G-quadruplex-based assay combined with aptamer and gold nanoparticles for Escherichia coli K88 determination
A colorimetric method was developed using G-quadruplex and gold nanoparticles (AuNPs) for determination of Escherichia coli K88 ( ETEC K88 ). It was composed of two modules: (1) an aptamer as biorecognizing element and (2) a capturing DNA (modified with AuNPs at 5′) as a transducer. In the absence o...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2020-05, Vol.187 (5), p.308-308, Article 308 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Wang, Zefeng Lu, Qiujun Xu, Tao Wang, Feiying Huang, Fangfang Peng, Yanling Deng, Le |
| description | A colorimetric method was developed using G-quadruplex and gold nanoparticles (AuNPs) for determination of
Escherichia coli K88
(
ETEC K88
). It was composed of two modules: (1) an aptamer as biorecognizing element and (2) a capturing DNA (modified with AuNPs at 5′) as a transducer. In the absence of target bacteria, the aptamer can form stable double strands with capturing DNA, preventing the binding of capturing DNA to the G-quadruplex. However, the double strands of capturing DNA and aptamer are untied due to the stronger binding of aptamers to bacteria in the presence of target bacteria. As a result, the G-quadruplex binds to capture DNA and leads to the aggregation and color change of AuNPs, which can be monitored by a spectrophotometer or visualization. The quantitative determination was achieved by monitoring the optical density change of AuNPs solution at 524 nm after target addition. Under optimal conditions, the method has a low detection limit (1.35 × 10
2
CFU mL
−1
) and a linear response in the range 10
2
to 10
6
CFU mL
−1
.
Graphical abstract
The manuscripts describe a colorimetric method for the detection of
ETEC K88
by using intermolecular G-quadruplex to induce the agglomeration of gold nanoparticles, which can be directly used to determine the presence of bacteria with our naked eyes. |
| doi_str_mv | 10.1007/s00604-020-04291-x |
| format | Article |
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Escherichia coli K88
(
ETEC K88
). It was composed of two modules: (1) an aptamer as biorecognizing element and (2) a capturing DNA (modified with AuNPs at 5′) as a transducer. In the absence of target bacteria, the aptamer can form stable double strands with capturing DNA, preventing the binding of capturing DNA to the G-quadruplex. However, the double strands of capturing DNA and aptamer are untied due to the stronger binding of aptamers to bacteria in the presence of target bacteria. As a result, the G-quadruplex binds to capture DNA and leads to the aggregation and color change of AuNPs, which can be monitored by a spectrophotometer or visualization. The quantitative determination was achieved by monitoring the optical density change of AuNPs solution at 524 nm after target addition. Under optimal conditions, the method has a low detection limit (1.35 × 10
2
CFU mL
−1
) and a linear response in the range 10
2
to 10
6
CFU mL
−1
.
Graphical abstract
The manuscripts describe a colorimetric method for the detection of
ETEC K88
by using intermolecular G-quadruplex to induce the agglomeration of gold nanoparticles, which can be directly used to determine the presence of bacteria with our naked eyes.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-020-04291-x</identifier><identifier>PMID: 32356133</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Bacteria ; Binding ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Colorimetry ; Deoxyribonucleic acid ; DNA ; E coli ; Escherichia coli ; Gold ; Microengineering ; Nanochemistry ; Nanoparticles ; Nanotechnology ; Optical density ; Original Paper ; Strands</subject><ispartof>Mikrochimica acta (1966), 2020-05, Vol.187 (5), p.308-308, Article 308</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-8c54af0163e274d4052c995270e341c21378310d0689a2105527c251d21c6fab3</citedby><cites>FETCH-LOGICAL-c414t-8c54af0163e274d4052c995270e341c21378310d0689a2105527c251d21c6fab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-020-04291-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-020-04291-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32356133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Zefeng</creatorcontrib><creatorcontrib>Lu, Qiujun</creatorcontrib><creatorcontrib>Xu, Tao</creatorcontrib><creatorcontrib>Wang, Feiying</creatorcontrib><creatorcontrib>Huang, Fangfang</creatorcontrib><creatorcontrib>Peng, Yanling</creatorcontrib><creatorcontrib>Deng, Le</creatorcontrib><title>G-quadruplex-based assay combined with aptamer and gold nanoparticles for Escherichia coli K88 determination</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>A colorimetric method was developed using G-quadruplex and gold nanoparticles (AuNPs) for determination of
Escherichia coli K88
(
ETEC K88
). It was composed of two modules: (1) an aptamer as biorecognizing element and (2) a capturing DNA (modified with AuNPs at 5′) as a transducer. In the absence of target bacteria, the aptamer can form stable double strands with capturing DNA, preventing the binding of capturing DNA to the G-quadruplex. However, the double strands of capturing DNA and aptamer are untied due to the stronger binding of aptamers to bacteria in the presence of target bacteria. As a result, the G-quadruplex binds to capture DNA and leads to the aggregation and color change of AuNPs, which can be monitored by a spectrophotometer or visualization. The quantitative determination was achieved by monitoring the optical density change of AuNPs solution at 524 nm after target addition. Under optimal conditions, the method has a low detection limit (1.35 × 10
2
CFU mL
−1
) and a linear response in the range 10
2
to 10
6
CFU mL
−1
.
Graphical abstract
The manuscripts describe a colorimetric method for the detection of
ETEC K88
by using intermolecular G-quadruplex to induce the agglomeration of gold nanoparticles, which can be directly used to determine the presence of bacteria with our naked eyes.</description><subject>Analytical Chemistry</subject><subject>Bacteria</subject><subject>Binding</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Colorimetry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Gold</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Optical density</subject><subject>Original Paper</subject><subject>Strands</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kU9rFDEYh4Modq1-AQ8S8OIl9c3_mWMpbRULXvQcsklmN2Um2SYzuP32Zp2q4EFyCMn7PC8_-CH0lsIFBdAfK4ACQYABAcF6So7P0IYKrogEzZ-jDQBThCvNztCrWu8BqFZMvERnnHGpKOcbNN6Sh8X6shzGcCRbW4PHtlb7iF2etjG1548477E9zHYKBdvk8S6PHieb8sGWOboxVDzkgq-r24cS3T7aJo8Rf-k67MMcyhSTnWNOr9GLwY41vHm6z9H3m-tvV5_I3dfbz1eXd8QJKmbSOSnsAFTxwLTwAiRzfS-ZhsAFdYxy3XEKHlTXW0ZBtpFjknpGnRrslp-jD-veQ8kPS6izmWJ1YRxtCnmphvFeK6V4Jxv6_h_0Pi8ltXQnSvXQUX6iLlZqZ8dgYhryXKxrx4cpupzCENv_paYSpOYdbwJbBVdyrSUM5lDiZMujoWBO5Zm1PNPKM7_KM8cmvXvKsmyn4P8ov9tqAF-B2kZpF8rfsP9Z-xPKEaNO</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Wang, Zefeng</creator><creator>Lu, Qiujun</creator><creator>Xu, Tao</creator><creator>Wang, Feiying</creator><creator>Huang, Fangfang</creator><creator>Peng, Yanling</creator><creator>Deng, Le</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20200501</creationdate><title>G-quadruplex-based assay combined with aptamer and gold nanoparticles for Escherichia coli K88 determination</title><author>Wang, Zefeng ; Lu, Qiujun ; Xu, Tao ; Wang, Feiying ; Huang, Fangfang ; Peng, Yanling ; Deng, Le</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-8c54af0163e274d4052c995270e341c21378310d0689a2105527c251d21c6fab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analytical Chemistry</topic><topic>Bacteria</topic><topic>Binding</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Colorimetry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Gold</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Optical density</topic><topic>Original Paper</topic><topic>Strands</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Zefeng</creatorcontrib><creatorcontrib>Lu, Qiujun</creatorcontrib><creatorcontrib>Xu, Tao</creatorcontrib><creatorcontrib>Wang, Feiying</creatorcontrib><creatorcontrib>Huang, Fangfang</creatorcontrib><creatorcontrib>Peng, Yanling</creatorcontrib><creatorcontrib>Deng, Le</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Zefeng</au><au>Lu, Qiujun</au><au>Xu, Tao</au><au>Wang, Feiying</au><au>Huang, Fangfang</au><au>Peng, Yanling</au><au>Deng, Le</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>G-quadruplex-based assay combined with aptamer and gold nanoparticles for Escherichia coli K88 determination</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2020-05-01</date><risdate>2020</risdate><volume>187</volume><issue>5</issue><spage>308</spage><epage>308</epage><pages>308-308</pages><artnum>308</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>A colorimetric method was developed using G-quadruplex and gold nanoparticles (AuNPs) for determination of
Escherichia coli K88
(
ETEC K88
). It was composed of two modules: (1) an aptamer as biorecognizing element and (2) a capturing DNA (modified with AuNPs at 5′) as a transducer. In the absence of target bacteria, the aptamer can form stable double strands with capturing DNA, preventing the binding of capturing DNA to the G-quadruplex. However, the double strands of capturing DNA and aptamer are untied due to the stronger binding of aptamers to bacteria in the presence of target bacteria. As a result, the G-quadruplex binds to capture DNA and leads to the aggregation and color change of AuNPs, which can be monitored by a spectrophotometer or visualization. The quantitative determination was achieved by monitoring the optical density change of AuNPs solution at 524 nm after target addition. Under optimal conditions, the method has a low detection limit (1.35 × 10
2
CFU mL
−1
) and a linear response in the range 10
2
to 10
6
CFU mL
−1
.
Graphical abstract
The manuscripts describe a colorimetric method for the detection of
ETEC K88
by using intermolecular G-quadruplex to induce the agglomeration of gold nanoparticles, which can be directly used to determine the presence of bacteria with our naked eyes.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>32356133</pmid><doi>10.1007/s00604-020-04291-x</doi><tpages>1</tpages></addata></record> |
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| ispartof | Mikrochimica acta (1966), 2020-05, Vol.187 (5), p.308-308, Article 308 |
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| subjects | Analytical Chemistry Bacteria Binding Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Colorimetry Deoxyribonucleic acid DNA E coli Escherichia coli Gold Microengineering Nanochemistry Nanoparticles Nanotechnology Optical density Original Paper Strands |
| title | G-quadruplex-based assay combined with aptamer and gold nanoparticles for Escherichia coli K88 determination |
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