A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma
Objectives This research aimed to evaluate the antiangiogenic activity of isolated flavonoid 4a,5,8,8a‐tetrahydro‐5‐hydroxy‐3,7,8‐trimethoxy‐2‐(3,4‐dimethoxyphenyl) chromen‐4‐one (TMF) from Tabebuia chrysantha. STAT3‐MMP9 signalling is a signal transduction mechanism that promotes angiogenesis in va...
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| Veröffentlicht in: | Journal of pharmacy and pharmacology 2020-07, Vol.72 (7), p.990-999 |
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| container_title | Journal of pharmacy and pharmacology |
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| creator | Panda, Siva Prasad Panigrahy, Uttam Prasad Prasanth, DSNBK Gorla, Uma Sankar Guntupalli, Chakravarthi Panda, Deba Prasad Jena, Bikash Ranjan |
| description | Objectives
This research aimed to evaluate the antiangiogenic activity of isolated flavonoid 4a,5,8,8a‐tetrahydro‐5‐hydroxy‐3,7,8‐trimethoxy‐2‐(3,4‐dimethoxyphenyl) chromen‐4‐one (TMF) from Tabebuia chrysantha. STAT3‐MMP9 signalling is a signal transduction mechanism that promotes angiogenesis in various cancers.
Methods
The tumour xenografting chicken embryo chorioallantoic membrane (CAM) model‐based ex vivo assay was used to evaluate the activity of TMF. The Western blot, densitometric analysis and quantitative real‐time polymerase chain reaction (qRT‐PCR) were performed to evaluate the activity of the MMP9. Zebrafish embryos were used to evaluate embryotoxicity, and in vitro free radical scavenging activity of flavonoid was also elucidated.
Key findings
This research assessed the high level of STAT3, p‐ERK, VEGF‐R and MMP9 in the tissue extract of the control group, and also, the suppression of angiogenesis in the treatment groups was due to scavenged ROS and RNS, dephosphorylation of STAT3 and ERK, and suppression of MMP9 gene expression.
Conclusion
The isolated flavonoid named TMF from T. chrysantha functions as specific regulators of target proteins of angiosarcoma. The STAT3‐MMP9 signalling may be used as an effective prognostic marker of angiosarcoma. |
| doi_str_mv | 10.1111/jphp.13272 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2393043955</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2410523512</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4592-9229d936d3f8c1275360c2cdafa648186979f5c39fe5a20038d5fb25a6b9a62f3</originalsourceid><addsrcrecordid>eNp9kcFu1DAQhi0EokvhwgMgS1wqpLT2OHbiY1VRSlWJHtqz5Th216skDp6kdN-eLCkcOHQuo5G--TT6h5CPnJ3ypc5243Y85QIqeEU2wEooKi7r12TDGEAhZCWOyDvEHWOsUkq9JUcCxGGz3pDxnE459n7apqc9DZ19TEOKLY2YOjv5loaceoqT76l_mrJ1E02B3tnGN3O01G3zHu0wbS3FeRyzR_RI7fAQ04MfPEakcVhntNml3r4nb4Lt0H947sfk_vLr3cVVcfPj2_eL85vClVJDoQF0q4VqRagdh0oKxRy41garyprXSlc6SCd08NICY6JuZWhAWtVoqyCIY3Kyesecfs4eJ9NHdL7r7ODTjAaEFqwUWsoF_fwfuktzHpbrDJScSRCSw0J9WSmXE2L2wYxLcDbvDWfmkKY5_MH8-cMCf3pWzk3v23_o3-AXgK_Ar9j5_Qsqc317dbtKfwMCYpPl</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2410523512</pqid></control><display><type>article</type><title>A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma</title><source>Access via Wiley Online Library</source><source>MEDLINE</source><source>Oxford Journals</source><creator>Panda, Siva Prasad ; Panigrahy, Uttam Prasad ; Prasanth, DSNBK ; Gorla, Uma Sankar ; Guntupalli, Chakravarthi ; Panda, Deba Prasad ; Jena, Bikash Ranjan</creator><creatorcontrib>Panda, Siva Prasad ; Panigrahy, Uttam Prasad ; Prasanth, DSNBK ; Gorla, Uma Sankar ; Guntupalli, Chakravarthi ; Panda, Deba Prasad ; Jena, Bikash Ranjan</creatorcontrib><description>Objectives
This research aimed to evaluate the antiangiogenic activity of isolated flavonoid 4a,5,8,8a‐tetrahydro‐5‐hydroxy‐3,7,8‐trimethoxy‐2‐(3,4‐dimethoxyphenyl) chromen‐4‐one (TMF) from Tabebuia chrysantha. STAT3‐MMP9 signalling is a signal transduction mechanism that promotes angiogenesis in various cancers.
Methods
The tumour xenografting chicken embryo chorioallantoic membrane (CAM) model‐based ex vivo assay was used to evaluate the activity of TMF. The Western blot, densitometric analysis and quantitative real‐time polymerase chain reaction (qRT‐PCR) were performed to evaluate the activity of the MMP9. Zebrafish embryos were used to evaluate embryotoxicity, and in vitro free radical scavenging activity of flavonoid was also elucidated.
Key findings
This research assessed the high level of STAT3, p‐ERK, VEGF‐R and MMP9 in the tissue extract of the control group, and also, the suppression of angiogenesis in the treatment groups was due to scavenged ROS and RNS, dephosphorylation of STAT3 and ERK, and suppression of MMP9 gene expression.
Conclusion
The isolated flavonoid named TMF from T. chrysantha functions as specific regulators of target proteins of angiosarcoma. The STAT3‐MMP9 signalling may be used as an effective prognostic marker of angiosarcoma.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1111/jphp.13272</identifier><identifier>PMID: 32311118</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Angiogenesis ; Angiogenesis Inhibitors - pharmacology ; angiosarcoma ; Animals ; Cell Proliferation - drug effects ; Chick Embryo ; Chorioallantoic Membrane ; Dephosphorylation ; Embryos ; Flavonoids ; Flavonoids - pharmacology ; Gelatinase B ; Gene expression ; Hemangiosarcoma - drug therapy ; Matrix Metalloproteinase 9 - metabolism ; MMP9 ; Plant Extracts - pharmacology ; Polymerase chain reaction ; Signal transduction ; Signal Transduction - drug effects ; STAT3 ; Stat3 protein ; STAT3 Transcription Factor - metabolism ; Tabebuia ; Tabebuia chrysantha ; TMF ; Tumors ; Vascular endothelial growth factor ; Xenograft Model Antitumor Assays ; Xenografts</subject><ispartof>Journal of pharmacy and pharmacology, 2020-07, Vol.72 (7), p.990-999</ispartof><rights>2020 Royal Pharmaceutical Society</rights><rights>2020 Royal Pharmaceutical Society.</rights><rights>Copyright © 2020 Royal Pharmaceutical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4592-9229d936d3f8c1275360c2cdafa648186979f5c39fe5a20038d5fb25a6b9a62f3</citedby><cites>FETCH-LOGICAL-c4592-9229d936d3f8c1275360c2cdafa648186979f5c39fe5a20038d5fb25a6b9a62f3</cites><orcidid>0000-0002-6980-6761</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjphp.13272$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjphp.13272$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32311118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Panda, Siva Prasad</creatorcontrib><creatorcontrib>Panigrahy, Uttam Prasad</creatorcontrib><creatorcontrib>Prasanth, DSNBK</creatorcontrib><creatorcontrib>Gorla, Uma Sankar</creatorcontrib><creatorcontrib>Guntupalli, Chakravarthi</creatorcontrib><creatorcontrib>Panda, Deba Prasad</creatorcontrib><creatorcontrib>Jena, Bikash Ranjan</creatorcontrib><title>A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma</title><title>Journal of pharmacy and pharmacology</title><addtitle>J Pharm Pharmacol</addtitle><description>Objectives
This research aimed to evaluate the antiangiogenic activity of isolated flavonoid 4a,5,8,8a‐tetrahydro‐5‐hydroxy‐3,7,8‐trimethoxy‐2‐(3,4‐dimethoxyphenyl) chromen‐4‐one (TMF) from Tabebuia chrysantha. STAT3‐MMP9 signalling is a signal transduction mechanism that promotes angiogenesis in various cancers.
Methods
The tumour xenografting chicken embryo chorioallantoic membrane (CAM) model‐based ex vivo assay was used to evaluate the activity of TMF. The Western blot, densitometric analysis and quantitative real‐time polymerase chain reaction (qRT‐PCR) were performed to evaluate the activity of the MMP9. Zebrafish embryos were used to evaluate embryotoxicity, and in vitro free radical scavenging activity of flavonoid was also elucidated.
Key findings
This research assessed the high level of STAT3, p‐ERK, VEGF‐R and MMP9 in the tissue extract of the control group, and also, the suppression of angiogenesis in the treatment groups was due to scavenged ROS and RNS, dephosphorylation of STAT3 and ERK, and suppression of MMP9 gene expression.
Conclusion
The isolated flavonoid named TMF from T. chrysantha functions as specific regulators of target proteins of angiosarcoma. The STAT3‐MMP9 signalling may be used as an effective prognostic marker of angiosarcoma.</description><subject>Angiogenesis</subject><subject>Angiogenesis Inhibitors - pharmacology</subject><subject>angiosarcoma</subject><subject>Animals</subject><subject>Cell Proliferation - drug effects</subject><subject>Chick Embryo</subject><subject>Chorioallantoic Membrane</subject><subject>Dephosphorylation</subject><subject>Embryos</subject><subject>Flavonoids</subject><subject>Flavonoids - pharmacology</subject><subject>Gelatinase B</subject><subject>Gene expression</subject><subject>Hemangiosarcoma - drug therapy</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>MMP9</subject><subject>Plant Extracts - pharmacology</subject><subject>Polymerase chain reaction</subject><subject>Signal transduction</subject><subject>Signal Transduction - drug effects</subject><subject>STAT3</subject><subject>Stat3 protein</subject><subject>STAT3 Transcription Factor - metabolism</subject><subject>Tabebuia</subject><subject>Tabebuia chrysantha</subject><subject>TMF</subject><subject>Tumors</subject><subject>Vascular endothelial growth factor</subject><subject>Xenograft Model Antitumor Assays</subject><subject>Xenografts</subject><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EokvhwgMgS1wqpLT2OHbiY1VRSlWJHtqz5Th216skDp6kdN-eLCkcOHQuo5G--TT6h5CPnJ3ypc5243Y85QIqeEU2wEooKi7r12TDGEAhZCWOyDvEHWOsUkq9JUcCxGGz3pDxnE459n7apqc9DZ19TEOKLY2YOjv5loaceoqT76l_mrJ1E02B3tnGN3O01G3zHu0wbS3FeRyzR_RI7fAQ04MfPEakcVhntNml3r4nb4Lt0H947sfk_vLr3cVVcfPj2_eL85vClVJDoQF0q4VqRagdh0oKxRy41garyprXSlc6SCd08NICY6JuZWhAWtVoqyCIY3Kyesecfs4eJ9NHdL7r7ODTjAaEFqwUWsoF_fwfuktzHpbrDJScSRCSw0J9WSmXE2L2wYxLcDbvDWfmkKY5_MH8-cMCf3pWzk3v23_o3-AXgK_Ar9j5_Qsqc317dbtKfwMCYpPl</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Panda, Siva Prasad</creator><creator>Panigrahy, Uttam Prasad</creator><creator>Prasanth, DSNBK</creator><creator>Gorla, Uma Sankar</creator><creator>Guntupalli, Chakravarthi</creator><creator>Panda, Deba Prasad</creator><creator>Jena, Bikash Ranjan</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6980-6761</orcidid></search><sort><creationdate>202007</creationdate><title>A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma</title><author>Panda, Siva Prasad ; Panigrahy, Uttam Prasad ; Prasanth, DSNBK ; Gorla, Uma Sankar ; Guntupalli, Chakravarthi ; Panda, Deba Prasad ; Jena, Bikash Ranjan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4592-9229d936d3f8c1275360c2cdafa648186979f5c39fe5a20038d5fb25a6b9a62f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Angiogenesis</topic><topic>Angiogenesis Inhibitors - pharmacology</topic><topic>angiosarcoma</topic><topic>Animals</topic><topic>Cell Proliferation - drug effects</topic><topic>Chick Embryo</topic><topic>Chorioallantoic Membrane</topic><topic>Dephosphorylation</topic><topic>Embryos</topic><topic>Flavonoids</topic><topic>Flavonoids - pharmacology</topic><topic>Gelatinase B</topic><topic>Gene expression</topic><topic>Hemangiosarcoma - drug therapy</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>MMP9</topic><topic>Plant Extracts - pharmacology</topic><topic>Polymerase chain reaction</topic><topic>Signal transduction</topic><topic>Signal Transduction - drug effects</topic><topic>STAT3</topic><topic>Stat3 protein</topic><topic>STAT3 Transcription Factor - metabolism</topic><topic>Tabebuia</topic><topic>Tabebuia chrysantha</topic><topic>TMF</topic><topic>Tumors</topic><topic>Vascular endothelial growth factor</topic><topic>Xenograft Model Antitumor Assays</topic><topic>Xenografts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Panda, Siva Prasad</creatorcontrib><creatorcontrib>Panigrahy, Uttam Prasad</creatorcontrib><creatorcontrib>Prasanth, DSNBK</creatorcontrib><creatorcontrib>Gorla, Uma Sankar</creatorcontrib><creatorcontrib>Guntupalli, Chakravarthi</creatorcontrib><creatorcontrib>Panda, Deba Prasad</creatorcontrib><creatorcontrib>Jena, Bikash Ranjan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Panda, Siva Prasad</au><au>Panigrahy, Uttam Prasad</au><au>Prasanth, DSNBK</au><au>Gorla, Uma Sankar</au><au>Guntupalli, Chakravarthi</au><au>Panda, Deba Prasad</au><au>Jena, Bikash Ranjan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><addtitle>J Pharm Pharmacol</addtitle><date>2020-07</date><risdate>2020</risdate><volume>72</volume><issue>7</issue><spage>990</spage><epage>999</epage><pages>990-999</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><abstract>Objectives
This research aimed to evaluate the antiangiogenic activity of isolated flavonoid 4a,5,8,8a‐tetrahydro‐5‐hydroxy‐3,7,8‐trimethoxy‐2‐(3,4‐dimethoxyphenyl) chromen‐4‐one (TMF) from Tabebuia chrysantha. STAT3‐MMP9 signalling is a signal transduction mechanism that promotes angiogenesis in various cancers.
Methods
The tumour xenografting chicken embryo chorioallantoic membrane (CAM) model‐based ex vivo assay was used to evaluate the activity of TMF. The Western blot, densitometric analysis and quantitative real‐time polymerase chain reaction (qRT‐PCR) were performed to evaluate the activity of the MMP9. Zebrafish embryos were used to evaluate embryotoxicity, and in vitro free radical scavenging activity of flavonoid was also elucidated.
Key findings
This research assessed the high level of STAT3, p‐ERK, VEGF‐R and MMP9 in the tissue extract of the control group, and also, the suppression of angiogenesis in the treatment groups was due to scavenged ROS and RNS, dephosphorylation of STAT3 and ERK, and suppression of MMP9 gene expression.
Conclusion
The isolated flavonoid named TMF from T. chrysantha functions as specific regulators of target proteins of angiosarcoma. The STAT3‐MMP9 signalling may be used as an effective prognostic marker of angiosarcoma.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32311118</pmid><doi>10.1111/jphp.13272</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-6980-6761</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Angiogenesis Angiogenesis Inhibitors - pharmacology angiosarcoma Animals Cell Proliferation - drug effects Chick Embryo Chorioallantoic Membrane Dephosphorylation Embryos Flavonoids Flavonoids - pharmacology Gelatinase B Gene expression Hemangiosarcoma - drug therapy Matrix Metalloproteinase 9 - metabolism MMP9 Plant Extracts - pharmacology Polymerase chain reaction Signal transduction Signal Transduction - drug effects STAT3 Stat3 protein STAT3 Transcription Factor - metabolism Tabebuia Tabebuia chrysantha TMF Tumors Vascular endothelial growth factor Xenograft Model Antitumor Assays Xenografts |
| title | A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma |
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