Effects of ciprofloxacin on metabolic activity and algal biomass of urban stream biofilms
Pharmaceuticals and personal care products (PPCPs), such as the commonly prescribed antibiotic ciprofloxacin, are present and persistent in freshwaters, yet their effects on aquatic ecosystem functions at environmentally-relevant concentrations are rarely explored. Stream biofilms provide multiple f...
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| Veröffentlicht in: | The Science of the total environment 2020-03, Vol.706, p.135728-135728, Article 135728 |
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| creator | Gallagher, Morgan T. Reisinger, Alexander J. |
| description | Pharmaceuticals and personal care products (PPCPs), such as the commonly prescribed antibiotic ciprofloxacin, are present and persistent in freshwaters, yet their effects on aquatic ecosystem functions at environmentally-relevant concentrations are rarely explored. Stream biofilms provide multiple functions in stream ecosystems, but their functional response to PPCP contaminants such as ciprofloxacin is unclear. To establish the effect of ciprofloxacin on aquatic biofilms, we colonized biofilms in situ on tiles (n = 80) at four sites along an urban stream in Gainesville, Florida, including two sites above and two sites below a wastewater treatment plant (WWTP). We then incubated the tiles and associated biofilms in the laboratory for 6 d exposing biofilms to either 0, 0.01, 0.1, or 1.0 μg/L (target concentrations) of ciprofloxacin. At the end of the 6 d laboratory exposure, we quantified gross primary production (GPP), respiration (R), and biomass (as chlorophyll a) of biofilms, and calculated response ratios for each response. All response metrics were significantly differed across sites (p |
| doi_str_mv | 10.1016/j.scitotenv.2019.135728 |
| format | Article |
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[Display omitted]
•Effects of pharmaceuticals on ecosystem functioning are rarely studied.•Ciprofloxacin exposure inhibits gross primary production of urban stream biofilms.•Biofilm respiration was not affected by ciprofloxacin exposure.•Ciprofloxacin concentration did not change the effects of exposure.•Biofilms near a WWTP outfall responded differently than other sites.</description><identifier>ISSN: 0048-9697</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2019.135728</identifier><identifier>PMID: 31940730</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>algae ; Antibiotics ; aquatic ecosystems ; biochemical pathways ; Biofilm ; Biofilms - drug effects ; Biomass ; chlorophyll ; Chlorophyll A ; chronic exposure ; Ciprofloxacin ; Ciprofloxacin - toxicity ; drugs ; ecological function ; Ecosystem ; Ecosystem function ; Florida ; gross primary productivity ; Metabolism ; Microalgae - drug effects ; personal care products ; Pharmaceuticals ; streams ; sublethal effects ; tiles ; trophic levels ; wastewater treatment ; Water Pollutants, Chemical - toxicity</subject><ispartof>The Science of the total environment, 2020-03, Vol.706, p.135728-135728, Article 135728</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-b352df032a866f2ca812945332b5288d36af58f8cf4dddc94848f494d9fe4af93</citedby><cites>FETCH-LOGICAL-c453t-b352df032a866f2ca812945332b5288d36af58f8cf4dddc94848f494d9fe4af93</cites><orcidid>0000-0003-4096-2637</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0048969719357237$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31940730$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gallagher, Morgan T.</creatorcontrib><creatorcontrib>Reisinger, Alexander J.</creatorcontrib><title>Effects of ciprofloxacin on metabolic activity and algal biomass of urban stream biofilms</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>Pharmaceuticals and personal care products (PPCPs), such as the commonly prescribed antibiotic ciprofloxacin, are present and persistent in freshwaters, yet their effects on aquatic ecosystem functions at environmentally-relevant concentrations are rarely explored. Stream biofilms provide multiple functions in stream ecosystems, but their functional response to PPCP contaminants such as ciprofloxacin is unclear. To establish the effect of ciprofloxacin on aquatic biofilms, we colonized biofilms in situ on tiles (n = 80) at four sites along an urban stream in Gainesville, Florida, including two sites above and two sites below a wastewater treatment plant (WWTP). We then incubated the tiles and associated biofilms in the laboratory for 6 d exposing biofilms to either 0, 0.01, 0.1, or 1.0 μg/L (target concentrations) of ciprofloxacin. At the end of the 6 d laboratory exposure, we quantified gross primary production (GPP), respiration (R), and biomass (as chlorophyll a) of biofilms, and calculated response ratios for each response. All response metrics were significantly differed across sites (p < 0.01). Ciprofloxacin significantly decreased GPP (p < 0.05) regardless of treatment concentration, most notably at the site immediately below the WWTP, where there was no measurable GPP on any ciprofloxacin-treated biofilms. In contrast, respiration (R) was not significantly affected by ciprofloxacin, despite an apparent increase in R at the WWTP site. However, the WWTP site R was significantly different from the most upstream and downstream sites (p < 0.001) but was not significantly different from a nearby site upstream of the WWTP (p > 0.05). These results indicate that chronic exposure to ciprofloxacin through WWTP effluent can alter ecosystem functions performed by biofilms, which can have consequences for higher trophic levels and stream processes. By quantifying biofilm metabolic responses to ciprofloxacin exposure, this study supports the concept that pharmaceuticals and personal care products can induce sub-lethal effects on ecological processes at environmentally-relevant concentrations.
[Display omitted]
•Effects of pharmaceuticals on ecosystem functioning are rarely studied.•Ciprofloxacin exposure inhibits gross primary production of urban stream biofilms.•Biofilm respiration was not affected by ciprofloxacin exposure.•Ciprofloxacin concentration did not change the effects of exposure.•Biofilms near a WWTP outfall responded differently than other sites.</description><subject>algae</subject><subject>Antibiotics</subject><subject>aquatic ecosystems</subject><subject>biochemical pathways</subject><subject>Biofilm</subject><subject>Biofilms - drug effects</subject><subject>Biomass</subject><subject>chlorophyll</subject><subject>Chlorophyll A</subject><subject>chronic exposure</subject><subject>Ciprofloxacin</subject><subject>Ciprofloxacin - toxicity</subject><subject>drugs</subject><subject>ecological function</subject><subject>Ecosystem</subject><subject>Ecosystem function</subject><subject>Florida</subject><subject>gross primary productivity</subject><subject>Metabolism</subject><subject>Microalgae - drug effects</subject><subject>personal care products</subject><subject>Pharmaceuticals</subject><subject>streams</subject><subject>sublethal effects</subject><subject>tiles</subject><subject>trophic levels</subject><subject>wastewater treatment</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0048-9697</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1P3DAQhi0Egu3CXyg-csnWX4nHR4SgrYTUCxw4WY4_Kq-SGGzvCv59ky7lSudiafy-74zmQeiSkg0ltPu23RQba6p-2m8YoWpDeSsZHKEVBakaSlh3jFaECGhUp-QZ-lLKlswlgZ6iM06VIJKTFXq6DcHbWnAK2MbnnMKQXo2NE04THn01fRqixcbWuI_1DZvJYTP8NgPuYxpN-Wvc5d5MuNTszbj0QxzGco5OghmKv3h_1-jx7vbh5kdz_-v7z5vr-8aKltem5y1zgXBmoOsCswYoU_MPZ33LABzvTGghgA3COWeVAAFBKOFU8MIExdfo6pA7L_-y86XqMRbrh8FMPu2KZhxAAlCA_5ByJRWT7ZIqD1KbUynZB_2c42jym6ZELwj0Vn8g0AsCfUAwO7--D9n1o3cfvn83nwXXB4Gfr7KPPi9BfrLexTyj0C7FT4f8AYv8nLQ</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Gallagher, Morgan T.</creator><creator>Reisinger, Alexander J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-4096-2637</orcidid></search><sort><creationdate>20200301</creationdate><title>Effects of ciprofloxacin on metabolic activity and algal biomass of urban stream biofilms</title><author>Gallagher, Morgan T. ; Reisinger, Alexander J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-b352df032a866f2ca812945332b5288d36af58f8cf4dddc94848f494d9fe4af93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>algae</topic><topic>Antibiotics</topic><topic>aquatic ecosystems</topic><topic>biochemical pathways</topic><topic>Biofilm</topic><topic>Biofilms - drug effects</topic><topic>Biomass</topic><topic>chlorophyll</topic><topic>Chlorophyll A</topic><topic>chronic exposure</topic><topic>Ciprofloxacin</topic><topic>Ciprofloxacin - toxicity</topic><topic>drugs</topic><topic>ecological function</topic><topic>Ecosystem</topic><topic>Ecosystem function</topic><topic>Florida</topic><topic>gross primary productivity</topic><topic>Metabolism</topic><topic>Microalgae - drug effects</topic><topic>personal care products</topic><topic>Pharmaceuticals</topic><topic>streams</topic><topic>sublethal effects</topic><topic>tiles</topic><topic>trophic levels</topic><topic>wastewater treatment</topic><topic>Water Pollutants, Chemical - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gallagher, Morgan T.</creatorcontrib><creatorcontrib>Reisinger, Alexander J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gallagher, Morgan T.</au><au>Reisinger, Alexander J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of ciprofloxacin on metabolic activity and algal biomass of urban stream biofilms</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>706</volume><spage>135728</spage><epage>135728</epage><pages>135728-135728</pages><artnum>135728</artnum><issn>0048-9697</issn><eissn>1879-1026</eissn><abstract>Pharmaceuticals and personal care products (PPCPs), such as the commonly prescribed antibiotic ciprofloxacin, are present and persistent in freshwaters, yet their effects on aquatic ecosystem functions at environmentally-relevant concentrations are rarely explored. Stream biofilms provide multiple functions in stream ecosystems, but their functional response to PPCP contaminants such as ciprofloxacin is unclear. To establish the effect of ciprofloxacin on aquatic biofilms, we colonized biofilms in situ on tiles (n = 80) at four sites along an urban stream in Gainesville, Florida, including two sites above and two sites below a wastewater treatment plant (WWTP). We then incubated the tiles and associated biofilms in the laboratory for 6 d exposing biofilms to either 0, 0.01, 0.1, or 1.0 μg/L (target concentrations) of ciprofloxacin. At the end of the 6 d laboratory exposure, we quantified gross primary production (GPP), respiration (R), and biomass (as chlorophyll a) of biofilms, and calculated response ratios for each response. All response metrics were significantly differed across sites (p < 0.01). Ciprofloxacin significantly decreased GPP (p < 0.05) regardless of treatment concentration, most notably at the site immediately below the WWTP, where there was no measurable GPP on any ciprofloxacin-treated biofilms. In contrast, respiration (R) was not significantly affected by ciprofloxacin, despite an apparent increase in R at the WWTP site. However, the WWTP site R was significantly different from the most upstream and downstream sites (p < 0.001) but was not significantly different from a nearby site upstream of the WWTP (p > 0.05). These results indicate that chronic exposure to ciprofloxacin through WWTP effluent can alter ecosystem functions performed by biofilms, which can have consequences for higher trophic levels and stream processes. By quantifying biofilm metabolic responses to ciprofloxacin exposure, this study supports the concept that pharmaceuticals and personal care products can induce sub-lethal effects on ecological processes at environmentally-relevant concentrations.
[Display omitted]
•Effects of pharmaceuticals on ecosystem functioning are rarely studied.•Ciprofloxacin exposure inhibits gross primary production of urban stream biofilms.•Biofilm respiration was not affected by ciprofloxacin exposure.•Ciprofloxacin concentration did not change the effects of exposure.•Biofilms near a WWTP outfall responded differently than other sites.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31940730</pmid><doi>10.1016/j.scitotenv.2019.135728</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-4096-2637</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | algae Antibiotics aquatic ecosystems biochemical pathways Biofilm Biofilms - drug effects Biomass chlorophyll Chlorophyll A chronic exposure Ciprofloxacin Ciprofloxacin - toxicity drugs ecological function Ecosystem Ecosystem function Florida gross primary productivity Metabolism Microalgae - drug effects personal care products Pharmaceuticals streams sublethal effects tiles trophic levels wastewater treatment Water Pollutants, Chemical - toxicity |
| title | Effects of ciprofloxacin on metabolic activity and algal biomass of urban stream biofilms |
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