Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation
Spinal cord injury (SCI) is the destruction of spinal cord motor and sensory resulted from an attack on the spinal cord, which can cause significant physiological damage. The inflammasome is a multiprotein oligomer resulting in inflammation; the NLRP3 inflammasome composed of NLRP3, apoptosis-associ...
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| Veröffentlicht in: | Metabolic brain disease 2020-06, Vol.35 (5), p.809-818 |
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| creator | Mohammed, Ibrahim Ijaz, Sahar Mokhtari, Tahmineh Gholaminejhad, Morteza Mahdavipour, Marzieh Jameie, Behnamedin Akbari, Mohammad Hassanzadeh, Gholamreza |
| description | Spinal cord injury (SCI) is the destruction of spinal cord motor and sensory resulted from an attack on the spinal cord, which can cause significant physiological damage. The inflammasome is a multiprotein oligomer resulting in inflammation; the NLRP3 inflammasome composed of NLRP3, apoptosis-associated speck-like protein (ASC), procaspase-1, and cleavage of procaspase-1 into caspase-1 initiates the inflammatory response. Subventricular Zone (SVZ) is the origin of neural stem/progenitor cells (NS/PCs) in the adult brain. Extracellular vesicles (EVs) are tiny lipid membrane bilayer vesicles secreted by different types of cells playing an important role in cell-cell communications. The aim of this study was to investigate the effect of intrathecal transplantation of EVs on the NLRP3 inflammasome formation in SCI rats. Male wistar rats were divided into three groups as following: laminectotomy group, SCI group, and EVs group. EVs was isolated from SVZ, and characterized by western blot and DLS, and then injected into the SCI rats. Real-time PCR and western blot were carried out for gene expression and protein level of NLRP3, ASC, and Caspase-1. H&E and cresyl violet staining were performed for histological analyses, as well as BBB test for motor function. The results indicated high level in mRNA and protein level in SCI group in comparison with laminectomy (
p
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| doi_str_mv | 10.1007/s11011-020-00563-w |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2378880074</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2378880074</sourcerecordid><originalsourceid>FETCH-LOGICAL-c424t-ad1b0bbd664e9f8794ed94b75cd03d6c52a14cde9fa28b2fe60051e649fc51f43</originalsourceid><addsrcrecordid>eNp9kctuFDEQRS0EIpPAD7BAltiwafCzH0sUBYI0AsRjbflRBo_a7cHuniT8EL-JOxNAYsHKUt1zy6p7EXpCyQtKSPeyUEoobQgjDSGy5c3VPbShsuNNx1t5H21I38umEwM5Qael7AghXNLhITrhjPZSDnyDfn5azAGmOQe7jDrjH2mCxkEOB3AYruesLYzjrXSAEuwIBe9zimkG7JfJziFNesQZbDpAvsFhwlnPOCYHI04el31YdZuyq9puqYhZqW_BhNW7Mu-2Hz_wOvOjjlGXFKHycT_CNfYpR71yj9ADr8cCj-_eM_Tl9cXn88tm-_7N2_NX28YKJuZGO2qIMa5tBQy-7wYBbhCmk9YR7lormabCuqpp1hvmoa3JUWjF4K2kXvAz9Py4tx75fYEyqxjKGoGeIC1FMd71fV_TX9Fn_6C7tOR6baUEYUww1vJKsSNlcyolg1f7HKLON4oStdaojjWqWqO6rVFdVdPTu9WLieD-WH73VgF-BEqVpq-Q__79n7W_AKwxrV4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2402242263</pqid></control><display><type>article</type><title>Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation</title><source>SpringerLink Journals</source><creator>Mohammed, Ibrahim ; Ijaz, Sahar ; Mokhtari, Tahmineh ; Gholaminejhad, Morteza ; Mahdavipour, Marzieh ; Jameie, Behnamedin ; Akbari, Mohammad ; Hassanzadeh, Gholamreza</creator><creatorcontrib>Mohammed, Ibrahim ; Ijaz, Sahar ; Mokhtari, Tahmineh ; Gholaminejhad, Morteza ; Mahdavipour, Marzieh ; Jameie, Behnamedin ; Akbari, Mohammad ; Hassanzadeh, Gholamreza</creatorcontrib><description>Spinal cord injury (SCI) is the destruction of spinal cord motor and sensory resulted from an attack on the spinal cord, which can cause significant physiological damage. The inflammasome is a multiprotein oligomer resulting in inflammation; the NLRP3 inflammasome composed of NLRP3, apoptosis-associated speck-like protein (ASC), procaspase-1, and cleavage of procaspase-1 into caspase-1 initiates the inflammatory response. Subventricular Zone (SVZ) is the origin of neural stem/progenitor cells (NS/PCs) in the adult brain. Extracellular vesicles (EVs) are tiny lipid membrane bilayer vesicles secreted by different types of cells playing an important role in cell-cell communications. The aim of this study was to investigate the effect of intrathecal transplantation of EVs on the NLRP3 inflammasome formation in SCI rats. Male wistar rats were divided into three groups as following: laminectotomy group, SCI group, and EVs group. EVs was isolated from SVZ, and characterized by western blot and DLS, and then injected into the SCI rats. Real-time PCR and western blot were carried out for gene expression and protein level of NLRP3, ASC, and Caspase-1. H&E and cresyl violet staining were performed for histological analyses, as well as BBB test for motor function. The results indicated high level in mRNA and protein level in SCI group in comparison with laminectomy (
p
< 0.001), and injection of EVs showed a significant reduction in the mRNA and protein levels in EVs group compared to SCI (
p
< 0.001). H&E and cresyl violet staining showed recovery in neural cells of spinal cord tissue in EVs group in comparison with SCI group. BBB test showed the promotion of motor function in EVs group compared to SCI in 14 days (
p
< 0.05). We concluded that the injection of EVs could recover the motor function in rats with SCI and rescue the neural cells of spinal cord tissue by suppressing the formation of the NLRP3 inflammasome complex.</description><identifier>ISSN: 0885-7490</identifier><identifier>EISSN: 1573-7365</identifier><identifier>DOI: 10.1007/s11011-020-00563-w</identifier><identifier>PMID: 32185593</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animal models ; Apoptosis ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Blood-brain barrier ; Caspase-1 ; Cells (biology) ; Complex formation ; Extracellular vesicles ; Gene expression ; Inflammasomes ; Inflammation ; Inflammatory response ; Injection ; Lipids ; Membrane vesicles ; Metabolic Diseases ; Neural stem cells ; Neurology ; Neurosciences ; Oncology ; Original Article ; Progenitor cells ; Proteins ; Recovery ; Recovery of function ; Rodents ; Spinal cord injuries ; Staining ; Subventricular zone ; Transplantation ; Vesicles</subject><ispartof>Metabolic brain disease, 2020-06, Vol.35 (5), p.809-818</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-ad1b0bbd664e9f8794ed94b75cd03d6c52a14cde9fa28b2fe60051e649fc51f43</citedby><cites>FETCH-LOGICAL-c424t-ad1b0bbd664e9f8794ed94b75cd03d6c52a14cde9fa28b2fe60051e649fc51f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11011-020-00563-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11011-020-00563-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32185593$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mohammed, Ibrahim</creatorcontrib><creatorcontrib>Ijaz, Sahar</creatorcontrib><creatorcontrib>Mokhtari, Tahmineh</creatorcontrib><creatorcontrib>Gholaminejhad, Morteza</creatorcontrib><creatorcontrib>Mahdavipour, Marzieh</creatorcontrib><creatorcontrib>Jameie, Behnamedin</creatorcontrib><creatorcontrib>Akbari, Mohammad</creatorcontrib><creatorcontrib>Hassanzadeh, Gholamreza</creatorcontrib><title>Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation</title><title>Metabolic brain disease</title><addtitle>Metab Brain Dis</addtitle><addtitle>Metab Brain Dis</addtitle><description>Spinal cord injury (SCI) is the destruction of spinal cord motor and sensory resulted from an attack on the spinal cord, which can cause significant physiological damage. The inflammasome is a multiprotein oligomer resulting in inflammation; the NLRP3 inflammasome composed of NLRP3, apoptosis-associated speck-like protein (ASC), procaspase-1, and cleavage of procaspase-1 into caspase-1 initiates the inflammatory response. Subventricular Zone (SVZ) is the origin of neural stem/progenitor cells (NS/PCs) in the adult brain. Extracellular vesicles (EVs) are tiny lipid membrane bilayer vesicles secreted by different types of cells playing an important role in cell-cell communications. The aim of this study was to investigate the effect of intrathecal transplantation of EVs on the NLRP3 inflammasome formation in SCI rats. Male wistar rats were divided into three groups as following: laminectotomy group, SCI group, and EVs group. EVs was isolated from SVZ, and characterized by western blot and DLS, and then injected into the SCI rats. Real-time PCR and western blot were carried out for gene expression and protein level of NLRP3, ASC, and Caspase-1. H&E and cresyl violet staining were performed for histological analyses, as well as BBB test for motor function. The results indicated high level in mRNA and protein level in SCI group in comparison with laminectomy (
p
< 0.001), and injection of EVs showed a significant reduction in the mRNA and protein levels in EVs group compared to SCI (
p
< 0.001). H&E and cresyl violet staining showed recovery in neural cells of spinal cord tissue in EVs group in comparison with SCI group. BBB test showed the promotion of motor function in EVs group compared to SCI in 14 days (
p
< 0.05). We concluded that the injection of EVs could recover the motor function in rats with SCI and rescue the neural cells of spinal cord tissue by suppressing the formation of the NLRP3 inflammasome complex.</description><subject>Animal models</subject><subject>Apoptosis</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blood-brain barrier</subject><subject>Caspase-1</subject><subject>Cells (biology)</subject><subject>Complex formation</subject><subject>Extracellular vesicles</subject><subject>Gene expression</subject><subject>Inflammasomes</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Injection</subject><subject>Lipids</subject><subject>Membrane vesicles</subject><subject>Metabolic Diseases</subject><subject>Neural stem cells</subject><subject>Neurology</subject><subject>Neurosciences</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Progenitor cells</subject><subject>Proteins</subject><subject>Recovery</subject><subject>Recovery of function</subject><subject>Rodents</subject><subject>Spinal cord injuries</subject><subject>Staining</subject><subject>Subventricular zone</subject><subject>Transplantation</subject><subject>Vesicles</subject><issn>0885-7490</issn><issn>1573-7365</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kctuFDEQRS0EIpPAD7BAltiwafCzH0sUBYI0AsRjbflRBo_a7cHuniT8EL-JOxNAYsHKUt1zy6p7EXpCyQtKSPeyUEoobQgjDSGy5c3VPbShsuNNx1t5H21I38umEwM5Qael7AghXNLhITrhjPZSDnyDfn5azAGmOQe7jDrjH2mCxkEOB3AYruesLYzjrXSAEuwIBe9zimkG7JfJziFNesQZbDpAvsFhwlnPOCYHI04el31YdZuyq9puqYhZqW_BhNW7Mu-2Hz_wOvOjjlGXFKHycT_CNfYpR71yj9ADr8cCj-_eM_Tl9cXn88tm-_7N2_NX28YKJuZGO2qIMa5tBQy-7wYBbhCmk9YR7lormabCuqpp1hvmoa3JUWjF4K2kXvAz9Py4tx75fYEyqxjKGoGeIC1FMd71fV_TX9Fn_6C7tOR6baUEYUww1vJKsSNlcyolg1f7HKLON4oStdaojjWqWqO6rVFdVdPTu9WLieD-WH73VgF-BEqVpq-Q__79n7W_AKwxrV4</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Mohammed, Ibrahim</creator><creator>Ijaz, Sahar</creator><creator>Mokhtari, Tahmineh</creator><creator>Gholaminejhad, Morteza</creator><creator>Mahdavipour, Marzieh</creator><creator>Jameie, Behnamedin</creator><creator>Akbari, Mohammad</creator><creator>Hassanzadeh, Gholamreza</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20200601</creationdate><title>Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation</title><author>Mohammed, Ibrahim ; Ijaz, Sahar ; Mokhtari, Tahmineh ; Gholaminejhad, Morteza ; Mahdavipour, Marzieh ; Jameie, Behnamedin ; Akbari, Mohammad ; Hassanzadeh, Gholamreza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-ad1b0bbd664e9f8794ed94b75cd03d6c52a14cde9fa28b2fe60051e649fc51f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animal models</topic><topic>Apoptosis</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blood-brain barrier</topic><topic>Caspase-1</topic><topic>Cells (biology)</topic><topic>Complex formation</topic><topic>Extracellular vesicles</topic><topic>Gene expression</topic><topic>Inflammasomes</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Injection</topic><topic>Lipids</topic><topic>Membrane vesicles</topic><topic>Metabolic Diseases</topic><topic>Neural stem cells</topic><topic>Neurology</topic><topic>Neurosciences</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Progenitor cells</topic><topic>Proteins</topic><topic>Recovery</topic><topic>Recovery of function</topic><topic>Rodents</topic><topic>Spinal cord injuries</topic><topic>Staining</topic><topic>Subventricular zone</topic><topic>Transplantation</topic><topic>Vesicles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohammed, Ibrahim</creatorcontrib><creatorcontrib>Ijaz, Sahar</creatorcontrib><creatorcontrib>Mokhtari, Tahmineh</creatorcontrib><creatorcontrib>Gholaminejhad, Morteza</creatorcontrib><creatorcontrib>Mahdavipour, Marzieh</creatorcontrib><creatorcontrib>Jameie, Behnamedin</creatorcontrib><creatorcontrib>Akbari, Mohammad</creatorcontrib><creatorcontrib>Hassanzadeh, Gholamreza</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolic brain disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohammed, Ibrahim</au><au>Ijaz, Sahar</au><au>Mokhtari, Tahmineh</au><au>Gholaminejhad, Morteza</au><au>Mahdavipour, Marzieh</au><au>Jameie, Behnamedin</au><au>Akbari, Mohammad</au><au>Hassanzadeh, Gholamreza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation</atitle><jtitle>Metabolic brain disease</jtitle><stitle>Metab Brain Dis</stitle><addtitle>Metab Brain Dis</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>35</volume><issue>5</issue><spage>809</spage><epage>818</epage><pages>809-818</pages><issn>0885-7490</issn><eissn>1573-7365</eissn><abstract>Spinal cord injury (SCI) is the destruction of spinal cord motor and sensory resulted from an attack on the spinal cord, which can cause significant physiological damage. The inflammasome is a multiprotein oligomer resulting in inflammation; the NLRP3 inflammasome composed of NLRP3, apoptosis-associated speck-like protein (ASC), procaspase-1, and cleavage of procaspase-1 into caspase-1 initiates the inflammatory response. Subventricular Zone (SVZ) is the origin of neural stem/progenitor cells (NS/PCs) in the adult brain. Extracellular vesicles (EVs) are tiny lipid membrane bilayer vesicles secreted by different types of cells playing an important role in cell-cell communications. The aim of this study was to investigate the effect of intrathecal transplantation of EVs on the NLRP3 inflammasome formation in SCI rats. Male wistar rats were divided into three groups as following: laminectotomy group, SCI group, and EVs group. EVs was isolated from SVZ, and characterized by western blot and DLS, and then injected into the SCI rats. Real-time PCR and western blot were carried out for gene expression and protein level of NLRP3, ASC, and Caspase-1. H&E and cresyl violet staining were performed for histological analyses, as well as BBB test for motor function. The results indicated high level in mRNA and protein level in SCI group in comparison with laminectomy (
p
< 0.001), and injection of EVs showed a significant reduction in the mRNA and protein levels in EVs group compared to SCI (
p
< 0.001). H&E and cresyl violet staining showed recovery in neural cells of spinal cord tissue in EVs group in comparison with SCI group. BBB test showed the promotion of motor function in EVs group compared to SCI in 14 days (
p
< 0.05). We concluded that the injection of EVs could recover the motor function in rats with SCI and rescue the neural cells of spinal cord tissue by suppressing the formation of the NLRP3 inflammasome complex.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32185593</pmid><doi>10.1007/s11011-020-00563-w</doi><tpages>10</tpages></addata></record> |
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| subjects | Animal models Apoptosis Biochemistry Biomedical and Life Sciences Biomedicine Blood-brain barrier Caspase-1 Cells (biology) Complex formation Extracellular vesicles Gene expression Inflammasomes Inflammation Inflammatory response Injection Lipids Membrane vesicles Metabolic Diseases Neural stem cells Neurology Neurosciences Oncology Original Article Progenitor cells Proteins Recovery Recovery of function Rodents Spinal cord injuries Staining Subventricular zone Transplantation Vesicles |
| title | Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation |
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