Irgm1 knockout indirectly inhibits regeneration after skeletal muscle injury in mice
•Irgm1−/− mice showed poor and delayed skeletal muscle injury repair.•Irgm1 knockout did not affect myoblast differentiation in vitro.•Irgm1−/− increased the levels of IFN-γ to suppress myoblast differentiation in vivo. Immunity-related GTPase family M1 protein (lRGM1) plays an important role in hos...
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| Veröffentlicht in: | International immunopharmacology 2020-07, Vol.84, p.106515-106515, Article 106515 |
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| container_title | International immunopharmacology |
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| creator | Zhang, Liulei Wang, Guangyou Chen, Xin Zhang, Chong Jiang, Yan Zhao, Wei Li, Hulun Sun, Jin Li, Xinrong Xu, Hao Weng, Yuting Zhang, Xiaoyu Hou, Lixuan Kong, Qingfei Liu, Yumei Xu, Hongwei Mu, Lili Wang, Jinghua |
| description | •Irgm1−/− mice showed poor and delayed skeletal muscle injury repair.•Irgm1 knockout did not affect myoblast differentiation in vitro.•Irgm1−/− increased the levels of IFN-γ to suppress myoblast differentiation in vivo.
Immunity-related GTPase family M1 protein (lRGM1) plays an important role in host resistance to infection, immune inflammation, and tumors, and it is expressed in various tissues and cells, including the central nervous system, cardiovascular system, bone marrow-derived cells, glioma, and melanoma. However, the effect of IRGM1 in the muscles has not been reported to date. In this study, Irgm1−/− mice were used to evaluate the effect of lrgm1 on regeneration after skeletal muscle injury. The tibialis anterior muscle in Irgm1−/− mice was poorly repaired after BaCl2-induced injury, whereas lrgm1 knockout itself had no significant effect on the differentiation of myoblasts. However, the microenvironment of Irgm1−/− mice with a high interferon-gamma level inhibited the differentiation of myoblasts in vivo. These results suggest that lrgm1 knockout indirectly inhibits skeletal muscle regeneration after injury, providing new insights into the biological function of IRGM1. |
| doi_str_mv | 10.1016/j.intimp.2020.106515 |
| format | Article |
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Immunity-related GTPase family M1 protein (lRGM1) plays an important role in host resistance to infection, immune inflammation, and tumors, and it is expressed in various tissues and cells, including the central nervous system, cardiovascular system, bone marrow-derived cells, glioma, and melanoma. However, the effect of IRGM1 in the muscles has not been reported to date. In this study, Irgm1−/− mice were used to evaluate the effect of lrgm1 on regeneration after skeletal muscle injury. The tibialis anterior muscle in Irgm1−/− mice was poorly repaired after BaCl2-induced injury, whereas lrgm1 knockout itself had no significant effect on the differentiation of myoblasts. However, the microenvironment of Irgm1−/− mice with a high interferon-gamma level inhibited the differentiation of myoblasts in vivo. These results suggest that lrgm1 knockout indirectly inhibits skeletal muscle regeneration after injury, providing new insights into the biological function of IRGM1.</description><identifier>ISSN: 1567-5769</identifier><identifier>EISSN: 1878-1705</identifier><identifier>DOI: 10.1016/j.intimp.2020.106515</identifier><identifier>PMID: 32311672</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Barium chloride ; Barium Compounds ; Bone marrow ; Brain tumors ; Cardiovascular system ; Cell Differentiation ; Cells, Cultured ; Central nervous system ; Chlorides ; Differentiation ; Glioma cells ; GTP-Binding Proteins - genetics ; GTP-Binding Proteins - physiology ; Injuries ; Interferon ; Interferon-gamma - physiology ; lrgm1 ; Male ; Melanoma ; Mice, Knockout ; Microenvironment ; Muscle regeneration ; Muscle, Skeletal - injuries ; Muscle, Skeletal - physiology ; Muscles ; Musculoskeletal system ; Myoblasts ; Regeneration ; Satellite Cells, Skeletal Muscle - physiology ; Skeletal muscle ; Skeletal muscle injury ; Tibialis anterior muscle ; Tumors ; γ-Interferon</subject><ispartof>International immunopharmacology, 2020-07, Vol.84, p.106515-106515, Article 106515</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Jul 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-57bfb5efa840fe23a75113bb80d293a725ed5bab961766ab6f1b2ced10532c5d3</citedby><cites>FETCH-LOGICAL-c390t-57bfb5efa840fe23a75113bb80d293a725ed5bab961766ab6f1b2ced10532c5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.intimp.2020.106515$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32311672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Liulei</creatorcontrib><creatorcontrib>Wang, Guangyou</creatorcontrib><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Zhang, Chong</creatorcontrib><creatorcontrib>Jiang, Yan</creatorcontrib><creatorcontrib>Zhao, Wei</creatorcontrib><creatorcontrib>Li, Hulun</creatorcontrib><creatorcontrib>Sun, Jin</creatorcontrib><creatorcontrib>Li, Xinrong</creatorcontrib><creatorcontrib>Xu, Hao</creatorcontrib><creatorcontrib>Weng, Yuting</creatorcontrib><creatorcontrib>Zhang, Xiaoyu</creatorcontrib><creatorcontrib>Hou, Lixuan</creatorcontrib><creatorcontrib>Kong, Qingfei</creatorcontrib><creatorcontrib>Liu, Yumei</creatorcontrib><creatorcontrib>Xu, Hongwei</creatorcontrib><creatorcontrib>Mu, Lili</creatorcontrib><creatorcontrib>Wang, Jinghua</creatorcontrib><title>Irgm1 knockout indirectly inhibits regeneration after skeletal muscle injury in mice</title><title>International immunopharmacology</title><addtitle>Int Immunopharmacol</addtitle><description>•Irgm1−/− mice showed poor and delayed skeletal muscle injury repair.•Irgm1 knockout did not affect myoblast differentiation in vitro.•Irgm1−/− increased the levels of IFN-γ to suppress myoblast differentiation in vivo.
Immunity-related GTPase family M1 protein (lRGM1) plays an important role in host resistance to infection, immune inflammation, and tumors, and it is expressed in various tissues and cells, including the central nervous system, cardiovascular system, bone marrow-derived cells, glioma, and melanoma. However, the effect of IRGM1 in the muscles has not been reported to date. In this study, Irgm1−/− mice were used to evaluate the effect of lrgm1 on regeneration after skeletal muscle injury. The tibialis anterior muscle in Irgm1−/− mice was poorly repaired after BaCl2-induced injury, whereas lrgm1 knockout itself had no significant effect on the differentiation of myoblasts. However, the microenvironment of Irgm1−/− mice with a high interferon-gamma level inhibited the differentiation of myoblasts in vivo. These results suggest that lrgm1 knockout indirectly inhibits skeletal muscle regeneration after injury, providing new insights into the biological function of IRGM1.</description><subject>Animals</subject><subject>Barium chloride</subject><subject>Barium Compounds</subject><subject>Bone marrow</subject><subject>Brain tumors</subject><subject>Cardiovascular system</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Central nervous system</subject><subject>Chlorides</subject><subject>Differentiation</subject><subject>Glioma cells</subject><subject>GTP-Binding Proteins - genetics</subject><subject>GTP-Binding Proteins - physiology</subject><subject>Injuries</subject><subject>Interferon</subject><subject>Interferon-gamma - physiology</subject><subject>lrgm1</subject><subject>Male</subject><subject>Melanoma</subject><subject>Mice, Knockout</subject><subject>Microenvironment</subject><subject>Muscle regeneration</subject><subject>Muscle, Skeletal - injuries</subject><subject>Muscle, Skeletal - physiology</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Myoblasts</subject><subject>Regeneration</subject><subject>Satellite Cells, Skeletal Muscle - physiology</subject><subject>Skeletal muscle</subject><subject>Skeletal muscle injury</subject><subject>Tibialis anterior muscle</subject><subject>Tumors</subject><subject>γ-Interferon</subject><issn>1567-5769</issn><issn>1878-1705</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMo1tsbiAy4cdOayyTpbAQp3kBwo-uQZM7UTOdSk4zQtzd1qgsXrnJy-M7Jnw-hc4JnBBNxXc9cF127nlFMty3BCd9DR2Qu51MiMd9PNRdyyqUoJug4hBrj1M_JIZowyggRkh6h1ye_bEm26nq76oeYua50HmxsNql8d8bFkHlYQgdeR9d3ma4i-CysoIGom6wdgm0gsfXgtyNZ6yycooNKNwHOducJeru_e108Tp9fHp4Wt89TywocUzJTGQ6Vnue4Asq05IQwY-a4pEW6UQ4lN9oUgkghtBEVMdRCSTBn1PKSnaCrce_a9x8DhKhaFyw0je6gH4KirGA45znNE3r5B637wXcpnaJ5nuJI-U3lI2V9H4KHSq29a7XfKILV1rqq1Whdba2r0Xoau9gtH0wL5e_Qj-YE3IwAJBufDrwK1kGX_vItW5W9-_-FLz8LlUc</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Zhang, Liulei</creator><creator>Wang, Guangyou</creator><creator>Chen, Xin</creator><creator>Zhang, Chong</creator><creator>Jiang, Yan</creator><creator>Zhao, Wei</creator><creator>Li, Hulun</creator><creator>Sun, Jin</creator><creator>Li, Xinrong</creator><creator>Xu, Hao</creator><creator>Weng, Yuting</creator><creator>Zhang, Xiaoyu</creator><creator>Hou, Lixuan</creator><creator>Kong, Qingfei</creator><creator>Liu, Yumei</creator><creator>Xu, Hongwei</creator><creator>Mu, Lili</creator><creator>Wang, Jinghua</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202007</creationdate><title>Irgm1 knockout indirectly inhibits regeneration after skeletal muscle injury in mice</title><author>Zhang, Liulei ; Wang, Guangyou ; Chen, Xin ; Zhang, Chong ; Jiang, Yan ; Zhao, Wei ; Li, Hulun ; Sun, Jin ; Li, Xinrong ; Xu, Hao ; Weng, Yuting ; Zhang, Xiaoyu ; Hou, Lixuan ; Kong, Qingfei ; Liu, Yumei ; Xu, Hongwei ; Mu, Lili ; Wang, Jinghua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-57bfb5efa840fe23a75113bb80d293a725ed5bab961766ab6f1b2ced10532c5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Barium chloride</topic><topic>Barium Compounds</topic><topic>Bone marrow</topic><topic>Brain tumors</topic><topic>Cardiovascular system</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Central nervous system</topic><topic>Chlorides</topic><topic>Differentiation</topic><topic>Glioma cells</topic><topic>GTP-Binding Proteins - genetics</topic><topic>GTP-Binding Proteins - physiology</topic><topic>Injuries</topic><topic>Interferon</topic><topic>Interferon-gamma - physiology</topic><topic>lrgm1</topic><topic>Male</topic><topic>Melanoma</topic><topic>Mice, Knockout</topic><topic>Microenvironment</topic><topic>Muscle regeneration</topic><topic>Muscle, Skeletal - injuries</topic><topic>Muscle, Skeletal - physiology</topic><topic>Muscles</topic><topic>Musculoskeletal system</topic><topic>Myoblasts</topic><topic>Regeneration</topic><topic>Satellite Cells, Skeletal Muscle - physiology</topic><topic>Skeletal muscle</topic><topic>Skeletal muscle injury</topic><topic>Tibialis anterior muscle</topic><topic>Tumors</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Liulei</creatorcontrib><creatorcontrib>Wang, Guangyou</creatorcontrib><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Zhang, Chong</creatorcontrib><creatorcontrib>Jiang, Yan</creatorcontrib><creatorcontrib>Zhao, Wei</creatorcontrib><creatorcontrib>Li, Hulun</creatorcontrib><creatorcontrib>Sun, Jin</creatorcontrib><creatorcontrib>Li, Xinrong</creatorcontrib><creatorcontrib>Xu, Hao</creatorcontrib><creatorcontrib>Weng, Yuting</creatorcontrib><creatorcontrib>Zhang, Xiaoyu</creatorcontrib><creatorcontrib>Hou, Lixuan</creatorcontrib><creatorcontrib>Kong, Qingfei</creatorcontrib><creatorcontrib>Liu, Yumei</creatorcontrib><creatorcontrib>Xu, Hongwei</creatorcontrib><creatorcontrib>Mu, Lili</creatorcontrib><creatorcontrib>Wang, Jinghua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Liulei</au><au>Wang, Guangyou</au><au>Chen, Xin</au><au>Zhang, Chong</au><au>Jiang, Yan</au><au>Zhao, Wei</au><au>Li, Hulun</au><au>Sun, Jin</au><au>Li, Xinrong</au><au>Xu, Hao</au><au>Weng, Yuting</au><au>Zhang, Xiaoyu</au><au>Hou, Lixuan</au><au>Kong, Qingfei</au><au>Liu, Yumei</au><au>Xu, Hongwei</au><au>Mu, Lili</au><au>Wang, Jinghua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Irgm1 knockout indirectly inhibits regeneration after skeletal muscle injury in mice</atitle><jtitle>International immunopharmacology</jtitle><addtitle>Int Immunopharmacol</addtitle><date>2020-07</date><risdate>2020</risdate><volume>84</volume><spage>106515</spage><epage>106515</epage><pages>106515-106515</pages><artnum>106515</artnum><issn>1567-5769</issn><eissn>1878-1705</eissn><abstract>•Irgm1−/− mice showed poor and delayed skeletal muscle injury repair.•Irgm1 knockout did not affect myoblast differentiation in vitro.•Irgm1−/− increased the levels of IFN-γ to suppress myoblast differentiation in vivo.
Immunity-related GTPase family M1 protein (lRGM1) plays an important role in host resistance to infection, immune inflammation, and tumors, and it is expressed in various tissues and cells, including the central nervous system, cardiovascular system, bone marrow-derived cells, glioma, and melanoma. However, the effect of IRGM1 in the muscles has not been reported to date. In this study, Irgm1−/− mice were used to evaluate the effect of lrgm1 on regeneration after skeletal muscle injury. The tibialis anterior muscle in Irgm1−/− mice was poorly repaired after BaCl2-induced injury, whereas lrgm1 knockout itself had no significant effect on the differentiation of myoblasts. However, the microenvironment of Irgm1−/− mice with a high interferon-gamma level inhibited the differentiation of myoblasts in vivo. These results suggest that lrgm1 knockout indirectly inhibits skeletal muscle regeneration after injury, providing new insights into the biological function of IRGM1.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>32311672</pmid><doi>10.1016/j.intimp.2020.106515</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals Barium chloride Barium Compounds Bone marrow Brain tumors Cardiovascular system Cell Differentiation Cells, Cultured Central nervous system Chlorides Differentiation Glioma cells GTP-Binding Proteins - genetics GTP-Binding Proteins - physiology Injuries Interferon Interferon-gamma - physiology lrgm1 Male Melanoma Mice, Knockout Microenvironment Muscle regeneration Muscle, Skeletal - injuries Muscle, Skeletal - physiology Muscles Musculoskeletal system Myoblasts Regeneration Satellite Cells, Skeletal Muscle - physiology Skeletal muscle Skeletal muscle injury Tibialis anterior muscle Tumors γ-Interferon |
| title | Irgm1 knockout indirectly inhibits regeneration after skeletal muscle injury in mice |
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