Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men
Blunted muscle hypertrophy and impaired regeneration with aging have been partly attributed to satellite cell (SC) dysfunction. However, true muscle regeneration has not yet been studied in elderly individuals. To investigate this, muscle injury was induced by 200 electrically stimulated (ES) eccent...
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| Veröffentlicht in: | The FASEB journal 2020-05, Vol.34 (5), p.6418-6436 |
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| creator | Karlsen, Anders Soendenbroe, Casper Malmgaard‐Clausen, Nikolaj M. Wagener, Frederik Moeller, Casper Emil Senhaji, Zouhir Damberg, Kristine Andersen, Jesper Løvind Schjerling, Peter Kjaer, Michael Mackey, Abigail L. |
| description | Blunted muscle hypertrophy and impaired regeneration with aging have been partly attributed to satellite cell (SC) dysfunction. However, true muscle regeneration has not yet been studied in elderly individuals. To investigate this, muscle injury was induced by 200 electrically stimulated (ES) eccentric contractions of the vastus lateralis (VL) of one leg in seven young (20‐31 years) and 19 elderly men (60‐73 years). This was followed by 13 weeks of resistance training (RT) for both legs to investigate the capacity for hypertrophy. Muscle biopsies were collected Pre‐ and Post‐RT, and 9 days after ES, for immunohistochemistry and RT‐PCR. Hypertrophy was assessed by MRI, DEXA, and immunohistochemistry. Overall, surprisingly comparable responses were observed between the young and elderly. Nine days after ES, Pax7+ SC number had doubled (P |
| doi_str_mv | 10.1096/fj.202000196R |
| format | Article |
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However, true muscle regeneration has not yet been studied in elderly individuals. To investigate this, muscle injury was induced by 200 electrically stimulated (ES) eccentric contractions of the vastus lateralis (VL) of one leg in seven young (20‐31 years) and 19 elderly men (60‐73 years). This was followed by 13 weeks of resistance training (RT) for both legs to investigate the capacity for hypertrophy. Muscle biopsies were collected Pre‐ and Post‐RT, and 9 days after ES, for immunohistochemistry and RT‐PCR. Hypertrophy was assessed by MRI, DEXA, and immunohistochemistry. Overall, surprisingly comparable responses were observed between the young and elderly. Nine days after ES, Pax7+ SC number had doubled (P < .05), alongside necrosis and substantial changes in expression of genes related to matrix, myogenesis, and innervation (P < .05). Post‐RT, VL cross‐sectional area had increased in both legs (~15%, P < .05) and SCs/type II fiber had increased ~2‐4 times more with ES+RT vs RT alone (P < .001). Together these novel findings demonstrate “youthful” regeneration and hypertrophy responses in human elderly muscle. Furthermore, boosting SC availability in healthy elderly men does not enhance the subsequent muscle hypertrophy response to RT.</description><identifier>ISSN: 0892-6638</identifier><identifier>EISSN: 1530-6860</identifier><identifier>DOI: 10.1096/fj.202000196R</identifier><identifier>PMID: 32167202</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Aged ; Aging ; Cell Proliferation ; Female ; heavy resistance training ; human ; Humans ; Hypertrophy - physiopathology ; in vivo myogenesis ; Male ; Middle Aged ; Muscle Development ; Muscle, Skeletal - cytology ; Muscle, Skeletal - physiology ; myofiber necrosis ; myogenic progenitor cells ; Quadriceps Muscle - cytology ; Quadriceps Muscle - physiology ; Regeneration ; Resistance Training ; Sarcopenia ; Satellite Cells, Skeletal Muscle - cytology ; Satellite Cells, Skeletal Muscle - physiology ; Young Adult</subject><ispartof>The FASEB journal, 2020-05, Vol.34 (5), p.6418-6436</ispartof><rights>2020 Federation of American Societies for Experimental Biology</rights><rights>2020 Federation of American Societies for Experimental Biology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4048-e7563773698b6b2a8cade17efba02df3295da7fe9768b7a41e56d91a00ba61093</citedby><cites>FETCH-LOGICAL-c4048-e7563773698b6b2a8cade17efba02df3295da7fe9768b7a41e56d91a00ba61093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1096%2Ffj.202000196R$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1096%2Ffj.202000196R$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32167202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karlsen, Anders</creatorcontrib><creatorcontrib>Soendenbroe, Casper</creatorcontrib><creatorcontrib>Malmgaard‐Clausen, Nikolaj M.</creatorcontrib><creatorcontrib>Wagener, Frederik</creatorcontrib><creatorcontrib>Moeller, Casper Emil</creatorcontrib><creatorcontrib>Senhaji, Zouhir</creatorcontrib><creatorcontrib>Damberg, Kristine</creatorcontrib><creatorcontrib>Andersen, Jesper Løvind</creatorcontrib><creatorcontrib>Schjerling, Peter</creatorcontrib><creatorcontrib>Kjaer, Michael</creatorcontrib><creatorcontrib>Mackey, Abigail L.</creatorcontrib><title>Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men</title><title>The FASEB journal</title><addtitle>FASEB J</addtitle><description>Blunted muscle hypertrophy and impaired regeneration with aging have been partly attributed to satellite cell (SC) dysfunction. However, true muscle regeneration has not yet been studied in elderly individuals. To investigate this, muscle injury was induced by 200 electrically stimulated (ES) eccentric contractions of the vastus lateralis (VL) of one leg in seven young (20‐31 years) and 19 elderly men (60‐73 years). This was followed by 13 weeks of resistance training (RT) for both legs to investigate the capacity for hypertrophy. Muscle biopsies were collected Pre‐ and Post‐RT, and 9 days after ES, for immunohistochemistry and RT‐PCR. Hypertrophy was assessed by MRI, DEXA, and immunohistochemistry. Overall, surprisingly comparable responses were observed between the young and elderly. Nine days after ES, Pax7+ SC number had doubled (P < .05), alongside necrosis and substantial changes in expression of genes related to matrix, myogenesis, and innervation (P < .05). Post‐RT, VL cross‐sectional area had increased in both legs (~15%, P < .05) and SCs/type II fiber had increased ~2‐4 times more with ES+RT vs RT alone (P < .001). Together these novel findings demonstrate “youthful” regeneration and hypertrophy responses in human elderly muscle. Furthermore, boosting SC availability in healthy elderly men does not enhance the subsequent muscle hypertrophy response to RT.</description><subject>Adult</subject><subject>Aged</subject><subject>Aging</subject><subject>Cell Proliferation</subject><subject>Female</subject><subject>heavy resistance training</subject><subject>human</subject><subject>Humans</subject><subject>Hypertrophy - physiopathology</subject><subject>in vivo myogenesis</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Muscle Development</subject><subject>Muscle, Skeletal - cytology</subject><subject>Muscle, Skeletal - physiology</subject><subject>myofiber necrosis</subject><subject>myogenic progenitor cells</subject><subject>Quadriceps Muscle - cytology</subject><subject>Quadriceps Muscle - physiology</subject><subject>Regeneration</subject><subject>Resistance Training</subject><subject>Sarcopenia</subject><subject>Satellite Cells, Skeletal Muscle - cytology</subject><subject>Satellite Cells, Skeletal Muscle - physiology</subject><subject>Young Adult</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtPwzAUhS0EgvIYWZFHBgJ-pHYyAqKAVAnEY45ukmua4jywXVA2fjqpWsrGdHSl7x7pfIQcc3bOWaouzPxcMMEY46l62iIjPpYsUoli22TEklRESslkj-x7P19CjKtdsicFV3p4G5HvR4ce3SeWtIAOiir01LSOeghobRWQFkPSzrW2MuggVG1zRh2-YbO5oCnprO_QBdd2s55WDQ0zpP4dLQawtF74wiJtDZ0h2DAQaEt0tqc1Nodkx4D1eLTOA_I6uXm5voumD7f315fTqIhZnESox0pqLVWa5CoXkBRQItdocmCiNFKk4xK0wVSrJNcQcxyrMuXAWA5qECUPyOmqd5jysUAfsrryy23QYLvwmVi2Sy1iPaDRCi1c671Dk3WuqsH1GWfZUnpm5tmf9IE_WVcv8hrLDf1reQDiFfBVWez_b8smz1dCsFgk8geK647x</recordid><startdate>202005</startdate><enddate>202005</enddate><creator>Karlsen, Anders</creator><creator>Soendenbroe, Casper</creator><creator>Malmgaard‐Clausen, Nikolaj M.</creator><creator>Wagener, Frederik</creator><creator>Moeller, Casper Emil</creator><creator>Senhaji, Zouhir</creator><creator>Damberg, Kristine</creator><creator>Andersen, Jesper Løvind</creator><creator>Schjerling, Peter</creator><creator>Kjaer, Michael</creator><creator>Mackey, Abigail L.</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202005</creationdate><title>Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men</title><author>Karlsen, Anders ; Soendenbroe, Casper ; Malmgaard‐Clausen, Nikolaj M. ; Wagener, Frederik ; Moeller, Casper Emil ; Senhaji, Zouhir ; Damberg, Kristine ; Andersen, Jesper Løvind ; Schjerling, Peter ; Kjaer, Michael ; Mackey, Abigail L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4048-e7563773698b6b2a8cade17efba02df3295da7fe9768b7a41e56d91a00ba61093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aging</topic><topic>Cell Proliferation</topic><topic>Female</topic><topic>heavy resistance training</topic><topic>human</topic><topic>Humans</topic><topic>Hypertrophy - physiopathology</topic><topic>in vivo myogenesis</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Muscle Development</topic><topic>Muscle, Skeletal - cytology</topic><topic>Muscle, Skeletal - physiology</topic><topic>myofiber necrosis</topic><topic>myogenic progenitor cells</topic><topic>Quadriceps Muscle - cytology</topic><topic>Quadriceps Muscle - physiology</topic><topic>Regeneration</topic><topic>Resistance Training</topic><topic>Sarcopenia</topic><topic>Satellite Cells, Skeletal Muscle - cytology</topic><topic>Satellite Cells, Skeletal Muscle - physiology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karlsen, Anders</creatorcontrib><creatorcontrib>Soendenbroe, Casper</creatorcontrib><creatorcontrib>Malmgaard‐Clausen, Nikolaj M.</creatorcontrib><creatorcontrib>Wagener, Frederik</creatorcontrib><creatorcontrib>Moeller, Casper Emil</creatorcontrib><creatorcontrib>Senhaji, Zouhir</creatorcontrib><creatorcontrib>Damberg, Kristine</creatorcontrib><creatorcontrib>Andersen, Jesper Løvind</creatorcontrib><creatorcontrib>Schjerling, Peter</creatorcontrib><creatorcontrib>Kjaer, Michael</creatorcontrib><creatorcontrib>Mackey, Abigail L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The FASEB journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karlsen, Anders</au><au>Soendenbroe, Casper</au><au>Malmgaard‐Clausen, Nikolaj M.</au><au>Wagener, Frederik</au><au>Moeller, Casper Emil</au><au>Senhaji, Zouhir</au><au>Damberg, Kristine</au><au>Andersen, Jesper Løvind</au><au>Schjerling, Peter</au><au>Kjaer, Michael</au><au>Mackey, Abigail L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men</atitle><jtitle>The FASEB journal</jtitle><addtitle>FASEB J</addtitle><date>2020-05</date><risdate>2020</risdate><volume>34</volume><issue>5</issue><spage>6418</spage><epage>6436</epage><pages>6418-6436</pages><issn>0892-6638</issn><eissn>1530-6860</eissn><abstract>Blunted muscle hypertrophy and impaired regeneration with aging have been partly attributed to satellite cell (SC) dysfunction. However, true muscle regeneration has not yet been studied in elderly individuals. To investigate this, muscle injury was induced by 200 electrically stimulated (ES) eccentric contractions of the vastus lateralis (VL) of one leg in seven young (20‐31 years) and 19 elderly men (60‐73 years). This was followed by 13 weeks of resistance training (RT) for both legs to investigate the capacity for hypertrophy. Muscle biopsies were collected Pre‐ and Post‐RT, and 9 days after ES, for immunohistochemistry and RT‐PCR. Hypertrophy was assessed by MRI, DEXA, and immunohistochemistry. Overall, surprisingly comparable responses were observed between the young and elderly. Nine days after ES, Pax7+ SC number had doubled (P < .05), alongside necrosis and substantial changes in expression of genes related to matrix, myogenesis, and innervation (P < .05). Post‐RT, VL cross‐sectional area had increased in both legs (~15%, P < .05) and SCs/type II fiber had increased ~2‐4 times more with ES+RT vs RT alone (P < .001). Together these novel findings demonstrate “youthful” regeneration and hypertrophy responses in human elderly muscle. Furthermore, boosting SC availability in healthy elderly men does not enhance the subsequent muscle hypertrophy response to RT.</abstract><cop>United States</cop><pmid>32167202</pmid><doi>10.1096/fj.202000196R</doi><tpages>19</tpages></addata></record> |
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| subjects | Adult Aged Aging Cell Proliferation Female heavy resistance training human Humans Hypertrophy - physiopathology in vivo myogenesis Male Middle Aged Muscle Development Muscle, Skeletal - cytology Muscle, Skeletal - physiology myofiber necrosis myogenic progenitor cells Quadriceps Muscle - cytology Quadriceps Muscle - physiology Regeneration Resistance Training Sarcopenia Satellite Cells, Skeletal Muscle - cytology Satellite Cells, Skeletal Muscle - physiology Young Adult |
| title | Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men |
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