Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina)
Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina) was examined in this study. The pancreas is both an exocrine and endocrine gland with autonomic and sensory innervation presented by the numerous nerve fibers and small agglomerations of nerve cells. Inv...
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| description | Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina) was examined in this study. The pancreas is both an exocrine and endocrine gland with autonomic and sensory innervation presented by the numerous nerve fibers and small agglomerations of nerve cells.
Investigations were performed on 16 adult chinchillas of both sexes. The material was collected immediately after death of the animals. Histochemical methods: AChE and SPG were used, in addition to routine technique of single and double immunohistochemical (IHC) staining using whole mount specimens and freezing sections with a thickness of 8 to 12 μm. In the immunofluorescence staining, primary antibodies directed against markers used to identify cholinergic - ChAT and VAChT, and adrenergic - DbH and TH neurons. Secondary antibodies were coupled to Alexa Fluor 488 and Alexa Fluor 555 fluorophores.
Histochemical studies (AChE) revealed that chinchilla pancreatic cholinergic innervation consisted of ganglionic neurocytes and numerous nerve fibers. These structures are located in the parenchyma of the exocrine part of the organ in close proximity to blood vessels and are present within the walls of the pancreatic ducts and interstitial connective tissue. A delicate fiber network around the Langerhans islets was also observed. The most numerous cholinergic structures were found in the head and tail, and the least numbers were found in the body of the pancreas. The SPG method revealed that adrenergic fibers form a network in the adventitia of blood vessels, and individual fibers run throughout the pancreatic parenchyma. Moreover, adrenergic nerve fibers were observed around the ganglionic neurocytes. This innervation was similar in all parts of the investigated organ. IHC investigations allowed observations of both the cholinergic and adrenergic activities of autonomic nerve structures. Additionally, using ChAT/DbH double staining, colocalization of these substances was observed in the fibers of the pancreatic parenchyma that passed through the cholinergic ganglia. Colocalization of VAChT and TH was found in nerve fibers of the exocrine part, in the walls of blood vessels, and in individual nerve cells. Colocalization of ChAT/DbH and VAChT/TH was observed in the single nerve cells and in the small (2-3 cell) ganglia. ChAT- and DbH-immunopositive nerve fibers were found in the area of the islets of Langerhans.
The results indicate a more intense cholinergic innervation o |
| doi_str_mv | 10.5603/FHC.a2020.0005 |
| format | Article |
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Investigations were performed on 16 adult chinchillas of both sexes. The material was collected immediately after death of the animals. Histochemical methods: AChE and SPG were used, in addition to routine technique of single and double immunohistochemical (IHC) staining using whole mount specimens and freezing sections with a thickness of 8 to 12 μm. In the immunofluorescence staining, primary antibodies directed against markers used to identify cholinergic - ChAT and VAChT, and adrenergic - DbH and TH neurons. Secondary antibodies were coupled to Alexa Fluor 488 and Alexa Fluor 555 fluorophores.
Histochemical studies (AChE) revealed that chinchilla pancreatic cholinergic innervation consisted of ganglionic neurocytes and numerous nerve fibers. These structures are located in the parenchyma of the exocrine part of the organ in close proximity to blood vessels and are present within the walls of the pancreatic ducts and interstitial connective tissue. A delicate fiber network around the Langerhans islets was also observed. The most numerous cholinergic structures were found in the head and tail, and the least numbers were found in the body of the pancreas. The SPG method revealed that adrenergic fibers form a network in the adventitia of blood vessels, and individual fibers run throughout the pancreatic parenchyma. Moreover, adrenergic nerve fibers were observed around the ganglionic neurocytes. This innervation was similar in all parts of the investigated organ. IHC investigations allowed observations of both the cholinergic and adrenergic activities of autonomic nerve structures. Additionally, using ChAT/DbH double staining, colocalization of these substances was observed in the fibers of the pancreatic parenchyma that passed through the cholinergic ganglia. Colocalization of VAChT and TH was found in nerve fibers of the exocrine part, in the walls of blood vessels, and in individual nerve cells. Colocalization of ChAT/DbH and VAChT/TH was observed in the single nerve cells and in the small (2-3 cell) ganglia. ChAT- and DbH-immunopositive nerve fibers were found in the area of the islets of Langerhans.
The results indicate a more intense cholinergic innervation of the chinchilla's pancreas, which is represented by both ganglia and nerve fibers, while adrenergic structures are mainly represented by fibers and only single neurocytes. This arrangement of the investigated structures in this species may imply a major role for hormonal control of exocrine secretion in rodents.</description><identifier>ISSN: 0239-8508</identifier><identifier>EISSN: 1897-5631</identifier><identifier>DOI: 10.5603/FHC.a2020.0005</identifier><identifier>PMID: 32202307</identifier><language>eng</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Acetylcholinesterase ; Adrenergic nerves ; Antibodies ; Autonomic nervous system ; Blood vessels ; Chemical compounds ; Cholinergics ; Connective tissues ; Endocrine glands ; Fibers ; Fluorescence ; Fluorophores ; Freezing ; Ganglia ; Immunofluorescence ; Immunoglobulins ; Innervation ; Investigations ; Islets of Langerhans ; Laboratory animals ; Methods ; Nerves ; Pancreas ; Parenchyma ; Potassium ; Staining ; Sucrose</subject><ispartof>Folia histochemica et cytobiologica, 2020-01, Vol.1 (1), p.54-60</ispartof><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-b4d597552bbc5943a80b477da231a0c8a51922f448d1418b8e6ad3e15e15cf813</citedby><orcidid>0000-0003-4580-0565 ; 0000-0001-8517-7160 ; 0000-0001-6627-5347 ; 0000-0002-5450-922X ; 0000-0003-4024-0140</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32202307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Radzimirska, Malgorzata</creatorcontrib><creatorcontrib>Kuchinka, Jacek</creatorcontrib><creatorcontrib>Nowak, Elzbieta</creatorcontrib><creatorcontrib>Trybus, Wojciech</creatorcontrib><creatorcontrib>Szczurkowski, Aleksander</creatorcontrib><title>Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina)</title><title>Folia histochemica et cytobiologica</title><addtitle>Folia Histochem Cytobiol</addtitle><description>Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina) was examined in this study. The pancreas is both an exocrine and endocrine gland with autonomic and sensory innervation presented by the numerous nerve fibers and small agglomerations of nerve cells.
Investigations were performed on 16 adult chinchillas of both sexes. The material was collected immediately after death of the animals. Histochemical methods: AChE and SPG were used, in addition to routine technique of single and double immunohistochemical (IHC) staining using whole mount specimens and freezing sections with a thickness of 8 to 12 μm. In the immunofluorescence staining, primary antibodies directed against markers used to identify cholinergic - ChAT and VAChT, and adrenergic - DbH and TH neurons. Secondary antibodies were coupled to Alexa Fluor 488 and Alexa Fluor 555 fluorophores.
Histochemical studies (AChE) revealed that chinchilla pancreatic cholinergic innervation consisted of ganglionic neurocytes and numerous nerve fibers. These structures are located in the parenchyma of the exocrine part of the organ in close proximity to blood vessels and are present within the walls of the pancreatic ducts and interstitial connective tissue. A delicate fiber network around the Langerhans islets was also observed. The most numerous cholinergic structures were found in the head and tail, and the least numbers were found in the body of the pancreas. The SPG method revealed that adrenergic fibers form a network in the adventitia of blood vessels, and individual fibers run throughout the pancreatic parenchyma. Moreover, adrenergic nerve fibers were observed around the ganglionic neurocytes. This innervation was similar in all parts of the investigated organ. IHC investigations allowed observations of both the cholinergic and adrenergic activities of autonomic nerve structures. Additionally, using ChAT/DbH double staining, colocalization of these substances was observed in the fibers of the pancreatic parenchyma that passed through the cholinergic ganglia. Colocalization of VAChT and TH was found in nerve fibers of the exocrine part, in the walls of blood vessels, and in individual nerve cells. Colocalization of ChAT/DbH and VAChT/TH was observed in the single nerve cells and in the small (2-3 cell) ganglia. ChAT- and DbH-immunopositive nerve fibers were found in the area of the islets of Langerhans.
The results indicate a more intense cholinergic innervation of the chinchilla's pancreas, which is represented by both ganglia and nerve fibers, while adrenergic structures are mainly represented by fibers and only single neurocytes. This arrangement of the investigated structures in this species may imply a major role for hormonal control of exocrine secretion in rodents.</description><subject>Acetylcholinesterase</subject><subject>Adrenergic nerves</subject><subject>Antibodies</subject><subject>Autonomic nervous system</subject><subject>Blood vessels</subject><subject>Chemical compounds</subject><subject>Cholinergics</subject><subject>Connective tissues</subject><subject>Endocrine glands</subject><subject>Fibers</subject><subject>Fluorescence</subject><subject>Fluorophores</subject><subject>Freezing</subject><subject>Ganglia</subject><subject>Immunofluorescence</subject><subject>Immunoglobulins</subject><subject>Innervation</subject><subject>Investigations</subject><subject>Islets of Langerhans</subject><subject>Laboratory 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and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina)</title><author>Radzimirska, Malgorzata ; Kuchinka, Jacek ; Nowak, Elzbieta ; Trybus, Wojciech ; Szczurkowski, Aleksander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-b4d597552bbc5943a80b477da231a0c8a51922f448d1418b8e6ad3e15e15cf813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acetylcholinesterase</topic><topic>Adrenergic nerves</topic><topic>Antibodies</topic><topic>Autonomic nervous system</topic><topic>Blood vessels</topic><topic>Chemical compounds</topic><topic>Cholinergics</topic><topic>Connective tissues</topic><topic>Endocrine 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Radzimirska, Malgorzata</au><au>Kuchinka, Jacek</au><au>Nowak, Elzbieta</au><au>Trybus, Wojciech</au><au>Szczurkowski, Aleksander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina)</atitle><jtitle>Folia histochemica et cytobiologica</jtitle><addtitle>Folia Histochem Cytobiol</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>1</volume><issue>1</issue><spage>54</spage><epage>60</epage><pages>54-60</pages><issn>0239-8508</issn><eissn>1897-5631</eissn><abstract>Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina) was examined in this study. The pancreas is both an exocrine and endocrine gland with autonomic and sensory innervation presented by the numerous nerve fibers and small agglomerations of nerve cells.
Investigations were performed on 16 adult chinchillas of both sexes. The material was collected immediately after death of the animals. Histochemical methods: AChE and SPG were used, in addition to routine technique of single and double immunohistochemical (IHC) staining using whole mount specimens and freezing sections with a thickness of 8 to 12 μm. In the immunofluorescence staining, primary antibodies directed against markers used to identify cholinergic - ChAT and VAChT, and adrenergic - DbH and TH neurons. Secondary antibodies were coupled to Alexa Fluor 488 and Alexa Fluor 555 fluorophores.
Histochemical studies (AChE) revealed that chinchilla pancreatic cholinergic innervation consisted of ganglionic neurocytes and numerous nerve fibers. These structures are located in the parenchyma of the exocrine part of the organ in close proximity to blood vessels and are present within the walls of the pancreatic ducts and interstitial connective tissue. A delicate fiber network around the Langerhans islets was also observed. The most numerous cholinergic structures were found in the head and tail, and the least numbers were found in the body of the pancreas. The SPG method revealed that adrenergic fibers form a network in the adventitia of blood vessels, and individual fibers run throughout the pancreatic parenchyma. Moreover, adrenergic nerve fibers were observed around the ganglionic neurocytes. This innervation was similar in all parts of the investigated organ. IHC investigations allowed observations of both the cholinergic and adrenergic activities of autonomic nerve structures. Additionally, using ChAT/DbH double staining, colocalization of these substances was observed in the fibers of the pancreatic parenchyma that passed through the cholinergic ganglia. Colocalization of VAChT and TH was found in nerve fibers of the exocrine part, in the walls of blood vessels, and in individual nerve cells. Colocalization of ChAT/DbH and VAChT/TH was observed in the single nerve cells and in the small (2-3 cell) ganglia. ChAT- and DbH-immunopositive nerve fibers were found in the area of the islets of Langerhans.
The results indicate a more intense cholinergic innervation of the chinchilla's pancreas, which is represented by both ganglia and nerve fibers, while adrenergic structures are mainly represented by fibers and only single neurocytes. This arrangement of the investigated structures in this species may imply a major role for hormonal control of exocrine secretion in rodents.</abstract><cop>Poland</cop><pub>Wydawnictwo Via Medica</pub><pmid>32202307</pmid><doi>10.5603/FHC.a2020.0005</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-4580-0565</orcidid><orcidid>https://orcid.org/0000-0001-8517-7160</orcidid><orcidid>https://orcid.org/0000-0001-6627-5347</orcidid><orcidid>https://orcid.org/0000-0002-5450-922X</orcidid><orcidid>https://orcid.org/0000-0003-4024-0140</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylcholinesterase Adrenergic nerves Antibodies Autonomic nervous system Blood vessels Chemical compounds Cholinergics Connective tissues Endocrine glands Fibers Fluorescence Fluorophores Freezing Ganglia Immunofluorescence Immunoglobulins Innervation Investigations Islets of Langerhans Laboratory animals Methods Nerves Pancreas Parenchyma Potassium Staining Sucrose |
| title | Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina) |
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