The mechanism of sublethal chlorantraniliprole exposure causing silkworm pupation metamorphosis defects
BACKGROUND Chlorantraniliprole (CAP) is widely used in agriculture and forestry to prevent and control pests. The effects of environmental CAP residue on non‐target insect metamorphosis have not been reported. Our research aimed to investigate the sublethal effect of CAP on larva–pupa transformation...
Gespeichert in:
| Veröffentlicht in: | Pest management science 2020-08, Vol.76 (8), p.2838-2845 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2845 |
|---|---|
| container_issue | 8 |
| container_start_page | 2838 |
| container_title | Pest management science |
| container_volume | 76 |
| creator | Chen, Jian Lu, Zhengting Li, Mengxue Mao, Tingting Wang, Hui Li, Fanchi Sun, Haina Dai, Minli Ye, Wentao Li, Bing |
| description | BACKGROUND
Chlorantraniliprole (CAP) is widely used in agriculture and forestry to prevent and control pests. The effects of environmental CAP residue on non‐target insect metamorphosis have not been reported. Our research aimed to investigate the sublethal effect of CAP on larva–pupa transformation in silkworm, and explore the mechanism of sublethal CAP exposure‐mediated pupation metamorphosis defects.
RESULT
Sublethal CAP exposure affected the growth and development of silkworm larvae and caused defects in pupation metamorphosis. After CAP exposure, formation the of prepupa procuticle, ecdysial membrane and new epidermis was inhibited. Also, the level of 20‐hydroxyecdysone (20E) and mRNA levels of the 20E signaling pathway‐related genes EcR, USP, E74, E75 and Ftz‐f1 were significantly reduced. Moreover, genes involved in chitin synthesis, such as ChsA, CDA1 and CDA2, were downregulated. Injection of 20E led to the upregulation of chitin synthesis‐related genes and increased formation of new epidermis in CAP‐treated silkworm. However, injection of 20E failed to prevent downregulation of Ftz‐f1 and the defects in pupation metamorphosis.
CONCLUSION
Our results suggested that 20E is a target hormone of CAP exposure‐mediated epidermis formation phenotype. Ftz‐f1 was silenced by CAP and might be a direct target gene of sublethal CAP exposure. Our study provided new evidence of the effects of sublethal CAP exposure on insect development and metamorphosis. © 2020 Society of Chemical Industry
Sublethal chlorantraniliprole (CAP) exposure causes pupation metamorphosis defects in silkworm. Our study suggests that CAP acts on the 20‐hydroxyecdysone (20E) signaling pathway and Ftz‐f1 is the target of CAP‐induced pupation failure.
© 2020 Society of Chemical Industry |
| doi_str_mv | 10.1002/ps.5836 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2385709343</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2385709343</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3456-5aa5099b40470713cf41da14255eb183ae78b1bed11d9ba9eb87a5cb02b4cf163</originalsourceid><addsrcrecordid>eNp1kE1rFjEURoMotr6K_0ACLizIW_M5H8tSrBYKCrbQXUgydzqpmcmYO0Pbf2_q23ZRcBFuFofDwyHkPWeHnDHxZcZD3cjqBdnnWlRb1bbNy6d_c7lH3iBeM8bathWvyZ4UQtZMin1ydT4AHcEPdgo40tRTXF2EZbCR-iGmbKelvBDDnFMECrdzwjUD9XbFMF1RDPH3TcojndfZLiFNxbbYMeV5SBiQdtCDX_AtedXbiPDu4W7IxcnX8-Pv27Mf306Pj862XipdbbW1uox0iqma1Vz6XvHOciW0BscbaaFuHHfQcd61zrbgmtpq75hwyve8khtysPOWuX9WwMWMAT3EaCdIKxohG12zVipZ0I_P0Ou05qmsM0IJrpisStMN-bSjfE6IGXoz5zDafGc4M_ftzYzmvn0hPzz4VjdC98Q9xi7A5x1wEyLc_c9jfv76p_sL8RiOaA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2421403658</pqid></control><display><type>article</type><title>The mechanism of sublethal chlorantraniliprole exposure causing silkworm pupation metamorphosis defects</title><source>Access via Wiley Online Library</source><creator>Chen, Jian ; Lu, Zhengting ; Li, Mengxue ; Mao, Tingting ; Wang, Hui ; Li, Fanchi ; Sun, Haina ; Dai, Minli ; Ye, Wentao ; Li, Bing</creator><creatorcontrib>Chen, Jian ; Lu, Zhengting ; Li, Mengxue ; Mao, Tingting ; Wang, Hui ; Li, Fanchi ; Sun, Haina ; Dai, Minli ; Ye, Wentao ; Li, Bing</creatorcontrib><description>BACKGROUND
Chlorantraniliprole (CAP) is widely used in agriculture and forestry to prevent and control pests. The effects of environmental CAP residue on non‐target insect metamorphosis have not been reported. Our research aimed to investigate the sublethal effect of CAP on larva–pupa transformation in silkworm, and explore the mechanism of sublethal CAP exposure‐mediated pupation metamorphosis defects.
RESULT
Sublethal CAP exposure affected the growth and development of silkworm larvae and caused defects in pupation metamorphosis. After CAP exposure, formation the of prepupa procuticle, ecdysial membrane and new epidermis was inhibited. Also, the level of 20‐hydroxyecdysone (20E) and mRNA levels of the 20E signaling pathway‐related genes EcR, USP, E74, E75 and Ftz‐f1 were significantly reduced. Moreover, genes involved in chitin synthesis, such as ChsA, CDA1 and CDA2, were downregulated. Injection of 20E led to the upregulation of chitin synthesis‐related genes and increased formation of new epidermis in CAP‐treated silkworm. However, injection of 20E failed to prevent downregulation of Ftz‐f1 and the defects in pupation metamorphosis.
CONCLUSION
Our results suggested that 20E is a target hormone of CAP exposure‐mediated epidermis formation phenotype. Ftz‐f1 was silenced by CAP and might be a direct target gene of sublethal CAP exposure. Our study provided new evidence of the effects of sublethal CAP exposure on insect development and metamorphosis. © 2020 Society of Chemical Industry
Sublethal chlorantraniliprole (CAP) exposure causes pupation metamorphosis defects in silkworm. Our study suggests that CAP acts on the 20‐hydroxyecdysone (20E) signaling pathway and Ftz‐f1 is the target of CAP‐induced pupation failure.
© 2020 Society of Chemical Industry</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.5836</identifier><identifier>PMID: 32237032</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>20E ; Bombyx mori ; Chitin ; chlorantraniliprole ; Defects ; Environmental effects ; Epidermis ; Exposure ; Forestry ; Ftz‐f1 ; Genes ; Injection ; Insecticides ; Insects ; Larvae ; Metamorphosis ; metamorphosis defects ; mRNA ; Pest control ; Pests ; Phenotypes ; Pupae ; Pupation ; Signal transduction ; Silkworms ; Synthesis</subject><ispartof>Pest management science, 2020-08, Vol.76 (8), p.2838-2845</ispartof><rights>2020 Society of Chemical Industry</rights><rights>2020 Society of Chemical Industry.</rights><rights>Copyright © 2020 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3456-5aa5099b40470713cf41da14255eb183ae78b1bed11d9ba9eb87a5cb02b4cf163</citedby><cites>FETCH-LOGICAL-c3456-5aa5099b40470713cf41da14255eb183ae78b1bed11d9ba9eb87a5cb02b4cf163</cites><orcidid>0000-0002-3331-5266 ; 0000-0002-8647-7533 ; 0000-0002-2072-2444 ; 0000-0003-3818-9817 ; 0000-0003-1315-4706 ; 0000-0001-6504-2728 ; 0000-0002-7640-9019 ; 0000-0002-5380-8359 ; 0000-0002-6554-2551 ; 0000-0002-1843-8081</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.5836$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.5836$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32237032$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Jian</creatorcontrib><creatorcontrib>Lu, Zhengting</creatorcontrib><creatorcontrib>Li, Mengxue</creatorcontrib><creatorcontrib>Mao, Tingting</creatorcontrib><creatorcontrib>Wang, Hui</creatorcontrib><creatorcontrib>Li, Fanchi</creatorcontrib><creatorcontrib>Sun, Haina</creatorcontrib><creatorcontrib>Dai, Minli</creatorcontrib><creatorcontrib>Ye, Wentao</creatorcontrib><creatorcontrib>Li, Bing</creatorcontrib><title>The mechanism of sublethal chlorantraniliprole exposure causing silkworm pupation metamorphosis defects</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
Chlorantraniliprole (CAP) is widely used in agriculture and forestry to prevent and control pests. The effects of environmental CAP residue on non‐target insect metamorphosis have not been reported. Our research aimed to investigate the sublethal effect of CAP on larva–pupa transformation in silkworm, and explore the mechanism of sublethal CAP exposure‐mediated pupation metamorphosis defects.
RESULT
Sublethal CAP exposure affected the growth and development of silkworm larvae and caused defects in pupation metamorphosis. After CAP exposure, formation the of prepupa procuticle, ecdysial membrane and new epidermis was inhibited. Also, the level of 20‐hydroxyecdysone (20E) and mRNA levels of the 20E signaling pathway‐related genes EcR, USP, E74, E75 and Ftz‐f1 were significantly reduced. Moreover, genes involved in chitin synthesis, such as ChsA, CDA1 and CDA2, were downregulated. Injection of 20E led to the upregulation of chitin synthesis‐related genes and increased formation of new epidermis in CAP‐treated silkworm. However, injection of 20E failed to prevent downregulation of Ftz‐f1 and the defects in pupation metamorphosis.
CONCLUSION
Our results suggested that 20E is a target hormone of CAP exposure‐mediated epidermis formation phenotype. Ftz‐f1 was silenced by CAP and might be a direct target gene of sublethal CAP exposure. Our study provided new evidence of the effects of sublethal CAP exposure on insect development and metamorphosis. © 2020 Society of Chemical Industry
Sublethal chlorantraniliprole (CAP) exposure causes pupation metamorphosis defects in silkworm. Our study suggests that CAP acts on the 20‐hydroxyecdysone (20E) signaling pathway and Ftz‐f1 is the target of CAP‐induced pupation failure.
© 2020 Society of Chemical Industry</description><subject>20E</subject><subject>Bombyx mori</subject><subject>Chitin</subject><subject>chlorantraniliprole</subject><subject>Defects</subject><subject>Environmental effects</subject><subject>Epidermis</subject><subject>Exposure</subject><subject>Forestry</subject><subject>Ftz‐f1</subject><subject>Genes</subject><subject>Injection</subject><subject>Insecticides</subject><subject>Insects</subject><subject>Larvae</subject><subject>Metamorphosis</subject><subject>metamorphosis defects</subject><subject>mRNA</subject><subject>Pest control</subject><subject>Pests</subject><subject>Phenotypes</subject><subject>Pupae</subject><subject>Pupation</subject><subject>Signal transduction</subject><subject>Silkworms</subject><subject>Synthesis</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kE1rFjEURoMotr6K_0ACLizIW_M5H8tSrBYKCrbQXUgydzqpmcmYO0Pbf2_q23ZRcBFuFofDwyHkPWeHnDHxZcZD3cjqBdnnWlRb1bbNy6d_c7lH3iBeM8bathWvyZ4UQtZMin1ydT4AHcEPdgo40tRTXF2EZbCR-iGmbKelvBDDnFMECrdzwjUD9XbFMF1RDPH3TcojndfZLiFNxbbYMeV5SBiQdtCDX_AtedXbiPDu4W7IxcnX8-Pv27Mf306Pj862XipdbbW1uox0iqma1Vz6XvHOciW0BscbaaFuHHfQcd61zrbgmtpq75hwyve8khtysPOWuX9WwMWMAT3EaCdIKxohG12zVipZ0I_P0Ou05qmsM0IJrpisStMN-bSjfE6IGXoz5zDafGc4M_ftzYzmvn0hPzz4VjdC98Q9xi7A5x1wEyLc_c9jfv76p_sL8RiOaA</recordid><startdate>202008</startdate><enddate>202008</enddate><creator>Chen, Jian</creator><creator>Lu, Zhengting</creator><creator>Li, Mengxue</creator><creator>Mao, Tingting</creator><creator>Wang, Hui</creator><creator>Li, Fanchi</creator><creator>Sun, Haina</creator><creator>Dai, Minli</creator><creator>Ye, Wentao</creator><creator>Li, Bing</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3331-5266</orcidid><orcidid>https://orcid.org/0000-0002-8647-7533</orcidid><orcidid>https://orcid.org/0000-0002-2072-2444</orcidid><orcidid>https://orcid.org/0000-0003-3818-9817</orcidid><orcidid>https://orcid.org/0000-0003-1315-4706</orcidid><orcidid>https://orcid.org/0000-0001-6504-2728</orcidid><orcidid>https://orcid.org/0000-0002-7640-9019</orcidid><orcidid>https://orcid.org/0000-0002-5380-8359</orcidid><orcidid>https://orcid.org/0000-0002-6554-2551</orcidid><orcidid>https://orcid.org/0000-0002-1843-8081</orcidid></search><sort><creationdate>202008</creationdate><title>The mechanism of sublethal chlorantraniliprole exposure causing silkworm pupation metamorphosis defects</title><author>Chen, Jian ; Lu, Zhengting ; Li, Mengxue ; Mao, Tingting ; Wang, Hui ; Li, Fanchi ; Sun, Haina ; Dai, Minli ; Ye, Wentao ; Li, Bing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3456-5aa5099b40470713cf41da14255eb183ae78b1bed11d9ba9eb87a5cb02b4cf163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>20E</topic><topic>Bombyx mori</topic><topic>Chitin</topic><topic>chlorantraniliprole</topic><topic>Defects</topic><topic>Environmental effects</topic><topic>Epidermis</topic><topic>Exposure</topic><topic>Forestry</topic><topic>Ftz‐f1</topic><topic>Genes</topic><topic>Injection</topic><topic>Insecticides</topic><topic>Insects</topic><topic>Larvae</topic><topic>Metamorphosis</topic><topic>metamorphosis defects</topic><topic>mRNA</topic><topic>Pest control</topic><topic>Pests</topic><topic>Phenotypes</topic><topic>Pupae</topic><topic>Pupation</topic><topic>Signal transduction</topic><topic>Silkworms</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Jian</creatorcontrib><creatorcontrib>Lu, Zhengting</creatorcontrib><creatorcontrib>Li, Mengxue</creatorcontrib><creatorcontrib>Mao, Tingting</creatorcontrib><creatorcontrib>Wang, Hui</creatorcontrib><creatorcontrib>Li, Fanchi</creatorcontrib><creatorcontrib>Sun, Haina</creatorcontrib><creatorcontrib>Dai, Minli</creatorcontrib><creatorcontrib>Ye, Wentao</creatorcontrib><creatorcontrib>Li, Bing</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Jian</au><au>Lu, Zhengting</au><au>Li, Mengxue</au><au>Mao, Tingting</au><au>Wang, Hui</au><au>Li, Fanchi</au><au>Sun, Haina</au><au>Dai, Minli</au><au>Ye, Wentao</au><au>Li, Bing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The mechanism of sublethal chlorantraniliprole exposure causing silkworm pupation metamorphosis defects</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2020-08</date><risdate>2020</risdate><volume>76</volume><issue>8</issue><spage>2838</spage><epage>2845</epage><pages>2838-2845</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
Chlorantraniliprole (CAP) is widely used in agriculture and forestry to prevent and control pests. The effects of environmental CAP residue on non‐target insect metamorphosis have not been reported. Our research aimed to investigate the sublethal effect of CAP on larva–pupa transformation in silkworm, and explore the mechanism of sublethal CAP exposure‐mediated pupation metamorphosis defects.
RESULT
Sublethal CAP exposure affected the growth and development of silkworm larvae and caused defects in pupation metamorphosis. After CAP exposure, formation the of prepupa procuticle, ecdysial membrane and new epidermis was inhibited. Also, the level of 20‐hydroxyecdysone (20E) and mRNA levels of the 20E signaling pathway‐related genes EcR, USP, E74, E75 and Ftz‐f1 were significantly reduced. Moreover, genes involved in chitin synthesis, such as ChsA, CDA1 and CDA2, were downregulated. Injection of 20E led to the upregulation of chitin synthesis‐related genes and increased formation of new epidermis in CAP‐treated silkworm. However, injection of 20E failed to prevent downregulation of Ftz‐f1 and the defects in pupation metamorphosis.
CONCLUSION
Our results suggested that 20E is a target hormone of CAP exposure‐mediated epidermis formation phenotype. Ftz‐f1 was silenced by CAP and might be a direct target gene of sublethal CAP exposure. Our study provided new evidence of the effects of sublethal CAP exposure on insect development and metamorphosis. © 2020 Society of Chemical Industry
Sublethal chlorantraniliprole (CAP) exposure causes pupation metamorphosis defects in silkworm. Our study suggests that CAP acts on the 20‐hydroxyecdysone (20E) signaling pathway and Ftz‐f1 is the target of CAP‐induced pupation failure.
© 2020 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>32237032</pmid><doi>10.1002/ps.5836</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-3331-5266</orcidid><orcidid>https://orcid.org/0000-0002-8647-7533</orcidid><orcidid>https://orcid.org/0000-0002-2072-2444</orcidid><orcidid>https://orcid.org/0000-0003-3818-9817</orcidid><orcidid>https://orcid.org/0000-0003-1315-4706</orcidid><orcidid>https://orcid.org/0000-0001-6504-2728</orcidid><orcidid>https://orcid.org/0000-0002-7640-9019</orcidid><orcidid>https://orcid.org/0000-0002-5380-8359</orcidid><orcidid>https://orcid.org/0000-0002-6554-2551</orcidid><orcidid>https://orcid.org/0000-0002-1843-8081</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1526-498X |
| ispartof | Pest management science, 2020-08, Vol.76 (8), p.2838-2845 |
| issn | 1526-498X 1526-4998 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2385709343 |
| source | Access via Wiley Online Library |
| subjects | 20E Bombyx mori Chitin chlorantraniliprole Defects Environmental effects Epidermis Exposure Forestry Ftz‐f1 Genes Injection Insecticides Insects Larvae Metamorphosis metamorphosis defects mRNA Pest control Pests Phenotypes Pupae Pupation Signal transduction Silkworms Synthesis |
| title | The mechanism of sublethal chlorantraniliprole exposure causing silkworm pupation metamorphosis defects |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T02%3A31%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20mechanism%20of%20sublethal%20chlorantraniliprole%20exposure%20causing%20silkworm%20pupation%20metamorphosis%20defects&rft.jtitle=Pest%20management%20science&rft.au=Chen,%20Jian&rft.date=2020-08&rft.volume=76&rft.issue=8&rft.spage=2838&rft.epage=2845&rft.pages=2838-2845&rft.issn=1526-498X&rft.eissn=1526-4998&rft_id=info:doi/10.1002/ps.5836&rft_dat=%3Cproquest_cross%3E2385709343%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2421403658&rft_id=info:pmid/32237032&rfr_iscdi=true |