High-Throughput Sequencing (HTS) of newly synthetized RNAs enables one shot detection and identification of live mycoplasmas and differentiation from inert nucleic acids

Mycoplasma contamination threatens both the safety of biologics produced in cell substrates as well as the quality of scientific results based on cell-culture observations. Methods currently used to detect contamination of cells include culture, enzymatic activity, immunofluorescence and PCR but suf...

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Veröffentlicht in:Biologicals 2020-05, Vol.65, p.18-24
Hauptverfasser: Desbrousses, Céline, Archer, Fabienne, Colin, Adélie, Bobet-Erny, Alexandra, Champavère, Angélique, Gros, Edwige, Beurdeley, Pascale, Cruveiller, Stéphane, Tardy, Florence, Eloit, Marc
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Sprache:eng
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