Aminopeptidase N Activatable Fluorescent Probe for Tracking Metastatic Cancer and Image-Guided Surgery via in Situ Spraying
The recurrence of malignant tumors is mostly caused by incompleted surgical resection. Especially, it is difficult for surgeons to detect and accurately remove metastatic tumors by predominantly using visual examination and palpation owing to the lack of effective means to specifically distinguish t...
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| Veröffentlicht in: | Journal of the American Chemical Society 2020-04, Vol.142 (13), p.6381-6389 |
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| creator | Li, Haidong Yao, Qichao Sun, Wen Shao, Kun Lu, Yang Chung, Jeewon Kim, Dayeh Fan, Jiangli Long, Saran Du, Jianjun Li, Yueqing Wang, Jingyun Yoon, Juyoung Peng, Xiaojun |
| description | The recurrence of malignant tumors is mostly caused by incompleted surgical resection. Especially, it is difficult for surgeons to detect and accurately remove metastatic tumors by predominantly using visual examination and palpation owing to the lack of effective means to specifically distinguish the boundary range between normal and tumor tissues. Thus, the development of activated fluorescent probe with superior tumor-to-normal (T/N) tissue ratios is particularly urgent in clinics. In view of CD13/aminopeptidase N (APN) regarded as a cancer-specific biomarker, mediating with progression, invasion, and migration of malignant tumor, herein, we reported an APN-responsive fluorescent probe YH-APN and demonstrated its application to distinguish cancer cells. Through in situ spraying manner, fluorescent superior tumor-to-normal (T/N) tissue ratios (subcutaneous transplantation tumor, 13.86; hepatic metastasis, 4.42 and 6.25; splenic metastasis, 4.99) were achieved. More importantly, we have demonstrated the ability to image metastasis tumor tissue less than 1 mm in diameter, highlighting the potential for this probe to be used as a tool in surgical resection. This research may spur the use of enzyme-activatable fluorescent probes for the progress of tumor diagnosis and image-guided surgery (IGS). |
| doi_str_mv | 10.1021/jacs.0c01365 |
| format | Article |
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Especially, it is difficult for surgeons to detect and accurately remove metastatic tumors by predominantly using visual examination and palpation owing to the lack of effective means to specifically distinguish the boundary range between normal and tumor tissues. Thus, the development of activated fluorescent probe with superior tumor-to-normal (T/N) tissue ratios is particularly urgent in clinics. In view of CD13/aminopeptidase N (APN) regarded as a cancer-specific biomarker, mediating with progression, invasion, and migration of malignant tumor, herein, we reported an APN-responsive fluorescent probe YH-APN and demonstrated its application to distinguish cancer cells. Through in situ spraying manner, fluorescent superior tumor-to-normal (T/N) tissue ratios (subcutaneous transplantation tumor, 13.86; hepatic metastasis, 4.42 and 6.25; splenic metastasis, 4.99) were achieved. More importantly, we have demonstrated the ability to image metastasis tumor tissue less than 1 mm in diameter, highlighting the potential for this probe to be used as a tool in surgical resection. This research may spur the use of enzyme-activatable fluorescent probes for the progress of tumor diagnosis and image-guided surgery (IGS).</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/jacs.0c01365</identifier><identifier>PMID: 32167306</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; CD13 Antigens - analysis ; Female ; Fluorescent Dyes - chemistry ; Hep G2 Cells ; Humans ; Mice ; Mice, Inbred BALB C ; Models, Molecular ; Neoplasm Metastasis - diagnostic imaging ; Neoplasms - diagnostic imaging ; Neoplasms - surgery ; Optical Imaging - methods ; Surgery, Computer-Assisted</subject><ispartof>Journal of the American Chemical Society, 2020-04, Vol.142 (13), p.6381-6389</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a390t-993f12a8516c7e76a8bd3d5afb7571841c51551788d5b52fd341ebe423f55c9e3</citedby><cites>FETCH-LOGICAL-a390t-993f12a8516c7e76a8bd3d5afb7571841c51551788d5b52fd341ebe423f55c9e3</cites><orcidid>0000-0003-3248-9747 ; 0000-0002-1728-3970 ; 0000-0001-7777-079X ; 0000-0003-4962-5186 ; 0000-0002-2721-0337 ; 0000-0002-8806-322X ; 0000-0003-4316-5350</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jacs.0c01365$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jacs.0c01365$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32167306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Haidong</creatorcontrib><creatorcontrib>Yao, Qichao</creatorcontrib><creatorcontrib>Sun, Wen</creatorcontrib><creatorcontrib>Shao, Kun</creatorcontrib><creatorcontrib>Lu, Yang</creatorcontrib><creatorcontrib>Chung, Jeewon</creatorcontrib><creatorcontrib>Kim, Dayeh</creatorcontrib><creatorcontrib>Fan, Jiangli</creatorcontrib><creatorcontrib>Long, Saran</creatorcontrib><creatorcontrib>Du, Jianjun</creatorcontrib><creatorcontrib>Li, Yueqing</creatorcontrib><creatorcontrib>Wang, Jingyun</creatorcontrib><creatorcontrib>Yoon, Juyoung</creatorcontrib><creatorcontrib>Peng, Xiaojun</creatorcontrib><title>Aminopeptidase N Activatable Fluorescent Probe for Tracking Metastatic Cancer and Image-Guided Surgery via in Situ Spraying</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>The recurrence of malignant tumors is mostly caused by incompleted surgical resection. Especially, it is difficult for surgeons to detect and accurately remove metastatic tumors by predominantly using visual examination and palpation owing to the lack of effective means to specifically distinguish the boundary range between normal and tumor tissues. Thus, the development of activated fluorescent probe with superior tumor-to-normal (T/N) tissue ratios is particularly urgent in clinics. In view of CD13/aminopeptidase N (APN) regarded as a cancer-specific biomarker, mediating with progression, invasion, and migration of malignant tumor, herein, we reported an APN-responsive fluorescent probe YH-APN and demonstrated its application to distinguish cancer cells. Through in situ spraying manner, fluorescent superior tumor-to-normal (T/N) tissue ratios (subcutaneous transplantation tumor, 13.86; hepatic metastasis, 4.42 and 6.25; splenic metastasis, 4.99) were achieved. More importantly, we have demonstrated the ability to image metastasis tumor tissue less than 1 mm in diameter, highlighting the potential for this probe to be used as a tool in surgical resection. 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Yao, Qichao ; Sun, Wen ; Shao, Kun ; Lu, Yang ; Chung, Jeewon ; Kim, Dayeh ; Fan, Jiangli ; Long, Saran ; Du, Jianjun ; Li, Yueqing ; Wang, Jingyun ; Yoon, Juyoung ; Peng, Xiaojun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a390t-993f12a8516c7e76a8bd3d5afb7571841c51551788d5b52fd341ebe423f55c9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>CD13 Antigens - analysis</topic><topic>Female</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Models, Molecular</topic><topic>Neoplasm Metastasis - diagnostic imaging</topic><topic>Neoplasms - diagnostic imaging</topic><topic>Neoplasms - surgery</topic><topic>Optical Imaging - methods</topic><topic>Surgery, Computer-Assisted</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Haidong</creatorcontrib><creatorcontrib>Yao, Qichao</creatorcontrib><creatorcontrib>Sun, Wen</creatorcontrib><creatorcontrib>Shao, Kun</creatorcontrib><creatorcontrib>Lu, Yang</creatorcontrib><creatorcontrib>Chung, Jeewon</creatorcontrib><creatorcontrib>Kim, Dayeh</creatorcontrib><creatorcontrib>Fan, Jiangli</creatorcontrib><creatorcontrib>Long, Saran</creatorcontrib><creatorcontrib>Du, Jianjun</creatorcontrib><creatorcontrib>Li, Yueqing</creatorcontrib><creatorcontrib>Wang, Jingyun</creatorcontrib><creatorcontrib>Yoon, Juyoung</creatorcontrib><creatorcontrib>Peng, Xiaojun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Haidong</au><au>Yao, Qichao</au><au>Sun, Wen</au><au>Shao, Kun</au><au>Lu, Yang</au><au>Chung, Jeewon</au><au>Kim, Dayeh</au><au>Fan, Jiangli</au><au>Long, Saran</au><au>Du, Jianjun</au><au>Li, Yueqing</au><au>Wang, Jingyun</au><au>Yoon, Juyoung</au><au>Peng, Xiaojun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aminopeptidase N Activatable Fluorescent Probe for Tracking Metastatic Cancer and Image-Guided Surgery via in Situ Spraying</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2020-04-01</date><risdate>2020</risdate><volume>142</volume><issue>13</issue><spage>6381</spage><epage>6389</epage><pages>6381-6389</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><abstract>The recurrence of malignant tumors is mostly caused by incompleted surgical resection. Especially, it is difficult for surgeons to detect and accurately remove metastatic tumors by predominantly using visual examination and palpation owing to the lack of effective means to specifically distinguish the boundary range between normal and tumor tissues. Thus, the development of activated fluorescent probe with superior tumor-to-normal (T/N) tissue ratios is particularly urgent in clinics. In view of CD13/aminopeptidase N (APN) regarded as a cancer-specific biomarker, mediating with progression, invasion, and migration of malignant tumor, herein, we reported an APN-responsive fluorescent probe YH-APN and demonstrated its application to distinguish cancer cells. Through in situ spraying manner, fluorescent superior tumor-to-normal (T/N) tissue ratios (subcutaneous transplantation tumor, 13.86; hepatic metastasis, 4.42 and 6.25; splenic metastasis, 4.99) were achieved. More importantly, we have demonstrated the ability to image metastasis tumor tissue less than 1 mm in diameter, highlighting the potential for this probe to be used as a tool in surgical resection. 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| subjects | Animals CD13 Antigens - analysis Female Fluorescent Dyes - chemistry Hep G2 Cells Humans Mice Mice, Inbred BALB C Models, Molecular Neoplasm Metastasis - diagnostic imaging Neoplasms - diagnostic imaging Neoplasms - surgery Optical Imaging - methods Surgery, Computer-Assisted |
| title | Aminopeptidase N Activatable Fluorescent Probe for Tracking Metastatic Cancer and Image-Guided Surgery via in Situ Spraying |
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