Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis
[Abstract] Porphyromonas gingivalis is a keystone pathogen and major colonizer in host tissue which plays a pivotal role in periodontitis among the other polymicrobial infections. Increasing facts demonstrate that curcumin has antibacterial activity and anti-biofilm effect against the periodontopath...
Gespeichert in:
| Veröffentlicht in: | Odontology 2021-01, Vol.109 (1), p.18-28 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 28 |
|---|---|
| container_issue | 1 |
| container_start_page | 18 |
| container_title | Odontology |
| container_volume | 109 |
| creator | Kumbar, Vijay M. Peram, Malleswara Rao Kugaji, Manohar S. Shah, Tejas Patil, Sanjivani P. Muddapur, Uday M. Bhat, Kishore G. |
| description | [Abstract] Porphyromonas gingivalis is a keystone pathogen and major colonizer in host tissue which plays a pivotal role in periodontitis among the other polymicrobial infections. Increasing facts demonstrate that curcumin has antibacterial activity and anti-biofilm effect against the periodontopathogens through diverse mechanisms that have a positive impact on periodontal health. The present study was aimed to elucidate the effect of curcumin on biofilm formation and virulence factor gene expression of P. gingivalis. By using gene expression studies, we exploited the mechanism of anti-biofilm effects of curcumin on P. gingivalis. The minimum inhibitory concentration and minimum bactericidal concentration of curcumin for both ATCC and clinical strains of P. gingivalis were found to be 62.5 and 125 μg ml-1 respectively. Curcumin prevented bacterial adhesion and biofilm formation in a dose-dependent manner. Further, curcumin attenuated the virulence of P. gingivalis by reducing the expression of genes coding for major virulence factors, including adhesions (fimA, hagA, and hagB) and proteinases (rgpA, rgpB, and kgp). The results indicated that curcumin has shown anti-biofilm as well as antibacterial activity against P. gingivalis. Further, curcumin because of its pleiotropic actions could be a simple and inexpensive therapeutic strategy in the treatment of periodontal disease. |
| doi_str_mv | 10.1007/s10266-020-00514-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2389209442</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2389209442</sourcerecordid><originalsourceid>FETCH-LOGICAL-c463t-9172cc8296dc4c0e1bf718e53e51be4b149459396bf39fef5c56722b8da73e8a3</originalsourceid><addsrcrecordid>eNp9kc1u1TAQhSMEolXpC7BAltiwIODxT2IvUVUKUiVYwNpynHFqlNgXOynct8eQ0kosWNgz0nznjOXTNM-BvgFK-7cFKOu6ljLaUipBtMdHzSl0oFpgUj6-70V_0pyXEgYqGADtQT5tTjhjvWZMnzaHS-_RrSR54rbstiVEkiKZcvqx3rwmQ0g-zAvxKS92DXVi40huQ95mjA6Jt25NmUwYkeDPQ8a6qULV7XPKh5tjTkuKtpApxCnc2jmUZ80Tb-eC53f1rPn6_vLLxYf2-tPVx4t3160THV9bDT1zTjHdjU44ijD4HhRKjhIGFAMILaTmuhs81x69dLLrGRvUaHuOyvKz5tXue8jp-4ZlNUsoDufZRkxbMYwrzagWglX05T_ot7TlWF9n6vd1IHuuVKXYTrmcSsnozSGHxeajAWp-R2L2SEyNxPyJxByr6MWd9TYsON5L_gZQAb4DpY7ihPlh939tr3ZV9QzOzinOIeKD1E2QxhRXwygDQ4FqWgvl9YCqF1NciQ6E4L8AFZKuhw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2476157388</pqid></control><display><type>article</type><title>Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Kumbar, Vijay M. ; Peram, Malleswara Rao ; Kugaji, Manohar S. ; Shah, Tejas ; Patil, Sanjivani P. ; Muddapur, Uday M. ; Bhat, Kishore G.</creator><creatorcontrib>Kumbar, Vijay M. ; Peram, Malleswara Rao ; Kugaji, Manohar S. ; Shah, Tejas ; Patil, Sanjivani P. ; Muddapur, Uday M. ; Bhat, Kishore G. ; Department of Pharmaceutics ; Central Research Laboratory ; Maratha Mandal's College of Pharmacy ; Sinhgad College of Engineering ; Department of Biotechnology ; KLE Technological University (Formerly Known as B.V.Bhoomaraddi College of Engineering and Technology ; BVB Campus ; Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre ; Affiliated to Savitribai Phule Pune University</creatorcontrib><description>[Abstract] Porphyromonas gingivalis is a keystone pathogen and major colonizer in host tissue which plays a pivotal role in periodontitis among the other polymicrobial infections. Increasing facts demonstrate that curcumin has antibacterial activity and anti-biofilm effect against the periodontopathogens through diverse mechanisms that have a positive impact on periodontal health. The present study was aimed to elucidate the effect of curcumin on biofilm formation and virulence factor gene expression of P. gingivalis. By using gene expression studies, we exploited the mechanism of anti-biofilm effects of curcumin on P. gingivalis. The minimum inhibitory concentration and minimum bactericidal concentration of curcumin for both ATCC and clinical strains of P. gingivalis were found to be 62.5 and 125 μg ml-1 respectively. Curcumin prevented bacterial adhesion and biofilm formation in a dose-dependent manner. Further, curcumin attenuated the virulence of P. gingivalis by reducing the expression of genes coding for major virulence factors, including adhesions (fimA, hagA, and hagB) and proteinases (rgpA, rgpB, and kgp). The results indicated that curcumin has shown anti-biofilm as well as antibacterial activity against P. gingivalis. Further, curcumin because of its pleiotropic actions could be a simple and inexpensive therapeutic strategy in the treatment of periodontal disease.</description><identifier>ISSN: 1618-1247</identifier><identifier>EISSN: 1618-1255</identifier><identifier>DOI: 10.1007/s10266-020-00514-y</identifier><identifier>PMID: 32279229</identifier><language>eng</language><publisher>Singapore: The Society of the Nippon Dental University</publisher><subject>Adhesins, Bacterial - genetics ; Antibacterial activity ; Biofilms ; Curcumin ; Curcumin - pharmacology ; Dentistry ; Gene Expression ; Medicine ; Minimum inhibitory concentration ; Oral and Maxillofacial Surgery ; Original Article ; Periodontal diseases ; Periodontitis ; Porphyromonas gingivalis ; Virulence ; Virulence factors ; Virulence Factors - genetics</subject><ispartof>Odontology, 2021-01, Vol.109 (1), p.18-28</ispartof><rights>The Society of The Nippon Dental University 2020</rights><rights>The Society of The Nippon Dental University 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-9172cc8296dc4c0e1bf718e53e51be4b149459396bf39fef5c56722b8da73e8a3</citedby><cites>FETCH-LOGICAL-c463t-9172cc8296dc4c0e1bf718e53e51be4b149459396bf39fef5c56722b8da73e8a3</cites><orcidid>0000-0002-9563-2531 ; 0000-0002-1984-6538 ; 0000-0001-6315-4531 ; 0000-0001-6261-1665</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10266-020-00514-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10266-020-00514-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32279229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumbar, Vijay M.</creatorcontrib><creatorcontrib>Peram, Malleswara Rao</creatorcontrib><creatorcontrib>Kugaji, Manohar S.</creatorcontrib><creatorcontrib>Shah, Tejas</creatorcontrib><creatorcontrib>Patil, Sanjivani P.</creatorcontrib><creatorcontrib>Muddapur, Uday M.</creatorcontrib><creatorcontrib>Bhat, Kishore G.</creatorcontrib><creatorcontrib>Department of Pharmaceutics</creatorcontrib><creatorcontrib>Central Research Laboratory</creatorcontrib><creatorcontrib>Maratha Mandal's College of Pharmacy</creatorcontrib><creatorcontrib>Sinhgad College of Engineering</creatorcontrib><creatorcontrib>Department of Biotechnology</creatorcontrib><creatorcontrib>KLE Technological University (Formerly Known as B.V.Bhoomaraddi College of Engineering and Technology</creatorcontrib><creatorcontrib>BVB Campus</creatorcontrib><creatorcontrib>Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre</creatorcontrib><creatorcontrib>Affiliated to Savitribai Phule Pune University</creatorcontrib><title>Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis</title><title>Odontology</title><addtitle>Odontology</addtitle><addtitle>Odontology</addtitle><description>[Abstract] Porphyromonas gingivalis is a keystone pathogen and major colonizer in host tissue which plays a pivotal role in periodontitis among the other polymicrobial infections. Increasing facts demonstrate that curcumin has antibacterial activity and anti-biofilm effect against the periodontopathogens through diverse mechanisms that have a positive impact on periodontal health. The present study was aimed to elucidate the effect of curcumin on biofilm formation and virulence factor gene expression of P. gingivalis. By using gene expression studies, we exploited the mechanism of anti-biofilm effects of curcumin on P. gingivalis. The minimum inhibitory concentration and minimum bactericidal concentration of curcumin for both ATCC and clinical strains of P. gingivalis were found to be 62.5 and 125 μg ml-1 respectively. Curcumin prevented bacterial adhesion and biofilm formation in a dose-dependent manner. Further, curcumin attenuated the virulence of P. gingivalis by reducing the expression of genes coding for major virulence factors, including adhesions (fimA, hagA, and hagB) and proteinases (rgpA, rgpB, and kgp). The results indicated that curcumin has shown anti-biofilm as well as antibacterial activity against P. gingivalis. Further, curcumin because of its pleiotropic actions could be a simple and inexpensive therapeutic strategy in the treatment of periodontal disease.</description><subject>Adhesins, Bacterial - genetics</subject><subject>Antibacterial activity</subject><subject>Biofilms</subject><subject>Curcumin</subject><subject>Curcumin - pharmacology</subject><subject>Dentistry</subject><subject>Gene Expression</subject><subject>Medicine</subject><subject>Minimum inhibitory concentration</subject><subject>Oral and Maxillofacial Surgery</subject><subject>Original Article</subject><subject>Periodontal diseases</subject><subject>Periodontitis</subject><subject>Porphyromonas gingivalis</subject><subject>Virulence</subject><subject>Virulence factors</subject><subject>Virulence Factors - genetics</subject><issn>1618-1247</issn><issn>1618-1255</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1TAQhSMEolXpC7BAltiwIODxT2IvUVUKUiVYwNpynHFqlNgXOynct8eQ0kosWNgz0nznjOXTNM-BvgFK-7cFKOu6ljLaUipBtMdHzSl0oFpgUj6-70V_0pyXEgYqGADtQT5tTjhjvWZMnzaHS-_RrSR54rbstiVEkiKZcvqx3rwmQ0g-zAvxKS92DXVi40huQ95mjA6Jt25NmUwYkeDPQ8a6qULV7XPKh5tjTkuKtpApxCnc2jmUZ80Tb-eC53f1rPn6_vLLxYf2-tPVx4t3160THV9bDT1zTjHdjU44ijD4HhRKjhIGFAMILaTmuhs81x69dLLrGRvUaHuOyvKz5tXue8jp-4ZlNUsoDufZRkxbMYwrzagWglX05T_ot7TlWF9n6vd1IHuuVKXYTrmcSsnozSGHxeajAWp-R2L2SEyNxPyJxByr6MWd9TYsON5L_gZQAb4DpY7ihPlh939tr3ZV9QzOzinOIeKD1E2QxhRXwygDQ4FqWgvl9YCqF1NciQ6E4L8AFZKuhw</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Kumbar, Vijay M.</creator><creator>Peram, Malleswara Rao</creator><creator>Kugaji, Manohar S.</creator><creator>Shah, Tejas</creator><creator>Patil, Sanjivani P.</creator><creator>Muddapur, Uday M.</creator><creator>Bhat, Kishore G.</creator><general>The Society of the Nippon Dental University</general><general>Springer Singapore</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9563-2531</orcidid><orcidid>https://orcid.org/0000-0002-1984-6538</orcidid><orcidid>https://orcid.org/0000-0001-6315-4531</orcidid><orcidid>https://orcid.org/0000-0001-6261-1665</orcidid></search><sort><creationdate>20210101</creationdate><title>Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis</title><author>Kumbar, Vijay M. ; Peram, Malleswara Rao ; Kugaji, Manohar S. ; Shah, Tejas ; Patil, Sanjivani P. ; Muddapur, Uday M. ; Bhat, Kishore G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-9172cc8296dc4c0e1bf718e53e51be4b149459396bf39fef5c56722b8da73e8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adhesins, Bacterial - genetics</topic><topic>Antibacterial activity</topic><topic>Biofilms</topic><topic>Curcumin</topic><topic>Curcumin - pharmacology</topic><topic>Dentistry</topic><topic>Gene Expression</topic><topic>Medicine</topic><topic>Minimum inhibitory concentration</topic><topic>Oral and Maxillofacial Surgery</topic><topic>Original Article</topic><topic>Periodontal diseases</topic><topic>Periodontitis</topic><topic>Porphyromonas gingivalis</topic><topic>Virulence</topic><topic>Virulence factors</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumbar, Vijay M.</creatorcontrib><creatorcontrib>Peram, Malleswara Rao</creatorcontrib><creatorcontrib>Kugaji, Manohar S.</creatorcontrib><creatorcontrib>Shah, Tejas</creatorcontrib><creatorcontrib>Patil, Sanjivani P.</creatorcontrib><creatorcontrib>Muddapur, Uday M.</creatorcontrib><creatorcontrib>Bhat, Kishore G.</creatorcontrib><creatorcontrib>Department of Pharmaceutics</creatorcontrib><creatorcontrib>Central Research Laboratory</creatorcontrib><creatorcontrib>Maratha Mandal's College of Pharmacy</creatorcontrib><creatorcontrib>Sinhgad College of Engineering</creatorcontrib><creatorcontrib>Department of Biotechnology</creatorcontrib><creatorcontrib>KLE Technological University (Formerly Known as B.V.Bhoomaraddi College of Engineering and Technology</creatorcontrib><creatorcontrib>BVB Campus</creatorcontrib><creatorcontrib>Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre</creatorcontrib><creatorcontrib>Affiliated to Savitribai Phule Pune University</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Odontology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumbar, Vijay M.</au><au>Peram, Malleswara Rao</au><au>Kugaji, Manohar S.</au><au>Shah, Tejas</au><au>Patil, Sanjivani P.</au><au>Muddapur, Uday M.</au><au>Bhat, Kishore G.</au><aucorp>Department of Pharmaceutics</aucorp><aucorp>Central Research Laboratory</aucorp><aucorp>Maratha Mandal's College of Pharmacy</aucorp><aucorp>Sinhgad College of Engineering</aucorp><aucorp>Department of Biotechnology</aucorp><aucorp>KLE Technological University (Formerly Known as B.V.Bhoomaraddi College of Engineering and Technology</aucorp><aucorp>BVB Campus</aucorp><aucorp>Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre</aucorp><aucorp>Affiliated to Savitribai Phule Pune University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis</atitle><jtitle>Odontology</jtitle><stitle>Odontology</stitle><addtitle>Odontology</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>109</volume><issue>1</issue><spage>18</spage><epage>28</epage><pages>18-28</pages><issn>1618-1247</issn><eissn>1618-1255</eissn><abstract>[Abstract] Porphyromonas gingivalis is a keystone pathogen and major colonizer in host tissue which plays a pivotal role in periodontitis among the other polymicrobial infections. Increasing facts demonstrate that curcumin has antibacterial activity and anti-biofilm effect against the periodontopathogens through diverse mechanisms that have a positive impact on periodontal health. The present study was aimed to elucidate the effect of curcumin on biofilm formation and virulence factor gene expression of P. gingivalis. By using gene expression studies, we exploited the mechanism of anti-biofilm effects of curcumin on P. gingivalis. The minimum inhibitory concentration and minimum bactericidal concentration of curcumin for both ATCC and clinical strains of P. gingivalis were found to be 62.5 and 125 μg ml-1 respectively. Curcumin prevented bacterial adhesion and biofilm formation in a dose-dependent manner. Further, curcumin attenuated the virulence of P. gingivalis by reducing the expression of genes coding for major virulence factors, including adhesions (fimA, hagA, and hagB) and proteinases (rgpA, rgpB, and kgp). The results indicated that curcumin has shown anti-biofilm as well as antibacterial activity against P. gingivalis. Further, curcumin because of its pleiotropic actions could be a simple and inexpensive therapeutic strategy in the treatment of periodontal disease.</abstract><cop>Singapore</cop><pub>The Society of the Nippon Dental University</pub><pmid>32279229</pmid><doi>10.1007/s10266-020-00514-y</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-9563-2531</orcidid><orcidid>https://orcid.org/0000-0002-1984-6538</orcidid><orcidid>https://orcid.org/0000-0001-6315-4531</orcidid><orcidid>https://orcid.org/0000-0001-6261-1665</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-1247 |
| ispartof | Odontology, 2021-01, Vol.109 (1), p.18-28 |
| issn | 1618-1247 1618-1255 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2389209442 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adhesins, Bacterial - genetics Antibacterial activity Biofilms Curcumin Curcumin - pharmacology Dentistry Gene Expression Medicine Minimum inhibitory concentration Oral and Maxillofacial Surgery Original Article Periodontal diseases Periodontitis Porphyromonas gingivalis Virulence Virulence factors Virulence Factors - genetics |
| title | Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T23%3A14%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20curcumin%20on%20growth,%20biofilm%20formation%20and%20virulence%20factor%20gene%20expression%20of%20Porphyromonas%20gingivalis&rft.jtitle=Odontology&rft.au=Kumbar,%20Vijay%20M.&rft.aucorp=Department%20of%20Pharmaceutics&rft.date=2021-01-01&rft.volume=109&rft.issue=1&rft.spage=18&rft.epage=28&rft.pages=18-28&rft.issn=1618-1247&rft.eissn=1618-1255&rft_id=info:doi/10.1007/s10266-020-00514-y&rft_dat=%3Cproquest_cross%3E2389209442%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2476157388&rft_id=info:pmid/32279229&rfr_iscdi=true |