Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal epithelial regeneration
As a strategy to improve the outcome of cultivated corneal epithelial transplantation, the role of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) is investigated in promoting corneal epithelial growth and functions. Human telomerase-immortalized corneal epithelial cells were characte...
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| Veröffentlicht in: | Regenerative medicine 2020-03, Vol.15 (3), p.1381-1397 |
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| container_title | Regenerative medicine |
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| creator | Azmi, Siti Maisura Salih, Mohamed Abdelrazeg, Samar Roslan, Fatin Fazrina Mohamed, Rafeezul Jie, Tan Jun Shaharuddin, Bakiah |
| description | As a strategy to improve the outcome of
cultivated corneal epithelial transplantation, the role of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) is investigated in promoting corneal epithelial growth and functions.
Human telomerase-immortalized corneal epithelial cells were characterized and its functions evaluated by scratch migration assay, cellular senescence, HLA expression and spheres formation with hUC-MSC.
Expression of corneal epithelial markers was influenced by the duration and method of co-culture. Indirect co-culture improved cellular migration and delayed senescence when treated after 3 and 5 days. hUC-MSC downregulated expression of HLA Class I and II in IFN-γ-stimulated human telomerase-immortalized corneal epithelial cells.
hUC-MSC promote corneal epithelial growth and functions after treatment with hUC-MSC. |
| doi_str_mv | 10.2217/rme-2019-0103 |
| format | Article |
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cultivated corneal epithelial transplantation, the role of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) is investigated in promoting corneal epithelial growth and functions.
Human telomerase-immortalized corneal epithelial cells were characterized and its functions evaluated by scratch migration assay, cellular senescence, HLA expression and spheres formation with hUC-MSC.
Expression of corneal epithelial markers was influenced by the duration and method of co-culture. Indirect co-culture improved cellular migration and delayed senescence when treated after 3 and 5 days. hUC-MSC downregulated expression of HLA Class I and II in IFN-γ-stimulated human telomerase-immortalized corneal epithelial cells.
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cultivated corneal epithelial transplantation, the role of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) is investigated in promoting corneal epithelial growth and functions.
Human telomerase-immortalized corneal epithelial cells were characterized and its functions evaluated by scratch migration assay, cellular senescence, HLA expression and spheres formation with hUC-MSC.
Expression of corneal epithelial markers was influenced by the duration and method of co-culture. Indirect co-culture improved cellular migration and delayed senescence when treated after 3 and 5 days. hUC-MSC downregulated expression of HLA Class I and II in IFN-γ-stimulated human telomerase-immortalized corneal epithelial cells.
hUC-MSC promote corneal epithelial growth and functions after treatment with hUC-MSC.</description><subject>corneal characterization</subject><subject>corneal epithelial cells</subject><subject>ocular surface regeneration</subject><subject>umbilical cord derived-mesenchymal stem cells</subject><issn>1746-0751</issn><issn>1746-076X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kD1PwzAQhi0EoqUwsqKMLAF_xHXChiqgSJVYQGKz_HFpjeKk2MnQf4-jFDYmn0_Pnd57ELom-I5SIu6Dh5xiUuWYYHaC5kQUyxyL5efpX83JDF3E-IUxZyWn52jGKOWsEsUcwXrwqs0Gr13jjGoy0wWbe4jQmt3Bp0bswWcGmiY-ZCrbh8676Npt6gfVw_aQ1V0Yp1pIMOxdv4PGpTLAFlpIjOvaS3RWqybC1fFdoI_np_fVOt-8vbyuHje5YQXrcwOKcm2YFjodU4IQFEhRayLYckl0ybSqLMWVtbRWpQZTMGsF41Zhyykr2QLdTntTzO8BYi9T2DG7aqEbokyMoLwqCE1oPqEmdDEGqOU-OK_CQRIsR7MymZWjWTmaTfzNcfWgPdg_-ldlAqoJqId-CBCNSwpBTr804Yxr4Z_lP0i_igI</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Azmi, Siti Maisura</creator><creator>Salih, Mohamed</creator><creator>Abdelrazeg, Samar</creator><creator>Roslan, Fatin Fazrina</creator><creator>Mohamed, Rafeezul</creator><creator>Jie, Tan Jun</creator><creator>Shaharuddin, Bakiah</creator><general>Future Medicine Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6191-8964</orcidid></search><sort><creationdate>20200301</creationdate><title>Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal epithelial regeneration</title><author>Azmi, Siti Maisura ; Salih, Mohamed ; Abdelrazeg, Samar ; Roslan, Fatin Fazrina ; Mohamed, Rafeezul ; Jie, Tan Jun ; Shaharuddin, Bakiah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-cea25bc3b7b1038e772e14fb173661b83ba9d209dd2fa8bec43dd735da0d52383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>corneal characterization</topic><topic>corneal epithelial cells</topic><topic>ocular surface regeneration</topic><topic>umbilical cord derived-mesenchymal stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azmi, Siti Maisura</creatorcontrib><creatorcontrib>Salih, Mohamed</creatorcontrib><creatorcontrib>Abdelrazeg, Samar</creatorcontrib><creatorcontrib>Roslan, Fatin Fazrina</creatorcontrib><creatorcontrib>Mohamed, Rafeezul</creatorcontrib><creatorcontrib>Jie, Tan Jun</creatorcontrib><creatorcontrib>Shaharuddin, Bakiah</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Regenerative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azmi, Siti Maisura</au><au>Salih, Mohamed</au><au>Abdelrazeg, Samar</au><au>Roslan, Fatin Fazrina</au><au>Mohamed, Rafeezul</au><au>Jie, Tan Jun</au><au>Shaharuddin, Bakiah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal epithelial regeneration</atitle><jtitle>Regenerative medicine</jtitle><addtitle>Regen Med</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>15</volume><issue>3</issue><spage>1381</spage><epage>1397</epage><pages>1381-1397</pages><issn>1746-0751</issn><eissn>1746-076X</eissn><abstract>As a strategy to improve the outcome of
cultivated corneal epithelial transplantation, the role of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) is investigated in promoting corneal epithelial growth and functions.
Human telomerase-immortalized corneal epithelial cells were characterized and its functions evaluated by scratch migration assay, cellular senescence, HLA expression and spheres formation with hUC-MSC.
Expression of corneal epithelial markers was influenced by the duration and method of co-culture. Indirect co-culture improved cellular migration and delayed senescence when treated after 3 and 5 days. hUC-MSC downregulated expression of HLA Class I and II in IFN-γ-stimulated human telomerase-immortalized corneal epithelial cells.
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| ispartof | Regenerative medicine, 2020-03, Vol.15 (3), p.1381-1397 |
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| language | eng |
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| source | PubMed Central |
| subjects | corneal characterization corneal epithelial cells ocular surface regeneration umbilical cord derived-mesenchymal stem cells |
| title | Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal epithelial regeneration |
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