Label-free high-throughput photoacoustic tomography of suspected circulating melanoma tumor cells in patients in vivo
Significance: Detection and characterization of circulating tumor cells (CTCs), a key determinant of metastasis, are critical for determining risk of disease progression, understanding metastatic pathways, and facilitating early clinical intervention. Aim: We aim to demonstrate label-free imaging of...
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| Veröffentlicht in: | Journal of biomedical optics 2020-03, Vol.25 (3), p.036002-036002 |
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| creator | Hai, Pengfei Qu, Yuan Li, Yang Zhu, Liren Shmuylovich, Leonid Cornelius, Lynn A Wang, Lihong V |
| description | Significance: Detection and characterization of circulating tumor cells (CTCs), a key determinant of metastasis, are critical for determining risk of disease progression, understanding metastatic pathways, and facilitating early clinical intervention.
Aim: We aim to demonstrate label-free imaging of suspected melanoma CTCs.
Approach: We use a linear-array-based photoacoustic tomography system (LA-PAT) to detect melanoma CTCs, quantify their contrast-to-noise ratios (CNRs), and measure their flow velocities in most of the superficial veins in humans.
Results: With LA-PAT, we successfully imaged suspected melanoma CTCs in patients in vivo, with a CNR >9. CTCs were detected in 3 of 16 patients with stage III or IV melanoma. Among the three CTC-positive patients, two had disease progression; among the 13 CTC-negative patients, 4 showed disease progression.
Conclusions: We suggest that LA-PAT can detect suspected melanoma CTCs in patients in vivo and has potential clinical applications for disease monitoring in melanoma. |
| doi_str_mv | 10.1117/1.JBO.25.3.036002 |
| format | Article |
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Aim: We aim to demonstrate label-free imaging of suspected melanoma CTCs.
Approach: We use a linear-array-based photoacoustic tomography system (LA-PAT) to detect melanoma CTCs, quantify their contrast-to-noise ratios (CNRs), and measure their flow velocities in most of the superficial veins in humans.
Results: With LA-PAT, we successfully imaged suspected melanoma CTCs in patients in vivo, with a CNR >9. CTCs were detected in 3 of 16 patients with stage III or IV melanoma. Among the three CTC-positive patients, two had disease progression; among the 13 CTC-negative patients, 4 showed disease progression.
Conclusions: We suggest that LA-PAT can detect suspected melanoma CTCs in patients in vivo and has potential clinical applications for disease monitoring in melanoma.</description><identifier>ISSN: 1083-3668</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.JBO.25.3.036002</identifier><identifier>PMID: 32170857</identifier><language>eng</language><publisher>United States: Society of Photo-Optical Instrumentation Engineers</publisher><subject>Blood ; Blood vessels ; Flow cytometry ; Flow velocity ; Health risks ; Imaging ; Labels ; Lasers ; Melanoma ; Metastases ; Metastasis ; Noise measurement ; Patient safety ; Photoacoustic effect ; Risk assessment ; Semiconductors ; Skin cancer ; Tissues ; Tomography ; Tumor cells ; Tumors ; Ultrasonic transducers</subject><ispartof>Journal of biomedical optics, 2020-03, Vol.25 (3), p.036002-036002</ispartof><rights>The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.</rights><rights>2020. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 The Authors 2020 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-b815d3bfd16d548f70d85b64d49b7abbf0ed40e001742821a7cedb581efbdf663</citedby><orcidid>0000-0001-7498-133X ; 0000-0001-6670-296X ; 0000-0003-3387-1913 ; 0000-0002-6338-9338 ; 0000-0001-9783-4383</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2860552736/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2860552736?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,21388,27924,27925,33744,33745,43805,53791,53793,64385,64387,64389,72469,74302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32170857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hai, Pengfei</creatorcontrib><creatorcontrib>Qu, Yuan</creatorcontrib><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Zhu, Liren</creatorcontrib><creatorcontrib>Shmuylovich, Leonid</creatorcontrib><creatorcontrib>Cornelius, Lynn A</creatorcontrib><creatorcontrib>Wang, Lihong V</creatorcontrib><title>Label-free high-throughput photoacoustic tomography of suspected circulating melanoma tumor cells in patients in vivo</title><title>Journal of biomedical optics</title><addtitle>J. Biomed. Opt</addtitle><description>Significance: Detection and characterization of circulating tumor cells (CTCs), a key determinant of metastasis, are critical for determining risk of disease progression, understanding metastatic pathways, and facilitating early clinical intervention.
Aim: We aim to demonstrate label-free imaging of suspected melanoma CTCs.
Approach: We use a linear-array-based photoacoustic tomography system (LA-PAT) to detect melanoma CTCs, quantify their contrast-to-noise ratios (CNRs), and measure their flow velocities in most of the superficial veins in humans.
Results: With LA-PAT, we successfully imaged suspected melanoma CTCs in patients in vivo, with a CNR >9. CTCs were detected in 3 of 16 patients with stage III or IV melanoma. Among the three CTC-positive patients, two had disease progression; among the 13 CTC-negative patients, 4 showed disease progression.
Conclusions: We suggest that LA-PAT can detect suspected melanoma CTCs in patients in vivo and has potential clinical applications for disease monitoring in melanoma.</description><subject>Blood</subject><subject>Blood vessels</subject><subject>Flow cytometry</subject><subject>Flow velocity</subject><subject>Health risks</subject><subject>Imaging</subject><subject>Labels</subject><subject>Lasers</subject><subject>Melanoma</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Noise measurement</subject><subject>Patient safety</subject><subject>Photoacoustic effect</subject><subject>Risk assessment</subject><subject>Semiconductors</subject><subject>Skin cancer</subject><subject>Tissues</subject><subject>Tomography</subject><subject>Tumor cells</subject><subject>Tumors</subject><subject>Ultrasonic transducers</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UcmO1TAQjBCIWeADuCBLXLgkeImXd0GCEQygJ80Fzpa3JB4lcfDypPl7_HjDsEicuqWurq7qapoXCHYIIf4GdV_e33SYdqSDhEGIHzXniDLYYizQ49pDQVrCmDhrLlK6hRAKtmNPmzOCEYeC8vOm7JV2cztE58Dkx6nNUwxlnLaSwTaFHJQJJWVvQA5LGKPapjsQBpBK2pzJzgLjoymzyn4dweJmtYZFgVyWEIFx85yAX8FWx27NP_uDP4RnzZNBzck9v6-XzbePH75efWr3N9efr97tW9MzllstELVEDxYxS3sxcGgF1ay3_U5zpfUAne2hgxDxHguMFDfOaiqQG7QdGCOXzdsT71b04qypGqKa5Rb9ouKdDMrLvyern-QYDpJDtsMUV4LX9wQxfC8uZbn4dLSlVlf_IjHhnPSUcVihr_6B3oYS12pPYsEgpZiToyJ0QpkYUopueBCDoDyGKpGsoUpMJZGnUOvOyz9dPGz8SrECuhMgbd79Pvt_xh9TNa9B</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Hai, Pengfei</creator><creator>Qu, Yuan</creator><creator>Li, Yang</creator><creator>Zhu, Liren</creator><creator>Shmuylovich, Leonid</creator><creator>Cornelius, Lynn A</creator><creator>Wang, Lihong V</creator><general>Society of Photo-Optical Instrumentation Engineers</general><general>S P I E - International Society for</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7498-133X</orcidid><orcidid>https://orcid.org/0000-0001-6670-296X</orcidid><orcidid>https://orcid.org/0000-0003-3387-1913</orcidid><orcidid>https://orcid.org/0000-0002-6338-9338</orcidid><orcidid>https://orcid.org/0000-0001-9783-4383</orcidid></search><sort><creationdate>20200301</creationdate><title>Label-free high-throughput photoacoustic tomography of suspected circulating melanoma tumor cells in patients in vivo</title><author>Hai, Pengfei ; 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Biomed. Opt</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>25</volume><issue>3</issue><spage>036002</spage><epage>036002</epage><pages>036002-036002</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><abstract>Significance: Detection and characterization of circulating tumor cells (CTCs), a key determinant of metastasis, are critical for determining risk of disease progression, understanding metastatic pathways, and facilitating early clinical intervention.
Aim: We aim to demonstrate label-free imaging of suspected melanoma CTCs.
Approach: We use a linear-array-based photoacoustic tomography system (LA-PAT) to detect melanoma CTCs, quantify their contrast-to-noise ratios (CNRs), and measure their flow velocities in most of the superficial veins in humans.
Results: With LA-PAT, we successfully imaged suspected melanoma CTCs in patients in vivo, with a CNR >9. CTCs were detected in 3 of 16 patients with stage III or IV melanoma. Among the three CTC-positive patients, two had disease progression; among the 13 CTC-negative patients, 4 showed disease progression.
Conclusions: We suggest that LA-PAT can detect suspected melanoma CTCs in patients in vivo and has potential clinical applications for disease monitoring in melanoma.</abstract><cop>United States</cop><pub>Society of Photo-Optical Instrumentation Engineers</pub><pmid>32170857</pmid><doi>10.1117/1.JBO.25.3.036002</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-7498-133X</orcidid><orcidid>https://orcid.org/0000-0001-6670-296X</orcidid><orcidid>https://orcid.org/0000-0003-3387-1913</orcidid><orcidid>https://orcid.org/0000-0002-6338-9338</orcidid><orcidid>https://orcid.org/0000-0001-9783-4383</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Blood Blood vessels Flow cytometry Flow velocity Health risks Imaging Labels Lasers Melanoma Metastases Metastasis Noise measurement Patient safety Photoacoustic effect Risk assessment Semiconductors Skin cancer Tissues Tomography Tumor cells Tumors Ultrasonic transducers |
| title | Label-free high-throughput photoacoustic tomography of suspected circulating melanoma tumor cells in patients in vivo |
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