New visible light excitable donor-acceptor 7-hydroxy-coumarins as blue fluorescent probes for selective staining of vacuoles in yeasts and L. donovani

In order to address the existing limitations of the commercially available fluorescent probe CMAC (7-amino-4-chloromethylcoumarin), a new series of highly fluorescent donor-acceptor 7-hydroxy-coumarin derivatives was prepared and these derivatives were used as vacuole specific fluorescent probes for...

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Veröffentlicht in:	Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2017-04, Vol.5 (14), p.2580-2587
Hauptverfasser:	Raghuvanshi, Ashutosh, Kumar Jha, Ajay, Kathuria, Manoj, Priya Awasthi, Bhanu, Purohit, Deepak, Mitra, Kalyan, Goel, Atul
Format:	Artikel
Sprache:	eng
Schlagworte:	Biology Derivatives Excitation Fluorescence Materials selection Protozoa Toxicity Yeast
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container_title	Journal of materials chemistry. B, Materials for biology and medicine
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creator	Raghuvanshi, Ashutosh Kumar Jha, Ajay Kathuria, Manoj Priya Awasthi, Bhanu Purohit, Deepak Mitra, Kalyan Goel, Atul
description	In order to address the existing limitations of the commercially available fluorescent probe CMAC (7-amino-4-chloromethylcoumarin), a new series of highly fluorescent donor-acceptor 7-hydroxy-coumarin derivatives was prepared and these derivatives were used as vacuole specific fluorescent probes for live cell imaging of unicellular parasitic protozoa L. donovani promastigotes and yeast cells S. pombe and S. cerevisiae. The synthesized 7-hydroxy-coumarins exhibited interesting photophysical properties and have advantages such as excitation in the visible region, good water solubility, photo-stability, good quantum yield and low cytotoxicity.
doi_str_mv	10.1039/c6tb03257e
format	Article
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source	Royal Society Of Chemistry Journals
subjects	Biology Derivatives Excitation Fluorescence Materials selection Protozoa Toxicity Yeast
title	New visible light excitable donor-acceptor 7-hydroxy-coumarins as blue fluorescent probes for selective staining of vacuoles in yeasts and L. donovani
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