Fluid shear stress induces Runx‐2 expression via upregulation of PIEZO1 in MC3T3‐E1 cells
Mechanically induced biological responses in bone cells involve a complex biophysical process. Although various mechanosensors have been identified, the precise mechanotransduction pathway remains poorly understood. PIEZO1 is a newly discovered mechanically activated ion channel in bone cells. This...
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| Veröffentlicht in: | Cell biology international 2020-07, Vol.44 (7), p.1491-1502 |
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| creator | Song, Jidong Liu, Liying Lv, Leifeng Hu, Shugang Tariq, Alkhatatbeh Wang, Wei Dang, Xiaoqian |
| description | Mechanically induced biological responses in bone cells involve a complex biophysical process. Although various mechanosensors have been identified, the precise mechanotransduction pathway remains poorly understood. PIEZO1 is a newly discovered mechanically activated ion channel in bone cells. This study aimed to explore the involvement of PIEZO1 in mechanical loading (fluid shear stress)‐induced signaling cascades that control osteogenesis. The results showed that fluid shear stress increased PIEZO1 expression in MC3T3‐E1 cells. The fluid shear stress elicited the key osteoblastic gene Runx‐2 expression; however, PIEZO1 silencing using small interference RNA blocked these effects. The AKT/GSK‐3β/β‐catenin pathway was activated in this process. PIEZO1 silencing impaired mechanically induced activation of the AKT/GSK‐3β/β‐catenin pathway. Therefore, the results demonstrated that MC3T3‐E1 osteoblasts required PIEZO1 to adapt to the external mechanical fluid shear stress, thereby inducing osteoblastic Runx‐2 gene expression, partly through the AKT/GSK‐3β/β‐catenin pathway. |
| doi_str_mv | 10.1002/cbin.11344 |
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Although various mechanosensors have been identified, the precise mechanotransduction pathway remains poorly understood. PIEZO1 is a newly discovered mechanically activated ion channel in bone cells. This study aimed to explore the involvement of PIEZO1 in mechanical loading (fluid shear stress)‐induced signaling cascades that control osteogenesis. The results showed that fluid shear stress increased PIEZO1 expression in MC3T3‐E1 cells. The fluid shear stress elicited the key osteoblastic gene Runx‐2 expression; however, PIEZO1 silencing using small interference RNA blocked these effects. The AKT/GSK‐3β/β‐catenin pathway was activated in this process. PIEZO1 silencing impaired mechanically induced activation of the AKT/GSK‐3β/β‐catenin pathway. Therefore, the results demonstrated that MC3T3‐E1 osteoblasts required PIEZO1 to adapt to the external mechanical fluid shear stress, thereby inducing osteoblastic Runx‐2 gene expression, partly through the AKT/GSK‐3β/β‐catenin pathway.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1002/cbin.11344</identifier><identifier>PMID: 32181967</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>AKT protein ; Animals ; Catenin ; Core Binding Factor Alpha 1 Subunit - metabolism ; Fluid flow ; fluid shear stress ; Gene expression ; Glycogen Synthase Kinase 3 beta - metabolism ; Ion Channels - metabolism ; MC3T3‐E1 cells ; Mechanical loading ; Mechanical stimuli ; Mechanotransduction ; Mice ; Osteoblasts ; Osteoblasts - metabolism ; Osteocytes - metabolism ; Osteogenesis ; Osteogenesis - physiology ; PIEZO1 ; RNA-mediated interference ; Shear stress ; Signal Transduction - physiology ; siRNA ; Stress, Mechanical ; Transcriptional Activation</subject><ispartof>Cell biology international, 2020-07, Vol.44 (7), p.1491-1502</ispartof><rights>2020 International Federation for Cell Biology</rights><rights>2020 International Federation for Cell Biology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4234-c7366be7d399583d1d9692b6d616472d043f10bd13a7036cb630ade6e6d676ce3</citedby><cites>FETCH-LOGICAL-c4234-c7366be7d399583d1d9692b6d616472d043f10bd13a7036cb630ade6e6d676ce3</cites><orcidid>0000-0002-2858-3274</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbin.11344$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbin.11344$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32181967$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Jidong</creatorcontrib><creatorcontrib>Liu, Liying</creatorcontrib><creatorcontrib>Lv, Leifeng</creatorcontrib><creatorcontrib>Hu, Shugang</creatorcontrib><creatorcontrib>Tariq, Alkhatatbeh</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Dang, Xiaoqian</creatorcontrib><title>Fluid shear stress induces Runx‐2 expression via upregulation of PIEZO1 in MC3T3‐E1 cells</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>Mechanically induced biological responses in bone cells involve a complex biophysical process. Although various mechanosensors have been identified, the precise mechanotransduction pathway remains poorly understood. PIEZO1 is a newly discovered mechanically activated ion channel in bone cells. This study aimed to explore the involvement of PIEZO1 in mechanical loading (fluid shear stress)‐induced signaling cascades that control osteogenesis. The results showed that fluid shear stress increased PIEZO1 expression in MC3T3‐E1 cells. The fluid shear stress elicited the key osteoblastic gene Runx‐2 expression; however, PIEZO1 silencing using small interference RNA blocked these effects. The AKT/GSK‐3β/β‐catenin pathway was activated in this process. PIEZO1 silencing impaired mechanically induced activation of the AKT/GSK‐3β/β‐catenin pathway. Therefore, the results demonstrated that MC3T3‐E1 osteoblasts required PIEZO1 to adapt to the external mechanical fluid shear stress, thereby inducing osteoblastic Runx‐2 gene expression, partly through the AKT/GSK‐3β/β‐catenin pathway.</description><subject>AKT protein</subject><subject>Animals</subject><subject>Catenin</subject><subject>Core Binding Factor Alpha 1 Subunit - metabolism</subject><subject>Fluid flow</subject><subject>fluid shear stress</subject><subject>Gene expression</subject><subject>Glycogen Synthase Kinase 3 beta - metabolism</subject><subject>Ion Channels - metabolism</subject><subject>MC3T3‐E1 cells</subject><subject>Mechanical loading</subject><subject>Mechanical stimuli</subject><subject>Mechanotransduction</subject><subject>Mice</subject><subject>Osteoblasts</subject><subject>Osteoblasts - metabolism</subject><subject>Osteocytes - metabolism</subject><subject>Osteogenesis</subject><subject>Osteogenesis - physiology</subject><subject>PIEZO1</subject><subject>RNA-mediated interference</subject><subject>Shear stress</subject><subject>Signal Transduction - physiology</subject><subject>siRNA</subject><subject>Stress, Mechanical</subject><subject>Transcriptional Activation</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LwzAYxoMobk4vfgAJeBGhM2_Sps1Ry6aD6UTmRZDQNpl2dO1sFt1ufgQ_o5_E1E0PHjy9_37vw8OD0CGQLhBCz7I0L7sAzPe3UBuICLyIBcF20_PA40IELbRnzJQQAD_iu6jFKEQgeNhGj_3C5gqbZ53U2CxqbQzOS2UzbfCdLZef7x8U6-W8OeRViV_zBFs3PdkiWTSLaoJvB72HEbg3fB2zMXMvPcCZLgqzj3YmSWH0waZ20H2_N46vvOHochCfD73Mp8z3spBxnupQMec1YgqU4IKmXHHgfkgV8dkESKqAJSFhPEs5I4nSXDsi5JlmHXSy1p3X1YvVZiFnuWkcJKWurJGUhREhAkTo0OM_6LSydencSeoDFQGBQDjqdE1ldWVMrSdyXuezpF5JILIJXTahy-_QHXy0kbTpTKtf9CdlB8AaeMsLvfpHSsYXg5u16BfVuYtZ</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Song, Jidong</creator><creator>Liu, Liying</creator><creator>Lv, Leifeng</creator><creator>Hu, Shugang</creator><creator>Tariq, Alkhatatbeh</creator><creator>Wang, Wei</creator><creator>Dang, Xiaoqian</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2858-3274</orcidid></search><sort><creationdate>202007</creationdate><title>Fluid shear stress induces Runx‐2 expression via upregulation of PIEZO1 in MC3T3‐E1 cells</title><author>Song, Jidong ; Liu, Liying ; Lv, Leifeng ; Hu, Shugang ; Tariq, Alkhatatbeh ; Wang, Wei ; Dang, Xiaoqian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4234-c7366be7d399583d1d9692b6d616472d043f10bd13a7036cb630ade6e6d676ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>AKT protein</topic><topic>Animals</topic><topic>Catenin</topic><topic>Core Binding Factor Alpha 1 Subunit - metabolism</topic><topic>Fluid flow</topic><topic>fluid shear stress</topic><topic>Gene expression</topic><topic>Glycogen Synthase Kinase 3 beta - metabolism</topic><topic>Ion Channels - metabolism</topic><topic>MC3T3‐E1 cells</topic><topic>Mechanical loading</topic><topic>Mechanical stimuli</topic><topic>Mechanotransduction</topic><topic>Mice</topic><topic>Osteoblasts</topic><topic>Osteoblasts - metabolism</topic><topic>Osteocytes - metabolism</topic><topic>Osteogenesis</topic><topic>Osteogenesis - physiology</topic><topic>PIEZO1</topic><topic>RNA-mediated interference</topic><topic>Shear stress</topic><topic>Signal Transduction - physiology</topic><topic>siRNA</topic><topic>Stress, Mechanical</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Jidong</creatorcontrib><creatorcontrib>Liu, Liying</creatorcontrib><creatorcontrib>Lv, Leifeng</creatorcontrib><creatorcontrib>Hu, Shugang</creatorcontrib><creatorcontrib>Tariq, Alkhatatbeh</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Dang, Xiaoqian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Jidong</au><au>Liu, Liying</au><au>Lv, Leifeng</au><au>Hu, Shugang</au><au>Tariq, Alkhatatbeh</au><au>Wang, Wei</au><au>Dang, Xiaoqian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluid shear stress induces Runx‐2 expression via upregulation of PIEZO1 in MC3T3‐E1 cells</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2020-07</date><risdate>2020</risdate><volume>44</volume><issue>7</issue><spage>1491</spage><epage>1502</epage><pages>1491-1502</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>Mechanically induced biological responses in bone cells involve a complex biophysical process. Although various mechanosensors have been identified, the precise mechanotransduction pathway remains poorly understood. PIEZO1 is a newly discovered mechanically activated ion channel in bone cells. This study aimed to explore the involvement of PIEZO1 in mechanical loading (fluid shear stress)‐induced signaling cascades that control osteogenesis. The results showed that fluid shear stress increased PIEZO1 expression in MC3T3‐E1 cells. The fluid shear stress elicited the key osteoblastic gene Runx‐2 expression; however, PIEZO1 silencing using small interference RNA blocked these effects. The AKT/GSK‐3β/β‐catenin pathway was activated in this process. PIEZO1 silencing impaired mechanically induced activation of the AKT/GSK‐3β/β‐catenin pathway. Therefore, the results demonstrated that MC3T3‐E1 osteoblasts required PIEZO1 to adapt to the external mechanical fluid shear stress, thereby inducing osteoblastic Runx‐2 gene expression, partly through the AKT/GSK‐3β/β‐catenin pathway.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32181967</pmid><doi>10.1002/cbin.11344</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-2858-3274</orcidid></addata></record> |
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| subjects | AKT protein Animals Catenin Core Binding Factor Alpha 1 Subunit - metabolism Fluid flow fluid shear stress Gene expression Glycogen Synthase Kinase 3 beta - metabolism Ion Channels - metabolism MC3T3‐E1 cells Mechanical loading Mechanical stimuli Mechanotransduction Mice Osteoblasts Osteoblasts - metabolism Osteocytes - metabolism Osteogenesis Osteogenesis - physiology PIEZO1 RNA-mediated interference Shear stress Signal Transduction - physiology siRNA Stress, Mechanical Transcriptional Activation |
| title | Fluid shear stress induces Runx‐2 expression via upregulation of PIEZO1 in MC3T3‐E1 cells |
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