Colorimetric Chemosensor and Turn on Fluorescence Probe for pH Monitoring Based on Xanthene Dye Derivatives and its Bioimaging of Living Escherichia coli Bacteria
A new turn on fluorescence probe based on 3′,6′-dihydroxy-6-methyl-2-((pyridin-2-ylmethylene)amino)-4-( p -tolyl)spiro[benzo[f]isoindole-1,9′-xanthen]-3(2H)-one ( BFFPH ) derived from benzo[f]fluorescein was prepared. Full characterization of the prepared probe using spectroscopic analysis was descr...
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| Veröffentlicht in: | Journal of fluorescence 2020-05, Vol.30 (3), p.601-612 |
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| creator | Mohamed, Mahmoud Basseem I. Aysha, Tarek S. Elmorsi, Taha M. El-Sedik, Mervat Omara, Shimaa T. Shaban, Elkhabiry Kandil, Omaima M. Bedair, Ahmed H. |
| description | A new turn on fluorescence probe based on 3′,6′-dihydroxy-6-methyl-2-((pyridin-2-ylmethylene)amino)-4-(
p
-tolyl)spiro[benzo[f]isoindole-1,9′-xanthen]-3(2H)-one (
BFFPH
) derived from benzo[f]fluorescein was prepared. Full characterization of the prepared probe using spectroscopic analysis was described such as IR, NMR and MS spectra. The sensitivity of
BFFPH
for monitoring of pH change in alkaline medium was studied.
BFFPH
exhibited a high sensitivity to alkaline pH by two pKa values at 8.82 and 10.66 in UV/vis spectroscopy titration. The pH monitoring was studied in broad range of pH values (2.5–12.2) at two pKa values at 8.72 and 10.73 by recording the effect of pH on the fluorescence intensity of
BFFPH
. The acid-base reversibility character of the probe was investigated as well as the effect of the pH change on the fluorescence quantum yield. The application of the prepared
BFFPH
probe for detection of living
Escherichia coli (E. coli
) bacteria using confocal fluorescence microscope was investigated. |
| doi_str_mv | 10.1007/s10895-020-02522-1 |
| format | Article |
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p
-tolyl)spiro[benzo[f]isoindole-1,9′-xanthen]-3(2H)-one (
BFFPH
) derived from benzo[f]fluorescein was prepared. Full characterization of the prepared probe using spectroscopic analysis was described such as IR, NMR and MS spectra. The sensitivity of
BFFPH
for monitoring of pH change in alkaline medium was studied.
BFFPH
exhibited a high sensitivity to alkaline pH by two pKa values at 8.82 and 10.66 in UV/vis spectroscopy titration. The pH monitoring was studied in broad range of pH values (2.5–12.2) at two pKa values at 8.72 and 10.73 by recording the effect of pH on the fluorescence intensity of
BFFPH
. The acid-base reversibility character of the probe was investigated as well as the effect of the pH change on the fluorescence quantum yield. The application of the prepared
BFFPH
probe for detection of living
Escherichia coli (E. coli
) bacteria using confocal fluorescence microscope was investigated.</description><identifier>ISSN: 1053-0509</identifier><identifier>EISSN: 1573-4994</identifier><identifier>DOI: 10.1007/s10895-020-02522-1</identifier><identifier>PMID: 32285236</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analytical Chemistry ; Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biomedicine ; Biophysics ; Biosensing Techniques ; Biotechnology ; Chemical sensors ; Chemoreceptors ; Coliforms ; Colorimetry ; E coli ; Escherichia coli - isolation & purification ; Fluorescein ; Fluorescent Dyes - chemical synthesis ; Fluorescent Dyes - chemistry ; Fluorescent indicators ; Hydrogen-Ion Concentration ; Medical imaging ; Molecular Structure ; Monitoring ; NMR ; Nuclear magnetic resonance ; Optical Imaging ; Original Article ; Spectral sensitivity ; Spectrum analysis ; Titration ; Xanthenes - chemical synthesis ; Xanthenes - chemistry</subject><ispartof>Journal of fluorescence, 2020-05, Vol.30 (3), p.601-612</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-b19bfabc4a9c5c2e84c874effeace833728170daa8d2dcb265f88116b5bb79c73</citedby><cites>FETCH-LOGICAL-c375t-b19bfabc4a9c5c2e84c874effeace833728170daa8d2dcb265f88116b5bb79c73</cites><orcidid>0000-0001-5558-6342</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10895-020-02522-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10895-020-02522-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32285236$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mohamed, Mahmoud Basseem I.</creatorcontrib><creatorcontrib>Aysha, Tarek S.</creatorcontrib><creatorcontrib>Elmorsi, Taha M.</creatorcontrib><creatorcontrib>El-Sedik, Mervat</creatorcontrib><creatorcontrib>Omara, Shimaa T.</creatorcontrib><creatorcontrib>Shaban, Elkhabiry</creatorcontrib><creatorcontrib>Kandil, Omaima M.</creatorcontrib><creatorcontrib>Bedair, Ahmed H.</creatorcontrib><title>Colorimetric Chemosensor and Turn on Fluorescence Probe for pH Monitoring Based on Xanthene Dye Derivatives and its Bioimaging of Living Escherichia coli Bacteria</title><title>Journal of fluorescence</title><addtitle>J Fluoresc</addtitle><addtitle>J Fluoresc</addtitle><description>A new turn on fluorescence probe based on 3′,6′-dihydroxy-6-methyl-2-((pyridin-2-ylmethylene)amino)-4-(
p
-tolyl)spiro[benzo[f]isoindole-1,9′-xanthen]-3(2H)-one (
BFFPH
) derived from benzo[f]fluorescein was prepared. Full characterization of the prepared probe using spectroscopic analysis was described such as IR, NMR and MS spectra. The sensitivity of
BFFPH
for monitoring of pH change in alkaline medium was studied.
BFFPH
exhibited a high sensitivity to alkaline pH by two pKa values at 8.82 and 10.66 in UV/vis spectroscopy titration. The pH monitoring was studied in broad range of pH values (2.5–12.2) at two pKa values at 8.72 and 10.73 by recording the effect of pH on the fluorescence intensity of
BFFPH
. The acid-base reversibility character of the probe was investigated as well as the effect of the pH change on the fluorescence quantum yield. The application of the prepared
BFFPH
probe for detection of living
Escherichia coli (E. coli
) bacteria using confocal fluorescence microscope was investigated.</description><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biophysics</subject><subject>Biosensing Techniques</subject><subject>Biotechnology</subject><subject>Chemical sensors</subject><subject>Chemoreceptors</subject><subject>Coliforms</subject><subject>Colorimetry</subject><subject>E coli</subject><subject>Escherichia coli - isolation & purification</subject><subject>Fluorescein</subject><subject>Fluorescent Dyes - chemical synthesis</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent indicators</subject><subject>Hydrogen-Ion Concentration</subject><subject>Medical imaging</subject><subject>Molecular Structure</subject><subject>Monitoring</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Optical Imaging</subject><subject>Original Article</subject><subject>Spectral sensitivity</subject><subject>Spectrum analysis</subject><subject>Titration</subject><subject>Xanthenes - chemical synthesis</subject><subject>Xanthenes - chemistry</subject><issn>1053-0509</issn><issn>1573-4994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhS0EoqXwAiyQJTZsAv5N7CUdWoo0CBZFYmfZzk3HVWIPdjJSX4cnxekUkFiwuPKV9d1z7XMQeknJW0pI965QorRsCCO1JGMNfYROqex4I7QWj2tPJG-IJPoEPSvllhCilVBP0QlnTEnG21P0c5PGlMMEcw4eb3YwpQKxpIxt7PH1kiNOEV-OS8pQPEQP-GtODvBQkf0V_pximKtAvMHntkC_0t9tnHcQAX-4qwU5HOwcDlDuJcNc8HlIYbI361Aa8DYc1u6i-F1l_S5Y7NMYqp6f64V9jp4Mdizw4uE8Q98uL643V832y8dPm_fbxvNOzo2j2g3WeWG1l56BEl51AoYBrAfFeccU7UhvrepZ7x1r5aAUpa2TznXad_wMvTnq7nP6sUCZzRTql8fRRkhLMYwr3Woh-Iq-_ge9TdWq-jrDBGl1KzRpK8WOlM-plAyD2Venbb4zlJg1QXNM0NQEzX2ChtahVw_Si5ug_zPyO7IK8CNQ9qvtkP_u_o_sL-WWqQM</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Mohamed, Mahmoud Basseem I.</creator><creator>Aysha, Tarek S.</creator><creator>Elmorsi, Taha M.</creator><creator>El-Sedik, Mervat</creator><creator>Omara, Shimaa T.</creator><creator>Shaban, Elkhabiry</creator><creator>Kandil, Omaima M.</creator><creator>Bedair, Ahmed H.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5558-6342</orcidid></search><sort><creationdate>20200501</creationdate><title>Colorimetric Chemosensor and Turn on Fluorescence Probe for pH Monitoring Based on Xanthene Dye Derivatives and its Bioimaging of Living Escherichia coli Bacteria</title><author>Mohamed, Mahmoud Basseem I. ; Aysha, Tarek S. ; Elmorsi, Taha M. ; El-Sedik, Mervat ; Omara, Shimaa T. ; Shaban, Elkhabiry ; Kandil, Omaima M. ; Bedair, Ahmed H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-b19bfabc4a9c5c2e84c874effeace833728170daa8d2dcb265f88116b5bb79c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analytical Chemistry</topic><topic>Biochemistry</topic><topic>Biological and Medical Physics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biophysics</topic><topic>Biosensing Techniques</topic><topic>Biotechnology</topic><topic>Chemical sensors</topic><topic>Chemoreceptors</topic><topic>Coliforms</topic><topic>Colorimetry</topic><topic>E coli</topic><topic>Escherichia coli - isolation & purification</topic><topic>Fluorescein</topic><topic>Fluorescent Dyes - chemical synthesis</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent indicators</topic><topic>Hydrogen-Ion Concentration</topic><topic>Medical imaging</topic><topic>Molecular Structure</topic><topic>Monitoring</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Optical Imaging</topic><topic>Original Article</topic><topic>Spectral sensitivity</topic><topic>Spectrum analysis</topic><topic>Titration</topic><topic>Xanthenes - chemical synthesis</topic><topic>Xanthenes - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohamed, Mahmoud Basseem I.</creatorcontrib><creatorcontrib>Aysha, Tarek S.</creatorcontrib><creatorcontrib>Elmorsi, Taha M.</creatorcontrib><creatorcontrib>El-Sedik, Mervat</creatorcontrib><creatorcontrib>Omara, Shimaa T.</creatorcontrib><creatorcontrib>Shaban, Elkhabiry</creatorcontrib><creatorcontrib>Kandil, Omaima M.</creatorcontrib><creatorcontrib>Bedair, Ahmed H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of fluorescence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohamed, Mahmoud Basseem I.</au><au>Aysha, Tarek S.</au><au>Elmorsi, Taha M.</au><au>El-Sedik, Mervat</au><au>Omara, Shimaa T.</au><au>Shaban, Elkhabiry</au><au>Kandil, Omaima M.</au><au>Bedair, Ahmed H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Colorimetric Chemosensor and Turn on Fluorescence Probe for pH Monitoring Based on Xanthene Dye Derivatives and its Bioimaging of Living Escherichia coli Bacteria</atitle><jtitle>Journal of fluorescence</jtitle><stitle>J Fluoresc</stitle><addtitle>J Fluoresc</addtitle><date>2020-05-01</date><risdate>2020</risdate><volume>30</volume><issue>3</issue><spage>601</spage><epage>612</epage><pages>601-612</pages><issn>1053-0509</issn><eissn>1573-4994</eissn><abstract>A new turn on fluorescence probe based on 3′,6′-dihydroxy-6-methyl-2-((pyridin-2-ylmethylene)amino)-4-(
p
-tolyl)spiro[benzo[f]isoindole-1,9′-xanthen]-3(2H)-one (
BFFPH
) derived from benzo[f]fluorescein was prepared. Full characterization of the prepared probe using spectroscopic analysis was described such as IR, NMR and MS spectra. The sensitivity of
BFFPH
for monitoring of pH change in alkaline medium was studied.
BFFPH
exhibited a high sensitivity to alkaline pH by two pKa values at 8.82 and 10.66 in UV/vis spectroscopy titration. The pH monitoring was studied in broad range of pH values (2.5–12.2) at two pKa values at 8.72 and 10.73 by recording the effect of pH on the fluorescence intensity of
BFFPH
. The acid-base reversibility character of the probe was investigated as well as the effect of the pH change on the fluorescence quantum yield. The application of the prepared
BFFPH
probe for detection of living
Escherichia coli (E. coli
) bacteria using confocal fluorescence microscope was investigated.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32285236</pmid><doi>10.1007/s10895-020-02522-1</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-5558-6342</orcidid></addata></record> |
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| ispartof | Journal of fluorescence, 2020-05, Vol.30 (3), p.601-612 |
| issn | 1053-0509 1573-4994 |
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| subjects | Analytical Chemistry Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biomedicine Biophysics Biosensing Techniques Biotechnology Chemical sensors Chemoreceptors Coliforms Colorimetry E coli Escherichia coli - isolation & purification Fluorescein Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Fluorescent indicators Hydrogen-Ion Concentration Medical imaging Molecular Structure Monitoring NMR Nuclear magnetic resonance Optical Imaging Original Article Spectral sensitivity Spectrum analysis Titration Xanthenes - chemical synthesis Xanthenes - chemistry |
| title | Colorimetric Chemosensor and Turn on Fluorescence Probe for pH Monitoring Based on Xanthene Dye Derivatives and its Bioimaging of Living Escherichia coli Bacteria |
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