Production of added-value microbial metabolites during growth of yeast strains on media composed of biodiesel-derived crude glycerol and glycerol/xylose blends

ABSTRACT A total of 11 yeast strains of Yarrowia lipolytica, Metschnikowia sp., Rhodotorula sp. and Rhodosporidium toruloides were grown under nitrogen-limited conditions with crude glycerol employed as substrate in shake flasks, presenting interesting dry cell weight (DCW) production. Three of thes...

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Veröffentlicht in:FEMS microbiology letters 2020-05, Vol.367 (10), p.1
Hauptverfasser: Diamantopoulou, Panagiota, Filippousi, Rosanina, Antoniou, Dimitrios, Varfi, Evaggelia, Xenopoulos, Evangelos, Sarris, Dimitris, Papanikolaou, Seraphim
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container_issue 10
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container_title FEMS microbiology letters
container_volume 367
creator Diamantopoulou, Panagiota
Filippousi, Rosanina
Antoniou, Dimitrios
Varfi, Evaggelia
Xenopoulos, Evangelos
Sarris, Dimitris
Papanikolaou, Seraphim
description ABSTRACT A total of 11 yeast strains of Yarrowia lipolytica, Metschnikowia sp., Rhodotorula sp. and Rhodosporidium toruloides were grown under nitrogen-limited conditions with crude glycerol employed as substrate in shake flasks, presenting interesting dry cell weight (DCW) production. Three of these strains belonging to Metschnikowia sp. accumulated significant quantities of endopolysaccharides (i.e. the strain V.V.-D4 produced 11.0 g/L of endopolysaccharides, with polysaccharides in DCW ≈ 63% w/w). A total of six Y. lipolytica strains produced either citric acid or mannitol. Most of the screened yeasts presented somehow elevated lipid and polysaccharides in DCW values at the early steps of growth despite nitrogen appearance in the fermentation medium. Lipid in DCW values decreased as growth proceeded. R. toruloides DSM 4444 cultivated on media presenting higher glycerol concentrations presented interesting lipid-accumulating capacities (maximum lipid = 12.5 g/L, maximum lipid in DCW = 43.0–46.0% w/w, conversion yield on glycerol = 0.16 g/g). Replacement of crude glycerol by xylose resulted in somehow decreased lipid accumulation. In xylose/glycerol mixtures, xylose was more rapidly assimilated from glycerol. R. toruloides total lipids were mainly composed of triacylglycerols. Total cellular fatty acid composition on xylose presented some differences compared with that on glycerol. Cellular lipids contained mainly oleic and palmitic acid. Value-added metabolites synthesized by fermentation of glycerol and glycerol-xylose blends by yeasts.
doi_str_mv 10.1093/femsle/fnaa063
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Three of these strains belonging to Metschnikowia sp. accumulated significant quantities of endopolysaccharides (i.e. the strain V.V.-D4 produced 11.0 g/L of endopolysaccharides, with polysaccharides in DCW ≈ 63% w/w). A total of six Y. lipolytica strains produced either citric acid or mannitol. Most of the screened yeasts presented somehow elevated lipid and polysaccharides in DCW values at the early steps of growth despite nitrogen appearance in the fermentation medium. Lipid in DCW values decreased as growth proceeded. R. toruloides DSM 4444 cultivated on media presenting higher glycerol concentrations presented interesting lipid-accumulating capacities (maximum lipid = 12.5 g/L, maximum lipid in DCW = 43.0–46.0% w/w, conversion yield on glycerol = 0.16 g/g). Replacement of crude glycerol by xylose resulted in somehow decreased lipid accumulation. In xylose/glycerol mixtures, xylose was more rapidly assimilated from glycerol. R. toruloides total lipids were mainly composed of triacylglycerols. Total cellular fatty acid composition on xylose presented some differences compared with that on glycerol. Cellular lipids contained mainly oleic and palmitic acid. 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subjects Accumulation
Biodiesel fuels
Biofuels
Cell culture
Citric acid
Culture media
Culture Media - chemistry
Culture Media - metabolism
Dry cells
Fatty acid composition
Fatty acids
Fermentation
Flasks
Glycerin
Glycerol
Glycerol - metabolism
Industrial Microbiology - methods
Lipids
Mannitol
Metabolites
Metschnikowia
Microbiology
Microorganisms
Monosaccharides
Nitrogen
Palmitic acid
Physiological aspects
Polysaccharides
Saccharides
Substrates
Sugars
Triglycerides
Xylose
Xylose - metabolism
Yeast
Yeast fungi
Yeasts
Yeasts - growth & development
Yeasts - metabolism
title Production of added-value microbial metabolites during growth of yeast strains on media composed of biodiesel-derived crude glycerol and glycerol/xylose blends
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