Development of an antigen-capture enzyme-linked immunosorbent assay and immunochromatographic strip based on monoclonal antibodies for detection of H6 avian influenza viruses
Continuous surveillance has shown that H6 subtype avian influenza viruses (AIVs) are prevalent in poultry and occasionally break the species barrier to infect humans. It is therefore necessary to establish a specific, rapid and sensitive method to screen H6 AIVs. In this study, a panel of monoclonal...
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| Veröffentlicht in: | Archives of virology 2020-05, Vol.165 (5), p.1129-1139 |
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| creator | Yang, Fan Xiao, Yixin Xu, Lihua Liu, Fumin Yao, Hangping Wu, Nanping Wu, Haibo |
| description | Continuous surveillance has shown that H6 subtype avian influenza viruses (AIVs) are prevalent in poultry and occasionally break the species barrier to infect humans. It is therefore necessary to establish a specific, rapid and sensitive method to screen H6 AIVs. In this study, a panel of monoclonal antibodies (mAbs) against the hemagglutinin (HA) of an H6 AIV isolate was produced. The purified mAbs have high affinity and specificity for H6 AIVs. An antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) and immunochromatographic strip were developed based on two mAbs (1D7 and 1F12). The AC-ELISA results showed high sensitivity with a limit of detection (LOD) of 3.9 ng/ml for H6 HA protein and 0.5 HAU (HA units)/100 µl for live H6 subtype AIVs. The average recovery of the AC-ELISA with allantoic fluid, respiratory specimens, and cloacal swabs was 91.907 ± 1.559%, 82.977 ± 1.497% and 73.791 ± 2.588%, respectively. The intra- and inter-assay coefficient of variation was less than 10%. The LOD of immunochromatographic strip was 1 HAU when evaluated by the naked eye, and the detection time was less than 10 min without any equipment. Storage at room temperature or 4 °C for 30 days or 60 days had no effect on sensitivity and specificity of the strip. Thus, the AC-ELISA and immunochromatographic strips described here could be a secondary method to diagnose H6 AIV infections with high specificity, sensitivity, and stability. |
| doi_str_mv | 10.1007/s00705-020-04602-w |
| format | Article |
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It is therefore necessary to establish a specific, rapid and sensitive method to screen H6 AIVs. In this study, a panel of monoclonal antibodies (mAbs) against the hemagglutinin (HA) of an H6 AIV isolate was produced. The purified mAbs have high affinity and specificity for H6 AIVs. An antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) and immunochromatographic strip were developed based on two mAbs (1D7 and 1F12). The AC-ELISA results showed high sensitivity with a limit of detection (LOD) of 3.9 ng/ml for H6 HA protein and 0.5 HAU (HA units)/100 µl for live H6 subtype AIVs. The average recovery of the AC-ELISA with allantoic fluid, respiratory specimens, and cloacal swabs was 91.907 ± 1.559%, 82.977 ± 1.497% and 73.791 ± 2.588%, respectively. The intra- and inter-assay coefficient of variation was less than 10%. The LOD of immunochromatographic strip was 1 HAU when evaluated by the naked eye, and the detection time was less than 10 min without any equipment. Storage at room temperature or 4 °C for 30 days or 60 days had no effect on sensitivity and specificity of the strip. Thus, the AC-ELISA and immunochromatographic strips described here could be a secondary method to diagnose H6 AIV infections with high specificity, sensitivity, and stability.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-020-04602-w</identifier><identifier>PMID: 32221715</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Allantoic fluid ; ambient temperature ; Animals ; Antibodies, Monoclonal - immunology ; Antibodies, Viral - immunology ; Antigens ; Avian flu ; avian influenza ; Biomedical and Life Sciences ; Biomedicine ; cloaca ; detection limit ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzymes ; HA protein ; Hemagglutinin Glycoproteins, Influenza Virus - immunology ; Hemagglutinins ; Humans ; immunoaffinity chromatography ; Immunoassay - methods ; Infectious Diseases ; Influenza ; Influenza A virus - immunology ; Influenza A virus - isolation & purification ; Influenza in Birds - diagnosis ; Influenza, Human - diagnosis ; Medical Microbiology ; monitoring ; Monoclonal antibodies ; Original Article ; Orthomyxoviridae ; Poultry ; Sensitivity and Specificity ; Virology</subject><ispartof>Archives of virology, 2020-05, Vol.165 (5), p.1129-1139</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-26f3cc5f628de4f7e3fc54db0ca842ea9c795b785be224a67c3d390828d86eb23</citedby><cites>FETCH-LOGICAL-c408t-26f3cc5f628de4f7e3fc54db0ca842ea9c795b785be224a67c3d390828d86eb23</cites><orcidid>0000-0001-5550-5186</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00705-020-04602-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00705-020-04602-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32221715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>Xiao, Yixin</creatorcontrib><creatorcontrib>Xu, Lihua</creatorcontrib><creatorcontrib>Liu, Fumin</creatorcontrib><creatorcontrib>Yao, Hangping</creatorcontrib><creatorcontrib>Wu, Nanping</creatorcontrib><creatorcontrib>Wu, Haibo</creatorcontrib><title>Development of an antigen-capture enzyme-linked immunosorbent assay and immunochromatographic strip based on monoclonal antibodies for detection of H6 avian influenza viruses</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><addtitle>Arch Virol</addtitle><description>Continuous surveillance has shown that H6 subtype avian influenza viruses (AIVs) are prevalent in poultry and occasionally break the species barrier to infect humans. It is therefore necessary to establish a specific, rapid and sensitive method to screen H6 AIVs. In this study, a panel of monoclonal antibodies (mAbs) against the hemagglutinin (HA) of an H6 AIV isolate was produced. The purified mAbs have high affinity and specificity for H6 AIVs. An antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) and immunochromatographic strip were developed based on two mAbs (1D7 and 1F12). The AC-ELISA results showed high sensitivity with a limit of detection (LOD) of 3.9 ng/ml for H6 HA protein and 0.5 HAU (HA units)/100 µl for live H6 subtype AIVs. The average recovery of the AC-ELISA with allantoic fluid, respiratory specimens, and cloacal swabs was 91.907 ± 1.559%, 82.977 ± 1.497% and 73.791 ± 2.588%, respectively. The intra- and inter-assay coefficient of variation was less than 10%. The LOD of immunochromatographic strip was 1 HAU when evaluated by the naked eye, and the detection time was less than 10 min without any equipment. Storage at room temperature or 4 °C for 30 days or 60 days had no effect on sensitivity and specificity of the strip. Thus, the AC-ELISA and immunochromatographic strips described here could be a secondary method to diagnose H6 AIV infections with high specificity, sensitivity, and stability.</description><subject>Allantoic fluid</subject><subject>ambient temperature</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigens</subject><subject>Avian flu</subject><subject>avian influenza</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>cloaca</subject><subject>detection limit</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzymes</subject><subject>HA protein</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - immunology</subject><subject>Hemagglutinins</subject><subject>Humans</subject><subject>immunoaffinity chromatography</subject><subject>Immunoassay - methods</subject><subject>Infectious Diseases</subject><subject>Influenza</subject><subject>Influenza A virus - immunology</subject><subject>Influenza A virus - isolation & purification</subject><subject>Influenza in Birds - diagnosis</subject><subject>Influenza, Human - diagnosis</subject><subject>Medical Microbiology</subject><subject>monitoring</subject><subject>Monoclonal antibodies</subject><subject>Original Article</subject><subject>Orthomyxoviridae</subject><subject>Poultry</subject><subject>Sensitivity and Specificity</subject><subject>Virology</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1u1DAUhS0EosPAC7BAltiwMdz4L84SlZ8iVWID68hxbqYuiR3sZKrhoXhGPJ0WJBYgWbZ0_Z17ru4h5HkFryuA-k0uFygGHBhIDZzdPCCbSgrOTN2Yh2QDAiQzGswZeZLzNUApCPWYnAnOeVVXakN-vsM9jnGeMCw0DtSGcha_w8CcnZc1IcXw4zAhG334hj3107SGmGPqjgqbsz0UxX3dXaU42SXukp2vvKN5SX6mnc1FGQOdYkHGGOx469LF3mOmQ0y0xwXd4gtThrjQ1O59GcWHYVyLv6V7n9aM-Sl5NNgx47O7d0u-fnj_5fyCXX7--On87SVzEszCuB6Ec2rQ3PQohxrF4JTsO3DWSI62cXWjutqoDjmXVtdO9KIBU3CjseNiS16d-s4pfl8xL-3ks8NxtAHjmluuVNVoAF39HxXFEiRoVdCXf6HXcU1lG0eqAVBcloi2hJ8ol2LOCYd2Tn6y6dBW0B6Db0_BtyX49jb49qaIXty1XrsJ-9-S-6QLIE5ALl9hh-mP9z_a_gIczL1s</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Yang, Fan</creator><creator>Xiao, Yixin</creator><creator>Xu, Lihua</creator><creator>Liu, Fumin</creator><creator>Yao, Hangping</creator><creator>Wu, Nanping</creator><creator>Wu, Haibo</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-5550-5186</orcidid></search><sort><creationdate>20200501</creationdate><title>Development of an antigen-capture enzyme-linked immunosorbent assay and immunochromatographic strip based on monoclonal antibodies for detection of H6 avian influenza viruses</title><author>Yang, Fan ; Xiao, Yixin ; Xu, Lihua ; Liu, Fumin ; Yao, Hangping ; Wu, Nanping ; Wu, Haibo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-26f3cc5f628de4f7e3fc54db0ca842ea9c795b785be224a67c3d390828d86eb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Allantoic fluid</topic><topic>ambient temperature</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Viral - immunology</topic><topic>Antigens</topic><topic>Avian flu</topic><topic>avian influenza</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>cloaca</topic><topic>detection limit</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzymes</topic><topic>HA protein</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - immunology</topic><topic>Hemagglutinins</topic><topic>Humans</topic><topic>immunoaffinity chromatography</topic><topic>Immunoassay - methods</topic><topic>Infectious Diseases</topic><topic>Influenza</topic><topic>Influenza A virus - immunology</topic><topic>Influenza A virus - isolation & purification</topic><topic>Influenza in Birds - diagnosis</topic><topic>Influenza, Human - diagnosis</topic><topic>Medical Microbiology</topic><topic>monitoring</topic><topic>Monoclonal antibodies</topic><topic>Original Article</topic><topic>Orthomyxoviridae</topic><topic>Poultry</topic><topic>Sensitivity and Specificity</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>Xiao, Yixin</creatorcontrib><creatorcontrib>Xu, Lihua</creatorcontrib><creatorcontrib>Liu, Fumin</creatorcontrib><creatorcontrib>Yao, Hangping</creatorcontrib><creatorcontrib>Wu, Nanping</creatorcontrib><creatorcontrib>Wu, Haibo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Fan</au><au>Xiao, Yixin</au><au>Xu, Lihua</au><au>Liu, Fumin</au><au>Yao, Hangping</au><au>Wu, Nanping</au><au>Wu, Haibo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an antigen-capture enzyme-linked immunosorbent assay and immunochromatographic strip based on monoclonal antibodies for detection of H6 avian influenza viruses</atitle><jtitle>Archives of virology</jtitle><stitle>Arch Virol</stitle><addtitle>Arch Virol</addtitle><date>2020-05-01</date><risdate>2020</risdate><volume>165</volume><issue>5</issue><spage>1129</spage><epage>1139</epage><pages>1129-1139</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>Continuous surveillance has shown that H6 subtype avian influenza viruses (AIVs) are prevalent in poultry and occasionally break the species barrier to infect humans. It is therefore necessary to establish a specific, rapid and sensitive method to screen H6 AIVs. In this study, a panel of monoclonal antibodies (mAbs) against the hemagglutinin (HA) of an H6 AIV isolate was produced. The purified mAbs have high affinity and specificity for H6 AIVs. An antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) and immunochromatographic strip were developed based on two mAbs (1D7 and 1F12). The AC-ELISA results showed high sensitivity with a limit of detection (LOD) of 3.9 ng/ml for H6 HA protein and 0.5 HAU (HA units)/100 µl for live H6 subtype AIVs. The average recovery of the AC-ELISA with allantoic fluid, respiratory specimens, and cloacal swabs was 91.907 ± 1.559%, 82.977 ± 1.497% and 73.791 ± 2.588%, respectively. The intra- and inter-assay coefficient of variation was less than 10%. The LOD of immunochromatographic strip was 1 HAU when evaluated by the naked eye, and the detection time was less than 10 min without any equipment. Storage at room temperature or 4 °C for 30 days or 60 days had no effect on sensitivity and specificity of the strip. Thus, the AC-ELISA and immunochromatographic strips described here could be a secondary method to diagnose H6 AIV infections with high specificity, sensitivity, and stability.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>32221715</pmid><doi>10.1007/s00705-020-04602-w</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-5550-5186</orcidid></addata></record> |
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| subjects | Allantoic fluid ambient temperature Animals Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Antigens Avian flu avian influenza Biomedical and Life Sciences Biomedicine cloaca detection limit Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzymes HA protein Hemagglutinin Glycoproteins, Influenza Virus - immunology Hemagglutinins Humans immunoaffinity chromatography Immunoassay - methods Infectious Diseases Influenza Influenza A virus - immunology Influenza A virus - isolation & purification Influenza in Birds - diagnosis Influenza, Human - diagnosis Medical Microbiology monitoring Monoclonal antibodies Original Article Orthomyxoviridae Poultry Sensitivity and Specificity Virology |
| title | Development of an antigen-capture enzyme-linked immunosorbent assay and immunochromatographic strip based on monoclonal antibodies for detection of H6 avian influenza viruses |
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