Bushenhuoxue formula promotes osteogenic differentiation of growth plate chondrocytes through β-catenin-dependent manner during osteoporosis
[Display omitted] Bushenhuoxue formula (BSHXF) has shown excellent clinical effects on the treatment of osteoporosis in China. The aim of this study is to determine the anti-osteoporosis effects and precise molecular mechanisms of BSHXF on mouse models. Ten-week-old female C57BL/6 J mice were subjec...
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| Veröffentlicht in: | Biomedicine & pharmacotherapy 2020-07, Vol.127, p.110170-110170, Article 110170 |
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| creator | Xia, Chenjie Zou, Zhen Fang, Liang Ge, Qinwen Zhang, Peng Xu, Huihui Xu, Rui Shi, Zhenyu Lin, Houfu Ding, Xinyi Xiao, Luwei Tong, Peijian Wang, Ping-er Jin, Hongting |
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Bushenhuoxue formula (BSHXF) has shown excellent clinical effects on the treatment of osteoporosis in China. The aim of this study is to determine the anti-osteoporosis effects and precise molecular mechanisms of BSHXF on mouse models.
Ten-week-old female C57BL/6 J mice were subjected to ovariectomy and provided a daily treatment of BSHXF. At 8 weeks post-surgery, the femurs were harvested for tissue analyses including μCT, histology, qRT-PCR and immunohistochemical (IHC) staining of β-catenin, ALP and FABP4. To investigate the role of β-catenin in the anti-osteoporosis effects of BSHXF, relative experiments mentioned above were performed in β-catenin conditional knockout mice.
Ovariectomized (OVX) mice presented severe bone loss and excessive fat accumulation in the chondro-osseous junction underneath the growth plate, with decreased expression of ALP and increased expression of FABP4. BSHXF significantly recovered the OVX-induced abnormal osteogenesis and adipogenesis with the activation of β-catenin in growth plate chondrocytes. Further, we generated growth plate chondrocyte-specific β-catenin knockout (β-cateninGli1ER) mice that exhibited bone loss and fat accumulation in the chondro-osseous junction, similar to the OVX mice. However, BSHXF failed to rescue the osteoporosis-like phenotype in β-cateninGli1ER mice, indicating the anti-osteoporosis effects of BSHXF act mainly through β-catenin signaling. No significant restoration of ALP and FABP4 was observed in β-cateninGli1ER mice after the treatment of BSHXF.
BSHXF attenuates osteoporosis by promoting osteogenic differentiation of growth plate chondrocytes mainly in β-catenin-dependent manner. BSHXF is considered as a new candidate for the treatment of osteoporosis. |
| doi_str_mv | 10.1016/j.biopha.2020.110170 |
| format | Article |
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Bushenhuoxue formula (BSHXF) has shown excellent clinical effects on the treatment of osteoporosis in China. The aim of this study is to determine the anti-osteoporosis effects and precise molecular mechanisms of BSHXF on mouse models.
Ten-week-old female C57BL/6 J mice were subjected to ovariectomy and provided a daily treatment of BSHXF. At 8 weeks post-surgery, the femurs were harvested for tissue analyses including μCT, histology, qRT-PCR and immunohistochemical (IHC) staining of β-catenin, ALP and FABP4. To investigate the role of β-catenin in the anti-osteoporosis effects of BSHXF, relative experiments mentioned above were performed in β-catenin conditional knockout mice.
Ovariectomized (OVX) mice presented severe bone loss and excessive fat accumulation in the chondro-osseous junction underneath the growth plate, with decreased expression of ALP and increased expression of FABP4. BSHXF significantly recovered the OVX-induced abnormal osteogenesis and adipogenesis with the activation of β-catenin in growth plate chondrocytes. Further, we generated growth plate chondrocyte-specific β-catenin knockout (β-cateninGli1ER) mice that exhibited bone loss and fat accumulation in the chondro-osseous junction, similar to the OVX mice. However, BSHXF failed to rescue the osteoporosis-like phenotype in β-cateninGli1ER mice, indicating the anti-osteoporosis effects of BSHXF act mainly through β-catenin signaling. No significant restoration of ALP and FABP4 was observed in β-cateninGli1ER mice after the treatment of BSHXF.
BSHXF attenuates osteoporosis by promoting osteogenic differentiation of growth plate chondrocytes mainly in β-catenin-dependent manner. BSHXF is considered as a new candidate for the treatment of osteoporosis.</description><identifier>ISSN: 0753-3322</identifier><identifier>EISSN: 1950-6007</identifier><identifier>DOI: 10.1016/j.biopha.2020.110170</identifier><identifier>PMID: 32334373</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Adipogenesis - drug effects ; Animals ; beta Catenin - genetics ; beta Catenin - metabolism ; Bushenhuoxue formula ; Cell differentiation ; Cell Differentiation - drug effects ; Chondrocytes - cytology ; Chondrocytes - drug effects ; Drugs, Chinese Herbal - pharmacology ; Female ; Growth Plate - metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Osteogenesis - drug effects ; Osteoporosis ; Osteoporosis - drug therapy ; Osteoporosis - pathology ; Ovariectomy ; Wnt Signaling Pathway - drug effects ; β-catenin</subject><ispartof>Biomedicine & pharmacotherapy, 2020-07, Vol.127, p.110170-110170, Article 110170</ispartof><rights>2020 The Author(s)</rights><rights>Copyright © 2020 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-86e7ab2fe515bb44cfa0ced31b5cc8d2f260f43b932e33569b0f340504a230943</citedby><cites>FETCH-LOGICAL-c408t-86e7ab2fe515bb44cfa0ced31b5cc8d2f260f43b932e33569b0f340504a230943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biopha.2020.110170$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32334373$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xia, Chenjie</creatorcontrib><creatorcontrib>Zou, Zhen</creatorcontrib><creatorcontrib>Fang, Liang</creatorcontrib><creatorcontrib>Ge, Qinwen</creatorcontrib><creatorcontrib>Zhang, Peng</creatorcontrib><creatorcontrib>Xu, Huihui</creatorcontrib><creatorcontrib>Xu, Rui</creatorcontrib><creatorcontrib>Shi, Zhenyu</creatorcontrib><creatorcontrib>Lin, Houfu</creatorcontrib><creatorcontrib>Ding, Xinyi</creatorcontrib><creatorcontrib>Xiao, Luwei</creatorcontrib><creatorcontrib>Tong, Peijian</creatorcontrib><creatorcontrib>Wang, Ping-er</creatorcontrib><creatorcontrib>Jin, Hongting</creatorcontrib><title>Bushenhuoxue formula promotes osteogenic differentiation of growth plate chondrocytes through β-catenin-dependent manner during osteoporosis</title><title>Biomedicine & pharmacotherapy</title><addtitle>Biomed Pharmacother</addtitle><description>[Display omitted]
Bushenhuoxue formula (BSHXF) has shown excellent clinical effects on the treatment of osteoporosis in China. The aim of this study is to determine the anti-osteoporosis effects and precise molecular mechanisms of BSHXF on mouse models.
Ten-week-old female C57BL/6 J mice were subjected to ovariectomy and provided a daily treatment of BSHXF. At 8 weeks post-surgery, the femurs were harvested for tissue analyses including μCT, histology, qRT-PCR and immunohistochemical (IHC) staining of β-catenin, ALP and FABP4. To investigate the role of β-catenin in the anti-osteoporosis effects of BSHXF, relative experiments mentioned above were performed in β-catenin conditional knockout mice.
Ovariectomized (OVX) mice presented severe bone loss and excessive fat accumulation in the chondro-osseous junction underneath the growth plate, with decreased expression of ALP and increased expression of FABP4. BSHXF significantly recovered the OVX-induced abnormal osteogenesis and adipogenesis with the activation of β-catenin in growth plate chondrocytes. Further, we generated growth plate chondrocyte-specific β-catenin knockout (β-cateninGli1ER) mice that exhibited bone loss and fat accumulation in the chondro-osseous junction, similar to the OVX mice. However, BSHXF failed to rescue the osteoporosis-like phenotype in β-cateninGli1ER mice, indicating the anti-osteoporosis effects of BSHXF act mainly through β-catenin signaling. No significant restoration of ALP and FABP4 was observed in β-cateninGli1ER mice after the treatment of BSHXF.
BSHXF attenuates osteoporosis by promoting osteogenic differentiation of growth plate chondrocytes mainly in β-catenin-dependent manner. BSHXF is considered as a new candidate for the treatment of osteoporosis.</description><subject>Adipogenesis - drug effects</subject><subject>Animals</subject><subject>beta Catenin - genetics</subject><subject>beta Catenin - metabolism</subject><subject>Bushenhuoxue formula</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - drug effects</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - drug effects</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Female</subject><subject>Growth Plate - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Osteogenesis - drug effects</subject><subject>Osteoporosis</subject><subject>Osteoporosis - drug therapy</subject><subject>Osteoporosis - pathology</subject><subject>Ovariectomy</subject><subject>Wnt Signaling Pathway - drug effects</subject><subject>β-catenin</subject><issn>0753-3322</issn><issn>1950-6007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctu1TAQtRAVvRT-ACEv2eTieJzXBgkqKEiVuoG15TjjG18ldrAd2n4EP9MP6TfhqxSWrEaaOWfOnDmEvCnZvmRl_f64761fRrXnjOdW7jXsGdmVXcWKmrHmOdmxpoICgPNz8jLGI2OsqqF9Qc6BAwhoYEd-f1rjiG5c_d2K1Pgwr5OiS_CzTxipjwn9AZ3VdLDGYECXrErWO-oNPQR_m0a6TCoh1aN3Q_D6_sRLY_DrYaSPD4XOQ2ddMeCCbsh8OivnMNBhDdYdNonFBx9tfEXOjJoivn6qF-THl8_fL78W1zdX3y4_XhdasDYVbY2N6rnBqqz6XghtFNM4QNlXWrcDN7xmRkDfAUeAqu56ZkCwignFgXUCLsi7bW82-nPFmORso8ZpUg79GiWHTrQt5JdmqNigOl8YAxq5BDurcC9LJk9ByKPcgpCnIOQWRKa9fVJY-xmHf6S_n8-ADxsAs89fFoOM2qLLNmxAneTg7f8V_gAGLaCK</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Xia, Chenjie</creator><creator>Zou, Zhen</creator><creator>Fang, Liang</creator><creator>Ge, Qinwen</creator><creator>Zhang, Peng</creator><creator>Xu, Huihui</creator><creator>Xu, Rui</creator><creator>Shi, Zhenyu</creator><creator>Lin, Houfu</creator><creator>Ding, Xinyi</creator><creator>Xiao, Luwei</creator><creator>Tong, Peijian</creator><creator>Wang, Ping-er</creator><creator>Jin, Hongting</creator><general>Elsevier Masson SAS</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202007</creationdate><title>Bushenhuoxue formula promotes osteogenic differentiation of growth plate chondrocytes through β-catenin-dependent manner during osteoporosis</title><author>Xia, Chenjie ; Zou, Zhen ; Fang, Liang ; Ge, Qinwen ; Zhang, Peng ; Xu, Huihui ; Xu, Rui ; Shi, Zhenyu ; Lin, Houfu ; Ding, Xinyi ; Xiao, Luwei ; Tong, Peijian ; Wang, Ping-er ; Jin, Hongting</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-86e7ab2fe515bb44cfa0ced31b5cc8d2f260f43b932e33569b0f340504a230943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adipogenesis - drug effects</topic><topic>Animals</topic><topic>beta Catenin - genetics</topic><topic>beta Catenin - metabolism</topic><topic>Bushenhuoxue formula</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - drug effects</topic><topic>Chondrocytes - cytology</topic><topic>Chondrocytes - drug effects</topic><topic>Drugs, Chinese Herbal - pharmacology</topic><topic>Female</topic><topic>Growth Plate - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Osteogenesis - drug effects</topic><topic>Osteoporosis</topic><topic>Osteoporosis - drug therapy</topic><topic>Osteoporosis - pathology</topic><topic>Ovariectomy</topic><topic>Wnt Signaling Pathway - drug effects</topic><topic>β-catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xia, Chenjie</creatorcontrib><creatorcontrib>Zou, Zhen</creatorcontrib><creatorcontrib>Fang, Liang</creatorcontrib><creatorcontrib>Ge, Qinwen</creatorcontrib><creatorcontrib>Zhang, Peng</creatorcontrib><creatorcontrib>Xu, Huihui</creatorcontrib><creatorcontrib>Xu, Rui</creatorcontrib><creatorcontrib>Shi, Zhenyu</creatorcontrib><creatorcontrib>Lin, Houfu</creatorcontrib><creatorcontrib>Ding, Xinyi</creatorcontrib><creatorcontrib>Xiao, Luwei</creatorcontrib><creatorcontrib>Tong, Peijian</creatorcontrib><creatorcontrib>Wang, Ping-er</creatorcontrib><creatorcontrib>Jin, Hongting</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedicine & pharmacotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xia, Chenjie</au><au>Zou, Zhen</au><au>Fang, Liang</au><au>Ge, Qinwen</au><au>Zhang, Peng</au><au>Xu, Huihui</au><au>Xu, Rui</au><au>Shi, Zhenyu</au><au>Lin, Houfu</au><au>Ding, Xinyi</au><au>Xiao, Luwei</au><au>Tong, Peijian</au><au>Wang, Ping-er</au><au>Jin, Hongting</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bushenhuoxue formula promotes osteogenic differentiation of growth plate chondrocytes through β-catenin-dependent manner during osteoporosis</atitle><jtitle>Biomedicine & pharmacotherapy</jtitle><addtitle>Biomed Pharmacother</addtitle><date>2020-07</date><risdate>2020</risdate><volume>127</volume><spage>110170</spage><epage>110170</epage><pages>110170-110170</pages><artnum>110170</artnum><issn>0753-3322</issn><eissn>1950-6007</eissn><abstract>[Display omitted]
Bushenhuoxue formula (BSHXF) has shown excellent clinical effects on the treatment of osteoporosis in China. The aim of this study is to determine the anti-osteoporosis effects and precise molecular mechanisms of BSHXF on mouse models.
Ten-week-old female C57BL/6 J mice were subjected to ovariectomy and provided a daily treatment of BSHXF. At 8 weeks post-surgery, the femurs were harvested for tissue analyses including μCT, histology, qRT-PCR and immunohistochemical (IHC) staining of β-catenin, ALP and FABP4. To investigate the role of β-catenin in the anti-osteoporosis effects of BSHXF, relative experiments mentioned above were performed in β-catenin conditional knockout mice.
Ovariectomized (OVX) mice presented severe bone loss and excessive fat accumulation in the chondro-osseous junction underneath the growth plate, with decreased expression of ALP and increased expression of FABP4. BSHXF significantly recovered the OVX-induced abnormal osteogenesis and adipogenesis with the activation of β-catenin in growth plate chondrocytes. Further, we generated growth plate chondrocyte-specific β-catenin knockout (β-cateninGli1ER) mice that exhibited bone loss and fat accumulation in the chondro-osseous junction, similar to the OVX mice. However, BSHXF failed to rescue the osteoporosis-like phenotype in β-cateninGli1ER mice, indicating the anti-osteoporosis effects of BSHXF act mainly through β-catenin signaling. No significant restoration of ALP and FABP4 was observed in β-cateninGli1ER mice after the treatment of BSHXF.
BSHXF attenuates osteoporosis by promoting osteogenic differentiation of growth plate chondrocytes mainly in β-catenin-dependent manner. BSHXF is considered as a new candidate for the treatment of osteoporosis.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>32334373</pmid><doi>10.1016/j.biopha.2020.110170</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adipogenesis - drug effects Animals beta Catenin - genetics beta Catenin - metabolism Bushenhuoxue formula Cell differentiation Cell Differentiation - drug effects Chondrocytes - cytology Chondrocytes - drug effects Drugs, Chinese Herbal - pharmacology Female Growth Plate - metabolism Mice Mice, Inbred C57BL Mice, Knockout Osteogenesis - drug effects Osteoporosis Osteoporosis - drug therapy Osteoporosis - pathology Ovariectomy Wnt Signaling Pathway - drug effects β-catenin |
| title | Bushenhuoxue formula promotes osteogenic differentiation of growth plate chondrocytes through β-catenin-dependent manner during osteoporosis |
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