CircMACF1 Attenuates Acute Myocardial Infarction Through miR-500b-5p-EMP1 Axis
It is widely accepted that circular RNA (circRNA) plays an important role in cardiovascular diseases. Therefore, this experiment aimed to investigate the pathogenesis of circMACF1 in acute myocardial infarction (AMI). qRT-PCR and immunoblotting were used to detect the expression levels of circMACF1,...
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| Veröffentlicht in: | Journal of cardiovascular translational research 2021-02, Vol.14 (1), p.161-172 |
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| creator | Zhao, Bo Li, Guangping Peng, Jianjun Ren, Lihui Lei, Licheng Ye, Huiming Wang, Zuoyan Zhao, Sheng |
| description | It is widely accepted that circular RNA (circRNA) plays an important role in cardiovascular diseases. Therefore, this experiment aimed to investigate the pathogenesis of circMACF1 in acute myocardial infarction (AMI). qRT-PCR and immunoblotting were used to detect the expression levels of circMACF1, miR-500b-5p, and epithelial membrane protein 1 (EMP1). The role of circMACF1, miR-500b-5p, and EMP1 in cardiomyocyte apoptosis was assessed using annexin V-FITC/PI. Echocardiographic assessment, serum creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH), myocardial infarct size, and TUNEL staining were applied in our research. In the MI group, the expression levels of circMACF1 and EMP1 were decreased with the increasing expression level of miR-500b-5p. CircMACF1 upregulated the expression of EMP1 as a sponge of miR-500b-5p, and circMACF1 was a direct target of miR-500b-5p. CircMACF1 impaired the progression of AMI by modulating the miR-500b-5p/EMP1 axis. CircMACF1 may be a potential therapeutic target for treating AMI.
Graphical Abstract
CircMACF1 upregulated EMP1 expression by sponge miR-500b-5p. |
| doi_str_mv | 10.1007/s12265-020-09976-5 |
| format | Article |
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Graphical Abstract
CircMACF1 upregulated EMP1 expression by sponge miR-500b-5p.</description><identifier>ISSN: 1937-5387</identifier><identifier>EISSN: 1937-5395</identifier><identifier>DOI: 10.1007/s12265-020-09976-5</identifier><identifier>PMID: 32162171</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biomedical Engineering and Bioengineering ; Biomedicine ; Cardiology ; Human Genetics ; Medicine ; Medicine & Public Health ; Original Article</subject><ispartof>Journal of cardiovascular translational research, 2021-02, Vol.14 (1), p.161-172</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-e42cd76aa14e5a5bfa21528488bb61b6d249d4c88ea061f551464720547f9983</citedby><cites>FETCH-LOGICAL-c413t-e42cd76aa14e5a5bfa21528488bb61b6d249d4c88ea061f551464720547f9983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12265-020-09976-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12265-020-09976-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32162171$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Bo</creatorcontrib><creatorcontrib>Li, Guangping</creatorcontrib><creatorcontrib>Peng, Jianjun</creatorcontrib><creatorcontrib>Ren, Lihui</creatorcontrib><creatorcontrib>Lei, Licheng</creatorcontrib><creatorcontrib>Ye, Huiming</creatorcontrib><creatorcontrib>Wang, Zuoyan</creatorcontrib><creatorcontrib>Zhao, Sheng</creatorcontrib><title>CircMACF1 Attenuates Acute Myocardial Infarction Through miR-500b-5p-EMP1 Axis</title><title>Journal of cardiovascular translational research</title><addtitle>J. of Cardiovasc. Trans. Res</addtitle><addtitle>J Cardiovasc Transl Res</addtitle><description>It is widely accepted that circular RNA (circRNA) plays an important role in cardiovascular diseases. Therefore, this experiment aimed to investigate the pathogenesis of circMACF1 in acute myocardial infarction (AMI). qRT-PCR and immunoblotting were used to detect the expression levels of circMACF1, miR-500b-5p, and epithelial membrane protein 1 (EMP1). The role of circMACF1, miR-500b-5p, and EMP1 in cardiomyocyte apoptosis was assessed using annexin V-FITC/PI. Echocardiographic assessment, serum creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH), myocardial infarct size, and TUNEL staining were applied in our research. In the MI group, the expression levels of circMACF1 and EMP1 were decreased with the increasing expression level of miR-500b-5p. CircMACF1 upregulated the expression of EMP1 as a sponge of miR-500b-5p, and circMACF1 was a direct target of miR-500b-5p. CircMACF1 impaired the progression of AMI by modulating the miR-500b-5p/EMP1 axis. CircMACF1 may be a potential therapeutic target for treating AMI.
Graphical Abstract
CircMACF1 upregulated EMP1 expression by sponge miR-500b-5p.</description><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedicine</subject><subject>Cardiology</subject><subject>Human Genetics</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original Article</subject><issn>1937-5387</issn><issn>1937-5395</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRS0EolD4ARYoSzYGv-0so6iFSi0g1L3lOE6bKo9iJxL9ewIpXbKakebcK80B4A6jR4yQfAqYEMEhIgiiOJYC8jNwhWMqIacxPz_tSk7AdQg7hARBUl6CCSVYECzxFXhNS29XSTrHUdJ1rulN50KU2L5z0erQWuPz0lTRoimMt13ZNtF669t-s43q8gNyhDLI93C2eh_yX2W4AReFqYK7Pc4pWM9n6_QFLt-eF2myhJZh2kHHiM2lMAYzxw3PCkMwJ4oplWUCZyInLM6ZVcoZJHDBOWaCSYI4k0UcKzoFD2Pt3refvQudrstgXVWZxrV90IRKISlRCA0oGVHr2xC8K_Tel7XxB42R_tGoR4160Kh_NWo-hO6P_X1Wu_wU-fM2AHQEwnBqNs7rXdv7Znj5v9pv2Lp6Rg</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Zhao, Bo</creator><creator>Li, Guangping</creator><creator>Peng, Jianjun</creator><creator>Ren, Lihui</creator><creator>Lei, Licheng</creator><creator>Ye, Huiming</creator><creator>Wang, Zuoyan</creator><creator>Zhao, Sheng</creator><general>Springer US</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210201</creationdate><title>CircMACF1 Attenuates Acute Myocardial Infarction Through miR-500b-5p-EMP1 Axis</title><author>Zhao, Bo ; Li, Guangping ; Peng, Jianjun ; Ren, Lihui ; Lei, Licheng ; Ye, Huiming ; Wang, Zuoyan ; Zhao, Sheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-e42cd76aa14e5a5bfa21528488bb61b6d249d4c88ea061f551464720547f9983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedicine</topic><topic>Cardiology</topic><topic>Human Genetics</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original Article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Bo</creatorcontrib><creatorcontrib>Li, Guangping</creatorcontrib><creatorcontrib>Peng, Jianjun</creatorcontrib><creatorcontrib>Ren, Lihui</creatorcontrib><creatorcontrib>Lei, Licheng</creatorcontrib><creatorcontrib>Ye, Huiming</creatorcontrib><creatorcontrib>Wang, Zuoyan</creatorcontrib><creatorcontrib>Zhao, Sheng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cardiovascular translational research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Bo</au><au>Li, Guangping</au><au>Peng, Jianjun</au><au>Ren, Lihui</au><au>Lei, Licheng</au><au>Ye, Huiming</au><au>Wang, Zuoyan</au><au>Zhao, Sheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CircMACF1 Attenuates Acute Myocardial Infarction Through miR-500b-5p-EMP1 Axis</atitle><jtitle>Journal of cardiovascular translational research</jtitle><stitle>J. of Cardiovasc. Trans. Res</stitle><addtitle>J Cardiovasc Transl Res</addtitle><date>2021-02-01</date><risdate>2021</risdate><volume>14</volume><issue>1</issue><spage>161</spage><epage>172</epage><pages>161-172</pages><issn>1937-5387</issn><eissn>1937-5395</eissn><abstract>It is widely accepted that circular RNA (circRNA) plays an important role in cardiovascular diseases. Therefore, this experiment aimed to investigate the pathogenesis of circMACF1 in acute myocardial infarction (AMI). qRT-PCR and immunoblotting were used to detect the expression levels of circMACF1, miR-500b-5p, and epithelial membrane protein 1 (EMP1). The role of circMACF1, miR-500b-5p, and EMP1 in cardiomyocyte apoptosis was assessed using annexin V-FITC/PI. Echocardiographic assessment, serum creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH), myocardial infarct size, and TUNEL staining were applied in our research. In the MI group, the expression levels of circMACF1 and EMP1 were decreased with the increasing expression level of miR-500b-5p. CircMACF1 upregulated the expression of EMP1 as a sponge of miR-500b-5p, and circMACF1 was a direct target of miR-500b-5p. CircMACF1 impaired the progression of AMI by modulating the miR-500b-5p/EMP1 axis. CircMACF1 may be a potential therapeutic target for treating AMI.
Graphical Abstract
CircMACF1 upregulated EMP1 expression by sponge miR-500b-5p.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32162171</pmid><doi>10.1007/s12265-020-09976-5</doi><tpages>12</tpages></addata></record> |
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| subjects | Biomedical Engineering and Bioengineering Biomedicine Cardiology Human Genetics Medicine Medicine & Public Health Original Article |
| title | CircMACF1 Attenuates Acute Myocardial Infarction Through miR-500b-5p-EMP1 Axis |
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