Novel lactone‐layered double hydroxide ionomer powders for bone tissue repair
This article describes the use of a novel lactone‐layered double hydroxide polymer network (PN), derived from a poly(lactide‐co‐caprolactone) copolymer, as a controlled ion‐release agent for artificial bone tissue regeneration. The osteogenic cell culture Saos‐2 is used as a test culture to investig...
Gespeichert in:
| Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2020-10, Vol.108 (7), p.2835-2846 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2846 |
|---|---|
| container_issue | 7 |
| container_start_page | 2835 |
| container_title | Journal of biomedical materials research. Part B, Applied biomaterials |
| container_volume | 108 |
| creator | Zhou, Tianhao McCarthy, Edward D. Soutis, Constantinos Cartmell, Sarah H. |
| description | This article describes the use of a novel lactone‐layered double hydroxide polymer network (PN), derived from a poly(lactide‐co‐caprolactone) copolymer, as a controlled ion‐release agent for artificial bone tissue regeneration. The osteogenic cell culture Saos‐2 is used as a test culture to investigate the PN's performance as an extracellular ion‐release agent. The compelling performance of this PN is demonstrated in both growth and osteogenic media compared with a control of cells grown on tissue culture plastic (TCP) without PN. Firstly, the PNs released concentration of magnesium ions over time ranging from 10 to 60 mM after 24 hr, depending on the PN sample. After incubation of Saos‐2 with the PN, while no difference was seen in cell number, there was significant upregulation of bone‐related gene expression at 14 days—~5fold increase in Bone Morphogenetic Protein 2, ~3fold increase in osteopontin and ~2fold increase in collagen Type I. In addition, normalized alkaline phosphatase activity was seen to significantly increase by ~2fold with PN presence. A ~4fold increase in collagen Type I protein expression (via Gomori Trichrome Stain) was observed with PN presence. In addition, a ~4fold increase in phosphate deposits (as seen with Von Kossa staining analysis) was seen with PN presence. It is found that this novel PN material has a significant potential for bone tissue regeneration. |
| doi_str_mv | 10.1002/jbm.b.34614 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2388819227</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2388819227</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4344-4dc1b8299c6b59db172cad80c46c78b643f81be9767e3884f9a58d42aba93e8a3</originalsourceid><addsrcrecordid>eNp90LlOAzEQBmALgUg4KnpkiQYJJayP3bVLEnGKo4Ha8jErNtqNg50lpOMReEaeBEOAgoLKU3zza_wjtEeyIckyejwx7dAMGS8IX0N9kud0wKUg679zyXpoK8ZJwkWWs03UY5SWZS5lH93d-mdocKPt3E_h_fWt0UsI4LDznWkAPy5d8C-1A1z7qW8h4JlfOAgRVz5gk3bwvI6xAxxgpuuwgzYq3UTY_X630cPZ6f34YnB9d345PrkeWM44H3BniRFUSluYXDpDSmq1E5nlhS2FKTirBDEgy6IEJgSvpM6F41QbLRkIzbbR4Sp3FvxTB3Gu2jpaaBo9Bd9FRdOWIDL9M9GDP3TiuzBN1ynKEytlkYukjlbKBh9jgErNQt3qsFQkU589q9SzMuqr56T3vzM704L7tT_FJkBXYFE3sPwvS12Nbkar1A9Q4ImH</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2438879658</pqid></control><display><type>article</type><title>Novel lactone‐layered double hydroxide ionomer powders for bone tissue repair</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Zhou, Tianhao ; McCarthy, Edward D. ; Soutis, Constantinos ; Cartmell, Sarah H.</creator><creatorcontrib>Zhou, Tianhao ; McCarthy, Edward D. ; Soutis, Constantinos ; Cartmell, Sarah H.</creatorcontrib><description>This article describes the use of a novel lactone‐layered double hydroxide polymer network (PN), derived from a poly(lactide‐co‐caprolactone) copolymer, as a controlled ion‐release agent for artificial bone tissue regeneration. The osteogenic cell culture Saos‐2 is used as a test culture to investigate the PN's performance as an extracellular ion‐release agent. The compelling performance of this PN is demonstrated in both growth and osteogenic media compared with a control of cells grown on tissue culture plastic (TCP) without PN. Firstly, the PNs released concentration of magnesium ions over time ranging from 10 to 60 mM after 24 hr, depending on the PN sample. After incubation of Saos‐2 with the PN, while no difference was seen in cell number, there was significant upregulation of bone‐related gene expression at 14 days—~5fold increase in Bone Morphogenetic Protein 2, ~3fold increase in osteopontin and ~2fold increase in collagen Type I. In addition, normalized alkaline phosphatase activity was seen to significantly increase by ~2fold with PN presence. A ~4fold increase in collagen Type I protein expression (via Gomori Trichrome Stain) was observed with PN presence. In addition, a ~4fold increase in phosphate deposits (as seen with Von Kossa staining analysis) was seen with PN presence. It is found that this novel PN material has a significant potential for bone tissue regeneration.</description><identifier>ISSN: 1552-4973</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.34614</identifier><identifier>PMID: 32277599</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Alkaline phosphatase ; Biomedical materials ; Bone growth ; Bone morphogenetic protein 2 ; bone repair ; Bones ; Cell culture ; Cell number ; Collagen ; Collagen (type I) ; Copolymers ; Gene expression ; Ionomers ; layered double hydroxide ; Magnesium ; Materials research ; Materials science ; Osteopontin ; polycaprolactone ; polylactic acid ; Polymers ; Proteins ; Regeneration ; Release agents ; SAOS2 ; Tissue culture ; Tissue engineering</subject><ispartof>Journal of biomedical materials research. Part B, Applied biomaterials, 2020-10, Vol.108 (7), p.2835-2846</ispartof><rights>2020 The Authors. published by Wiley Periodicals, Inc.</rights><rights>2020 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc.</rights><rights>2020. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4344-4dc1b8299c6b59db172cad80c46c78b643f81be9767e3884f9a58d42aba93e8a3</citedby><cites>FETCH-LOGICAL-c4344-4dc1b8299c6b59db172cad80c46c78b643f81be9767e3884f9a58d42aba93e8a3</cites><orcidid>0000-0002-2038-2929</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.b.34614$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.b.34614$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32277599$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Tianhao</creatorcontrib><creatorcontrib>McCarthy, Edward D.</creatorcontrib><creatorcontrib>Soutis, Constantinos</creatorcontrib><creatorcontrib>Cartmell, Sarah H.</creatorcontrib><title>Novel lactone‐layered double hydroxide ionomer powders for bone tissue repair</title><title>Journal of biomedical materials research. Part B, Applied biomaterials</title><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><description>This article describes the use of a novel lactone‐layered double hydroxide polymer network (PN), derived from a poly(lactide‐co‐caprolactone) copolymer, as a controlled ion‐release agent for artificial bone tissue regeneration. The osteogenic cell culture Saos‐2 is used as a test culture to investigate the PN's performance as an extracellular ion‐release agent. The compelling performance of this PN is demonstrated in both growth and osteogenic media compared with a control of cells grown on tissue culture plastic (TCP) without PN. Firstly, the PNs released concentration of magnesium ions over time ranging from 10 to 60 mM after 24 hr, depending on the PN sample. After incubation of Saos‐2 with the PN, while no difference was seen in cell number, there was significant upregulation of bone‐related gene expression at 14 days—~5fold increase in Bone Morphogenetic Protein 2, ~3fold increase in osteopontin and ~2fold increase in collagen Type I. In addition, normalized alkaline phosphatase activity was seen to significantly increase by ~2fold with PN presence. A ~4fold increase in collagen Type I protein expression (via Gomori Trichrome Stain) was observed with PN presence. In addition, a ~4fold increase in phosphate deposits (as seen with Von Kossa staining analysis) was seen with PN presence. It is found that this novel PN material has a significant potential for bone tissue regeneration.</description><subject>Alkaline phosphatase</subject><subject>Biomedical materials</subject><subject>Bone growth</subject><subject>Bone morphogenetic protein 2</subject><subject>bone repair</subject><subject>Bones</subject><subject>Cell culture</subject><subject>Cell number</subject><subject>Collagen</subject><subject>Collagen (type I)</subject><subject>Copolymers</subject><subject>Gene expression</subject><subject>Ionomers</subject><subject>layered double hydroxide</subject><subject>Magnesium</subject><subject>Materials research</subject><subject>Materials science</subject><subject>Osteopontin</subject><subject>polycaprolactone</subject><subject>polylactic acid</subject><subject>Polymers</subject><subject>Proteins</subject><subject>Regeneration</subject><subject>Release agents</subject><subject>SAOS2</subject><subject>Tissue culture</subject><subject>Tissue engineering</subject><issn>1552-4973</issn><issn>1552-4981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNp90LlOAzEQBmALgUg4KnpkiQYJJayP3bVLEnGKo4Ha8jErNtqNg50lpOMReEaeBEOAgoLKU3zza_wjtEeyIckyejwx7dAMGS8IX0N9kud0wKUg679zyXpoK8ZJwkWWs03UY5SWZS5lH93d-mdocKPt3E_h_fWt0UsI4LDznWkAPy5d8C-1A1z7qW8h4JlfOAgRVz5gk3bwvI6xAxxgpuuwgzYq3UTY_X630cPZ6f34YnB9d345PrkeWM44H3BniRFUSluYXDpDSmq1E5nlhS2FKTirBDEgy6IEJgSvpM6F41QbLRkIzbbR4Sp3FvxTB3Gu2jpaaBo9Bd9FRdOWIDL9M9GDP3TiuzBN1ynKEytlkYukjlbKBh9jgErNQt3qsFQkU589q9SzMuqr56T3vzM704L7tT_FJkBXYFE3sPwvS12Nbkar1A9Q4ImH</recordid><startdate>202010</startdate><enddate>202010</enddate><creator>Zhou, Tianhao</creator><creator>McCarthy, Edward D.</creator><creator>Soutis, Constantinos</creator><creator>Cartmell, Sarah H.</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2038-2929</orcidid></search><sort><creationdate>202010</creationdate><title>Novel lactone‐layered double hydroxide ionomer powders for bone tissue repair</title><author>Zhou, Tianhao ; McCarthy, Edward D. ; Soutis, Constantinos ; Cartmell, Sarah H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4344-4dc1b8299c6b59db172cad80c46c78b643f81be9767e3884f9a58d42aba93e8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alkaline phosphatase</topic><topic>Biomedical materials</topic><topic>Bone growth</topic><topic>Bone morphogenetic protein 2</topic><topic>bone repair</topic><topic>Bones</topic><topic>Cell culture</topic><topic>Cell number</topic><topic>Collagen</topic><topic>Collagen (type I)</topic><topic>Copolymers</topic><topic>Gene expression</topic><topic>Ionomers</topic><topic>layered double hydroxide</topic><topic>Magnesium</topic><topic>Materials research</topic><topic>Materials science</topic><topic>Osteopontin</topic><topic>polycaprolactone</topic><topic>polylactic acid</topic><topic>Polymers</topic><topic>Proteins</topic><topic>Regeneration</topic><topic>Release agents</topic><topic>SAOS2</topic><topic>Tissue culture</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Tianhao</creatorcontrib><creatorcontrib>McCarthy, Edward D.</creatorcontrib><creatorcontrib>Soutis, Constantinos</creatorcontrib><creatorcontrib>Cartmell, Sarah H.</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Tianhao</au><au>McCarthy, Edward D.</au><au>Soutis, Constantinos</au><au>Cartmell, Sarah H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel lactone‐layered double hydroxide ionomer powders for bone tissue repair</atitle><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><date>2020-10</date><risdate>2020</risdate><volume>108</volume><issue>7</issue><spage>2835</spage><epage>2846</epage><pages>2835-2846</pages><issn>1552-4973</issn><eissn>1552-4981</eissn><abstract>This article describes the use of a novel lactone‐layered double hydroxide polymer network (PN), derived from a poly(lactide‐co‐caprolactone) copolymer, as a controlled ion‐release agent for artificial bone tissue regeneration. The osteogenic cell culture Saos‐2 is used as a test culture to investigate the PN's performance as an extracellular ion‐release agent. The compelling performance of this PN is demonstrated in both growth and osteogenic media compared with a control of cells grown on tissue culture plastic (TCP) without PN. Firstly, the PNs released concentration of magnesium ions over time ranging from 10 to 60 mM after 24 hr, depending on the PN sample. After incubation of Saos‐2 with the PN, while no difference was seen in cell number, there was significant upregulation of bone‐related gene expression at 14 days—~5fold increase in Bone Morphogenetic Protein 2, ~3fold increase in osteopontin and ~2fold increase in collagen Type I. In addition, normalized alkaline phosphatase activity was seen to significantly increase by ~2fold with PN presence. A ~4fold increase in collagen Type I protein expression (via Gomori Trichrome Stain) was observed with PN presence. In addition, a ~4fold increase in phosphate deposits (as seen with Von Kossa staining analysis) was seen with PN presence. It is found that this novel PN material has a significant potential for bone tissue regeneration.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>32277599</pmid><doi>10.1002/jbm.b.34614</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-2038-2929</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1552-4973 |
| ispartof | Journal of biomedical materials research. Part B, Applied biomaterials, 2020-10, Vol.108 (7), p.2835-2846 |
| issn | 1552-4973 1552-4981 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2388819227 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Alkaline phosphatase Biomedical materials Bone growth Bone morphogenetic protein 2 bone repair Bones Cell culture Cell number Collagen Collagen (type I) Copolymers Gene expression Ionomers layered double hydroxide Magnesium Materials research Materials science Osteopontin polycaprolactone polylactic acid Polymers Proteins Regeneration Release agents SAOS2 Tissue culture Tissue engineering |
| title | Novel lactone‐layered double hydroxide ionomer powders for bone tissue repair |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T14%3A25%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20lactone%E2%80%90layered%20double%20hydroxide%20ionomer%20powders%20for%20bone%20tissue%20repair&rft.jtitle=Journal%20of%20biomedical%20materials%20research.%20Part%20B,%20Applied%20biomaterials&rft.au=Zhou,%20Tianhao&rft.date=2020-10&rft.volume=108&rft.issue=7&rft.spage=2835&rft.epage=2846&rft.pages=2835-2846&rft.issn=1552-4973&rft.eissn=1552-4981&rft_id=info:doi/10.1002/jbm.b.34614&rft_dat=%3Cproquest_cross%3E2388819227%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2438879658&rft_id=info:pmid/32277599&rfr_iscdi=true |