Efficient CRISPR/Cas9 Gene Editing in Uncultured Naive Mouse T Cells for In Vivo Studies

CRISPR/Cas9 technologies have revolutionized our understanding of gene function in complex biological settings, including T cell immunology. Current CRISPR-mediated gene editing strategies in T cells require in vitro stimulation or culture that can both preclude the study of unmanipulated naive T ce...

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Veröffentlicht in:	The Journal of immunology (1950) 2020-04, Vol.204 (8), p.2308-2315
Hauptverfasser:	Nüssing, Simone, House, Imran G, Kearney, Conor J, Chen, Amanda X Y, Vervoort, Stephin J, Beavis, Paul A, Oliaro, Jane, Johnstone, Ricky W, Trapani, Joseph A, Parish, Ian A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals CD8-Positive T-Lymphocytes - immunology Clustered Regularly Interspaced Short Palindromic Repeats - genetics Clustered Regularly Interspaced Short Palindromic Repeats - immunology CRISPR-Cas Systems - genetics CRISPR-Cas Systems - immunology Electroporation Female Gene Editing Male Mice Mice, Inbred C57BL Mice, Knockout Polymorphism, Single Nucleotide - genetics Polymorphism, Single Nucleotide - immunology
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