Simulated Moving Bed Purification for Flavonoids from Tartary Buckwheat Shell
Abstract Tartary buckwheat shell is an important by-product of Tartary buckwheat production. Previous studies shown that Tartary buckwheat shells are rich in flavonoids, which are responsible for their antioxidant properties. Due to lack of advanced separation technologies, the purification for Tart...
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| Veröffentlicht in: | Journal of chromatographic science 2020-04, Vol.58 (4), p.362-372 |
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| creator | Li, Liangyu Liu, Wanxia Song, Dawei Li, Chaoyang Jia, Pengyu Niu, Guagcai |
| description | Abstract
Tartary buckwheat shell is an important by-product of Tartary buckwheat production. Previous studies shown that Tartary buckwheat shells are rich in flavonoids, which are responsible for their antioxidant properties. Due to lack of advanced separation technologies, the purification for Tartary buckwheat shell is still in the laboratory scale, and could not realize the industrialization production. According to the results of static adsorption experiment, AB-8 resin was selected for Tartary buckwheat shell flavonoids (TBSF) adsorption. The adsorption isotherm, resin adsorption thermodynamic and dynamic adsorption parameters were studied. And the adsorption of AB-8 resin for TBSF was determined as an endothermic process. Results of preparative chromatography experiment showed that TBSF could be efficiently purified by AB-8 resin. And the optimal parameters were: feed concentration 25 mg/mL, desorption flow rate 2.5 mL/min. Under these conditions, the TBSF were separated effectively. Results of liquid chromatography-mass spectrometer (LC-MS) indicated that there were seven kinds of flavonoids in Tartary buckwheat shell, which were mainly from the 40 and 60% of ethanol elution. Simulated moving bed (SMB) was applied for TBSF purification the first time in this study. The optimal conditions of SMB were as following: adsorption zone flow rate 7.0 mL/min, contaminant removal zone flow rate 17.9 mL/min, product elution zone flow rate 22.3 mL/min, regeneration zone flow rate 21.5 mL/min, water washing zone flow rate 27.5 mL/min, switching time 1260 S, and the purity and yield of TBSF was 90 ± 0.22% and 85 ± 0.28%, respectively. The IC50 values of α-glucosidase inhibition activities and DPPH scavenging activity of the purified TBSF were 57.09 ± 0.15 and 7.92 ± 0.23 μg/mL, respectively. The constituents of TBSF showed higher α-glucosidase inhibition activities and antioxidant than raw TBSF and rutin. The results suggest that SMB is a proper method for industrial production of TBSF, and SMB could be applied for other natural products purification. |
| doi_str_mv | 10.1093/chromsci/bmz122 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2376733965</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/chromsci/bmz122</oup_id><sourcerecordid>2376733965</sourcerecordid><originalsourceid>FETCH-LOGICAL-c332t-90377d18ac6fb10aa5fc687cf08cbccfbb29314b5aac638c8a654a4187fa27af3</originalsourceid><addsrcrecordid>eNqFkM9LwzAUx4Mobk7P3iRHEeryo03aoxtOhYnCJngraZq4aNvMpJ3oX29GN6-e3nvwed_3-ABwjtE1Rhkdy5WztZdmXNQ_mJADMMRZnESEZq-HYIgQwVHGWDIAJ96_b0ecJsdgQAlmFBM-BI8LU3eVaFUJH-3GNG9wEtrnzhltpGiNbaC2Ds4qsbGNNaWHOlyES-Fa4b7hpJMfXyslWrhYqao6BUdaVF6d7eoIvMxul9P7aP509zC9mUeSUtJGGaKclzgVkukCIyESLVnKpUapLKTURUEyiuMiEYGgqUwFS2IR45RrQbjQdAQu-9y1s5-d8m1eGy_DA6JRtvM5oZxxSjOWBHTco9JZ753S-dqZOvyeY5RvHeZ7h3nvMGxc7MK7olblH7-XFoCrHrDd-t-0X3YUf8U</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2376733965</pqid></control><display><type>article</type><title>Simulated Moving Bed Purification for Flavonoids from Tartary Buckwheat Shell</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Li, Liangyu ; Liu, Wanxia ; Song, Dawei ; Li, Chaoyang ; Jia, Pengyu ; Niu, Guagcai</creator><creatorcontrib>Li, Liangyu ; Liu, Wanxia ; Song, Dawei ; Li, Chaoyang ; Jia, Pengyu ; Niu, Guagcai</creatorcontrib><description>Abstract
Tartary buckwheat shell is an important by-product of Tartary buckwheat production. Previous studies shown that Tartary buckwheat shells are rich in flavonoids, which are responsible for their antioxidant properties. Due to lack of advanced separation technologies, the purification for Tartary buckwheat shell is still in the laboratory scale, and could not realize the industrialization production. According to the results of static adsorption experiment, AB-8 resin was selected for Tartary buckwheat shell flavonoids (TBSF) adsorption. The adsorption isotherm, resin adsorption thermodynamic and dynamic adsorption parameters were studied. And the adsorption of AB-8 resin for TBSF was determined as an endothermic process. Results of preparative chromatography experiment showed that TBSF could be efficiently purified by AB-8 resin. And the optimal parameters were: feed concentration 25 mg/mL, desorption flow rate 2.5 mL/min. Under these conditions, the TBSF were separated effectively. Results of liquid chromatography-mass spectrometer (LC-MS) indicated that there were seven kinds of flavonoids in Tartary buckwheat shell, which were mainly from the 40 and 60% of ethanol elution. Simulated moving bed (SMB) was applied for TBSF purification the first time in this study. The optimal conditions of SMB were as following: adsorption zone flow rate 7.0 mL/min, contaminant removal zone flow rate 17.9 mL/min, product elution zone flow rate 22.3 mL/min, regeneration zone flow rate 21.5 mL/min, water washing zone flow rate 27.5 mL/min, switching time 1260 S, and the purity and yield of TBSF was 90 ± 0.22% and 85 ± 0.28%, respectively. The IC50 values of α-glucosidase inhibition activities and DPPH scavenging activity of the purified TBSF were 57.09 ± 0.15 and 7.92 ± 0.23 μg/mL, respectively. The constituents of TBSF showed higher α-glucosidase inhibition activities and antioxidant than raw TBSF and rutin. The results suggest that SMB is a proper method for industrial production of TBSF, and SMB could be applied for other natural products purification.</description><identifier>ISSN: 0021-9665</identifier><identifier>EISSN: 1945-239X</identifier><identifier>DOI: 10.1093/chromsci/bmz122</identifier><identifier>PMID: 32163127</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Chemical Fractionation ; Chromatography, Liquid - instrumentation ; Chromatography, Liquid - methods ; Fagopyrum - anatomy & histology ; Fagopyrum - chemistry ; Flavonoids - isolation & purification ; Flavonoids - pharmacology ; Food-Processing Industry ; Free Radical Scavengers - chemistry ; Free Radical Scavengers - pharmacology ; Glycoside Hydrolase Inhibitors - chemistry ; Glycoside Hydrolase Inhibitors - pharmacology ; Kinetics ; Mass Spectrometry ; Thermodynamics</subject><ispartof>Journal of chromatographic science, 2020-04, Vol.58 (4), p.362-372</ispartof><rights>The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com 2020</rights><rights>The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-90377d18ac6fb10aa5fc687cf08cbccfbb29314b5aac638c8a654a4187fa27af3</citedby><cites>FETCH-LOGICAL-c332t-90377d18ac6fb10aa5fc687cf08cbccfbb29314b5aac638c8a654a4187fa27af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32163127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Liangyu</creatorcontrib><creatorcontrib>Liu, Wanxia</creatorcontrib><creatorcontrib>Song, Dawei</creatorcontrib><creatorcontrib>Li, Chaoyang</creatorcontrib><creatorcontrib>Jia, Pengyu</creatorcontrib><creatorcontrib>Niu, Guagcai</creatorcontrib><title>Simulated Moving Bed Purification for Flavonoids from Tartary Buckwheat Shell</title><title>Journal of chromatographic science</title><addtitle>J Chromatogr Sci</addtitle><description>Abstract
Tartary buckwheat shell is an important by-product of Tartary buckwheat production. Previous studies shown that Tartary buckwheat shells are rich in flavonoids, which are responsible for their antioxidant properties. Due to lack of advanced separation technologies, the purification for Tartary buckwheat shell is still in the laboratory scale, and could not realize the industrialization production. According to the results of static adsorption experiment, AB-8 resin was selected for Tartary buckwheat shell flavonoids (TBSF) adsorption. The adsorption isotherm, resin adsorption thermodynamic and dynamic adsorption parameters were studied. And the adsorption of AB-8 resin for TBSF was determined as an endothermic process. Results of preparative chromatography experiment showed that TBSF could be efficiently purified by AB-8 resin. And the optimal parameters were: feed concentration 25 mg/mL, desorption flow rate 2.5 mL/min. Under these conditions, the TBSF were separated effectively. Results of liquid chromatography-mass spectrometer (LC-MS) indicated that there were seven kinds of flavonoids in Tartary buckwheat shell, which were mainly from the 40 and 60% of ethanol elution. Simulated moving bed (SMB) was applied for TBSF purification the first time in this study. The optimal conditions of SMB were as following: adsorption zone flow rate 7.0 mL/min, contaminant removal zone flow rate 17.9 mL/min, product elution zone flow rate 22.3 mL/min, regeneration zone flow rate 21.5 mL/min, water washing zone flow rate 27.5 mL/min, switching time 1260 S, and the purity and yield of TBSF was 90 ± 0.22% and 85 ± 0.28%, respectively. The IC50 values of α-glucosidase inhibition activities and DPPH scavenging activity of the purified TBSF were 57.09 ± 0.15 and 7.92 ± 0.23 μg/mL, respectively. The constituents of TBSF showed higher α-glucosidase inhibition activities and antioxidant than raw TBSF and rutin. The results suggest that SMB is a proper method for industrial production of TBSF, and SMB could be applied for other natural products purification.</description><subject>Chemical Fractionation</subject><subject>Chromatography, Liquid - instrumentation</subject><subject>Chromatography, Liquid - methods</subject><subject>Fagopyrum - anatomy & histology</subject><subject>Fagopyrum - chemistry</subject><subject>Flavonoids - isolation & purification</subject><subject>Flavonoids - pharmacology</subject><subject>Food-Processing Industry</subject><subject>Free Radical Scavengers - chemistry</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Glycoside Hydrolase Inhibitors - chemistry</subject><subject>Glycoside Hydrolase Inhibitors - pharmacology</subject><subject>Kinetics</subject><subject>Mass Spectrometry</subject><subject>Thermodynamics</subject><issn>0021-9665</issn><issn>1945-239X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9LwzAUx4Mobk7P3iRHEeryo03aoxtOhYnCJngraZq4aNvMpJ3oX29GN6-e3nvwed_3-ABwjtE1Rhkdy5WztZdmXNQ_mJADMMRZnESEZq-HYIgQwVHGWDIAJ96_b0ecJsdgQAlmFBM-BI8LU3eVaFUJH-3GNG9wEtrnzhltpGiNbaC2Ds4qsbGNNaWHOlyES-Fa4b7hpJMfXyslWrhYqao6BUdaVF6d7eoIvMxul9P7aP509zC9mUeSUtJGGaKclzgVkukCIyESLVnKpUapLKTURUEyiuMiEYGgqUwFS2IR45RrQbjQdAQu-9y1s5-d8m1eGy_DA6JRtvM5oZxxSjOWBHTco9JZ753S-dqZOvyeY5RvHeZ7h3nvMGxc7MK7olblH7-XFoCrHrDd-t-0X3YUf8U</recordid><startdate>20200423</startdate><enddate>20200423</enddate><creator>Li, Liangyu</creator><creator>Liu, Wanxia</creator><creator>Song, Dawei</creator><creator>Li, Chaoyang</creator><creator>Jia, Pengyu</creator><creator>Niu, Guagcai</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200423</creationdate><title>Simulated Moving Bed Purification for Flavonoids from Tartary Buckwheat Shell</title><author>Li, Liangyu ; Liu, Wanxia ; Song, Dawei ; Li, Chaoyang ; Jia, Pengyu ; Niu, Guagcai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-90377d18ac6fb10aa5fc687cf08cbccfbb29314b5aac638c8a654a4187fa27af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Chemical Fractionation</topic><topic>Chromatography, Liquid - instrumentation</topic><topic>Chromatography, Liquid - methods</topic><topic>Fagopyrum - anatomy & histology</topic><topic>Fagopyrum - chemistry</topic><topic>Flavonoids - isolation & purification</topic><topic>Flavonoids - pharmacology</topic><topic>Food-Processing Industry</topic><topic>Free Radical Scavengers - chemistry</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Glycoside Hydrolase Inhibitors - chemistry</topic><topic>Glycoside Hydrolase Inhibitors - pharmacology</topic><topic>Kinetics</topic><topic>Mass Spectrometry</topic><topic>Thermodynamics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Liangyu</creatorcontrib><creatorcontrib>Liu, Wanxia</creatorcontrib><creatorcontrib>Song, Dawei</creatorcontrib><creatorcontrib>Li, Chaoyang</creatorcontrib><creatorcontrib>Jia, Pengyu</creatorcontrib><creatorcontrib>Niu, Guagcai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatographic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Liangyu</au><au>Liu, Wanxia</au><au>Song, Dawei</au><au>Li, Chaoyang</au><au>Jia, Pengyu</au><au>Niu, Guagcai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simulated Moving Bed Purification for Flavonoids from Tartary Buckwheat Shell</atitle><jtitle>Journal of chromatographic science</jtitle><addtitle>J Chromatogr Sci</addtitle><date>2020-04-23</date><risdate>2020</risdate><volume>58</volume><issue>4</issue><spage>362</spage><epage>372</epage><pages>362-372</pages><issn>0021-9665</issn><eissn>1945-239X</eissn><abstract>Abstract
Tartary buckwheat shell is an important by-product of Tartary buckwheat production. Previous studies shown that Tartary buckwheat shells are rich in flavonoids, which are responsible for their antioxidant properties. Due to lack of advanced separation technologies, the purification for Tartary buckwheat shell is still in the laboratory scale, and could not realize the industrialization production. According to the results of static adsorption experiment, AB-8 resin was selected for Tartary buckwheat shell flavonoids (TBSF) adsorption. The adsorption isotherm, resin adsorption thermodynamic and dynamic adsorption parameters were studied. And the adsorption of AB-8 resin for TBSF was determined as an endothermic process. Results of preparative chromatography experiment showed that TBSF could be efficiently purified by AB-8 resin. And the optimal parameters were: feed concentration 25 mg/mL, desorption flow rate 2.5 mL/min. Under these conditions, the TBSF were separated effectively. Results of liquid chromatography-mass spectrometer (LC-MS) indicated that there were seven kinds of flavonoids in Tartary buckwheat shell, which were mainly from the 40 and 60% of ethanol elution. Simulated moving bed (SMB) was applied for TBSF purification the first time in this study. The optimal conditions of SMB were as following: adsorption zone flow rate 7.0 mL/min, contaminant removal zone flow rate 17.9 mL/min, product elution zone flow rate 22.3 mL/min, regeneration zone flow rate 21.5 mL/min, water washing zone flow rate 27.5 mL/min, switching time 1260 S, and the purity and yield of TBSF was 90 ± 0.22% and 85 ± 0.28%, respectively. The IC50 values of α-glucosidase inhibition activities and DPPH scavenging activity of the purified TBSF were 57.09 ± 0.15 and 7.92 ± 0.23 μg/mL, respectively. The constituents of TBSF showed higher α-glucosidase inhibition activities and antioxidant than raw TBSF and rutin. The results suggest that SMB is a proper method for industrial production of TBSF, and SMB could be applied for other natural products purification.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>32163127</pmid><doi>10.1093/chromsci/bmz122</doi><tpages>11</tpages></addata></record> |
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| subjects | Chemical Fractionation Chromatography, Liquid - instrumentation Chromatography, Liquid - methods Fagopyrum - anatomy & histology Fagopyrum - chemistry Flavonoids - isolation & purification Flavonoids - pharmacology Food-Processing Industry Free Radical Scavengers - chemistry Free Radical Scavengers - pharmacology Glycoside Hydrolase Inhibitors - chemistry Glycoside Hydrolase Inhibitors - pharmacology Kinetics Mass Spectrometry Thermodynamics |
| title | Simulated Moving Bed Purification for Flavonoids from Tartary Buckwheat Shell |
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