmed oocytes. Furthermore, LeIBP improved the developmental potential and suppressed apoptosis of the embryos derived from vitrified–warmed oocytes. Collectively, these data indicate that LeIBP can be used as a promising cryoprotectant to prevent cryoinjury during vitrification in bovine oocytes.
•LeIBP enhanced the viability, developmental rate of the vitrified bovine oocytes and embryos.•LeIBP decreased the oxidative stress during vitrification.•LeIBP can be used as a cryoprotectant during bovine oocyte vitrification.</description><subject>Animals</subject><subject>Basidiomycota - metabolism</subject><subject>Bovine oocyte</subject><subject>Cattle</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotective Agents - chemistry</subject><subject>Cryoprotective Agents - metabolism</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Embryo development</subject><subject>Embryo, Mammalian</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungal Proteins - pharmacology</subject><subject>LeIBP</subject><subject>Oocytes - drug effects</subject><subject>Vitrification</subject><subject>Vitrification - drug effects</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU1v1DAQtRCIbgt_AfnAoRwSbCdxEokLVLRUWokeWomb5Y_JdlYbe7GTSHvpb8erLUi9cZqR3nvzZuYR8pGzkjMuP2_L6REihg34sAubQymYYCWrywy-IivetX1RiYq_JivG-qqQPf91Rs5T2jLGKin5W3KWccl5x1bk6f4R6D6GCeyEC1AYhtzRMNA1zDakfYjocB4Llz0XcBQtFAa9Q7856dDTyzXcfrv7RIOnJizogYZgDxMkqr2jMJp4CIm6OR5FC04RB7R6wuDfkTeD3iV4_1wvyMP19_urH8X6583t1dd1YatOTkVbNcZwaaQA1pq-B2c6zUSvW-GsFnwQTd3WUJteds3QaT10xjZOGFkbDc5VF-TyNDev_HuGNKkRk4XdTnsIc1Ki6jve9LzmmfrlRLUxpBRhUPuIo44HxZk6JqC26mUC6piAYrXKYJZ_eHaazQjun_jvyzPh-kSAfO-CEFWyCN6Cw5hfr1zA_3P6A3ONo8g</recordid><startdate>20200715</startdate><enddate>20200715</enddate><creator>Sun, Wu-Sheng</creator><creator>Jang, Hoon</creator><creator>Kwon, Hyo Jin</creator><creator>Kim, Ki Young</creator><creator>Ahn, Soo Bin</creator><creator>Hwang, Seongsoo</creator><creator>Lee, Sung Gu</creator><creator>Lee, Jun Hyuck</creator><creator>Hwang, In-Sul</creator><creator>Lee, Jeong-Woong</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200715</creationdate><title>The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification</title><author>Sun, Wu-Sheng ; Jang, Hoon ; Kwon, Hyo Jin ; Kim, Ki Young ; Ahn, Soo Bin ; Hwang, Seongsoo ; Lee, Sung Gu ; Lee, Jun Hyuck ; Hwang, In-Sul ; Lee, Jeong-Woong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-735bb16b62e07b99edb8a029a72dca21f25474e4b9685f8aaf8bc5d2b64baedd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Basidiomycota - metabolism</topic><topic>Bovine oocyte</topic><topic>Cattle</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotective Agents - chemistry</topic><topic>Cryoprotective Agents - metabolism</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Embryo development</topic><topic>Embryo, Mammalian</topic><topic>Fungal Proteins - metabolism</topic><topic>Fungal Proteins - pharmacology</topic><topic>LeIBP</topic><topic>Oocytes - drug effects</topic><topic>Vitrification</topic><topic>Vitrification - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Wu-Sheng</creatorcontrib><creatorcontrib>Jang, Hoon</creatorcontrib><creatorcontrib>Kwon, Hyo Jin</creatorcontrib><creatorcontrib>Kim, Ki Young</creatorcontrib><creatorcontrib>Ahn, Soo Bin</creatorcontrib><creatorcontrib>Hwang, Seongsoo</creatorcontrib><creatorcontrib>Lee, Sung Gu</creatorcontrib><creatorcontrib>Lee, Jun Hyuck</creatorcontrib><creatorcontrib>Hwang, In-Sul</creatorcontrib><creatorcontrib>Lee, Jeong-Woong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Wu-Sheng</au><au>Jang, Hoon</au><au>Kwon, Hyo Jin</au><au>Kim, Ki Young</au><au>Ahn, Soo Bin</au><au>Hwang, Seongsoo</au><au>Lee, Sung Gu</au><au>Lee, Jun Hyuck</au><au>Hwang, In-Sul</au><au>Lee, Jeong-Woong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2020-07-15</date><risdate>2020</risdate><volume>151</volume><spage>137</spage><epage>143</epage><pages>137-143</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>Ice-binding proteins (IBPs) facilitate organism survival under extreme conditions by inhibiting thermal hysteresis and ice recrystallization. IBPs have been widely used as cryoprotectants to cryopreserve mammalian gametes and embryos. In the present study, we evaluated the protective effects of an Arctic yeast, Leucosporidium sp. AY30 derived ice-binding protein (LeIBP), on the vitrification of bovine metaphase II (MII) oocytes and embryos. When oocytes and embryos were frozen using the two-step vitrification method, the survival rate was significantly increased in the presence of LeIBP. The LeIBP supplementation decreased the levels of intracellular reactive oxygen species (ROS) and enhanced mitochondrial functions in the vitrified–warmed oocytes. Furthermore, LeIBP improved the developmental potential and suppressed apoptosis of the embryos derived from vitrified–warmed oocytes. Collectively, these data indicate that LeIBP can be used as a promising cryoprotectant to prevent cryoinjury during vitrification in bovine oocytes.
•LeIBP enhanced the viability, developmental rate of the vitrified bovine oocytes and embryos.•LeIBP decreased the oxidative stress during vitrification.•LeIBP can be used as a cryoprotectant during bovine oocyte vitrification.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>32361180</pmid><doi>10.1016/j.theriogenology.2020.04.016</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Basidiomycota - metabolism Bovine oocyte Cattle Cryopreservation - veterinary Cryoprotective Agents - chemistry Cryoprotective Agents - metabolism Cryoprotective Agents - pharmacology Embryo development Embryo, Mammalian Fungal Proteins - metabolism Fungal Proteins - pharmacology LeIBP Oocytes - drug effects Vitrification Vitrification - drug effects |
| title | The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification |
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