Immunomodulatory and Tissue-preserving Effects of Human Dental Follicle Stem Cells in a Rat Cecal Ligation and Perforation Sepsis Model
Mesenchymal stem cells may be used for the treatment of sepsis. Dental follicle stem cells (DFSCs) are easily accessible but have not been studied in vivo or in clinical trials in sepsis models. We aim to elucidate DFSC effects on host immunological functions in a rat cecal ligation and perforation...
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| Veröffentlicht in: | Archives of medical research 2020-07, Vol.51 (5), p.397-405 |
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| creator | Topcu Sarica, Leyla Zibandeh, Noushin Genç, Deniz Gül, Fethi Akkoç, Tolga Kombak, Erdem Faruk Cinel, Leyla Akkoç, Tunç Cinel, Ismail |
| description | Mesenchymal stem cells may be used for the treatment of sepsis. Dental follicle stem cells (DFSCs) are easily accessible but have not been studied in vivo or in clinical trials in sepsis models.
We aim to elucidate DFSC effects on host immunological functions in a rat cecal ligation and perforation (CLP) sepsis model.
Adult male rats were categorized into group 1 (sham procedure SP), group 2 (SP + 1 × 106 DFSCs administered 0 h after SP), group 3 (CLP + saline), group 4 (CLP + 1 × 106 DFSCs administered 0 h after CLP), and group 5 (CLP + 1 × 106 DFSCs administered 4 h after CLP). Green fluorescent protein–labeled cells were used for imaging. Histopathological examination of ileal tissues was performed.
A significant increase in the percentage of CD4+/CD25+/Foxp3+ Treg cells in groups 4 and 5 occurred compared with that in group 3. No significant changes in CD3+/CD4+ helper T-cells and CD3+/CD8+ cytotoxic T-cells were observed. Treatment with DFSCs at 4 h significantly decreased the level of TNF-α compared with that in group 3. No significant changes in IL-10 levels and lymphocyte proliferation suppression were observed. During histopathological examination, no high scoring (Chiu scores: 3 or 4) rats were observed in the curative treatment group (group 5).
Treatment with DFSC after 4 h of sepsis induction downregulates tissue inflammatory responses by decreasing TNF-α levels and increasing Treg cell ratio. This also has a protective effect on intestinal tissues during sepsis. |
| doi_str_mv | 10.1016/j.arcmed.2020.04.010 |
| format | Article |
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We aim to elucidate DFSC effects on host immunological functions in a rat cecal ligation and perforation (CLP) sepsis model.
Adult male rats were categorized into group 1 (sham procedure SP), group 2 (SP + 1 × 106 DFSCs administered 0 h after SP), group 3 (CLP + saline), group 4 (CLP + 1 × 106 DFSCs administered 0 h after CLP), and group 5 (CLP + 1 × 106 DFSCs administered 4 h after CLP). Green fluorescent protein–labeled cells were used for imaging. Histopathological examination of ileal tissues was performed.
A significant increase in the percentage of CD4+/CD25+/Foxp3+ Treg cells in groups 4 and 5 occurred compared with that in group 3. No significant changes in CD3+/CD4+ helper T-cells and CD3+/CD8+ cytotoxic T-cells were observed. Treatment with DFSCs at 4 h significantly decreased the level of TNF-α compared with that in group 3. No significant changes in IL-10 levels and lymphocyte proliferation suppression were observed. During histopathological examination, no high scoring (Chiu scores: 3 or 4) rats were observed in the curative treatment group (group 5).
Treatment with DFSC after 4 h of sepsis induction downregulates tissue inflammatory responses by decreasing TNF-α levels and increasing Treg cell ratio. This also has a protective effect on intestinal tissues during sepsis.</description><identifier>ISSN: 0188-4409</identifier><identifier>EISSN: 1873-5487</identifier><identifier>DOI: 10.1016/j.arcmed.2020.04.010</identifier><identifier>PMID: 32334851</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Dental follicle ; Histopathology ; Immunomodulation ; Mesenchymal stem cells ; Sepsis</subject><ispartof>Archives of medical research, 2020-07, Vol.51 (5), p.397-405</ispartof><rights>2020 IMSS</rights><rights>Copyright © 2020 IMSS. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-f028703b47423a3285b4d383c6ef364d3ec764962d8e6d274b92e905ee88f91b3</citedby><cites>FETCH-LOGICAL-c362t-f028703b47423a3285b4d383c6ef364d3ec764962d8e6d274b92e905ee88f91b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.arcmed.2020.04.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32334851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Topcu Sarica, Leyla</creatorcontrib><creatorcontrib>Zibandeh, Noushin</creatorcontrib><creatorcontrib>Genç, Deniz</creatorcontrib><creatorcontrib>Gül, Fethi</creatorcontrib><creatorcontrib>Akkoç, Tolga</creatorcontrib><creatorcontrib>Kombak, Erdem Faruk</creatorcontrib><creatorcontrib>Cinel, Leyla</creatorcontrib><creatorcontrib>Akkoç, Tunç</creatorcontrib><creatorcontrib>Cinel, Ismail</creatorcontrib><title>Immunomodulatory and Tissue-preserving Effects of Human Dental Follicle Stem Cells in a Rat Cecal Ligation and Perforation Sepsis Model</title><title>Archives of medical research</title><addtitle>Arch Med Res</addtitle><description>Mesenchymal stem cells may be used for the treatment of sepsis. Dental follicle stem cells (DFSCs) are easily accessible but have not been studied in vivo or in clinical trials in sepsis models.
We aim to elucidate DFSC effects on host immunological functions in a rat cecal ligation and perforation (CLP) sepsis model.
Adult male rats were categorized into group 1 (sham procedure SP), group 2 (SP + 1 × 106 DFSCs administered 0 h after SP), group 3 (CLP + saline), group 4 (CLP + 1 × 106 DFSCs administered 0 h after CLP), and group 5 (CLP + 1 × 106 DFSCs administered 4 h after CLP). Green fluorescent protein–labeled cells were used for imaging. Histopathological examination of ileal tissues was performed.
A significant increase in the percentage of CD4+/CD25+/Foxp3+ Treg cells in groups 4 and 5 occurred compared with that in group 3. No significant changes in CD3+/CD4+ helper T-cells and CD3+/CD8+ cytotoxic T-cells were observed. Treatment with DFSCs at 4 h significantly decreased the level of TNF-α compared with that in group 3. No significant changes in IL-10 levels and lymphocyte proliferation suppression were observed. During histopathological examination, no high scoring (Chiu scores: 3 or 4) rats were observed in the curative treatment group (group 5).
Treatment with DFSC after 4 h of sepsis induction downregulates tissue inflammatory responses by decreasing TNF-α levels and increasing Treg cell ratio. This also has a protective effect on intestinal tissues during sepsis.</description><subject>Dental follicle</subject><subject>Histopathology</subject><subject>Immunomodulation</subject><subject>Mesenchymal stem cells</subject><subject>Sepsis</subject><issn>0188-4409</issn><issn>1873-5487</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u1DAQxy1ERZfCGyDkI5cEf8VxLkho29JKi1q15Ww5zrjyKrEXO6nUJ-C18ZL2ymlmNL_5_CP0iZKaEiq_7muT7ARDzQgjNRE1oeQN2lDV8qoRqn2LNoQqVQlBulP0Puc9IUQJ2b5Dp5xxLlRDN-jP9TQtIU5xWEYzx_SMTRjwg895geqQIEN68uERXzgHds44Ony1TCbgcwizGfFlHEdvR8D3M0x4C-OYsQ_Y4Dszl9AWZOcfzexj-Nf5FpKLaY3v4ZB9xj_jAOMHdOLMmOHjiz1Dvy4vHrZX1e7mx_X2-66yXLK5coSplvBetIJxw5lqejFwxa0Ex2VxwbZSdJINCuTAWtF3DDrSACjlOtrzM_Rl7XtI8fcCedaTz7asbQLEJWvGu4ZJSltRULGiNsWcEzh9SH4y6VlToo8S6L1eJdBHCTQRukhQyj6_TFj6Y-616PXnBfi2AlDufPKQdLYegoXBp_JjPUT__wl_AWEwmc8</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Topcu Sarica, Leyla</creator><creator>Zibandeh, Noushin</creator><creator>Genç, Deniz</creator><creator>Gül, Fethi</creator><creator>Akkoç, Tolga</creator><creator>Kombak, Erdem Faruk</creator><creator>Cinel, Leyla</creator><creator>Akkoç, Tunç</creator><creator>Cinel, Ismail</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200701</creationdate><title>Immunomodulatory and Tissue-preserving Effects of Human Dental Follicle Stem Cells in a Rat Cecal Ligation and Perforation Sepsis Model</title><author>Topcu Sarica, Leyla ; Zibandeh, Noushin ; Genç, Deniz ; Gül, Fethi ; Akkoç, Tolga ; Kombak, Erdem Faruk ; Cinel, Leyla ; Akkoç, Tunç ; Cinel, Ismail</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-f028703b47423a3285b4d383c6ef364d3ec764962d8e6d274b92e905ee88f91b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Dental follicle</topic><topic>Histopathology</topic><topic>Immunomodulation</topic><topic>Mesenchymal stem cells</topic><topic>Sepsis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Topcu Sarica, Leyla</creatorcontrib><creatorcontrib>Zibandeh, Noushin</creatorcontrib><creatorcontrib>Genç, Deniz</creatorcontrib><creatorcontrib>Gül, Fethi</creatorcontrib><creatorcontrib>Akkoç, Tolga</creatorcontrib><creatorcontrib>Kombak, Erdem Faruk</creatorcontrib><creatorcontrib>Cinel, Leyla</creatorcontrib><creatorcontrib>Akkoç, Tunç</creatorcontrib><creatorcontrib>Cinel, Ismail</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of medical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Topcu Sarica, Leyla</au><au>Zibandeh, Noushin</au><au>Genç, Deniz</au><au>Gül, Fethi</au><au>Akkoç, Tolga</au><au>Kombak, Erdem Faruk</au><au>Cinel, Leyla</au><au>Akkoç, Tunç</au><au>Cinel, Ismail</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunomodulatory and Tissue-preserving Effects of Human Dental Follicle Stem Cells in a Rat Cecal Ligation and Perforation Sepsis Model</atitle><jtitle>Archives of medical research</jtitle><addtitle>Arch Med Res</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>51</volume><issue>5</issue><spage>397</spage><epage>405</epage><pages>397-405</pages><issn>0188-4409</issn><eissn>1873-5487</eissn><abstract>Mesenchymal stem cells may be used for the treatment of sepsis. Dental follicle stem cells (DFSCs) are easily accessible but have not been studied in vivo or in clinical trials in sepsis models.
We aim to elucidate DFSC effects on host immunological functions in a rat cecal ligation and perforation (CLP) sepsis model.
Adult male rats were categorized into group 1 (sham procedure SP), group 2 (SP + 1 × 106 DFSCs administered 0 h after SP), group 3 (CLP + saline), group 4 (CLP + 1 × 106 DFSCs administered 0 h after CLP), and group 5 (CLP + 1 × 106 DFSCs administered 4 h after CLP). Green fluorescent protein–labeled cells were used for imaging. Histopathological examination of ileal tissues was performed.
A significant increase in the percentage of CD4+/CD25+/Foxp3+ Treg cells in groups 4 and 5 occurred compared with that in group 3. No significant changes in CD3+/CD4+ helper T-cells and CD3+/CD8+ cytotoxic T-cells were observed. Treatment with DFSCs at 4 h significantly decreased the level of TNF-α compared with that in group 3. No significant changes in IL-10 levels and lymphocyte proliferation suppression were observed. During histopathological examination, no high scoring (Chiu scores: 3 or 4) rats were observed in the curative treatment group (group 5).
Treatment with DFSC after 4 h of sepsis induction downregulates tissue inflammatory responses by decreasing TNF-α levels and increasing Treg cell ratio. This also has a protective effect on intestinal tissues during sepsis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>32334851</pmid><doi>10.1016/j.arcmed.2020.04.010</doi><tpages>9</tpages></addata></record> |
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| subjects | Dental follicle Histopathology Immunomodulation Mesenchymal stem cells Sepsis |
| title | Immunomodulatory and Tissue-preserving Effects of Human Dental Follicle Stem Cells in a Rat Cecal Ligation and Perforation Sepsis Model |
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