Prevalence and molecular characterization of Cryptosporidium spp. in yaks (Bos grunniens) in Naqu, China
The intestinal protozoan specie, Cryptosporidium causes serious diarrheal syndrome in humans and animals worldwide. However, limited knowledge is known about the infection caused by this specie in yaks in Naqu. About 950 serum and 150 fecal samples were collected and assayed by using commercial ELIS...
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| Veröffentlicht in: | Microbial pathogenesis 2020-07, Vol.144, p.104190-104190, Article 104190 |
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| container_title | Microbial pathogenesis |
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| creator | Li, Kun Li, Zhixing Zeng, Zhibo Li, Aoyun Mehmood, Khalid Shahzad, Muhammad Gao, Kui Li, Jiakui |
| description | The intestinal protozoan specie, Cryptosporidium causes serious diarrheal syndrome in humans and animals worldwide. However, limited knowledge is known about the infection caused by this specie in yaks in Naqu. About 950 serum and 150 fecal samples were collected and assayed by using commercial ELISA kits and nPCR detection methods to find the prevalence and molecular characterization of Cryptosporidium spp. in yaks. Results found that 103 out of 950 (10.8%) serums were uncovered against C. parvum antibodies. In different regions, the prevalence of C. parvum in yaks were in a range from 9.1% to 16.7%, with obvious difference among the three areas (P |
| doi_str_mv | 10.1016/j.micpath.2020.104190 |
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•The prevalence of C. parvum in serum samples from yaks was 10.8%.•2 (1.3%) positive were detected via nPCR from fecal samples from yaks.•The two positive samples were sequenced and identified to be C. bovis.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2020.104190</identifier><identifier>PMID: 32272216</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - microbiology ; China - epidemiology ; Cryptosporidiosis - epidemiology ; Cryptosporidium - classification ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; Cryptosporidium specie ; Feces - microbiology ; Female ; Genetic characterization ; Male ; Phylogeny ; Prevalence ; Yaks ; Zoonoses - epidemiology ; Zoonoses - microbiology</subject><ispartof>Microbial pathogenesis, 2020-07, Vol.144, p.104190-104190, Article 104190</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-be16cc69353affdf1dfaa93b3cac1461e9a8d3d0cb8033dc538cd56753b960ac3</citedby><cites>FETCH-LOGICAL-c365t-be16cc69353affdf1dfaa93b3cac1461e9a8d3d0cb8033dc538cd56753b960ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2020.104190$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32272216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Kun</creatorcontrib><creatorcontrib>Li, Zhixing</creatorcontrib><creatorcontrib>Zeng, Zhibo</creatorcontrib><creatorcontrib>Li, Aoyun</creatorcontrib><creatorcontrib>Mehmood, Khalid</creatorcontrib><creatorcontrib>Shahzad, Muhammad</creatorcontrib><creatorcontrib>Gao, Kui</creatorcontrib><creatorcontrib>Li, Jiakui</creatorcontrib><title>Prevalence and molecular characterization of Cryptosporidium spp. in yaks (Bos grunniens) in Naqu, China</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>The intestinal protozoan specie, Cryptosporidium causes serious diarrheal syndrome in humans and animals worldwide. However, limited knowledge is known about the infection caused by this specie in yaks in Naqu. About 950 serum and 150 fecal samples were collected and assayed by using commercial ELISA kits and nPCR detection methods to find the prevalence and molecular characterization of Cryptosporidium spp. in yaks. Results found that 103 out of 950 (10.8%) serums were uncovered against C. parvum antibodies. In different regions, the prevalence of C. parvum in yaks were in a range from 9.1% to 16.7%, with obvious difference among the three areas (P < 0.001). In male and female yaks, the prevalence of C. parvum was found to be 7.2% and 13.3% respectively (P < 0.001); and a significant difference (P < 0.001) with a range of 9.8%–18.2% was observed among different age groups. Out of 150 fecal samples, only 2 (1.3%) positive samples were detected via nPCR. The positive samples were sequenced and identified to be C. bovis. The two isolates were clustered to cattle and yak clade separately. Our results highlight the prevalence and epidemiological status of Cryptosporidium spp. in yaks which may contribute towards the prevention and control of this zoonotic disease in Naqu, China.
•The prevalence of C. parvum in serum samples from yaks was 10.8%.•2 (1.3%) positive were detected via nPCR from fecal samples from yaks.•The two positive samples were sequenced and identified to be C. bovis.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - microbiology</subject><subject>China - epidemiology</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidium - classification</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium specie</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Genetic characterization</subject><subject>Male</subject><subject>Phylogeny</subject><subject>Prevalence</subject><subject>Yaks</subject><subject>Zoonoses - epidemiology</subject><subject>Zoonoses - microbiology</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVISbZpf0KKjgnUG33YWvkU2iX9gJD20J7FeCRntbElR7ID21_fNbvNtaeBl-edYR5CLjlbcsbVzXbZexxg3CwFE3NW8pqdkAVntSq4YPqULJjWoigZZ-fkbc5bxlhdyvqMnEshVkJwtSCbn8m9QOcCOgrB0j52DqcOEsUNJMDRJf8HRh8DjS1dp90wxjzE5K2fepqHYUl9oDt4yvTqc8z0MU0heBfy9Zw_wPP0ka43PsA78qaFLrv3x3lBfn-5-7X-Vtz_-Pp9_em-QKmqsWgcV4iqlpWEtrUtty1ALRuJgLxU3NWgrbQMG82ktFhJjbZSq0o2tWKA8oJcHfYOKT5PLo-m9xld10FwccpGSK01X5VK7dHqgGKKOSfXmiH5HtLOcGZmyWZrjpLNLNkcJO97H44npqZ39rX1z-oeuD0Abv_oi3fJZPSzYuuTw9HY6P9z4i_IJpGE</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Li, Kun</creator><creator>Li, Zhixing</creator><creator>Zeng, Zhibo</creator><creator>Li, Aoyun</creator><creator>Mehmood, Khalid</creator><creator>Shahzad, Muhammad</creator><creator>Gao, Kui</creator><creator>Li, Jiakui</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202007</creationdate><title>Prevalence and molecular characterization of Cryptosporidium spp. in yaks (Bos grunniens) in Naqu, China</title><author>Li, Kun ; Li, Zhixing ; Zeng, Zhibo ; Li, Aoyun ; Mehmood, Khalid ; Shahzad, Muhammad ; Gao, Kui ; Li, Jiakui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-be16cc69353affdf1dfaa93b3cac1461e9a8d3d0cb8033dc538cd56753b960ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - microbiology</topic><topic>China - epidemiology</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidium - classification</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium specie</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Genetic characterization</topic><topic>Male</topic><topic>Phylogeny</topic><topic>Prevalence</topic><topic>Yaks</topic><topic>Zoonoses - epidemiology</topic><topic>Zoonoses - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Kun</creatorcontrib><creatorcontrib>Li, Zhixing</creatorcontrib><creatorcontrib>Zeng, Zhibo</creatorcontrib><creatorcontrib>Li, Aoyun</creatorcontrib><creatorcontrib>Mehmood, Khalid</creatorcontrib><creatorcontrib>Shahzad, Muhammad</creatorcontrib><creatorcontrib>Gao, Kui</creatorcontrib><creatorcontrib>Li, Jiakui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Kun</au><au>Li, Zhixing</au><au>Zeng, Zhibo</au><au>Li, Aoyun</au><au>Mehmood, Khalid</au><au>Shahzad, Muhammad</au><au>Gao, Kui</au><au>Li, Jiakui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and molecular characterization of Cryptosporidium spp. in yaks (Bos grunniens) in Naqu, China</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2020-07</date><risdate>2020</risdate><volume>144</volume><spage>104190</spage><epage>104190</epage><pages>104190-104190</pages><artnum>104190</artnum><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>The intestinal protozoan specie, Cryptosporidium causes serious diarrheal syndrome in humans and animals worldwide. However, limited knowledge is known about the infection caused by this specie in yaks in Naqu. About 950 serum and 150 fecal samples were collected and assayed by using commercial ELISA kits and nPCR detection methods to find the prevalence and molecular characterization of Cryptosporidium spp. in yaks. Results found that 103 out of 950 (10.8%) serums were uncovered against C. parvum antibodies. In different regions, the prevalence of C. parvum in yaks were in a range from 9.1% to 16.7%, with obvious difference among the three areas (P < 0.001). In male and female yaks, the prevalence of C. parvum was found to be 7.2% and 13.3% respectively (P < 0.001); and a significant difference (P < 0.001) with a range of 9.8%–18.2% was observed among different age groups. Out of 150 fecal samples, only 2 (1.3%) positive samples were detected via nPCR. The positive samples were sequenced and identified to be C. bovis. The two isolates were clustered to cattle and yak clade separately. Our results highlight the prevalence and epidemiological status of Cryptosporidium spp. in yaks which may contribute towards the prevention and control of this zoonotic disease in Naqu, China.
•The prevalence of C. parvum in serum samples from yaks was 10.8%.•2 (1.3%) positive were detected via nPCR from fecal samples from yaks.•The two positive samples were sequenced and identified to be C. bovis.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32272216</pmid><doi>10.1016/j.micpath.2020.104190</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals Cattle Cattle Diseases - epidemiology Cattle Diseases - microbiology China - epidemiology Cryptosporidiosis - epidemiology Cryptosporidium - classification Cryptosporidium - genetics Cryptosporidium - isolation & purification Cryptosporidium specie Feces - microbiology Female Genetic characterization Male Phylogeny Prevalence Yaks Zoonoses - epidemiology Zoonoses - microbiology |
| title | Prevalence and molecular characterization of Cryptosporidium spp. in yaks (Bos grunniens) in Naqu, China |
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