The immune enhancement effects of recombinant NDV expressing chicken granulocyte‐macrophage colony‐stimulating factor on the different avian influenza vaccine subtypes
Avian influenza is an acute and highly contagious infectious disease that is caused by the influenza virus. Avian influenza has been widely spread all over the world, has caused property loss and has threatened human life and security. In this study, the recombinant plasmid rClone30‐chGM‐CSF was con...
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| Veröffentlicht in: | Transboundary and emerging diseases 2020-09, Vol.67 (5), p.2108-2117 |
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| creator | Guo, Xiaochen Zhang, Teng Wang, Xiangxiang Su, Han Sun, Wenying Liu, Yunye Kang, Kai Liu, Tianyan Jiang, Shan Wang, Yaoqun Wang, Dan Yin, He Tian, Limin Li, Deshan Ren, Guiping |
| description | Avian influenza is an acute and highly contagious infectious disease that is caused by the influenza virus. Avian influenza has been widely spread all over the world, has caused property loss and has threatened human life and security. In this study, the recombinant plasmid rClone30‐chGM‐CSF was constructed and rescued to the recombinant virus rClone30‐chGM‐CSF successfully. After 8 days of immunization with the recombinant virus, the titre of NDV HI (haemagglutination inhibition) antibodies in SPF chickens reached its peak. The average titre of the rClone30‐chGM‐CSF group reached 6 log2 and was significantly higher than the protection critical value of 4 log2; the titres of the rClone30 group and the blank group were 2.86 log2 and 1 log2, respectively, indicating that the recombinant virus can effectively improve the NDV antibody titre. Then, SPF chickens were co‐immunized with the recombinant virus and with three different vaccine subtypes of inactivated avian influenza. The results indicated that the SPF chickens that were immunized with the vaccine plus rClone30‐chGM‐CSF showed significantly higher avian influenza antibody levels than those in the single vaccine groups. Furthermore, the SPF chickens in the vaccine plus rClone30‐chGM‐CSF group elicited stronger CD4+ and CD8+ T‐cell proliferative responses and also had upregulated transcriptional levels of interleukin‐1β (IL‐1β), IL‐4, IL‐6 and IL‐17 compared with those in the single vaccine groups. This study has shown that the recombinant virus expressing chicken granulocyte‐macrophage colony‐stimulating factor (chGM‐CSF) can be used not only as an NDV vaccine to effectively improve the titre of NDV antibodies but also as a biological adjuvant to enhance the immune effects of the avian influenza vaccine. Therefore, this recombinant virus can also be used as a biological adjuvant for other poultry vaccines. |
| doi_str_mv | 10.1111/tbed.13559 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2386289859</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2386289859</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3939-e2323f1275a73cddfb6bd4bb22efb0a73c3f6ca006895e0a7ae209a0b82b61003</originalsourceid><addsrcrecordid>eNp9kUmO1DAYhSMEogfYcABkiQ1CqsZD4kqW9MAgtWBTsLVs53eVm8QOHhrSK47APbgVJ8GhulmwwBtbT5_f-_W_qnpC8Akp52VS0J8Q1jTdveqQtLxZEd7S-3_f6_qgOorxCmOOO948rA4YpTVvODmsfm52gOw4ZgcI3E46DSO4hMAY0Ckib1AA7UdlnSzy-_NPCL5NAWK0bov0zurP4NA2SJcHr-cEv77_GKUOftrJLSDtB-_mosVkxzzItPwyUicfkHcolfDelqiwZMprKx2yzgwZ3I1E11JrW-aKWaV5gvioemDkEOHx7X1cfXx9sTl7u7r88Obd2avLlWYd61ZAGWWG0HUj10z3vVFc9bVSlIJReNGY4VqWbbRdA0WQQHEnsWqp4gRjdlw93_tOwX_JEJMYbdQwDNKBz1FQ1nLadm3TFfTZP-iVz8GV6QSta9qsa0YXwxd7quwlxgBGTMGOMsyCYLFUKJYKxZ8KC_z01jKrsah36F1nBSB74KsdYP6PldicXpzvTX8DG5utEw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2442574320</pqid></control><display><type>article</type><title>The immune enhancement effects of recombinant NDV expressing chicken granulocyte‐macrophage colony‐stimulating factor on the different avian influenza vaccine subtypes</title><source>Wiley Online Library All Journals</source><creator>Guo, Xiaochen ; Zhang, Teng ; Wang, Xiangxiang ; Su, Han ; Sun, Wenying ; Liu, Yunye ; Kang, Kai ; Liu, Tianyan ; Jiang, Shan ; Wang, Yaoqun ; Wang, Dan ; Yin, He ; Tian, Limin ; Li, Deshan ; Ren, Guiping</creator><creatorcontrib>Guo, Xiaochen ; Zhang, Teng ; Wang, Xiangxiang ; Su, Han ; Sun, Wenying ; Liu, Yunye ; Kang, Kai ; Liu, Tianyan ; Jiang, Shan ; Wang, Yaoqun ; Wang, Dan ; Yin, He ; Tian, Limin ; Li, Deshan ; Ren, Guiping</creatorcontrib><description>Avian influenza is an acute and highly contagious infectious disease that is caused by the influenza virus. Avian influenza has been widely spread all over the world, has caused property loss and has threatened human life and security. In this study, the recombinant plasmid rClone30‐chGM‐CSF was constructed and rescued to the recombinant virus rClone30‐chGM‐CSF successfully. After 8 days of immunization with the recombinant virus, the titre of NDV HI (haemagglutination inhibition) antibodies in SPF chickens reached its peak. The average titre of the rClone30‐chGM‐CSF group reached 6 log2 and was significantly higher than the protection critical value of 4 log2; the titres of the rClone30 group and the blank group were 2.86 log2 and 1 log2, respectively, indicating that the recombinant virus can effectively improve the NDV antibody titre. Then, SPF chickens were co‐immunized with the recombinant virus and with three different vaccine subtypes of inactivated avian influenza. The results indicated that the SPF chickens that were immunized with the vaccine plus rClone30‐chGM‐CSF showed significantly higher avian influenza antibody levels than those in the single vaccine groups. Furthermore, the SPF chickens in the vaccine plus rClone30‐chGM‐CSF group elicited stronger CD4+ and CD8+ T‐cell proliferative responses and also had upregulated transcriptional levels of interleukin‐1β (IL‐1β), IL‐4, IL‐6 and IL‐17 compared with those in the single vaccine groups. This study has shown that the recombinant virus expressing chicken granulocyte‐macrophage colony‐stimulating factor (chGM‐CSF) can be used not only as an NDV vaccine to effectively improve the titre of NDV antibodies but also as a biological adjuvant to enhance the immune effects of the avian influenza vaccine. Therefore, this recombinant virus can also be used as a biological adjuvant for other poultry vaccines.</description><identifier>ISSN: 1865-1674</identifier><identifier>EISSN: 1865-1682</identifier><identifier>DOI: 10.1111/tbed.13559</identifier><identifier>PMID: 32246561</identifier><language>eng</language><publisher>Germany: Hindawi Limited</publisher><subject>Antibodies ; Avian flu ; avian influenza ; CD4 antigen ; CD8 antigen ; Chickens ; Colonies ; Granulocytes ; Hemagglutination inhibition ; immune enhancement ; Immunization ; Infectious diseases ; Influenza ; Interleukins ; Leukocytes (granulocytic) ; Macrophages ; NDV ; Poultry ; Public health ; rClone30‐chGM‐CSF ; Security ; Transcription ; vaccine ; Vaccines ; Viruses</subject><ispartof>Transboundary and emerging diseases, 2020-09, Vol.67 (5), p.2108-2117</ispartof><rights>2020 Blackwell Verlag GmbH</rights><rights>2020 Blackwell Verlag GmbH.</rights><rights>Copyright © 2020 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3939-e2323f1275a73cddfb6bd4bb22efb0a73c3f6ca006895e0a7ae209a0b82b61003</citedby><cites>FETCH-LOGICAL-c3939-e2323f1275a73cddfb6bd4bb22efb0a73c3f6ca006895e0a7ae209a0b82b61003</cites><orcidid>0000-0003-2660-7137 ; 0000-0003-1859-5076 ; 0000-0001-8092-2408 ; 0000-0001-7683-5678 ; 0000-0001-6181-7100 ; 0000-0002-4787-4886 ; 0000-0001-8202-5380 ; 0000-0003-4251-4512 ; 0000-0002-9911-3026 ; 0000-0001-7733-2917 ; 0000-0002-1487-5276 ; 0000-0002-6877-6759 ; 0000-0001-5262-0584 ; 0000-0002-6214-6684 ; 0000-0001-8194-3542</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftbed.13559$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftbed.13559$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32246561$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Xiaochen</creatorcontrib><creatorcontrib>Zhang, Teng</creatorcontrib><creatorcontrib>Wang, Xiangxiang</creatorcontrib><creatorcontrib>Su, Han</creatorcontrib><creatorcontrib>Sun, Wenying</creatorcontrib><creatorcontrib>Liu, Yunye</creatorcontrib><creatorcontrib>Kang, Kai</creatorcontrib><creatorcontrib>Liu, Tianyan</creatorcontrib><creatorcontrib>Jiang, Shan</creatorcontrib><creatorcontrib>Wang, Yaoqun</creatorcontrib><creatorcontrib>Wang, Dan</creatorcontrib><creatorcontrib>Yin, He</creatorcontrib><creatorcontrib>Tian, Limin</creatorcontrib><creatorcontrib>Li, Deshan</creatorcontrib><creatorcontrib>Ren, Guiping</creatorcontrib><title>The immune enhancement effects of recombinant NDV expressing chicken granulocyte‐macrophage colony‐stimulating factor on the different avian influenza vaccine subtypes</title><title>Transboundary and emerging diseases</title><addtitle>Transbound Emerg Dis</addtitle><description>Avian influenza is an acute and highly contagious infectious disease that is caused by the influenza virus. Avian influenza has been widely spread all over the world, has caused property loss and has threatened human life and security. In this study, the recombinant plasmid rClone30‐chGM‐CSF was constructed and rescued to the recombinant virus rClone30‐chGM‐CSF successfully. After 8 days of immunization with the recombinant virus, the titre of NDV HI (haemagglutination inhibition) antibodies in SPF chickens reached its peak. The average titre of the rClone30‐chGM‐CSF group reached 6 log2 and was significantly higher than the protection critical value of 4 log2; the titres of the rClone30 group and the blank group were 2.86 log2 and 1 log2, respectively, indicating that the recombinant virus can effectively improve the NDV antibody titre. Then, SPF chickens were co‐immunized with the recombinant virus and with three different vaccine subtypes of inactivated avian influenza. The results indicated that the SPF chickens that were immunized with the vaccine plus rClone30‐chGM‐CSF showed significantly higher avian influenza antibody levels than those in the single vaccine groups. Furthermore, the SPF chickens in the vaccine plus rClone30‐chGM‐CSF group elicited stronger CD4+ and CD8+ T‐cell proliferative responses and also had upregulated transcriptional levels of interleukin‐1β (IL‐1β), IL‐4, IL‐6 and IL‐17 compared with those in the single vaccine groups. This study has shown that the recombinant virus expressing chicken granulocyte‐macrophage colony‐stimulating factor (chGM‐CSF) can be used not only as an NDV vaccine to effectively improve the titre of NDV antibodies but also as a biological adjuvant to enhance the immune effects of the avian influenza vaccine. Therefore, this recombinant virus can also be used as a biological adjuvant for other poultry vaccines.</description><subject>Antibodies</subject><subject>Avian flu</subject><subject>avian influenza</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Chickens</subject><subject>Colonies</subject><subject>Granulocytes</subject><subject>Hemagglutination inhibition</subject><subject>immune enhancement</subject><subject>Immunization</subject><subject>Infectious diseases</subject><subject>Influenza</subject><subject>Interleukins</subject><subject>Leukocytes (granulocytic)</subject><subject>Macrophages</subject><subject>NDV</subject><subject>Poultry</subject><subject>Public health</subject><subject>rClone30‐chGM‐CSF</subject><subject>Security</subject><subject>Transcription</subject><subject>vaccine</subject><subject>Vaccines</subject><subject>Viruses</subject><issn>1865-1674</issn><issn>1865-1682</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kUmO1DAYhSMEogfYcABkiQ1CqsZD4kqW9MAgtWBTsLVs53eVm8QOHhrSK47APbgVJ8GhulmwwBtbT5_f-_W_qnpC8Akp52VS0J8Q1jTdveqQtLxZEd7S-3_f6_qgOorxCmOOO948rA4YpTVvODmsfm52gOw4ZgcI3E46DSO4hMAY0Ckib1AA7UdlnSzy-_NPCL5NAWK0bov0zurP4NA2SJcHr-cEv77_GKUOftrJLSDtB-_mosVkxzzItPwyUicfkHcolfDelqiwZMprKx2yzgwZ3I1E11JrW-aKWaV5gvioemDkEOHx7X1cfXx9sTl7u7r88Obd2avLlWYd61ZAGWWG0HUj10z3vVFc9bVSlIJReNGY4VqWbbRdA0WQQHEnsWqp4gRjdlw93_tOwX_JEJMYbdQwDNKBz1FQ1nLadm3TFfTZP-iVz8GV6QSta9qsa0YXwxd7quwlxgBGTMGOMsyCYLFUKJYKxZ8KC_z01jKrsah36F1nBSB74KsdYP6PldicXpzvTX8DG5utEw</recordid><startdate>202009</startdate><enddate>202009</enddate><creator>Guo, Xiaochen</creator><creator>Zhang, Teng</creator><creator>Wang, Xiangxiang</creator><creator>Su, Han</creator><creator>Sun, Wenying</creator><creator>Liu, Yunye</creator><creator>Kang, Kai</creator><creator>Liu, Tianyan</creator><creator>Jiang, Shan</creator><creator>Wang, Yaoqun</creator><creator>Wang, Dan</creator><creator>Yin, He</creator><creator>Tian, Limin</creator><creator>Li, Deshan</creator><creator>Ren, Guiping</creator><general>Hindawi Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2660-7137</orcidid><orcidid>https://orcid.org/0000-0003-1859-5076</orcidid><orcidid>https://orcid.org/0000-0001-8092-2408</orcidid><orcidid>https://orcid.org/0000-0001-7683-5678</orcidid><orcidid>https://orcid.org/0000-0001-6181-7100</orcidid><orcidid>https://orcid.org/0000-0002-4787-4886</orcidid><orcidid>https://orcid.org/0000-0001-8202-5380</orcidid><orcidid>https://orcid.org/0000-0003-4251-4512</orcidid><orcidid>https://orcid.org/0000-0002-9911-3026</orcidid><orcidid>https://orcid.org/0000-0001-7733-2917</orcidid><orcidid>https://orcid.org/0000-0002-1487-5276</orcidid><orcidid>https://orcid.org/0000-0002-6877-6759</orcidid><orcidid>https://orcid.org/0000-0001-5262-0584</orcidid><orcidid>https://orcid.org/0000-0002-6214-6684</orcidid><orcidid>https://orcid.org/0000-0001-8194-3542</orcidid></search><sort><creationdate>202009</creationdate><title>The immune enhancement effects of recombinant NDV expressing chicken granulocyte‐macrophage colony‐stimulating factor on the different avian influenza vaccine subtypes</title><author>Guo, Xiaochen ; Zhang, Teng ; Wang, Xiangxiang ; Su, Han ; Sun, Wenying ; Liu, Yunye ; Kang, Kai ; Liu, Tianyan ; Jiang, Shan ; Wang, Yaoqun ; Wang, Dan ; Yin, He ; Tian, Limin ; Li, Deshan ; Ren, Guiping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3939-e2323f1275a73cddfb6bd4bb22efb0a73c3f6ca006895e0a7ae209a0b82b61003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibodies</topic><topic>Avian flu</topic><topic>avian influenza</topic><topic>CD4 antigen</topic><topic>CD8 antigen</topic><topic>Chickens</topic><topic>Colonies</topic><topic>Granulocytes</topic><topic>Hemagglutination inhibition</topic><topic>immune enhancement</topic><topic>Immunization</topic><topic>Infectious diseases</topic><topic>Influenza</topic><topic>Interleukins</topic><topic>Leukocytes (granulocytic)</topic><topic>Macrophages</topic><topic>NDV</topic><topic>Poultry</topic><topic>Public health</topic><topic>rClone30‐chGM‐CSF</topic><topic>Security</topic><topic>Transcription</topic><topic>vaccine</topic><topic>Vaccines</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Xiaochen</creatorcontrib><creatorcontrib>Zhang, Teng</creatorcontrib><creatorcontrib>Wang, Xiangxiang</creatorcontrib><creatorcontrib>Su, Han</creatorcontrib><creatorcontrib>Sun, Wenying</creatorcontrib><creatorcontrib>Liu, Yunye</creatorcontrib><creatorcontrib>Kang, Kai</creatorcontrib><creatorcontrib>Liu, Tianyan</creatorcontrib><creatorcontrib>Jiang, Shan</creatorcontrib><creatorcontrib>Wang, Yaoqun</creatorcontrib><creatorcontrib>Wang, Dan</creatorcontrib><creatorcontrib>Yin, He</creatorcontrib><creatorcontrib>Tian, Limin</creatorcontrib><creatorcontrib>Li, Deshan</creatorcontrib><creatorcontrib>Ren, Guiping</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transboundary and emerging diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Xiaochen</au><au>Zhang, Teng</au><au>Wang, Xiangxiang</au><au>Su, Han</au><au>Sun, Wenying</au><au>Liu, Yunye</au><au>Kang, Kai</au><au>Liu, Tianyan</au><au>Jiang, Shan</au><au>Wang, Yaoqun</au><au>Wang, Dan</au><au>Yin, He</au><au>Tian, Limin</au><au>Li, Deshan</au><au>Ren, Guiping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The immune enhancement effects of recombinant NDV expressing chicken granulocyte‐macrophage colony‐stimulating factor on the different avian influenza vaccine subtypes</atitle><jtitle>Transboundary and emerging diseases</jtitle><addtitle>Transbound Emerg Dis</addtitle><date>2020-09</date><risdate>2020</risdate><volume>67</volume><issue>5</issue><spage>2108</spage><epage>2117</epage><pages>2108-2117</pages><issn>1865-1674</issn><eissn>1865-1682</eissn><abstract>Avian influenza is an acute and highly contagious infectious disease that is caused by the influenza virus. Avian influenza has been widely spread all over the world, has caused property loss and has threatened human life and security. In this study, the recombinant plasmid rClone30‐chGM‐CSF was constructed and rescued to the recombinant virus rClone30‐chGM‐CSF successfully. After 8 days of immunization with the recombinant virus, the titre of NDV HI (haemagglutination inhibition) antibodies in SPF chickens reached its peak. The average titre of the rClone30‐chGM‐CSF group reached 6 log2 and was significantly higher than the protection critical value of 4 log2; the titres of the rClone30 group and the blank group were 2.86 log2 and 1 log2, respectively, indicating that the recombinant virus can effectively improve the NDV antibody titre. Then, SPF chickens were co‐immunized with the recombinant virus and with three different vaccine subtypes of inactivated avian influenza. The results indicated that the SPF chickens that were immunized with the vaccine plus rClone30‐chGM‐CSF showed significantly higher avian influenza antibody levels than those in the single vaccine groups. Furthermore, the SPF chickens in the vaccine plus rClone30‐chGM‐CSF group elicited stronger CD4+ and CD8+ T‐cell proliferative responses and also had upregulated transcriptional levels of interleukin‐1β (IL‐1β), IL‐4, IL‐6 and IL‐17 compared with those in the single vaccine groups. This study has shown that the recombinant virus expressing chicken granulocyte‐macrophage colony‐stimulating factor (chGM‐CSF) can be used not only as an NDV vaccine to effectively improve the titre of NDV antibodies but also as a biological adjuvant to enhance the immune effects of the avian influenza vaccine. 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| ispartof | Transboundary and emerging diseases, 2020-09, Vol.67 (5), p.2108-2117 |
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| source | Wiley Online Library All Journals |
| subjects | Antibodies Avian flu avian influenza CD4 antigen CD8 antigen Chickens Colonies Granulocytes Hemagglutination inhibition immune enhancement Immunization Infectious diseases Influenza Interleukins Leukocytes (granulocytic) Macrophages NDV Poultry Public health rClone30‐chGM‐CSF Security Transcription vaccine Vaccines Viruses |
| title | The immune enhancement effects of recombinant NDV expressing chicken granulocyte‐macrophage colony‐stimulating factor on the different avian influenza vaccine subtypes |
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