Optogenetic approaches for termination of ventricular tachyarrhythmias after myocardial infarction in rats in vivo
Cardiac optogenetics facilitates the painless manipulation of the heart with optical energy and was recently shown to terminate ventricular tachycardia (VT) in explanted mice heart. This study aimed to evaluate the optogenetic‐based termination of induced VT under ischemia in an open‐chest rat model...
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| Veröffentlicht in: | Journal of biophotonics 2020-07, Vol.13 (7), p.e202000003-n/a |
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| creator | Cheng, Yue Li, Haitao Wang, Long Li, Jianyi Kang, Wen Rao, Panpan Zhou, Fang Wang, Xi Huang, Congxin |
| description | Cardiac optogenetics facilitates the painless manipulation of the heart with optical energy and was recently shown to terminate ventricular tachycardia (VT) in explanted mice heart. This study aimed to evaluate the optogenetic‐based termination of induced VT under ischemia in an open‐chest rat model and to develop an optimal, optical‐manipulation procedure. VT was induced by burst stimulation after ligation of the left anterior descending coronary artery, and the termination effects of the optical manipulation, including electrical anti‐tachycardia pacing (ATP) and spontaneous recovery, were tested. Among different multisegment optical modes, four repeated illuminations of 1000 ms in duration with 1‐second interval at a 20‐times intensity threshold on the right ventricle achieved the highest termination rate of 86.14% ± 4.145%, higher than that achieved by ATP and spontaneous termination. We demonstrated that optogenetic‐based cardioversion is feasible and effective in vivo, with the underlying mechanism involving the light‐triggered, ChR2‐induced depolarization of the illuminated myocardium, in turn generating an excitation that disrupts the preexisting reentrant wave front.
Cardiac optogenetics facilitates the painless manipulation of the heart with optical energy and was recently shown to terminate ventricular tachycardia (VT) in explanted mice heart. This study aimed to evaluate the optogenetic‐based termination of induced VT under ischemia in an open‐chest rat model and to develop an optimal, optical‐manipulation procedure. VT was induced by burst stimulation after ligation of the left anterior descending coronary artery, and the termination effects of the optical manipulation, including electrical anti‐tachycardia pacing and spontaneous recovery, were tested. |
| doi_str_mv | 10.1002/jbio.202000003 |
| format | Article |
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Cardiac optogenetics facilitates the painless manipulation of the heart with optical energy and was recently shown to terminate ventricular tachycardia (VT) in explanted mice heart. This study aimed to evaluate the optogenetic‐based termination of induced VT under ischemia in an open‐chest rat model and to develop an optimal, optical‐manipulation procedure. VT was induced by burst stimulation after ligation of the left anterior descending coronary artery, and the termination effects of the optical manipulation, including electrical anti‐tachycardia pacing and spontaneous recovery, were tested.</description><identifier>ISSN: 1864-063X</identifier><identifier>EISSN: 1864-0648</identifier><identifier>DOI: 10.1002/jbio.202000003</identifier><identifier>PMID: 32246523</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag GmbH & Co. KGaA</publisher><subject>Cardiac arrhythmia ; channelrhodopsin‐2 ; Coronary artery ; defibrillation ; Depolarization ; Genetics ; Information processing ; Ischemia ; Myocardial infarction ; Myocardium ; Optics ; optogenetics ; Spontaneous recovery ; Tachycardia ; Ventricle ; ventricular tachyarrhythmia ; Wave fronts</subject><ispartof>Journal of biophotonics, 2020-07, Vol.13 (7), p.e202000003-n/a</ispartof><rights>2020 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3733-cf551866f38783ad6f6dfe03b07cf6c8d33d0336e9a6d87934bb0cb9df6763ff3</citedby><cites>FETCH-LOGICAL-c3733-cf551866f38783ad6f6dfe03b07cf6c8d33d0336e9a6d87934bb0cb9df6763ff3</cites><orcidid>0000-0002-4880-8442</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbio.202000003$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbio.202000003$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32246523$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Yue</creatorcontrib><creatorcontrib>Li, Haitao</creatorcontrib><creatorcontrib>Wang, Long</creatorcontrib><creatorcontrib>Li, Jianyi</creatorcontrib><creatorcontrib>Kang, Wen</creatorcontrib><creatorcontrib>Rao, Panpan</creatorcontrib><creatorcontrib>Zhou, Fang</creatorcontrib><creatorcontrib>Wang, Xi</creatorcontrib><creatorcontrib>Huang, Congxin</creatorcontrib><title>Optogenetic approaches for termination of ventricular tachyarrhythmias after myocardial infarction in rats in vivo</title><title>Journal of biophotonics</title><addtitle>J Biophotonics</addtitle><description>Cardiac optogenetics facilitates the painless manipulation of the heart with optical energy and was recently shown to terminate ventricular tachycardia (VT) in explanted mice heart. This study aimed to evaluate the optogenetic‐based termination of induced VT under ischemia in an open‐chest rat model and to develop an optimal, optical‐manipulation procedure. VT was induced by burst stimulation after ligation of the left anterior descending coronary artery, and the termination effects of the optical manipulation, including electrical anti‐tachycardia pacing (ATP) and spontaneous recovery, were tested. Among different multisegment optical modes, four repeated illuminations of 1000 ms in duration with 1‐second interval at a 20‐times intensity threshold on the right ventricle achieved the highest termination rate of 86.14% ± 4.145%, higher than that achieved by ATP and spontaneous termination. We demonstrated that optogenetic‐based cardioversion is feasible and effective in vivo, with the underlying mechanism involving the light‐triggered, ChR2‐induced depolarization of the illuminated myocardium, in turn generating an excitation that disrupts the preexisting reentrant wave front.
Cardiac optogenetics facilitates the painless manipulation of the heart with optical energy and was recently shown to terminate ventricular tachycardia (VT) in explanted mice heart. This study aimed to evaluate the optogenetic‐based termination of induced VT under ischemia in an open‐chest rat model and to develop an optimal, optical‐manipulation procedure. VT was induced by burst stimulation after ligation of the left anterior descending coronary artery, and the termination effects of the optical manipulation, including electrical anti‐tachycardia pacing and spontaneous recovery, were tested.</description><subject>Cardiac arrhythmia</subject><subject>channelrhodopsin‐2</subject><subject>Coronary artery</subject><subject>defibrillation</subject><subject>Depolarization</subject><subject>Genetics</subject><subject>Information processing</subject><subject>Ischemia</subject><subject>Myocardial infarction</subject><subject>Myocardium</subject><subject>Optics</subject><subject>optogenetics</subject><subject>Spontaneous recovery</subject><subject>Tachycardia</subject><subject>Ventricle</subject><subject>ventricular tachyarrhythmia</subject><subject>Wave fronts</subject><issn>1864-063X</issn><issn>1864-0648</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFkU1PwzAMhiMEYnxdOaJIXLhspHGbpkdAfGrSLiBxi9I0YZnaZiTtUP89GYMhccEXW_LjV7ZfhE4TMkkIoZeL0roJJZSsA3bQQcJZOiYs5bvbGl5H6DCEBSGMQAb7aASUpiyjcID8bNm5N93qziosl0vvpJrrgI3zuNO-sa3srGuxM3il285b1dcytiI1SO_nQzdvrAxYmkjjZnBK-srKGtvWSK--Zm2LvezCOq_syh2jPSProE--8xF6ubt9vnkYT2f3jzdX07GCHGCsTJbFA5gBnnOQFTOsMppASXJlmOIVQEUAmC4kq3heQFqWRJVFZVjOwBg4Qhcb3XjUe69DJxoblK5r2WrXB0GBM8qznLKInv9BF673bdxO0DQpGCEZ55GabCjlXQheG7H0tpF-EAkRazfE2g2xdSMOnH3L9mWjqy3-8_4IFBvgw9Z6-EdOPF0_zn7FPwFq8JjB</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Cheng, Yue</creator><creator>Li, Haitao</creator><creator>Wang, Long</creator><creator>Li, Jianyi</creator><creator>Kang, Wen</creator><creator>Rao, Panpan</creator><creator>Zhou, Fang</creator><creator>Wang, Xi</creator><creator>Huang, Congxin</creator><general>WILEY‐VCH Verlag GmbH & Co. KGaA</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SP</scope><scope>7SR</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>K9.</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4880-8442</orcidid></search><sort><creationdate>202007</creationdate><title>Optogenetic approaches for termination of ventricular tachyarrhythmias after myocardial infarction in rats in vivo</title><author>Cheng, Yue ; Li, Haitao ; Wang, Long ; Li, Jianyi ; Kang, Wen ; Rao, Panpan ; Zhou, Fang ; Wang, Xi ; Huang, Congxin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3733-cf551866f38783ad6f6dfe03b07cf6c8d33d0336e9a6d87934bb0cb9df6763ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Cardiac arrhythmia</topic><topic>channelrhodopsin‐2</topic><topic>Coronary artery</topic><topic>defibrillation</topic><topic>Depolarization</topic><topic>Genetics</topic><topic>Information processing</topic><topic>Ischemia</topic><topic>Myocardial infarction</topic><topic>Myocardium</topic><topic>Optics</topic><topic>optogenetics</topic><topic>Spontaneous recovery</topic><topic>Tachycardia</topic><topic>Ventricle</topic><topic>ventricular tachyarrhythmia</topic><topic>Wave fronts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Yue</creatorcontrib><creatorcontrib>Li, Haitao</creatorcontrib><creatorcontrib>Wang, Long</creatorcontrib><creatorcontrib>Li, Jianyi</creatorcontrib><creatorcontrib>Kang, Wen</creatorcontrib><creatorcontrib>Rao, Panpan</creatorcontrib><creatorcontrib>Zhou, Fang</creatorcontrib><creatorcontrib>Wang, Xi</creatorcontrib><creatorcontrib>Huang, Congxin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biophotonics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Yue</au><au>Li, Haitao</au><au>Wang, Long</au><au>Li, Jianyi</au><au>Kang, Wen</au><au>Rao, Panpan</au><au>Zhou, Fang</au><au>Wang, Xi</au><au>Huang, Congxin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optogenetic approaches for termination of ventricular tachyarrhythmias after myocardial infarction in rats in vivo</atitle><jtitle>Journal of biophotonics</jtitle><addtitle>J Biophotonics</addtitle><date>2020-07</date><risdate>2020</risdate><volume>13</volume><issue>7</issue><spage>e202000003</spage><epage>n/a</epage><pages>e202000003-n/a</pages><issn>1864-063X</issn><eissn>1864-0648</eissn><abstract>Cardiac optogenetics facilitates the painless manipulation of the heart with optical energy and was recently shown to terminate ventricular tachycardia (VT) in explanted mice heart. This study aimed to evaluate the optogenetic‐based termination of induced VT under ischemia in an open‐chest rat model and to develop an optimal, optical‐manipulation procedure. VT was induced by burst stimulation after ligation of the left anterior descending coronary artery, and the termination effects of the optical manipulation, including electrical anti‐tachycardia pacing (ATP) and spontaneous recovery, were tested. Among different multisegment optical modes, four repeated illuminations of 1000 ms in duration with 1‐second interval at a 20‐times intensity threshold on the right ventricle achieved the highest termination rate of 86.14% ± 4.145%, higher than that achieved by ATP and spontaneous termination. We demonstrated that optogenetic‐based cardioversion is feasible and effective in vivo, with the underlying mechanism involving the light‐triggered, ChR2‐induced depolarization of the illuminated myocardium, in turn generating an excitation that disrupts the preexisting reentrant wave front.
Cardiac optogenetics facilitates the painless manipulation of the heart with optical energy and was recently shown to terminate ventricular tachycardia (VT) in explanted mice heart. This study aimed to evaluate the optogenetic‐based termination of induced VT under ischemia in an open‐chest rat model and to develop an optimal, optical‐manipulation procedure. VT was induced by burst stimulation after ligation of the left anterior descending coronary artery, and the termination effects of the optical manipulation, including electrical anti‐tachycardia pacing and spontaneous recovery, were tested.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag GmbH & Co. KGaA</pub><pmid>32246523</pmid><doi>10.1002/jbio.202000003</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-4880-8442</orcidid></addata></record> |
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| subjects | Cardiac arrhythmia channelrhodopsin‐2 Coronary artery defibrillation Depolarization Genetics Information processing Ischemia Myocardial infarction Myocardium Optics optogenetics Spontaneous recovery Tachycardia Ventricle ventricular tachyarrhythmia Wave fronts |
| title | Optogenetic approaches for termination of ventricular tachyarrhythmias after myocardial infarction in rats in vivo |
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