PARP-1 regulates mouse embryonic neural stem cell proliferation by regulating PDGFRα expression
PARP-1 is a multifunctional enzyme that regulates DNA repair, chromatin remodeling, inflammation and cell survival. Our previous study revealed that PARP-1 is required for maintaining normal level of neural stem cell proliferation. In the present study, we present evidence indicating that PARP-1 reg...
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Veröffentlicht in: | Biochemical and biophysical research communications 2020-06, Vol.526 (4), p.986-992 |
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creator | Son, Dae Ik Hong, Seokheon Shin, Ki Soon Kang, Shin Jung |
description | PARP-1 is a multifunctional enzyme that regulates DNA repair, chromatin remodeling, inflammation and cell survival. Our previous study revealed that PARP-1 is required for maintaining normal level of neural stem cell proliferation. In the present study, we present evidence indicating that PARP-1 regulates neural stem cell proliferation by upregulating the expression of platelet-derived growth factor receptor α (PDGFRα). PARP-1 knockout neural stem cells exhibited striking downregulation of PDGFRα expression. We found that PARP-1 promotes the transcription of PDGFRα independently of its enzymatic activity. Overexpression of PDGFRα in the PARP-1 knockout neural stem cells reversed the proliferation defect of the knockout cells. Conversely, knockdown or blocking antibody of PDGFRα suppressed the proliferation of neural stem cells. In addition, blockade of PDGFRα increased cell death rate. Consistent with the downregulation of PDGFRα in the absence of PARP-1, PDGF-AA promoted proliferation of wild-type neural stem cells but not that of PARP-1 knockout cells. These results suggest that PARP-1 can control the neural stem cell proliferation by regulating the expression of PDGFRα.
•PARP-1 promotes the expression of PDGFRα.•PDGFRα downregulation in the PARP-1-deficient neural stem cells impairs proliferation.•PDGFRα regulates the proliferation of neural stem cells. |
doi_str_mv | 10.1016/j.bbrc.2020.03.166 |
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•PARP-1 promotes the expression of PDGFRα.•PDGFRα downregulation in the PARP-1-deficient neural stem cells impairs proliferation.•PDGFRα regulates the proliferation of neural stem cells.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2020.03.166</identifier><identifier>PMID: 32295715</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Neural stem cells ; Neurogenesis ; PARP-1 ; PDGFRα</subject><ispartof>Biochemical and biophysical research communications, 2020-06, Vol.526 (4), p.986-992</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-3c95a75a1b99387c61c7391ee3b012a9868148515fb57db68e8ac3d21f3964613</citedby><cites>FETCH-LOGICAL-c356t-3c95a75a1b99387c61c7391ee3b012a9868148515fb57db68e8ac3d21f3964613</cites><orcidid>0000-0002-3546-7757</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2020.03.166$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32295715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Son, Dae Ik</creatorcontrib><creatorcontrib>Hong, Seokheon</creatorcontrib><creatorcontrib>Shin, Ki Soon</creatorcontrib><creatorcontrib>Kang, Shin Jung</creatorcontrib><title>PARP-1 regulates mouse embryonic neural stem cell proliferation by regulating PDGFRα expression</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>PARP-1 is a multifunctional enzyme that regulates DNA repair, chromatin remodeling, inflammation and cell survival. Our previous study revealed that PARP-1 is required for maintaining normal level of neural stem cell proliferation. In the present study, we present evidence indicating that PARP-1 regulates neural stem cell proliferation by upregulating the expression of platelet-derived growth factor receptor α (PDGFRα). PARP-1 knockout neural stem cells exhibited striking downregulation of PDGFRα expression. We found that PARP-1 promotes the transcription of PDGFRα independently of its enzymatic activity. Overexpression of PDGFRα in the PARP-1 knockout neural stem cells reversed the proliferation defect of the knockout cells. Conversely, knockdown or blocking antibody of PDGFRα suppressed the proliferation of neural stem cells. In addition, blockade of PDGFRα increased cell death rate. Consistent with the downregulation of PDGFRα in the absence of PARP-1, PDGF-AA promoted proliferation of wild-type neural stem cells but not that of PARP-1 knockout cells. These results suggest that PARP-1 can control the neural stem cell proliferation by regulating the expression of PDGFRα.
•PARP-1 promotes the expression of PDGFRα.•PDGFRα downregulation in the PARP-1-deficient neural stem cells impairs proliferation.•PDGFRα regulates the proliferation of neural stem cells.</description><subject>Neural stem cells</subject><subject>Neurogenesis</subject><subject>PARP-1</subject><subject>PDGFRα</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kM1u1DAUhS1URIeWF-ii8rKbhHvtxImlbqqWFqRKjCqQujO2c1N5lJ-pnSDmsXgRnomMpmXJ6m6-c3Tux9gZQo6A6uMmdy76XICAHGSOSr1hKwQNmUAojtgKAFQmND4es_cpbQAQC6XfsWMphC4rLFfsx_rqYZ0hj_Q0d3aixPtxTsSpd3E3DsHzgeZoO54m6rmnruPbOHahpWinMA7c7V6zYXji65u724c_vzn92kZKaQFO2dvWdok-vNwT9v3207frz9n917sv11f3mZelmjLpdWmr0qLTWtaVV-grqZFIOkBhda1qLOoSy9aVVeNUTbX1shHYSq0KhfKEXRx6l3nPM6XJ9CHt99qBlo-MkBpUUUhQCyoOqI9jSpFas42ht3FnEMzerNmYvVmzN2tAmsXsEjp_6Z9dT82_yKvKBbg8ALR8-TNQNMkHGjw1IZKfTDOG__X_BQo4ih0</recordid><startdate>20200611</startdate><enddate>20200611</enddate><creator>Son, Dae Ik</creator><creator>Hong, Seokheon</creator><creator>Shin, Ki Soon</creator><creator>Kang, Shin Jung</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3546-7757</orcidid></search><sort><creationdate>20200611</creationdate><title>PARP-1 regulates mouse embryonic neural stem cell proliferation by regulating PDGFRα expression</title><author>Son, Dae Ik ; Hong, Seokheon ; Shin, Ki Soon ; Kang, Shin Jung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-3c95a75a1b99387c61c7391ee3b012a9868148515fb57db68e8ac3d21f3964613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Neural stem cells</topic><topic>Neurogenesis</topic><topic>PARP-1</topic><topic>PDGFRα</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Son, Dae Ik</creatorcontrib><creatorcontrib>Hong, Seokheon</creatorcontrib><creatorcontrib>Shin, Ki Soon</creatorcontrib><creatorcontrib>Kang, Shin Jung</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Son, Dae Ik</au><au>Hong, Seokheon</au><au>Shin, Ki Soon</au><au>Kang, Shin Jung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PARP-1 regulates mouse embryonic neural stem cell proliferation by regulating PDGFRα expression</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2020-06-11</date><risdate>2020</risdate><volume>526</volume><issue>4</issue><spage>986</spage><epage>992</epage><pages>986-992</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>PARP-1 is a multifunctional enzyme that regulates DNA repair, chromatin remodeling, inflammation and cell survival. Our previous study revealed that PARP-1 is required for maintaining normal level of neural stem cell proliferation. In the present study, we present evidence indicating that PARP-1 regulates neural stem cell proliferation by upregulating the expression of platelet-derived growth factor receptor α (PDGFRα). PARP-1 knockout neural stem cells exhibited striking downregulation of PDGFRα expression. We found that PARP-1 promotes the transcription of PDGFRα independently of its enzymatic activity. Overexpression of PDGFRα in the PARP-1 knockout neural stem cells reversed the proliferation defect of the knockout cells. Conversely, knockdown or blocking antibody of PDGFRα suppressed the proliferation of neural stem cells. In addition, blockade of PDGFRα increased cell death rate. Consistent with the downregulation of PDGFRα in the absence of PARP-1, PDGF-AA promoted proliferation of wild-type neural stem cells but not that of PARP-1 knockout cells. These results suggest that PARP-1 can control the neural stem cell proliferation by regulating the expression of PDGFRα.
•PARP-1 promotes the expression of PDGFRα.•PDGFRα downregulation in the PARP-1-deficient neural stem cells impairs proliferation.•PDGFRα regulates the proliferation of neural stem cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>32295715</pmid><doi>10.1016/j.bbrc.2020.03.166</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-3546-7757</orcidid></addata></record> |
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subjects | Neural stem cells Neurogenesis PARP-1 PDGFRα |
title | PARP-1 regulates mouse embryonic neural stem cell proliferation by regulating PDGFRα expression |
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