MiR-616 plays oncogenic role in hepatocellular carcinoma progression through suppressing PTEN expression and activating PI3K/AKT pathway
Given their regulatory roles in gene expression, microRNAs play an important role in tumorigenesis. The current study aimed to explore the function and the related mechanisms of in hepatocellular carcinoma (HCC). The expression of was detected using quantitative real-time polymerase chain reaction (...
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| Veröffentlicht in: | Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2020-12, Vol.48 (1), p.728-734 |
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| container_title | Artificial cells, nanomedicine, and biotechnology |
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| creator | Sa, Rina Song, Huali Wei, Meihua Su, Hailong Hong, Yao Zhou, Lulu Bai, Yunfei |
| description | Given their regulatory roles in gene expression, microRNAs play an important role in tumorigenesis. The current study aimed to explore the function and the related mechanisms of
in hepatocellular carcinoma (HCC).
The expression of
was detected using quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was applied to estimate the association of
with clinical characteristics of HCC patients. Cell transfection was performed by Lipofectamine
2000. MTT assay was used to detect cell proliferation, whereas cell motility was estimated using Transwell assay. The protein expression was detected using western blot.
was significantly up-regulated in HCC tissues and cells (
|
| doi_str_mv | 10.1080/21691401.2019.1596928 |
| format | Article |
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in hepatocellular carcinoma (HCC).
The expression of
was detected using quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was applied to estimate the association of
with clinical characteristics of HCC patients. Cell transfection was performed by Lipofectamine
2000. MTT assay was used to detect cell proliferation, whereas cell motility was estimated using Transwell assay. The protein expression was detected using western blot.
was significantly up-regulated in HCC tissues and cells (
< .05 for all). Moreover, its elevated expression was positively correlated with lymph node metastasis (
= .008) and TNM stage (
= .012). Knockdown of
resulted in decreased cell proliferation, migration and invasion. Moreover, the inhibition of
significantly suppressed PI3K/AKT pathway. The bioinformatics analysis and luciferase reporter assay suggested that
was a targeted gene of
.
had the capacity to reverse the oncogenic function of
in HCC.
activates PI3K/AKT pathway through suppressing
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in hepatocellular carcinoma (HCC).
The expression of
was detected using quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was applied to estimate the association of
with clinical characteristics of HCC patients. Cell transfection was performed by Lipofectamine
2000. MTT assay was used to detect cell proliferation, whereas cell motility was estimated using Transwell assay. The protein expression was detected using western blot.
was significantly up-regulated in HCC tissues and cells (
< .05 for all). Moreover, its elevated expression was positively correlated with lymph node metastasis (
= .008) and TNM stage (
= .012). Knockdown of
resulted in decreased cell proliferation, migration and invasion. Moreover, the inhibition of
significantly suppressed PI3K/AKT pathway. The bioinformatics analysis and luciferase reporter assay suggested that
was a targeted gene of
.
had the capacity to reverse the oncogenic function of
in HCC.
activates PI3K/AKT pathway through suppressing
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in hepatocellular carcinoma (HCC).
The expression of
was detected using quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was applied to estimate the association of
with clinical characteristics of HCC patients. Cell transfection was performed by Lipofectamine
2000. MTT assay was used to detect cell proliferation, whereas cell motility was estimated using Transwell assay. The protein expression was detected using western blot.
was significantly up-regulated in HCC tissues and cells (
< .05 for all). Moreover, its elevated expression was positively correlated with lymph node metastasis (
= .008) and TNM stage (
= .012). Knockdown of
resulted in decreased cell proliferation, migration and invasion. Moreover, the inhibition of
significantly suppressed PI3K/AKT pathway. The bioinformatics analysis and luciferase reporter assay suggested that
was a targeted gene of
.
had the capacity to reverse the oncogenic function of
in HCC.
activates PI3K/AKT pathway through suppressing
expression, thus promoting the progression of HCC.</abstract><cop>England</cop><pmid>32233811</pmid><doi>10.1080/21691401.2019.1596928</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Artificial cells, nanomedicine, and biotechnology, 2020-12, Vol.48 (1), p.728-734 |
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| language | eng |
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| source | DOAJ Directory of Open Access Journals; Access via Taylor & Francis (Open Access Collection); Alma/SFX Local Collection |
| title | MiR-616 plays oncogenic role in hepatocellular carcinoma progression through suppressing PTEN expression and activating PI3K/AKT pathway |
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