Co-Incorporated Mesoporous Carbon Material-Assisted Laser Desorption/Ionization Ion Source as an Online Interface of In Vivo Microdialysis Coupled with Mass Spectrometry
The combination of microdialysis and mass spectrometry (MS) provides the potential for rapidly monitoring diverse metabolites in vivo. Unfortunately, the high concentration of salt in biological microdialysates hindered the sensitive and online detection of these small molecular compounds. In this s...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2020-04, Vol.92 (7), p.5482-5491 |
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| creator | Li, Na Li, Shumu Li, Tuo Yang, Hui Zhang, Yangyang Zhao, Zhenwen |
| description | The combination of microdialysis and mass spectrometry (MS) provides the potential for rapidly monitoring diverse metabolites in vivo. Unfortunately, the high concentration of salt in biological microdialysates hindered the sensitive and online detection of these small molecular compounds. In this study, we synthesized Co-incorporated mesoporous carbon material (Co-NC) and developed a Co-NC-assisted laser desorption/ionization (LDI) ion source as an online interface of in vivo microdialysis coupled with MS for the direct analysis of diverse metabolites in microdialysates. The Co-NC could be used as a matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) analysis of small molecular compounds, even under high concentration salt conditions. The Co-NC possessed the adsorption ability for small molecular compounds, and it was believed that the adsorption ability of Co-NC might separate the analytes from the salt in microdialysates at a microscopic level, which might facilitate the desorption and ionization of the analytes and finally improved the salt-tolerance ability as a matrix. Furthermore, the Co-NC-assisted LDI ion source as a novel interface of in vivo microdialysis coupled with MS has been applied to the online monitoring of liver metabolites from the CCl4-induced liver injury rat model for the first time. |
| doi_str_mv | 10.1021/acs.analchem.0c00227 |
| format | Article |
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Unfortunately, the high concentration of salt in biological microdialysates hindered the sensitive and online detection of these small molecular compounds. In this study, we synthesized Co-incorporated mesoporous carbon material (Co-NC) and developed a Co-NC-assisted laser desorption/ionization (LDI) ion source as an online interface of in vivo microdialysis coupled with MS for the direct analysis of diverse metabolites in microdialysates. The Co-NC could be used as a matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) analysis of small molecular compounds, even under high concentration salt conditions. The Co-NC possessed the adsorption ability for small molecular compounds, and it was believed that the adsorption ability of Co-NC might separate the analytes from the salt in microdialysates at a microscopic level, which might facilitate the desorption and ionization of the analytes and finally improved the salt-tolerance ability as a matrix. Furthermore, the Co-NC-assisted LDI ion source as a novel interface of in vivo microdialysis coupled with MS has been applied to the online monitoring of liver metabolites from the CCl4-induced liver injury rat model for the first time.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.0c00227</identifier><identifier>PMID: 32181652</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Adsorption ; Analytical chemistry ; Animals ; Carbon ; Carbon - chemistry ; Carbon tetrachloride ; Chemical synthesis ; Chemistry ; Coupling (molecular) ; Desorption ; Internet ; Ion sources ; Ionization ; Ions ; Lasers ; Liver ; Liver - metabolism ; Mass spectrometry ; Mass spectroscopy ; Metabolites ; Microdialysis ; Microdialysis - methods ; Monitoring ; Online Systems ; Porosity ; Rats ; Salts ; Salts - chemistry ; Scientific imaging ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Spectroscopy</subject><ispartof>Analytical chemistry (Washington), 2020-04, Vol.92 (7), p.5482-5491</ispartof><rights>Copyright American Chemical Society Apr 7, 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a376t-2752d1d51216649afe9d275c1d3e4a670db78ba5855f432014070d2d598cb5023</citedby><cites>FETCH-LOGICAL-a376t-2752d1d51216649afe9d275c1d3e4a670db78ba5855f432014070d2d598cb5023</cites><orcidid>0000-0001-6127-808X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c00227$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.0c00227$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32181652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Na</creatorcontrib><creatorcontrib>Li, Shumu</creatorcontrib><creatorcontrib>Li, Tuo</creatorcontrib><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>Zhang, Yangyang</creatorcontrib><creatorcontrib>Zhao, Zhenwen</creatorcontrib><title>Co-Incorporated Mesoporous Carbon Material-Assisted Laser Desorption/Ionization Ion Source as an Online Interface of In Vivo Microdialysis Coupled with Mass Spectrometry</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>The combination of microdialysis and mass spectrometry (MS) provides the potential for rapidly monitoring diverse metabolites in vivo. Unfortunately, the high concentration of salt in biological microdialysates hindered the sensitive and online detection of these small molecular compounds. In this study, we synthesized Co-incorporated mesoporous carbon material (Co-NC) and developed a Co-NC-assisted laser desorption/ionization (LDI) ion source as an online interface of in vivo microdialysis coupled with MS for the direct analysis of diverse metabolites in microdialysates. The Co-NC could be used as a matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) analysis of small molecular compounds, even under high concentration salt conditions. The Co-NC possessed the adsorption ability for small molecular compounds, and it was believed that the adsorption ability of Co-NC might separate the analytes from the salt in microdialysates at a microscopic level, which might facilitate the desorption and ionization of the analytes and finally improved the salt-tolerance ability as a matrix. Furthermore, the Co-NC-assisted LDI ion source as a novel interface of in vivo microdialysis coupled with MS has been applied to the online monitoring of liver metabolites from the CCl4-induced liver injury rat model for the first time.</description><subject>Adsorption</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Carbon</subject><subject>Carbon - chemistry</subject><subject>Carbon tetrachloride</subject><subject>Chemical synthesis</subject><subject>Chemistry</subject><subject>Coupling (molecular)</subject><subject>Desorption</subject><subject>Internet</subject><subject>Ion sources</subject><subject>Ionization</subject><subject>Ions</subject><subject>Lasers</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Microdialysis</subject><subject>Microdialysis - methods</subject><subject>Monitoring</subject><subject>Online Systems</subject><subject>Porosity</subject><subject>Rats</subject><subject>Salts</subject><subject>Salts - chemistry</subject><subject>Scientific imaging</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Spectroscopy</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EotvCGyBkiUsv2Y7tOPEeq1DKSrvqocA1chxHdZXYwU6KljfiLZlotz1w4OTxr2_-0cxPyAcGawacXWmT1trr3jzYYQ0GgPPyFVkxySErlOKvyQoARMZLgDNyntIjAGPAirfkTHCmWCH5ivypQrb1JsQxRD3Zlu5tCliHOdFKxyZ4ukc9Ot1n1ym5tDA7nWykn5GM4-SCv9oG737rpaRY0vswR2OpTlR7eud75y3denTpNMqhww_94Z4C3TsTQ4veB3SmVZjHHu1_uekBp6ZE70drphgGO8XDO_Km032y70_vBfn-5eZb9TXb3d1uq-tdpkVZTLiu5C1rJeOsKPKN7uymRc2wVthcFyW0TakaLZWUXS44sBxQ463cKNNI4OKCXB59xxh-zjZN9eCSsX2vvcWr1FyUCi8pSonop3_QR9wcM1kopVSplBBI5UcKd00p2q4eoxt0PNQM6iXKGqOsn6OsT1Fi28eT-dwMtn1pes4OATgCS_vL4P96_gWE-q8z</recordid><startdate>20200407</startdate><enddate>20200407</enddate><creator>Li, Na</creator><creator>Li, Shumu</creator><creator>Li, Tuo</creator><creator>Yang, Hui</creator><creator>Zhang, Yangyang</creator><creator>Zhao, Zhenwen</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6127-808X</orcidid></search><sort><creationdate>20200407</creationdate><title>Co-Incorporated Mesoporous Carbon Material-Assisted Laser Desorption/Ionization Ion Source as an Online Interface of In Vivo Microdialysis Coupled with Mass Spectrometry</title><author>Li, Na ; Li, Shumu ; Li, Tuo ; Yang, Hui ; Zhang, Yangyang ; Zhao, Zhenwen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a376t-2752d1d51216649afe9d275c1d3e4a670db78ba5855f432014070d2d598cb5023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adsorption</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Carbon</topic><topic>Carbon - chemistry</topic><topic>Carbon tetrachloride</topic><topic>Chemical synthesis</topic><topic>Chemistry</topic><topic>Coupling (molecular)</topic><topic>Desorption</topic><topic>Internet</topic><topic>Ion sources</topic><topic>Ionization</topic><topic>Ions</topic><topic>Lasers</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Microdialysis</topic><topic>Microdialysis - methods</topic><topic>Monitoring</topic><topic>Online Systems</topic><topic>Porosity</topic><topic>Rats</topic><topic>Salts</topic><topic>Salts - chemistry</topic><topic>Scientific imaging</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Na</creatorcontrib><creatorcontrib>Li, Shumu</creatorcontrib><creatorcontrib>Li, Tuo</creatorcontrib><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>Zhang, Yangyang</creatorcontrib><creatorcontrib>Zhao, Zhenwen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Na</au><au>Li, Shumu</au><au>Li, Tuo</au><au>Yang, Hui</au><au>Zhang, Yangyang</au><au>Zhao, Zhenwen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Co-Incorporated Mesoporous Carbon Material-Assisted Laser Desorption/Ionization Ion Source as an Online Interface of In Vivo Microdialysis Coupled with Mass Spectrometry</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2020-04-07</date><risdate>2020</risdate><volume>92</volume><issue>7</issue><spage>5482</spage><epage>5491</epage><pages>5482-5491</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>The combination of microdialysis and mass spectrometry (MS) provides the potential for rapidly monitoring diverse metabolites in vivo. Unfortunately, the high concentration of salt in biological microdialysates hindered the sensitive and online detection of these small molecular compounds. In this study, we synthesized Co-incorporated mesoporous carbon material (Co-NC) and developed a Co-NC-assisted laser desorption/ionization (LDI) ion source as an online interface of in vivo microdialysis coupled with MS for the direct analysis of diverse metabolites in microdialysates. The Co-NC could be used as a matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) analysis of small molecular compounds, even under high concentration salt conditions. The Co-NC possessed the adsorption ability for small molecular compounds, and it was believed that the adsorption ability of Co-NC might separate the analytes from the salt in microdialysates at a microscopic level, which might facilitate the desorption and ionization of the analytes and finally improved the salt-tolerance ability as a matrix. Furthermore, the Co-NC-assisted LDI ion source as a novel interface of in vivo microdialysis coupled with MS has been applied to the online monitoring of liver metabolites from the CCl4-induced liver injury rat model for the first time.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>32181652</pmid><doi>10.1021/acs.analchem.0c00227</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-6127-808X</orcidid></addata></record> |
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| subjects | Adsorption Analytical chemistry Animals Carbon Carbon - chemistry Carbon tetrachloride Chemical synthesis Chemistry Coupling (molecular) Desorption Internet Ion sources Ionization Ions Lasers Liver Liver - metabolism Mass spectrometry Mass spectroscopy Metabolites Microdialysis Microdialysis - methods Monitoring Online Systems Porosity Rats Salts Salts - chemistry Scientific imaging Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Spectroscopy |
| title | Co-Incorporated Mesoporous Carbon Material-Assisted Laser Desorption/Ionization Ion Source as an Online Interface of In Vivo Microdialysis Coupled with Mass Spectrometry |
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