Production of added-value microbial metabolites during growth of yeast strains on media composed of biodiesel-derived crude glycerol and glycerol/xylose blends
ABSTRACT A total of 11 yeast strains of Yarrowia lipolytica, Metschnikowia sp., Rhodotorula sp. and Rhodosporidium toruloides were grown under nitrogen-limited conditions with crude glycerol employed as substrate in shake flasks, presenting interesting dry cell weight (DCW) production. Three of thes...
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description | ABSTRACT
A total of 11 yeast strains of Yarrowia lipolytica, Metschnikowia sp., Rhodotorula sp. and Rhodosporidium toruloides were grown under nitrogen-limited conditions with crude glycerol employed as substrate in shake flasks, presenting interesting dry cell weight (DCW) production. Three of these strains belonging to Metschnikowia sp. accumulated significant quantities of endopolysaccharides (i.e. the strain V.V.-D4 produced 11.0 g/L of endopolysaccharides, with polysaccharides in DCW ≈ 63% w/w). A total of six Y. lipolytica strains produced either citric acid or mannitol. Most of the screened yeasts presented somehow elevated lipid and polysaccharides in DCW values at the early steps of growth despite nitrogen appearance in the fermentation medium. Lipid in DCW values decreased as growth proceeded. R. toruloides DSM 4444 cultivated on media presenting higher glycerol concentrations presented interesting lipid-accumulating capacities (maximum lipid = 12.5 g/L, maximum lipid in DCW = 43.0–46.0% w/w, conversion yield on glycerol = 0.16 g/g). Replacement of crude glycerol by xylose resulted in somehow decreased lipid accumulation. In xylose/glycerol mixtures, xylose was more rapidly assimilated from glycerol. R. toruloides total lipids were mainly composed of triacylglycerols. Total cellular fatty acid composition on xylose presented some differences compared with that on glycerol. Cellular lipids contained mainly oleic and palmitic acid.
Value-added metabolites synthesized by fermentation of glycerol and glycerol-xylose blends by yeasts. |
doi_str_mv | 10.1093/femsle/fnaa063 |
format | Article |
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A total of 11 yeast strains of Yarrowia lipolytica, Metschnikowia sp., Rhodotorula sp. and Rhodosporidium toruloides were grown under nitrogen-limited conditions with crude glycerol employed as substrate in shake flasks, presenting interesting dry cell weight (DCW) production. Three of these strains belonging to Metschnikowia sp. accumulated significant quantities of endopolysaccharides (i.e. the strain V.V.-D4 produced 11.0 g/L of endopolysaccharides, with polysaccharides in DCW ≈ 63% w/w). A total of six Y. lipolytica strains produced either citric acid or mannitol. Most of the screened yeasts presented somehow elevated lipid and polysaccharides in DCW values at the early steps of growth despite nitrogen appearance in the fermentation medium. Lipid in DCW values decreased as growth proceeded. R. toruloides DSM 4444 cultivated on media presenting higher glycerol concentrations presented interesting lipid-accumulating capacities (maximum lipid = 12.5 g/L, maximum lipid in DCW = 43.0–46.0% w/w, conversion yield on glycerol = 0.16 g/g). Replacement of crude glycerol by xylose resulted in somehow decreased lipid accumulation. In xylose/glycerol mixtures, xylose was more rapidly assimilated from glycerol. R. toruloides total lipids were mainly composed of triacylglycerols. Total cellular fatty acid composition on xylose presented some differences compared with that on glycerol. Cellular lipids contained mainly oleic and palmitic acid.
Value-added metabolites synthesized by fermentation of glycerol and glycerol-xylose blends by yeasts.</description><identifier>ISSN: 1574-6968</identifier><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1093/femsle/fnaa063</identifier><identifier>PMID: 32275306</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Accumulation ; Biodiesel fuels ; Biofuels ; Cell culture ; Citric acid ; Culture media ; Culture Media - chemistry ; Culture Media - metabolism ; Dry cells ; Fatty acid composition ; Fatty acids ; Fermentation ; Flasks ; Glycerin ; Glycerol ; Glycerol - metabolism ; Industrial Microbiology - methods ; Lipids ; Mannitol ; Metabolites ; Metschnikowia ; Microbiology ; Microorganisms ; Monosaccharides ; Nitrogen ; Palmitic acid ; Physiological aspects ; Polysaccharides ; Saccharides ; Substrates ; Sugars ; Triglycerides ; Xylose ; Xylose - metabolism ; Yeast ; Yeast fungi ; Yeasts ; Yeasts - growth & development ; Yeasts - metabolism</subject><ispartof>FEMS microbiology letters, 2020-05, Vol.367 (10), p.1</ispartof><rights>The Author(s) 2019. Published by Oxford University Press on behalf of FEMS. 2020</rights><rights>FEMS 2020.</rights><rights>COPYRIGHT 2020 Oxford University Press</rights><rights>The Author(s) 2019. Published by Oxford University Press on behalf of FEMS.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-5e2b56538bce400777303f963968a03231fb9423affd4765dcdcf913d2813d4c3</citedby><cites>FETCH-LOGICAL-c458t-5e2b56538bce400777303f963968a03231fb9423affd4765dcdcf913d2813d4c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1583,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32275306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Diamantopoulou, Panagiota</creatorcontrib><creatorcontrib>Filippousi, Rosanina</creatorcontrib><creatorcontrib>Antoniou, Dimitrios</creatorcontrib><creatorcontrib>Varfi, Evaggelia</creatorcontrib><creatorcontrib>Xenopoulos, Evangelos</creatorcontrib><creatorcontrib>Sarris, Dimitris</creatorcontrib><creatorcontrib>Papanikolaou, Seraphim</creatorcontrib><title>Production of added-value microbial metabolites during growth of yeast strains on media composed of biodiesel-derived crude glycerol and glycerol/xylose blends</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>ABSTRACT
A total of 11 yeast strains of Yarrowia lipolytica, Metschnikowia sp., Rhodotorula sp. and Rhodosporidium toruloides were grown under nitrogen-limited conditions with crude glycerol employed as substrate in shake flasks, presenting interesting dry cell weight (DCW) production. Three of these strains belonging to Metschnikowia sp. accumulated significant quantities of endopolysaccharides (i.e. the strain V.V.-D4 produced 11.0 g/L of endopolysaccharides, with polysaccharides in DCW ≈ 63% w/w). A total of six Y. lipolytica strains produced either citric acid or mannitol. Most of the screened yeasts presented somehow elevated lipid and polysaccharides in DCW values at the early steps of growth despite nitrogen appearance in the fermentation medium. Lipid in DCW values decreased as growth proceeded. R. toruloides DSM 4444 cultivated on media presenting higher glycerol concentrations presented interesting lipid-accumulating capacities (maximum lipid = 12.5 g/L, maximum lipid in DCW = 43.0–46.0% w/w, conversion yield on glycerol = 0.16 g/g). Replacement of crude glycerol by xylose resulted in somehow decreased lipid accumulation. In xylose/glycerol mixtures, xylose was more rapidly assimilated from glycerol. R. toruloides total lipids were mainly composed of triacylglycerols. Total cellular fatty acid composition on xylose presented some differences compared with that on glycerol. Cellular lipids contained mainly oleic and palmitic acid.
Value-added metabolites synthesized by fermentation of glycerol and glycerol-xylose blends by yeasts.</description><subject>Accumulation</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Cell culture</subject><subject>Citric acid</subject><subject>Culture media</subject><subject>Culture Media - chemistry</subject><subject>Culture Media - metabolism</subject><subject>Dry cells</subject><subject>Fatty acid composition</subject><subject>Fatty acids</subject><subject>Fermentation</subject><subject>Flasks</subject><subject>Glycerin</subject><subject>Glycerol</subject><subject>Glycerol - metabolism</subject><subject>Industrial Microbiology - methods</subject><subject>Lipids</subject><subject>Mannitol</subject><subject>Metabolites</subject><subject>Metschnikowia</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Monosaccharides</subject><subject>Nitrogen</subject><subject>Palmitic acid</subject><subject>Physiological aspects</subject><subject>Polysaccharides</subject><subject>Saccharides</subject><subject>Substrates</subject><subject>Sugars</subject><subject>Triglycerides</subject><subject>Xylose</subject><subject>Xylose - metabolism</subject><subject>Yeast</subject><subject>Yeast fungi</subject><subject>Yeasts</subject><subject>Yeasts - growth & development</subject><subject>Yeasts - metabolism</subject><issn>1574-6968</issn><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFUttqFTEUHUSxtfrqowR80YfpySSTzMxjKV4KBcXLc8gkO8eUTHLMpe35Gn_VHHpsrRQkkMtmrbX3zl5N87LDxx2e6MrAkhysjJcSc_qoOezY0Ld84uPjv-4HzbOULjDGPcH8aXNACRkYxfyw-fU5Bl1UtsGjYJDUGnR7KV0BtFgVw2ylQwtkOQdnMySkS7R-jdYxXOUfO8oWZMoo5SitT6jKLKCtRCosm5BA7yCzDdpCAtdqiPayBlUsGtDabRXE4JD0-vaxut66SkSzA6_T8-aJkS7Bi_151Hx__-7b6cf2_NOHs9OT81b1bMwtAzIzzug4K-gxHoaBYmomTmvzElNCOzNPPaHSGN0PnGmllZk6qslYt17Ro-bNje4mhp8FUhaLTQqckx5CSYLQcRzJyBmp0Nf_QC9Cib5WJwjDZKKsJrhDraUDYb0J9YfUTlSc8KlWSDtOK-r4AVRdGur3Bw_G1vg9wtt7hIrJcJ3XsqQkzr5-eVC8zjGlCEZsol1k3IoOi517xI17xN49lfBq31mZ6xRv4X_scpc9lM3_xH4DvJXRGQ</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Diamantopoulou, Panagiota</creator><creator>Filippousi, Rosanina</creator><creator>Antoniou, Dimitrios</creator><creator>Varfi, Evaggelia</creator><creator>Xenopoulos, Evangelos</creator><creator>Sarris, Dimitris</creator><creator>Papanikolaou, Seraphim</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20200501</creationdate><title>Production of added-value microbial metabolites during growth of yeast strains on media composed of biodiesel-derived crude glycerol and glycerol/xylose blends</title><author>Diamantopoulou, Panagiota ; Filippousi, Rosanina ; Antoniou, Dimitrios ; Varfi, Evaggelia ; Xenopoulos, Evangelos ; Sarris, Dimitris ; Papanikolaou, Seraphim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-5e2b56538bce400777303f963968a03231fb9423affd4765dcdcf913d2813d4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Accumulation</topic><topic>Biodiesel fuels</topic><topic>Biofuels</topic><topic>Cell culture</topic><topic>Citric acid</topic><topic>Culture media</topic><topic>Culture Media - chemistry</topic><topic>Culture Media - metabolism</topic><topic>Dry cells</topic><topic>Fatty acid composition</topic><topic>Fatty acids</topic><topic>Fermentation</topic><topic>Flasks</topic><topic>Glycerin</topic><topic>Glycerol</topic><topic>Glycerol - metabolism</topic><topic>Industrial Microbiology - methods</topic><topic>Lipids</topic><topic>Mannitol</topic><topic>Metabolites</topic><topic>Metschnikowia</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Monosaccharides</topic><topic>Nitrogen</topic><topic>Palmitic acid</topic><topic>Physiological aspects</topic><topic>Polysaccharides</topic><topic>Saccharides</topic><topic>Substrates</topic><topic>Sugars</topic><topic>Triglycerides</topic><topic>Xylose</topic><topic>Xylose - metabolism</topic><topic>Yeast</topic><topic>Yeast fungi</topic><topic>Yeasts</topic><topic>Yeasts - growth & development</topic><topic>Yeasts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Diamantopoulou, Panagiota</creatorcontrib><creatorcontrib>Filippousi, Rosanina</creatorcontrib><creatorcontrib>Antoniou, Dimitrios</creatorcontrib><creatorcontrib>Varfi, Evaggelia</creatorcontrib><creatorcontrib>Xenopoulos, Evangelos</creatorcontrib><creatorcontrib>Sarris, Dimitris</creatorcontrib><creatorcontrib>Papanikolaou, Seraphim</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Diamantopoulou, Panagiota</au><au>Filippousi, Rosanina</au><au>Antoniou, Dimitrios</au><au>Varfi, Evaggelia</au><au>Xenopoulos, Evangelos</au><au>Sarris, Dimitris</au><au>Papanikolaou, Seraphim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of added-value microbial metabolites during growth of yeast strains on media composed of biodiesel-derived crude glycerol and glycerol/xylose blends</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2020-05-01</date><risdate>2020</risdate><volume>367</volume><issue>10</issue><spage>1</spage><pages>1-</pages><issn>1574-6968</issn><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>ABSTRACT
A total of 11 yeast strains of Yarrowia lipolytica, Metschnikowia sp., Rhodotorula sp. and Rhodosporidium toruloides were grown under nitrogen-limited conditions with crude glycerol employed as substrate in shake flasks, presenting interesting dry cell weight (DCW) production. Three of these strains belonging to Metschnikowia sp. accumulated significant quantities of endopolysaccharides (i.e. the strain V.V.-D4 produced 11.0 g/L of endopolysaccharides, with polysaccharides in DCW ≈ 63% w/w). A total of six Y. lipolytica strains produced either citric acid or mannitol. Most of the screened yeasts presented somehow elevated lipid and polysaccharides in DCW values at the early steps of growth despite nitrogen appearance in the fermentation medium. Lipid in DCW values decreased as growth proceeded. R. toruloides DSM 4444 cultivated on media presenting higher glycerol concentrations presented interesting lipid-accumulating capacities (maximum lipid = 12.5 g/L, maximum lipid in DCW = 43.0–46.0% w/w, conversion yield on glycerol = 0.16 g/g). Replacement of crude glycerol by xylose resulted in somehow decreased lipid accumulation. In xylose/glycerol mixtures, xylose was more rapidly assimilated from glycerol. R. toruloides total lipids were mainly composed of triacylglycerols. Total cellular fatty acid composition on xylose presented some differences compared with that on glycerol. Cellular lipids contained mainly oleic and palmitic acid.
Value-added metabolites synthesized by fermentation of glycerol and glycerol-xylose blends by yeasts.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>32275306</pmid><doi>10.1093/femsle/fnaa063</doi><tpages>13</tpages></addata></record> |
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subjects | Accumulation Biodiesel fuels Biofuels Cell culture Citric acid Culture media Culture Media - chemistry Culture Media - metabolism Dry cells Fatty acid composition Fatty acids Fermentation Flasks Glycerin Glycerol Glycerol - metabolism Industrial Microbiology - methods Lipids Mannitol Metabolites Metschnikowia Microbiology Microorganisms Monosaccharides Nitrogen Palmitic acid Physiological aspects Polysaccharides Saccharides Substrates Sugars Triglycerides Xylose Xylose - metabolism Yeast Yeast fungi Yeasts Yeasts - growth & development Yeasts - metabolism |
title | Production of added-value microbial metabolites during growth of yeast strains on media composed of biodiesel-derived crude glycerol and glycerol/xylose blends |
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