Autophagy efficacy and vitamin D status: Population effects
•The efficacy of the autophagic response is shaped by 25(OH)D3, which is population specific.•1,25(OH)2D3 supplementation enhances autophagy under 25(OH)D3 deficient circumstances.•Supplementation under 25(OH)D3 sufficient circumstances does not benefit the autophagic response. Toll-like receptor (T...
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| Veröffentlicht in: | Cellular immunology 2020-06, Vol.352, p.104082-104082, Article 104082 |
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| creator | Abhimanyu Meyer, Vanessa Jones, Brandon R. Bornman, Liza |
| description | •The efficacy of the autophagic response is shaped by 25(OH)D3, which is population specific.•1,25(OH)2D3 supplementation enhances autophagy under 25(OH)D3 deficient circumstances.•Supplementation under 25(OH)D3 sufficient circumstances does not benefit the autophagic response.
Toll-like receptor (TLR) 2/1 signalling is linked to autophagy through transcriptional actions of the 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)-vitamin D receptor (VDR) complex. Population-specific effects have been reported for TLR2/1-VDR signalling. We hypothesized that population effects extend to autophagy and are influenced by vitamin D status. Serum 25(OH)D3 of healthy South Africans (Black individuals n = 10, White individuals n = 10) was quantified by LC-MS/MS. Primary monocytes-macrophages were supplemented in vitro with 1,25(OH)2D3 and stimulated with the lipoprotein Pam3CysSerLys4. TLR2, VDR, hCAP18, Beclin1, LC3-IIB, cytokines and CYP24A1 mRNA were quantified by flow cytometry and RT-qPCR, respectively. Black individuals showed significantly lower overall cumulative LC3-IIB (P |
| doi_str_mv | 10.1016/j.cellimm.2020.104082 |
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Toll-like receptor (TLR) 2/1 signalling is linked to autophagy through transcriptional actions of the 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)-vitamin D receptor (VDR) complex. Population-specific effects have been reported for TLR2/1-VDR signalling. We hypothesized that population effects extend to autophagy and are influenced by vitamin D status. Serum 25(OH)D3 of healthy South Africans (Black individuals n = 10, White individuals n = 10) was quantified by LC-MS/MS. Primary monocytes-macrophages were supplemented in vitro with 1,25(OH)2D3 and stimulated with the lipoprotein Pam3CysSerLys4. TLR2, VDR, hCAP18, Beclin1, LC3-IIB, cytokines and CYP24A1 mRNA were quantified by flow cytometry and RT-qPCR, respectively. Black individuals showed significantly lower overall cumulative LC3-IIB (P < 0.010), but higher Beclin1, VDR, IL6 and TNFA (P < 0.050) than White individuals. 1,25(OH)2D3 enhanced autophagic flux in monocytes-macrophages from Black individuals upon TLR2/1 stimulation and strengthened autophagy in 25(OH)D3 deficient individuals (independent cohort, n = 20). These findings support population-directed vitamin D supplementation.</description><identifier>ISSN: 0008-8749</identifier><identifier>EISSN: 1090-2163</identifier><identifier>DOI: 10.1016/j.cellimm.2020.104082</identifier><identifier>PMID: 32241530</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Adult ; African ; Autophagy ; Autophagy - drug effects ; Autophagy - physiology ; Beclin1 ; Calcitriol - blood ; Calcitriol - metabolism ; Calcitriol - physiology ; Chromatography, Liquid - methods ; Female ; Humans ; LC3-IIB ; Male ; Microtubule-Associated Proteins - genetics ; Middle Aged ; Monocytes - metabolism ; Pam3CSK4 ; Population-specific ; Primary Cell Culture ; Receptors, Calcitriol - genetics ; Receptors, Calcitriol - metabolism ; South Africa ; Tandem Mass Spectrometry - methods ; TLR2/1 ; Toll-Like Receptor 2 ; Toll-Like Receptors - genetics ; Toll-Like Receptors - metabolism ; VDR ; Vitamin D ; Vitamin D - metabolism ; Vitamin D - physiology</subject><ispartof>Cellular immunology, 2020-06, Vol.352, p.104082-104082, Article 104082</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-6b06bc5defe12285d092e2f55a8b3167047f4ee5215dcbd0aa3b2f5482108f613</citedby><cites>FETCH-LOGICAL-c365t-6b06bc5defe12285d092e2f55a8b3167047f4ee5215dcbd0aa3b2f5482108f613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0008874919304873$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32241530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abhimanyu</creatorcontrib><creatorcontrib>Meyer, Vanessa</creatorcontrib><creatorcontrib>Jones, Brandon R.</creatorcontrib><creatorcontrib>Bornman, Liza</creatorcontrib><title>Autophagy efficacy and vitamin D status: Population effects</title><title>Cellular immunology</title><addtitle>Cell Immunol</addtitle><description>•The efficacy of the autophagic response is shaped by 25(OH)D3, which is population specific.•1,25(OH)2D3 supplementation enhances autophagy under 25(OH)D3 deficient circumstances.•Supplementation under 25(OH)D3 sufficient circumstances does not benefit the autophagic response.
Toll-like receptor (TLR) 2/1 signalling is linked to autophagy through transcriptional actions of the 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)-vitamin D receptor (VDR) complex. Population-specific effects have been reported for TLR2/1-VDR signalling. We hypothesized that population effects extend to autophagy and are influenced by vitamin D status. Serum 25(OH)D3 of healthy South Africans (Black individuals n = 10, White individuals n = 10) was quantified by LC-MS/MS. Primary monocytes-macrophages were supplemented in vitro with 1,25(OH)2D3 and stimulated with the lipoprotein Pam3CysSerLys4. TLR2, VDR, hCAP18, Beclin1, LC3-IIB, cytokines and CYP24A1 mRNA were quantified by flow cytometry and RT-qPCR, respectively. Black individuals showed significantly lower overall cumulative LC3-IIB (P < 0.010), but higher Beclin1, VDR, IL6 and TNFA (P < 0.050) than White individuals. 1,25(OH)2D3 enhanced autophagic flux in monocytes-macrophages from Black individuals upon TLR2/1 stimulation and strengthened autophagy in 25(OH)D3 deficient individuals (independent cohort, n = 20). These findings support population-directed vitamin D supplementation.</description><subject>Adult</subject><subject>African</subject><subject>Autophagy</subject><subject>Autophagy - drug effects</subject><subject>Autophagy - physiology</subject><subject>Beclin1</subject><subject>Calcitriol - blood</subject><subject>Calcitriol - metabolism</subject><subject>Calcitriol - physiology</subject><subject>Chromatography, Liquid - methods</subject><subject>Female</subject><subject>Humans</subject><subject>LC3-IIB</subject><subject>Male</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Middle Aged</subject><subject>Monocytes - metabolism</subject><subject>Pam3CSK4</subject><subject>Population-specific</subject><subject>Primary Cell Culture</subject><subject>Receptors, Calcitriol - genetics</subject><subject>Receptors, Calcitriol - metabolism</subject><subject>South Africa</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>TLR2/1</subject><subject>Toll-Like Receptor 2</subject><subject>Toll-Like Receptors - genetics</subject><subject>Toll-Like Receptors - metabolism</subject><subject>VDR</subject><subject>Vitamin D</subject><subject>Vitamin D - metabolism</subject><subject>Vitamin D - physiology</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PwzAMhiMEgjH4CaAeuXQ4SdOmcEDT-JQmwQHOUZq4kKlfNO2k_XtadXDlZMl-bOt9CLmgsKBA4-vNwmBRuLJcMGBjLwLJDsiMQgohozE_JDMAkKFMovSEnHq_AaA0SuGYnHDGIio4zMjtsu_q5kt_7gLMc2e02QW6ssHWdbp0VXAf-E53vb8J3uqmL3Tn6mok0XT-jBzluvB4vq9z8vH48L56DtevTy-r5To0PBZdGGcQZ0ZYzJEyJoWFlCHLhdAy4zROIEryCFEwKqzJLGjNs2EcSUZB5jHlc3I13W3a-rtH36nS-TG9rrDuvWJcxixJOYyomFDT1t63mKumdaVud4qCGr2pjdp7U6M3NXkb9i73L_qsRPu39StqAO4mAIegW4et8sZhZdC6dnChbO3-efED-MF_4Q</recordid><startdate>202006</startdate><enddate>202006</enddate><creator>Abhimanyu</creator><creator>Meyer, Vanessa</creator><creator>Jones, Brandon R.</creator><creator>Bornman, Liza</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202006</creationdate><title>Autophagy efficacy and vitamin D status: Population effects</title><author>Abhimanyu ; Meyer, Vanessa ; Jones, Brandon R. ; Bornman, Liza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-6b06bc5defe12285d092e2f55a8b3167047f4ee5215dcbd0aa3b2f5482108f613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adult</topic><topic>African</topic><topic>Autophagy</topic><topic>Autophagy - drug effects</topic><topic>Autophagy - physiology</topic><topic>Beclin1</topic><topic>Calcitriol - blood</topic><topic>Calcitriol - metabolism</topic><topic>Calcitriol - physiology</topic><topic>Chromatography, Liquid - methods</topic><topic>Female</topic><topic>Humans</topic><topic>LC3-IIB</topic><topic>Male</topic><topic>Microtubule-Associated Proteins - genetics</topic><topic>Middle Aged</topic><topic>Monocytes - metabolism</topic><topic>Pam3CSK4</topic><topic>Population-specific</topic><topic>Primary Cell Culture</topic><topic>Receptors, Calcitriol - genetics</topic><topic>Receptors, Calcitriol - metabolism</topic><topic>South Africa</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>TLR2/1</topic><topic>Toll-Like Receptor 2</topic><topic>Toll-Like Receptors - genetics</topic><topic>Toll-Like Receptors - metabolism</topic><topic>VDR</topic><topic>Vitamin D</topic><topic>Vitamin D - metabolism</topic><topic>Vitamin D - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abhimanyu</creatorcontrib><creatorcontrib>Meyer, Vanessa</creatorcontrib><creatorcontrib>Jones, Brandon R.</creatorcontrib><creatorcontrib>Bornman, Liza</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abhimanyu</au><au>Meyer, Vanessa</au><au>Jones, Brandon R.</au><au>Bornman, Liza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autophagy efficacy and vitamin D status: Population effects</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>2020-06</date><risdate>2020</risdate><volume>352</volume><spage>104082</spage><epage>104082</epage><pages>104082-104082</pages><artnum>104082</artnum><issn>0008-8749</issn><eissn>1090-2163</eissn><abstract>•The efficacy of the autophagic response is shaped by 25(OH)D3, which is population specific.•1,25(OH)2D3 supplementation enhances autophagy under 25(OH)D3 deficient circumstances.•Supplementation under 25(OH)D3 sufficient circumstances does not benefit the autophagic response.
Toll-like receptor (TLR) 2/1 signalling is linked to autophagy through transcriptional actions of the 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)-vitamin D receptor (VDR) complex. Population-specific effects have been reported for TLR2/1-VDR signalling. We hypothesized that population effects extend to autophagy and are influenced by vitamin D status. Serum 25(OH)D3 of healthy South Africans (Black individuals n = 10, White individuals n = 10) was quantified by LC-MS/MS. Primary monocytes-macrophages were supplemented in vitro with 1,25(OH)2D3 and stimulated with the lipoprotein Pam3CysSerLys4. TLR2, VDR, hCAP18, Beclin1, LC3-IIB, cytokines and CYP24A1 mRNA were quantified by flow cytometry and RT-qPCR, respectively. Black individuals showed significantly lower overall cumulative LC3-IIB (P < 0.010), but higher Beclin1, VDR, IL6 and TNFA (P < 0.050) than White individuals. 1,25(OH)2D3 enhanced autophagic flux in monocytes-macrophages from Black individuals upon TLR2/1 stimulation and strengthened autophagy in 25(OH)D3 deficient individuals (independent cohort, n = 20). These findings support population-directed vitamin D supplementation.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>32241530</pmid><doi>10.1016/j.cellimm.2020.104082</doi><tpages>1</tpages></addata></record> |
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| subjects | Adult African Autophagy Autophagy - drug effects Autophagy - physiology Beclin1 Calcitriol - blood Calcitriol - metabolism Calcitriol - physiology Chromatography, Liquid - methods Female Humans LC3-IIB Male Microtubule-Associated Proteins - genetics Middle Aged Monocytes - metabolism Pam3CSK4 Population-specific Primary Cell Culture Receptors, Calcitriol - genetics Receptors, Calcitriol - metabolism South Africa Tandem Mass Spectrometry - methods TLR2/1 Toll-Like Receptor 2 Toll-Like Receptors - genetics Toll-Like Receptors - metabolism VDR Vitamin D Vitamin D - metabolism Vitamin D - physiology |
| title | Autophagy efficacy and vitamin D status: Population effects |
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