Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9–Cas12a platform

Systematic mapping of genetic interactions (GIs) and interrogation of the functions of sizable genomic segments in mammalian cells represent important goals of biomedical research. To advance these goals, we present a CRISPR (clustered regularly interspaced short palindromic repeats)-based screening...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature biotechnology 2020-05, Vol.38 (5), p.638-648
Hauptverfasser:	Gonatopoulos-Pournatzis, Thomas, Aregger, Michael, Brown, Kevin R., Farhangmehr, Shaghayegh, Braunschweig, Ulrich, Ward, Henry N., Ha, Kevin C. H., Weiss, Alexander, Billmann, Maximilian, Durbic, Tanja, Myers, Chad L., Blencowe, Benjamin J., Moffat, Jason
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1513/1967/3196 631/337 631/553 631/61 631/80 Ablation Agriculture Alternative Splicing Animals Bacterial Proteins - metabolism Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cell Line Combinatorial analysis CRISPR CRISPR-Associated Protein 9 - metabolism CRISPR-Associated Proteins - metabolism CRISPR-Cas Systems Editing Endodeoxyribonucleases - metabolism Exons Functional analysis Gene Editing - methods Gene mapping Gene Regulatory Networks Genetic Fitness Genetic screening Genomic analysis Genomics Humans Hybrid systems Interrogation Learning algorithms Life Sciences Machine Learning Male Mammalian cells Mammals Mapping Mice Nuclease Phenotypes Redundancy Screening Signal Transduction Splicing TOR protein TOR Serine-Threonine Kinases - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!