Ultrapure laser-synthesized Si nanoparticles with variable oxidation states for biomedical applications

We employ a method of femtosecond laser fragmentation of preliminarily prepared water-dispersed microcolloids to fabricate aqueous solutions of ultrapure bare Si-based nanoparticles (Si-NPs) and assess their potential for biomedical applications. The nanoparticles appear spherical in shape, with low...

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Veröffentlicht in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2016-12, Vol.4 (48), p.7852-7858
Hauptverfasser: Al-Kattan, Ahmed, Ryabchikov, Yury V, Baati, Tarek, Chirvony, Vladimir, Sánchez-Royo, Juan F, Sentis, Marc, Braguer, Diane, Timoshenko, Victor Yu, Estève, Marie-Anne, Kabashin, Andrei V
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Sprache:eng
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Zusammenfassung:We employ a method of femtosecond laser fragmentation of preliminarily prepared water-dispersed microcolloids to fabricate aqueous solutions of ultrapure bare Si-based nanoparticles (Si-NPs) and assess their potential for biomedical applications. The nanoparticles appear spherical in shape, with low size dispersion and a controllable mean size, from a few nm to several tens of nm, while a negative surface charge (−35 mV ± 0.10 according to z -potential data) provides good electrostatic stabilization of colloidal Si-NP solutions. Structural analysis shows that the Si-NPs are composed of Si nanocrystals with inclusions of silicon oxide species, covered by a SiO x (1 < x < 2) shell, while the total oxide content depends on whether the fragmentation is performed in normal oxygen-saturated water (oxygen-rich conditions) or in water deoxygenated by pumping with noble gases (Ag or He) before and during the experiment (oxygen-free conditions). Our dissolution tests show the excellent water-solubility of all the NPs, while more oxidized NPs demonstrate much faster dissolution kinetics, which is explained by oxidation-induced defects in the core of the Si-NPs. Finally, by examining the interaction of the NPs with human cells after 72 h of incubation at different concentrations, we report the absence of any adverse effects of the NPs up to high concentrations (50 μg mL −1 ) and a good internalization of NPs via a classical endocytosis mechanism. Possessing far superior purity compared to their chemically synthesized counterparts and enabling a variety of imaging and therapeutic functionalities, the laser-synthesized Si-NPs are promising for safe and efficient applications in nanomedicine. We use femtosecond laser fragmentation to fabricate ultrapure bare Si-based nanoparticles (Si-NPs) for biomedical applications.
ISSN:2050-750X
2050-7518
DOI:10.1039/c6tb02623k