Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol...

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Veröffentlicht in:Emerging infectious diseases 2020-07, Vol.26 (7), p.1633-1635
Hauptverfasser: Waggoner, Jesse J., Stittleburg, Victoria, Pond, Renee, Saklawi, Youssef, Sahoo, Malaya K., Babiker, Ahmed, Hussaini, Laila, Kraft, Colleen S., Pinsky, Benjamin A., Anderson, Evan J., Rouphael, Nadine
Format: Artikel
Sprache:eng
Schlagworte:
Betacoronavirus - genetics
Betacoronavirus - isolation & purification
Coronaviruses
COVID-19
Disease control
Disease prevention
Expedited
Genes
Humans
Immunology
Infections
Infectious Diseases
Life Sciences & Biomedicine
Medical laboratories
molecular detection
Multiplex Polymerase Chain Reaction - methods
Nasopharynx - virology
Pathogens
Real-Time Polymerase Chain Reaction - methods
Research Letter
respiratory infections
RNA
RNA polymerase
rRT-PCR
SARS-CoV-2
Science & Technology
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Transcription (Genetics)
Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
Viral infections
Viruses
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Beschreibung
Zusammenfassung:Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
ISSN:1080-6040
1080-6059
DOI:10.3201/eid2607.201285