MiR‐342 attenuates lipopolysaccharide‐induced acute lung injury via inhibiting MAPK1 expression
Micro RNA (miRNA) and mitogen‐activated protein kinase (MAPK) are reported as the crucial regulators of inflammatory responses in acute lung injury (ALI). This study will explore the role of the miR‐342/MAPK1 axis in regulation of lipopolysaccharide (LPS)‐induced ALI. We found that miR‐342 was down‐...
Gespeichert in:
| Veröffentlicht in: | Clinical and experimental pharmacology & physiology 2020-08, Vol.47 (8), p.1448-1454 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1454 |
|---|---|
| container_issue | 8 |
| container_start_page | 1448 |
| container_title | Clinical and experimental pharmacology & physiology |
| container_volume | 47 |
| creator | Zhu, Siliang Song, Wenke Sun, Yanqi Zhou, Yongqin Kong, Fanpo |
| description | Micro RNA (miRNA) and mitogen‐activated protein kinase (MAPK) are reported as the crucial regulators of inflammatory responses in acute lung injury (ALI). This study will explore the role of the miR‐342/MAPK1 axis in regulation of lipopolysaccharide (LPS)‐induced ALI. We found that miR‐342 was down‐regulated in LPS‐induced A549 cells compared with the control group with DMSO, accompanied by elevated inflammatory cytokines and apoptosis. Over‐expression of miR‐342 reduced LPS‐induced inflammatory responses and apoptosis in LPS‐stimulated A549 cells, and had a protective role in LPS‐treated mice with ALI by decreasing levels of inflammatory cytokines, improving survival of mice with ALI, and ameliorating the lung permeability. Dual‐luciferase reporter gene assay demonstrated that miR‐342 regulated the expression of MAPK1 by directly targeting its 3′ untranslated region (3′‐UTR). Mechanistically, MAPK1 silencing abrogated LPS‐induced inflammatory injury in A549 cells, and partially enhanced the protective effect of miR‐342. Therefore, miR‐342 attenuates LPS‐induced ALI by targeting MAPK1 expression, thereby protecting against A549 cell injury induced by LPS and lung injury of mice with ALI. |
| doi_str_mv | 10.1111/1440-1681.13315 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2386434290</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2419191030</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4405-3e9f704d591ed5fd35ff259e3688554841d9b308df315e3b017e13cbdf4d48c43</originalsourceid><addsrcrecordid>eNqFkL9OwzAQhy0EglKY2VAkFpYUO7ZbZ0RV-SOoQAhmy7Ev1FWaBDsGuvEIPCNPgkuBgQV78On0-ae7D6EDggcknhPCGE7JUJABoZTwDdT77WyiHqaYp0SM8A7a9X6OMeZ4SLfRDs0yJjjjPaSn9u7j7Z2yLFFdB3VQHfiksm3TNtXSK61nylkDkbG1CRpMonToIKlC_ZjYeh7cMnm2KpYzW9jOxu709PaKJPDaOvDeNvUe2ipV5WH_--2jh7PJ_fgivb45vxyfXqc6zsxTCnk5wszwnIDhpaG8LDOeAx0KwTkTjJi8oFiYMm4KtMBkBITqwpTMMKEZ7aPjdW7rmqcAvpML6zVUlaqhCV5mVAxZ3DTHET36g86b4Oo4ncwYyeON7iJ1sqa0a7x3UMrW2YVyS0mwXPmXK9tyZVt--Y8_Dr9zQ7EA88v_CI8AXwMvtoLlf3lyPLldB38CNfCQDA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2419191030</pqid></control><display><type>article</type><title>MiR‐342 attenuates lipopolysaccharide‐induced acute lung injury via inhibiting MAPK1 expression</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><creator>Zhu, Siliang ; Song, Wenke ; Sun, Yanqi ; Zhou, Yongqin ; Kong, Fanpo</creator><creatorcontrib>Zhu, Siliang ; Song, Wenke ; Sun, Yanqi ; Zhou, Yongqin ; Kong, Fanpo</creatorcontrib><description>Micro RNA (miRNA) and mitogen‐activated protein kinase (MAPK) are reported as the crucial regulators of inflammatory responses in acute lung injury (ALI). This study will explore the role of the miR‐342/MAPK1 axis in regulation of lipopolysaccharide (LPS)‐induced ALI. We found that miR‐342 was down‐regulated in LPS‐induced A549 cells compared with the control group with DMSO, accompanied by elevated inflammatory cytokines and apoptosis. Over‐expression of miR‐342 reduced LPS‐induced inflammatory responses and apoptosis in LPS‐stimulated A549 cells, and had a protective role in LPS‐treated mice with ALI by decreasing levels of inflammatory cytokines, improving survival of mice with ALI, and ameliorating the lung permeability. Dual‐luciferase reporter gene assay demonstrated that miR‐342 regulated the expression of MAPK1 by directly targeting its 3′ untranslated region (3′‐UTR). Mechanistically, MAPK1 silencing abrogated LPS‐induced inflammatory injury in A549 cells, and partially enhanced the protective effect of miR‐342. Therefore, miR‐342 attenuates LPS‐induced ALI by targeting MAPK1 expression, thereby protecting against A549 cell injury induced by LPS and lung injury of mice with ALI.</description><identifier>ISSN: 0305-1870</identifier><identifier>ISSN: 1440-1681</identifier><identifier>EISSN: 1440-1681</identifier><identifier>DOI: 10.1111/1440-1681.13315</identifier><identifier>PMID: 32248545</identifier><language>eng</language><publisher>Australia: Wiley Subscription Services, Inc</publisher><subject>3' Untranslated regions ; A549 Cells ; Acute Lung Injury - chemically induced ; Acute Lung Injury - genetics ; Acute Lung Injury - metabolism ; Acute Lung Injury - pathology ; ALI ; Animals ; Apoptosis ; Apoptosis - drug effects ; Cell injury ; Cytokines ; Gene expression ; Humans ; Inflammation ; Injuries ; Kinases ; Lipopolysaccharides ; Lipopolysaccharides - toxicity ; Lungs ; Male ; MAP kinase ; MAPK1 ; Mice ; Mice, Inbred C57BL ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; miR‐342 ; Mitogen-Activated Protein Kinase 1 - genetics ; Mitogen-Activated Protein Kinase 1 - metabolism ; Permeability ; Protein kinase ; Regulators ; Reporter gene ; Ribonucleic acid ; RNA</subject><ispartof>Clinical and experimental pharmacology & physiology, 2020-08, Vol.47 (8), p.1448-1454</ispartof><rights>2020 John Wiley & Sons Australia, Ltd</rights><rights>2020 John Wiley & Sons Australia, Ltd.</rights><rights>Copyright © 2020 John Wiley & Sons Australia, Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4405-3e9f704d591ed5fd35ff259e3688554841d9b308df315e3b017e13cbdf4d48c43</citedby><cites>FETCH-LOGICAL-c4405-3e9f704d591ed5fd35ff259e3688554841d9b308df315e3b017e13cbdf4d48c43</cites><orcidid>0000-0001-8791-6764</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1440-1681.13315$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1440-1681.13315$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32248545$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Siliang</creatorcontrib><creatorcontrib>Song, Wenke</creatorcontrib><creatorcontrib>Sun, Yanqi</creatorcontrib><creatorcontrib>Zhou, Yongqin</creatorcontrib><creatorcontrib>Kong, Fanpo</creatorcontrib><title>MiR‐342 attenuates lipopolysaccharide‐induced acute lung injury via inhibiting MAPK1 expression</title><title>Clinical and experimental pharmacology & physiology</title><addtitle>Clin Exp Pharmacol Physiol</addtitle><description>Micro RNA (miRNA) and mitogen‐activated protein kinase (MAPK) are reported as the crucial regulators of inflammatory responses in acute lung injury (ALI). This study will explore the role of the miR‐342/MAPK1 axis in regulation of lipopolysaccharide (LPS)‐induced ALI. We found that miR‐342 was down‐regulated in LPS‐induced A549 cells compared with the control group with DMSO, accompanied by elevated inflammatory cytokines and apoptosis. Over‐expression of miR‐342 reduced LPS‐induced inflammatory responses and apoptosis in LPS‐stimulated A549 cells, and had a protective role in LPS‐treated mice with ALI by decreasing levels of inflammatory cytokines, improving survival of mice with ALI, and ameliorating the lung permeability. Dual‐luciferase reporter gene assay demonstrated that miR‐342 regulated the expression of MAPK1 by directly targeting its 3′ untranslated region (3′‐UTR). Mechanistically, MAPK1 silencing abrogated LPS‐induced inflammatory injury in A549 cells, and partially enhanced the protective effect of miR‐342. Therefore, miR‐342 attenuates LPS‐induced ALI by targeting MAPK1 expression, thereby protecting against A549 cell injury induced by LPS and lung injury of mice with ALI.</description><subject>3' Untranslated regions</subject><subject>A549 Cells</subject><subject>Acute Lung Injury - chemically induced</subject><subject>Acute Lung Injury - genetics</subject><subject>Acute Lung Injury - metabolism</subject><subject>Acute Lung Injury - pathology</subject><subject>ALI</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Cell injury</subject><subject>Cytokines</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Injuries</subject><subject>Kinases</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - toxicity</subject><subject>Lungs</subject><subject>Male</subject><subject>MAP kinase</subject><subject>MAPK1</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>miR‐342</subject><subject>Mitogen-Activated Protein Kinase 1 - genetics</subject><subject>Mitogen-Activated Protein Kinase 1 - metabolism</subject><subject>Permeability</subject><subject>Protein kinase</subject><subject>Regulators</subject><subject>Reporter gene</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><issn>0305-1870</issn><issn>1440-1681</issn><issn>1440-1681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL9OwzAQhy0EglKY2VAkFpYUO7ZbZ0RV-SOoQAhmy7Ev1FWaBDsGuvEIPCNPgkuBgQV78On0-ae7D6EDggcknhPCGE7JUJABoZTwDdT77WyiHqaYp0SM8A7a9X6OMeZ4SLfRDs0yJjjjPaSn9u7j7Z2yLFFdB3VQHfiksm3TNtXSK61nylkDkbG1CRpMonToIKlC_ZjYeh7cMnm2KpYzW9jOxu709PaKJPDaOvDeNvUe2ipV5WH_--2jh7PJ_fgivb45vxyfXqc6zsxTCnk5wszwnIDhpaG8LDOeAx0KwTkTjJi8oFiYMm4KtMBkBITqwpTMMKEZ7aPjdW7rmqcAvpML6zVUlaqhCV5mVAxZ3DTHET36g86b4Oo4ncwYyeON7iJ1sqa0a7x3UMrW2YVyS0mwXPmXK9tyZVt--Y8_Dr9zQ7EA88v_CI8AXwMvtoLlf3lyPLldB38CNfCQDA</recordid><startdate>202008</startdate><enddate>202008</enddate><creator>Zhu, Siliang</creator><creator>Song, Wenke</creator><creator>Sun, Yanqi</creator><creator>Zhou, Yongqin</creator><creator>Kong, Fanpo</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8791-6764</orcidid></search><sort><creationdate>202008</creationdate><title>MiR‐342 attenuates lipopolysaccharide‐induced acute lung injury via inhibiting MAPK1 expression</title><author>Zhu, Siliang ; Song, Wenke ; Sun, Yanqi ; Zhou, Yongqin ; Kong, Fanpo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4405-3e9f704d591ed5fd35ff259e3688554841d9b308df315e3b017e13cbdf4d48c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>3' Untranslated regions</topic><topic>A549 Cells</topic><topic>Acute Lung Injury - chemically induced</topic><topic>Acute Lung Injury - genetics</topic><topic>Acute Lung Injury - metabolism</topic><topic>Acute Lung Injury - pathology</topic><topic>ALI</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Cell injury</topic><topic>Cytokines</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Injuries</topic><topic>Kinases</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - toxicity</topic><topic>Lungs</topic><topic>Male</topic><topic>MAP kinase</topic><topic>MAPK1</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>miR‐342</topic><topic>Mitogen-Activated Protein Kinase 1 - genetics</topic><topic>Mitogen-Activated Protein Kinase 1 - metabolism</topic><topic>Permeability</topic><topic>Protein kinase</topic><topic>Regulators</topic><topic>Reporter gene</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Siliang</creatorcontrib><creatorcontrib>Song, Wenke</creatorcontrib><creatorcontrib>Sun, Yanqi</creatorcontrib><creatorcontrib>Zhou, Yongqin</creatorcontrib><creatorcontrib>Kong, Fanpo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental pharmacology & physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Siliang</au><au>Song, Wenke</au><au>Sun, Yanqi</au><au>Zhou, Yongqin</au><au>Kong, Fanpo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR‐342 attenuates lipopolysaccharide‐induced acute lung injury via inhibiting MAPK1 expression</atitle><jtitle>Clinical and experimental pharmacology & physiology</jtitle><addtitle>Clin Exp Pharmacol Physiol</addtitle><date>2020-08</date><risdate>2020</risdate><volume>47</volume><issue>8</issue><spage>1448</spage><epage>1454</epage><pages>1448-1454</pages><issn>0305-1870</issn><issn>1440-1681</issn><eissn>1440-1681</eissn><abstract>Micro RNA (miRNA) and mitogen‐activated protein kinase (MAPK) are reported as the crucial regulators of inflammatory responses in acute lung injury (ALI). This study will explore the role of the miR‐342/MAPK1 axis in regulation of lipopolysaccharide (LPS)‐induced ALI. We found that miR‐342 was down‐regulated in LPS‐induced A549 cells compared with the control group with DMSO, accompanied by elevated inflammatory cytokines and apoptosis. Over‐expression of miR‐342 reduced LPS‐induced inflammatory responses and apoptosis in LPS‐stimulated A549 cells, and had a protective role in LPS‐treated mice with ALI by decreasing levels of inflammatory cytokines, improving survival of mice with ALI, and ameliorating the lung permeability. Dual‐luciferase reporter gene assay demonstrated that miR‐342 regulated the expression of MAPK1 by directly targeting its 3′ untranslated region (3′‐UTR). Mechanistically, MAPK1 silencing abrogated LPS‐induced inflammatory injury in A549 cells, and partially enhanced the protective effect of miR‐342. Therefore, miR‐342 attenuates LPS‐induced ALI by targeting MAPK1 expression, thereby protecting against A549 cell injury induced by LPS and lung injury of mice with ALI.</abstract><cop>Australia</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32248545</pmid><doi>10.1111/1440-1681.13315</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-8791-6764</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1870 |
| ispartof | Clinical and experimental pharmacology & physiology, 2020-08, Vol.47 (8), p.1448-1454 |
| issn | 0305-1870 1440-1681 1440-1681 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2386434290 |
| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | 3' Untranslated regions A549 Cells Acute Lung Injury - chemically induced Acute Lung Injury - genetics Acute Lung Injury - metabolism Acute Lung Injury - pathology ALI Animals Apoptosis Apoptosis - drug effects Cell injury Cytokines Gene expression Humans Inflammation Injuries Kinases Lipopolysaccharides Lipopolysaccharides - toxicity Lungs Male MAP kinase MAPK1 Mice Mice, Inbred C57BL MicroRNAs - genetics MicroRNAs - metabolism miRNA miR‐342 Mitogen-Activated Protein Kinase 1 - genetics Mitogen-Activated Protein Kinase 1 - metabolism Permeability Protein kinase Regulators Reporter gene Ribonucleic acid RNA |
| title | MiR‐342 attenuates lipopolysaccharide‐induced acute lung injury via inhibiting MAPK1 expression |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T10%3A45%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MiR%E2%80%90342%20attenuates%20lipopolysaccharide%E2%80%90induced%20acute%20lung%20injury%20via%20inhibiting%20MAPK1%20expression&rft.jtitle=Clinical%20and%20experimental%20pharmacology%20&%20physiology&rft.au=Zhu,%20Siliang&rft.date=2020-08&rft.volume=47&rft.issue=8&rft.spage=1448&rft.epage=1454&rft.pages=1448-1454&rft.issn=0305-1870&rft.eissn=1440-1681&rft_id=info:doi/10.1111/1440-1681.13315&rft_dat=%3Cproquest_cross%3E2419191030%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2419191030&rft_id=info:pmid/32248545&rfr_iscdi=true |