MiR-139-5p inhibits the proliferation of gastric cancer cells by targeting Regulation of Nuclear Pre-mRNA Domain Containing 1B
Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B) has been of great interest in the field of oncology in recent years. The relationship between miRNAs and RPRD1B in gastric cancer (GC) has not been adequately reported. This study was designed to screen RPRD1B-targeted miRNAs and investiga...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2020-06, Vol.527 (2), p.393-400 |
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| creator | Wenquan, Liang Hongqing, Xi Yuhua, Liu Lili, Wang Wang, Zhang Ziwei, Zhuang Chuang, Wang Aizhen, Cai Xiaosong, Wu Bo, Wei Lin, Chen |
| description | Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B) has been of great interest in the field of oncology in recent years. The relationship between miRNAs and RPRD1B in gastric cancer (GC) has not been adequately reported. This study was designed to screen RPRD1B-targeted miRNAs and investigate its regulatory mechanism in GC cells. Quantitative RT-PCR and in situ hybridization were used to detect miRNA expression in GC tissues. Colony formation, EdU cell proliferation assay, and flow cytometry were used to analyze the cell cycle. Database-assisted gene expression analysis revealed that RPRD1B was targeted and regulated by miRNA-139-5p in GC. miRNA-139-5p expression was higher in GC tissue than in normal tissues and significantly correlated with tumor size, pathological stage, and disease-free survival of GC (p |
| doi_str_mv | 10.1016/j.bbrc.2020.04.067 |
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•MiR-139-5p expression is higher in cancer tissues than in paired normal tissues.•RPRD1B mRNA is the direct target of miR-139-5p in gastric cancer.•Gastric cancer cell proliferation regulation by miR-139-5p depends on RPRD1B.•A miR-139-5p/RPRD1B/Cyclin D1 axis regulates gastric cancer proliferation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2020.04.067</identifier><identifier>PMID: 32327260</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>biomarkers ; Cell Cycle Proteins - genetics ; Cell Line, Tumor ; Cell Proliferation ; cyclins ; D1 protein ; Disease Progression ; drugs ; flow cytometry ; Gastric cancer ; gene expression ; Gene Expression Regulation, Neoplastic ; Humans ; hybridization ; interphase ; microRNA ; MicroRNAs ; MicroRNAs - genetics ; Neoplasm Proteins - genetics ; protein synthesis ; Regulation of nuclear Pre-mRNA domain containing 1B ; stomach neoplasms ; Stomach Neoplasms - genetics ; Stomach Neoplasms - pathology</subject><ispartof>Biochemical and biophysical research communications, 2020-06, Vol.527 (2), p.393-400</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-d1ef8d6dad29894cd1527f22428b8f3203f277d2c98022c3305d2bde2bd55add3</citedby><cites>FETCH-LOGICAL-c389t-d1ef8d6dad29894cd1527f22428b8f3203f277d2c98022c3305d2bde2bd55add3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X20307932$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32327260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wenquan, Liang</creatorcontrib><creatorcontrib>Hongqing, Xi</creatorcontrib><creatorcontrib>Yuhua, Liu</creatorcontrib><creatorcontrib>Lili, Wang</creatorcontrib><creatorcontrib>Wang, Zhang</creatorcontrib><creatorcontrib>Ziwei, Zhuang</creatorcontrib><creatorcontrib>Chuang, Wang</creatorcontrib><creatorcontrib>Aizhen, Cai</creatorcontrib><creatorcontrib>Xiaosong, Wu</creatorcontrib><creatorcontrib>Bo, Wei</creatorcontrib><creatorcontrib>Lin, Chen</creatorcontrib><title>MiR-139-5p inhibits the proliferation of gastric cancer cells by targeting Regulation of Nuclear Pre-mRNA Domain Containing 1B</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B) has been of great interest in the field of oncology in recent years. The relationship between miRNAs and RPRD1B in gastric cancer (GC) has not been adequately reported. This study was designed to screen RPRD1B-targeted miRNAs and investigate its regulatory mechanism in GC cells. Quantitative RT-PCR and in situ hybridization were used to detect miRNA expression in GC tissues. Colony formation, EdU cell proliferation assay, and flow cytometry were used to analyze the cell cycle. Database-assisted gene expression analysis revealed that RPRD1B was targeted and regulated by miRNA-139-5p in GC. miRNA-139-5p expression was higher in GC tissue than in normal tissues and significantly correlated with tumor size, pathological stage, and disease-free survival of GC (p < 0.05). MiRNA-139-5p regulates GC cell proliferation and affects the transition from G1 to S phase. It binds explicitly to the 2013–2019 sites of the 3′UTR of RPRD1B and negatively regulates RPRD1B expression. We demonstrated that the ability of miR-139-5p to regulate GC cell proliferation depends on RPRD1B. This process is accompanied by changes in Cyclin D1 protein expression. We established a miR-139-5p/RPRD1B/tumor proliferation axis in GC, which may serve as novel biomarkers and drug targets for GC.
•MiR-139-5p expression is higher in cancer tissues than in paired normal tissues.•RPRD1B mRNA is the direct target of miR-139-5p in gastric cancer.•Gastric cancer cell proliferation regulation by miR-139-5p depends on RPRD1B.•A miR-139-5p/RPRD1B/Cyclin D1 axis regulates gastric cancer proliferation.</description><subject>biomarkers</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>cyclins</subject><subject>D1 protein</subject><subject>Disease Progression</subject><subject>drugs</subject><subject>flow cytometry</subject><subject>Gastric cancer</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>hybridization</subject><subject>interphase</subject><subject>microRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>Neoplasm Proteins - genetics</subject><subject>protein synthesis</subject><subject>Regulation of nuclear Pre-mRNA domain containing 1B</subject><subject>stomach neoplasms</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - pathology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvFDEQhC0EIpvAH-CAfOQyQ7s9T4lLWAgghYBWIHGzPHbPxqt5LLYHKRd-Ox5tyBEOrbp8VVJXMfZCQC5AVK8Pedd5kyMg5FDkUNWP2EZACxkKKB6zDQBUGbbixxk7D-EAIERRtU_ZmUSJNVawYb8_u10mZJuVR-6mW9e5GHi8JX708-B68jq6eeJzz_c6RO8MN3oy5LmhYQi8u-NR-z1FN-35jvbL8MDfLGYg7flXT9m4u7nk7-ZRu4lv5ykmXQ3i7TP2pNdDoOf3esG-X73_tv2YXX_58Gl7eZ0Z2bQxs4L6xlZWW2ybtjBWlFj3iAU2XdNLBNljXVs0bQOIRkooLXaW0pWltlZesFen3PTWz4VCVKML6wt6onkJCsuUJYtaNP9HZVs0qTxZJRRPqPFzCJ56dfRu1P5OCVDrROqg1onUOpGCQqWJkunlff7SjWQfLH83ScCbE0CpkF-OvArGUSrdOk8mKju7f-X_ATfGoW8</recordid><startdate>20200625</startdate><enddate>20200625</enddate><creator>Wenquan, Liang</creator><creator>Hongqing, Xi</creator><creator>Yuhua, Liu</creator><creator>Lili, Wang</creator><creator>Wang, Zhang</creator><creator>Ziwei, Zhuang</creator><creator>Chuang, Wang</creator><creator>Aizhen, Cai</creator><creator>Xiaosong, Wu</creator><creator>Bo, Wei</creator><creator>Lin, Chen</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20200625</creationdate><title>MiR-139-5p inhibits the proliferation of gastric cancer cells by targeting Regulation of Nuclear Pre-mRNA Domain Containing 1B</title><author>Wenquan, Liang ; Hongqing, Xi ; Yuhua, Liu ; Lili, Wang ; Wang, Zhang ; Ziwei, Zhuang ; Chuang, Wang ; Aizhen, Cai ; Xiaosong, Wu ; Bo, Wei ; Lin, Chen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-d1ef8d6dad29894cd1527f22428b8f3203f277d2c98022c3305d2bde2bd55add3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>biomarkers</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>cyclins</topic><topic>D1 protein</topic><topic>Disease Progression</topic><topic>drugs</topic><topic>flow cytometry</topic><topic>Gastric cancer</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>hybridization</topic><topic>interphase</topic><topic>microRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>Neoplasm Proteins - genetics</topic><topic>protein synthesis</topic><topic>Regulation of nuclear Pre-mRNA domain containing 1B</topic><topic>stomach neoplasms</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wenquan, Liang</creatorcontrib><creatorcontrib>Hongqing, Xi</creatorcontrib><creatorcontrib>Yuhua, Liu</creatorcontrib><creatorcontrib>Lili, Wang</creatorcontrib><creatorcontrib>Wang, Zhang</creatorcontrib><creatorcontrib>Ziwei, Zhuang</creatorcontrib><creatorcontrib>Chuang, Wang</creatorcontrib><creatorcontrib>Aizhen, Cai</creatorcontrib><creatorcontrib>Xiaosong, Wu</creatorcontrib><creatorcontrib>Bo, Wei</creatorcontrib><creatorcontrib>Lin, Chen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wenquan, Liang</au><au>Hongqing, Xi</au><au>Yuhua, Liu</au><au>Lili, Wang</au><au>Wang, Zhang</au><au>Ziwei, Zhuang</au><au>Chuang, Wang</au><au>Aizhen, Cai</au><au>Xiaosong, Wu</au><au>Bo, Wei</au><au>Lin, Chen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR-139-5p inhibits the proliferation of gastric cancer cells by targeting Regulation of Nuclear Pre-mRNA Domain Containing 1B</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2020-06-25</date><risdate>2020</risdate><volume>527</volume><issue>2</issue><spage>393</spage><epage>400</epage><pages>393-400</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B) has been of great interest in the field of oncology in recent years. The relationship between miRNAs and RPRD1B in gastric cancer (GC) has not been adequately reported. This study was designed to screen RPRD1B-targeted miRNAs and investigate its regulatory mechanism in GC cells. Quantitative RT-PCR and in situ hybridization were used to detect miRNA expression in GC tissues. Colony formation, EdU cell proliferation assay, and flow cytometry were used to analyze the cell cycle. Database-assisted gene expression analysis revealed that RPRD1B was targeted and regulated by miRNA-139-5p in GC. miRNA-139-5p expression was higher in GC tissue than in normal tissues and significantly correlated with tumor size, pathological stage, and disease-free survival of GC (p < 0.05). MiRNA-139-5p regulates GC cell proliferation and affects the transition from G1 to S phase. It binds explicitly to the 2013–2019 sites of the 3′UTR of RPRD1B and negatively regulates RPRD1B expression. We demonstrated that the ability of miR-139-5p to regulate GC cell proliferation depends on RPRD1B. This process is accompanied by changes in Cyclin D1 protein expression. We established a miR-139-5p/RPRD1B/tumor proliferation axis in GC, which may serve as novel biomarkers and drug targets for GC.
•MiR-139-5p expression is higher in cancer tissues than in paired normal tissues.•RPRD1B mRNA is the direct target of miR-139-5p in gastric cancer.•Gastric cancer cell proliferation regulation by miR-139-5p depends on RPRD1B.•A miR-139-5p/RPRD1B/Cyclin D1 axis regulates gastric cancer proliferation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>32327260</pmid><doi>10.1016/j.bbrc.2020.04.067</doi><tpages>8</tpages></addata></record> |
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| subjects | biomarkers Cell Cycle Proteins - genetics Cell Line, Tumor Cell Proliferation cyclins D1 protein Disease Progression drugs flow cytometry Gastric cancer gene expression Gene Expression Regulation, Neoplastic Humans hybridization interphase microRNA MicroRNAs MicroRNAs - genetics Neoplasm Proteins - genetics protein synthesis Regulation of nuclear Pre-mRNA domain containing 1B stomach neoplasms Stomach Neoplasms - genetics Stomach Neoplasms - pathology |
| title | MiR-139-5p inhibits the proliferation of gastric cancer cells by targeting Regulation of Nuclear Pre-mRNA Domain Containing 1B |
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