Detection of miR-122 by fluorescence real-time PCR in blood from patients with chronic hepatitis B and C infections
This study aims to determine whether relative miR-122 levels in peripheral blood are correlated with chronic hepatitis B (CHB) and chronic hepatitis C (CHC) virus infection and viral replication to determine whether miR-122 can be a new marker for liver injury. MicroRNA (miRNA) was extracted from th...
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| Veröffentlicht in: | Cytokine (Philadelphia, Pa.) Pa.), 2020-07, Vol.131, p.155076-155076, Article 155076 |
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| container_title | Cytokine (Philadelphia, Pa.) |
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| creator | Ma, Zhen-Hua Sun, Cheng-Xue Shi, Hong Fan, Jian-Hua Song, Yu-Guo Cong, Pei-Jun Kong, Xiang-Min Hao, Da-Lin |
| description | This study aims to determine whether relative miR-122 levels in peripheral blood are correlated with chronic hepatitis B (CHB) and chronic hepatitis C (CHC) virus infection and viral replication to determine whether miR-122 can be a new marker for liver injury.
MicroRNA (miRNA) was extracted from the peripheral blood of 20 CHB patients, 20 CHC patients, and 20 healthy controls. The levels of miR-122 were determined using fluorescence real-time reverse transcription PCR. Then, the associations of miR-122 with CHB and CHC were analyzed, and its correlation with other markers of liver function and viral replication were determined.
The expression level of miR-122 in patients with CHB was significantly higher when compared to subjects in the control group (P = 0.007) or CHC patients (P = 0.005). Furthermore, the miR-122 level in patients with CHC was somewhat higher when compared to healthy controls (66% higher), but the difference was not statistically significant (P = 0.229). MiR-122 levels were significantly correlated with ALT (correlation coefficient [R] = 0.7, P |
| doi_str_mv | 10.1016/j.cyto.2020.155076 |
| format | Article |
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MicroRNA (miRNA) was extracted from the peripheral blood of 20 CHB patients, 20 CHC patients, and 20 healthy controls. The levels of miR-122 were determined using fluorescence real-time reverse transcription PCR. Then, the associations of miR-122 with CHB and CHC were analyzed, and its correlation with other markers of liver function and viral replication were determined.
The expression level of miR-122 in patients with CHB was significantly higher when compared to subjects in the control group (P = 0.007) or CHC patients (P = 0.005). Furthermore, the miR-122 level in patients with CHC was somewhat higher when compared to healthy controls (66% higher), but the difference was not statistically significant (P = 0.229). MiR-122 levels were significantly correlated with ALT (correlation coefficient [R] = 0.7, P < 0.001), AST (R = 0.71, P < 0.001), and HBV NA (R = 0.9, P < 0.001). The regression analysis indicated that the AUC of miR-122 levels in the diagnosis of CHB was 0.87, with a sensitivity of 0.8 and a specificity of 0.8.
MiR-122 can be used to distinguish healthy persons and patients with CHB infection with high sensitivity and specificity. These present findings presented that the complex and context-specific associations of miR-122 with liver diseases, suggesting that this may be a promising marker for liver injury.</description><identifier>ISSN: 1043-4666</identifier><identifier>EISSN: 1096-0023</identifier><identifier>DOI: 10.1016/j.cyto.2020.155076</identifier><identifier>PMID: 32289629</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Chronic hepatitis B ; Chronic hepatitis C ; Fluorescence real-time PCR ; miR-122</subject><ispartof>Cytokine (Philadelphia, Pa.), 2020-07, Vol.131, p.155076-155076, Article 155076</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-e82a721827d43f2717656510c0a74141aef98f147e16498b58eeb0979e0203373</citedby><cites>FETCH-LOGICAL-c356t-e82a721827d43f2717656510c0a74141aef98f147e16498b58eeb0979e0203373</cites><orcidid>0000-0003-0484-0421</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cyto.2020.155076$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32289629$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Zhen-Hua</creatorcontrib><creatorcontrib>Sun, Cheng-Xue</creatorcontrib><creatorcontrib>Shi, Hong</creatorcontrib><creatorcontrib>Fan, Jian-Hua</creatorcontrib><creatorcontrib>Song, Yu-Guo</creatorcontrib><creatorcontrib>Cong, Pei-Jun</creatorcontrib><creatorcontrib>Kong, Xiang-Min</creatorcontrib><creatorcontrib>Hao, Da-Lin</creatorcontrib><title>Detection of miR-122 by fluorescence real-time PCR in blood from patients with chronic hepatitis B and C infections</title><title>Cytokine (Philadelphia, Pa.)</title><addtitle>Cytokine</addtitle><description>This study aims to determine whether relative miR-122 levels in peripheral blood are correlated with chronic hepatitis B (CHB) and chronic hepatitis C (CHC) virus infection and viral replication to determine whether miR-122 can be a new marker for liver injury.
MicroRNA (miRNA) was extracted from the peripheral blood of 20 CHB patients, 20 CHC patients, and 20 healthy controls. The levels of miR-122 were determined using fluorescence real-time reverse transcription PCR. Then, the associations of miR-122 with CHB and CHC were analyzed, and its correlation with other markers of liver function and viral replication were determined.
The expression level of miR-122 in patients with CHB was significantly higher when compared to subjects in the control group (P = 0.007) or CHC patients (P = 0.005). Furthermore, the miR-122 level in patients with CHC was somewhat higher when compared to healthy controls (66% higher), but the difference was not statistically significant (P = 0.229). MiR-122 levels were significantly correlated with ALT (correlation coefficient [R] = 0.7, P < 0.001), AST (R = 0.71, P < 0.001), and HBV NA (R = 0.9, P < 0.001). The regression analysis indicated that the AUC of miR-122 levels in the diagnosis of CHB was 0.87, with a sensitivity of 0.8 and a specificity of 0.8.
MiR-122 can be used to distinguish healthy persons and patients with CHB infection with high sensitivity and specificity. These present findings presented that the complex and context-specific associations of miR-122 with liver diseases, suggesting that this may be a promising marker for liver injury.</description><subject>Chronic hepatitis B</subject><subject>Chronic hepatitis C</subject><subject>Fluorescence real-time PCR</subject><subject>miR-122</subject><issn>1043-4666</issn><issn>1096-0023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kEtr3DAUhUVpyKv5A10ELbvxRA9bsiCbdpoXDCSEdi1k-YrRYFtTSZMy_74yTrvs6l4u55zL-RD6TMmKEipudit7zGHFCCuHpiFSfEDnlChREcL4x3mveVULIc7QRUo7QojiUp6iM85YqwRT5yh9hww2-zDh4PDoXyvKGO6O2A2HECFZmCzgCGaosh8Bv6xfsZ9wN4TQYxfDiPcme5hywr993mK7jWHyFm9hvmef8Ddsph6vi8stj9IndOLMkODqfV6in_d3P9aP1eb54Wn9dVNZ3ohcQcuMZLRlsq-5Y5JK0YiGEkuMrGlNDTjVOlpLoKJWbde0AB1RUkEBwrnkl-jLkruP4dcBUtajL4WGwUwQDkkzrgitVcNokbJFamNIKYLT--hHE4-aEj3D1js9w9YzbL3ALqbr9_xDN0L_z_KXbhHcLgIoLd88RJ2sn4H2PhYWug_-f_l_APnkjno</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Ma, Zhen-Hua</creator><creator>Sun, Cheng-Xue</creator><creator>Shi, Hong</creator><creator>Fan, Jian-Hua</creator><creator>Song, Yu-Guo</creator><creator>Cong, Pei-Jun</creator><creator>Kong, Xiang-Min</creator><creator>Hao, Da-Lin</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0484-0421</orcidid></search><sort><creationdate>202007</creationdate><title>Detection of miR-122 by fluorescence real-time PCR in blood from patients with chronic hepatitis B and C infections</title><author>Ma, Zhen-Hua ; Sun, Cheng-Xue ; Shi, Hong ; Fan, Jian-Hua ; Song, Yu-Guo ; Cong, Pei-Jun ; Kong, Xiang-Min ; Hao, Da-Lin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-e82a721827d43f2717656510c0a74141aef98f147e16498b58eeb0979e0203373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Chronic hepatitis B</topic><topic>Chronic hepatitis C</topic><topic>Fluorescence real-time PCR</topic><topic>miR-122</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Zhen-Hua</creatorcontrib><creatorcontrib>Sun, Cheng-Xue</creatorcontrib><creatorcontrib>Shi, Hong</creatorcontrib><creatorcontrib>Fan, Jian-Hua</creatorcontrib><creatorcontrib>Song, Yu-Guo</creatorcontrib><creatorcontrib>Cong, Pei-Jun</creatorcontrib><creatorcontrib>Kong, Xiang-Min</creatorcontrib><creatorcontrib>Hao, Da-Lin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytokine (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Zhen-Hua</au><au>Sun, Cheng-Xue</au><au>Shi, Hong</au><au>Fan, Jian-Hua</au><au>Song, Yu-Guo</au><au>Cong, Pei-Jun</au><au>Kong, Xiang-Min</au><au>Hao, Da-Lin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of miR-122 by fluorescence real-time PCR in blood from patients with chronic hepatitis B and C infections</atitle><jtitle>Cytokine (Philadelphia, Pa.)</jtitle><addtitle>Cytokine</addtitle><date>2020-07</date><risdate>2020</risdate><volume>131</volume><spage>155076</spage><epage>155076</epage><pages>155076-155076</pages><artnum>155076</artnum><issn>1043-4666</issn><eissn>1096-0023</eissn><abstract>This study aims to determine whether relative miR-122 levels in peripheral blood are correlated with chronic hepatitis B (CHB) and chronic hepatitis C (CHC) virus infection and viral replication to determine whether miR-122 can be a new marker for liver injury.
MicroRNA (miRNA) was extracted from the peripheral blood of 20 CHB patients, 20 CHC patients, and 20 healthy controls. The levels of miR-122 were determined using fluorescence real-time reverse transcription PCR. Then, the associations of miR-122 with CHB and CHC were analyzed, and its correlation with other markers of liver function and viral replication were determined.
The expression level of miR-122 in patients with CHB was significantly higher when compared to subjects in the control group (P = 0.007) or CHC patients (P = 0.005). Furthermore, the miR-122 level in patients with CHC was somewhat higher when compared to healthy controls (66% higher), but the difference was not statistically significant (P = 0.229). MiR-122 levels were significantly correlated with ALT (correlation coefficient [R] = 0.7, P < 0.001), AST (R = 0.71, P < 0.001), and HBV NA (R = 0.9, P < 0.001). The regression analysis indicated that the AUC of miR-122 levels in the diagnosis of CHB was 0.87, with a sensitivity of 0.8 and a specificity of 0.8.
MiR-122 can be used to distinguish healthy persons and patients with CHB infection with high sensitivity and specificity. These present findings presented that the complex and context-specific associations of miR-122 with liver diseases, suggesting that this may be a promising marker for liver injury.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32289629</pmid><doi>10.1016/j.cyto.2020.155076</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-0484-0421</orcidid></addata></record> |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Chronic hepatitis B Chronic hepatitis C Fluorescence real-time PCR miR-122 |
| title | Detection of miR-122 by fluorescence real-time PCR in blood from patients with chronic hepatitis B and C infections |
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