Recovery of antioxidant and antimicrobial peptides through the reutilization of Nile perch wastewater by biodegradation using two Bacillus species
Nile perch wastewater was biodegraded using two Bacillus species to recover bioactive substances to enhance its reutilization value. The two Bacillus species successfully produced low-molecular-weight substances with a 47.8% degree of hydrolysis. The antioxidant activities of the Nile perch wastewat...
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| Veröffentlicht in: | Chemosphere (Oxford) 2020-08, Vol.253, p.126728-126728, Article 126728 |
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| creator | Mhina, Changoma Fransis Jung, Hyun Yi Kim, Joong Kyun |
| description | Nile perch wastewater was biodegraded using two Bacillus species to recover bioactive substances to enhance its reutilization value. The two Bacillus species successfully produced low-molecular-weight substances with a 47.8% degree of hydrolysis. The antioxidant activities of the Nile perch wastewater increased as the biodegradation proceeded, and the culture supernatant exhibited the highest DPPH (80.1%), ABTS (93.1%) and Fe2+ chelating (88.5%) antioxidant activities at 60 h. The antioxidant potential of the biodegraded Nile perch wastewater was found to be higher than those of other fish hydrolysates. Moreover, the biodegraded Nile perch wastewater exhibited effective antimicrobial activity against Vibrio vulnificus, exhibiting a minimal inhibitory concentration of 585 μg mL−1. Two-dimensional thin layer chromatography analysis revealed the specific amino acids responsible for the antioxidant activity, and molecular-weight cut-off ultrafiltration revealed that the |
| doi_str_mv | 10.1016/j.chemosphere.2020.126728 |
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[Display omitted]
•Bioactive substances are recovered from biodegradation of Nile perch wastewater.•Two Bacillus species are suitable for producing small antioxidant peptides.•The 60-h culture supernatant shows antimicrobial activity against V. vulnificus.•<2-kDa fraction exhibits relatively high antioxidant activity with low IC50 values.•<2-kDa fraction is a good antioxidant and antimicrobial ingredient for nutraceuticals.</description><identifier>ISSN: 0045-6535</identifier><identifier>EISSN: 1879-1298</identifier><identifier>DOI: 10.1016/j.chemosphere.2020.126728</identifier><identifier>PMID: 32298913</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>2-kDa peptides ; Antimicrobial activity ; Antioxidant activity ; Biodegradation ; Nile perch wastewater ; Reutilization value</subject><ispartof>Chemosphere (Oxford), 2020-08, Vol.253, p.126728-126728, Article 126728</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-18b025cfa5abcce91f764689d803f40acdd41f47e57e7310fac339a1e23051d93</citedby><cites>FETCH-LOGICAL-c377t-18b025cfa5abcce91f764689d803f40acdd41f47e57e7310fac339a1e23051d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chemosphere.2020.126728$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32298913$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mhina, Changoma Fransis</creatorcontrib><creatorcontrib>Jung, Hyun Yi</creatorcontrib><creatorcontrib>Kim, Joong Kyun</creatorcontrib><title>Recovery of antioxidant and antimicrobial peptides through the reutilization of Nile perch wastewater by biodegradation using two Bacillus species</title><title>Chemosphere (Oxford)</title><addtitle>Chemosphere</addtitle><description>Nile perch wastewater was biodegraded using two Bacillus species to recover bioactive substances to enhance its reutilization value. The two Bacillus species successfully produced low-molecular-weight substances with a 47.8% degree of hydrolysis. The antioxidant activities of the Nile perch wastewater increased as the biodegradation proceeded, and the culture supernatant exhibited the highest DPPH (80.1%), ABTS (93.1%) and Fe2+ chelating (88.5%) antioxidant activities at 60 h. The antioxidant potential of the biodegraded Nile perch wastewater was found to be higher than those of other fish hydrolysates. Moreover, the biodegraded Nile perch wastewater exhibited effective antimicrobial activity against Vibrio vulnificus, exhibiting a minimal inhibitory concentration of 585 μg mL−1. Two-dimensional thin layer chromatography analysis revealed the specific amino acids responsible for the antioxidant activity, and molecular-weight cut-off ultrafiltration revealed that the <2-kDa fraction exhibited the highest antioxidant activity with the lowest IC50 values (0.43 and 0.22 mg mL−1 for DPPH and ABTS antioxidant activities, respectively). This is the first report of the reutilization of Nile perch wastewater as a natural antioxidant and antimicrobial ingredient for nutraceuticals.
[Display omitted]
•Bioactive substances are recovered from biodegradation of Nile perch wastewater.•Two Bacillus species are suitable for producing small antioxidant peptides.•The 60-h culture supernatant shows antimicrobial activity against V. vulnificus.•<2-kDa fraction exhibits relatively high antioxidant activity with low IC50 values.•<2-kDa fraction is a good antioxidant and antimicrobial ingredient for nutraceuticals.</description><subject>2-kDa peptides</subject><subject>Antimicrobial activity</subject><subject>Antioxidant activity</subject><subject>Biodegradation</subject><subject>Nile perch wastewater</subject><subject>Reutilization value</subject><issn>0045-6535</issn><issn>1879-1298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqNkc9u1DAQxi0EokvhFZC5cclix3EcH2FV_kgVlRCcLceebLxK4mA7XZbH6BPjJQX12NM3Y_2-GXk-hN5QsqWE1u8OW9PD6OPcQ4BtScr8XtaibJ6gDW2ELGgpm6doQ0jFi5ozfoFexHggJJu5fI4uWJkBSdkG3X0D428hnLDvsJ6S87-czZpr-7cfnQm-dXrAM8zJWYg49cEv-z4r4ABLcoP7rbNzOs_46gbIaDA9PuqY4KgTBNyecOu8hX3QdkWX6KY9TkePP2jjhmGJOM5gHMSX6Fmnhwiv7vUS_fh49X33ubi--fRl9_66MEyIVNCmJSU3nea6NQYk7URd1Y20DWFdRbSxtqJdJYALEIySThvGpKZQMsKplewSvV3nzsH_XCAmNbpoYBj0BH6JqmSSSlEJzjIqVzTfIsYAnZqDG3U4KUrUORJ1UA8iUedI1BpJ9r6-X7O0I9j_zn8ZZGC3ApA_e-sgqJjPMBmwLoBJynr3iDV_AIw-p4E</recordid><startdate>202008</startdate><enddate>202008</enddate><creator>Mhina, Changoma Fransis</creator><creator>Jung, Hyun Yi</creator><creator>Kim, Joong Kyun</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202008</creationdate><title>Recovery of antioxidant and antimicrobial peptides through the reutilization of Nile perch wastewater by biodegradation using two Bacillus species</title><author>Mhina, Changoma Fransis ; Jung, Hyun Yi ; Kim, Joong Kyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-18b025cfa5abcce91f764689d803f40acdd41f47e57e7310fac339a1e23051d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>2-kDa peptides</topic><topic>Antimicrobial activity</topic><topic>Antioxidant activity</topic><topic>Biodegradation</topic><topic>Nile perch wastewater</topic><topic>Reutilization value</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mhina, Changoma Fransis</creatorcontrib><creatorcontrib>Jung, Hyun Yi</creatorcontrib><creatorcontrib>Kim, Joong Kyun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chemosphere (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mhina, Changoma Fransis</au><au>Jung, Hyun Yi</au><au>Kim, Joong Kyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recovery of antioxidant and antimicrobial peptides through the reutilization of Nile perch wastewater by biodegradation using two Bacillus species</atitle><jtitle>Chemosphere (Oxford)</jtitle><addtitle>Chemosphere</addtitle><date>2020-08</date><risdate>2020</risdate><volume>253</volume><spage>126728</spage><epage>126728</epage><pages>126728-126728</pages><artnum>126728</artnum><issn>0045-6535</issn><eissn>1879-1298</eissn><abstract>Nile perch wastewater was biodegraded using two Bacillus species to recover bioactive substances to enhance its reutilization value. The two Bacillus species successfully produced low-molecular-weight substances with a 47.8% degree of hydrolysis. The antioxidant activities of the Nile perch wastewater increased as the biodegradation proceeded, and the culture supernatant exhibited the highest DPPH (80.1%), ABTS (93.1%) and Fe2+ chelating (88.5%) antioxidant activities at 60 h. The antioxidant potential of the biodegraded Nile perch wastewater was found to be higher than those of other fish hydrolysates. Moreover, the biodegraded Nile perch wastewater exhibited effective antimicrobial activity against Vibrio vulnificus, exhibiting a minimal inhibitory concentration of 585 μg mL−1. Two-dimensional thin layer chromatography analysis revealed the specific amino acids responsible for the antioxidant activity, and molecular-weight cut-off ultrafiltration revealed that the <2-kDa fraction exhibited the highest antioxidant activity with the lowest IC50 values (0.43 and 0.22 mg mL−1 for DPPH and ABTS antioxidant activities, respectively). This is the first report of the reutilization of Nile perch wastewater as a natural antioxidant and antimicrobial ingredient for nutraceuticals.
[Display omitted]
•Bioactive substances are recovered from biodegradation of Nile perch wastewater.•Two Bacillus species are suitable for producing small antioxidant peptides.•The 60-h culture supernatant shows antimicrobial activity against V. vulnificus.•<2-kDa fraction exhibits relatively high antioxidant activity with low IC50 values.•<2-kDa fraction is a good antioxidant and antimicrobial ingredient for nutraceuticals.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32298913</pmid><doi>10.1016/j.chemosphere.2020.126728</doi><tpages>1</tpages></addata></record> |
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| ispartof | Chemosphere (Oxford), 2020-08, Vol.253, p.126728-126728, Article 126728 |
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| source | Elsevier ScienceDirect Journals |
| subjects | 2-kDa peptides Antimicrobial activity Antioxidant activity Biodegradation Nile perch wastewater Reutilization value |
| title | Recovery of antioxidant and antimicrobial peptides through the reutilization of Nile perch wastewater by biodegradation using two Bacillus species |
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