5F peptide promotes endothelial differentiation of bone marrow stem cells through activation of ERK1/2 signaling
Synthetic apolipoprotein A-I (apoA-I) mimetic peptide 5F exhibits anti-atherosclerotic ability with largely unknown mechanism(s). Bone marrow (BM)-derived endothelial progenitor cells (EPCs) play a critical role in vascular integrity and function. The objective of the present study was to evaluate t...
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| Veröffentlicht in: | European journal of pharmacology 2020-06, Vol.876, p.173051-173051, Article 173051 |
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| container_title | European journal of pharmacology |
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| creator | Zhang, Jia Cui, Yuqi Li, Xin Xiao, Yuan Liu, Lingjuan Jia, Fengpeng He, Jianfeng Xie, Xiaoyun Parthasarathy, Sampath Hao, Hong Fang, Ningyuan |
| description | Synthetic apolipoprotein A-I (apoA-I) mimetic peptide 5F exhibits anti-atherosclerotic ability with largely unknown mechanism(s). Bone marrow (BM)-derived endothelial progenitor cells (EPCs) play a critical role in vascular integrity and function. The objective of the present study was to evaluate the effect of 5F on endothelial differentiation of BM stem cells and related mechanisms. Murine BM multipotent adult progenitor cells (MAPCs) were induced to differentiate into endothelial cells in vitro with or without 5F. The expression of endothelial markers vWF, Flk-1 and CD31 was significantly increased in the cells treated with 5F with enhanced in vitro vascular tube formation and LDL uptake without significant changes on proliferation and stem cell maker Oct-4 expression. Phosphorylated ERK1/2, not Akt, was significantly increased in 5F-treated cells. Treatment of MAPCs with PD98059 or small interfering RNA against ERK2 substantially attenuated ERK1/2 phosphorylation, and effectively prevented 5F-induced enhancement of endothelial differentiation of MAPCs. In vivo studies revealed that 5F increased EPCs number in the BM in mice after acute hindlimb ischemia that was effectively prevented with PD98059 treatment. These data supported the conclusion that 5F promoted endothelial differentiation of MAPCs through activation of ERK1/2 signaling. |
| doi_str_mv | 10.1016/j.ejphar.2020.173051 |
| format | Article |
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Bone marrow (BM)-derived endothelial progenitor cells (EPCs) play a critical role in vascular integrity and function. The objective of the present study was to evaluate the effect of 5F on endothelial differentiation of BM stem cells and related mechanisms. Murine BM multipotent adult progenitor cells (MAPCs) were induced to differentiate into endothelial cells in vitro with or without 5F. The expression of endothelial markers vWF, Flk-1 and CD31 was significantly increased in the cells treated with 5F with enhanced in vitro vascular tube formation and LDL uptake without significant changes on proliferation and stem cell maker Oct-4 expression. Phosphorylated ERK1/2, not Akt, was significantly increased in 5F-treated cells. Treatment of MAPCs with PD98059 or small interfering RNA against ERK2 substantially attenuated ERK1/2 phosphorylation, and effectively prevented 5F-induced enhancement of endothelial differentiation of MAPCs. In vivo studies revealed that 5F increased EPCs number in the BM in mice after acute hindlimb ischemia that was effectively prevented with PD98059 treatment. These data supported the conclusion that 5F promoted endothelial differentiation of MAPCs through activation of ERK1/2 signaling.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/j.ejphar.2020.173051</identifier><identifier>PMID: 32145325</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Apolipoprotein A-I mimetic peptide ; Bone marrow stem cell ; Bone Marrow Transplantation ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Endothelial differentiation ; Endothelial progenitor cell ; Endothelial Progenitor Cells - cytology ; Endothelial Progenitor Cells - drug effects ; Endothelial Progenitor Cells - metabolism ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; ERK1/2 ; Intercellular Signaling Peptides and Proteins - pharmacology ; MAP Kinase Signaling System - drug effects ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - drug effects ; Mesenchymal Stem Cells - metabolism ; Mice, Inbred C57BL ; Mitogen-Activated Protein Kinase 1 - genetics ; Octamer Transcription Factor-3 - genetics ; Rats ; RNA, Small Interfering - genetics ; Transfection</subject><ispartof>European journal of pharmacology, 2020-06, Vol.876, p.173051-173051, Article 173051</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-5046f7f381352d0f5a647d11cb8b1139a289c183c30beeaa50b94c78f34bb173</citedby><cites>FETCH-LOGICAL-c362t-5046f7f381352d0f5a647d11cb8b1139a289c183c30beeaa50b94c78f34bb173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014299920301436$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32145325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Jia</creatorcontrib><creatorcontrib>Cui, Yuqi</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Xiao, Yuan</creatorcontrib><creatorcontrib>Liu, Lingjuan</creatorcontrib><creatorcontrib>Jia, Fengpeng</creatorcontrib><creatorcontrib>He, Jianfeng</creatorcontrib><creatorcontrib>Xie, Xiaoyun</creatorcontrib><creatorcontrib>Parthasarathy, Sampath</creatorcontrib><creatorcontrib>Hao, Hong</creatorcontrib><creatorcontrib>Fang, Ningyuan</creatorcontrib><title>5F peptide promotes endothelial differentiation of bone marrow stem cells through activation of ERK1/2 signaling</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>Synthetic apolipoprotein A-I (apoA-I) mimetic peptide 5F exhibits anti-atherosclerotic ability with largely unknown mechanism(s). Bone marrow (BM)-derived endothelial progenitor cells (EPCs) play a critical role in vascular integrity and function. The objective of the present study was to evaluate the effect of 5F on endothelial differentiation of BM stem cells and related mechanisms. Murine BM multipotent adult progenitor cells (MAPCs) were induced to differentiate into endothelial cells in vitro with or without 5F. The expression of endothelial markers vWF, Flk-1 and CD31 was significantly increased in the cells treated with 5F with enhanced in vitro vascular tube formation and LDL uptake without significant changes on proliferation and stem cell maker Oct-4 expression. Phosphorylated ERK1/2, not Akt, was significantly increased in 5F-treated cells. Treatment of MAPCs with PD98059 or small interfering RNA against ERK2 substantially attenuated ERK1/2 phosphorylation, and effectively prevented 5F-induced enhancement of endothelial differentiation of MAPCs. In vivo studies revealed that 5F increased EPCs number in the BM in mice after acute hindlimb ischemia that was effectively prevented with PD98059 treatment. These data supported the conclusion that 5F promoted endothelial differentiation of MAPCs through activation of ERK1/2 signaling.</description><subject>Animals</subject><subject>Apolipoprotein A-I mimetic peptide</subject><subject>Bone marrow stem cell</subject><subject>Bone Marrow Transplantation</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Endothelial differentiation</subject><subject>Endothelial progenitor cell</subject><subject>Endothelial Progenitor Cells - cytology</subject><subject>Endothelial Progenitor Cells - drug effects</subject><subject>Endothelial Progenitor Cells - metabolism</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>ERK1/2</subject><subject>Intercellular Signaling Peptides and Proteins - pharmacology</subject><subject>MAP Kinase Signaling System - drug effects</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - drug effects</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Mice, Inbred C57BL</subject><subject>Mitogen-Activated Protein Kinase 1 - genetics</subject><subject>Octamer Transcription Factor-3 - genetics</subject><subject>Rats</subject><subject>RNA, Small Interfering - genetics</subject><subject>Transfection</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhoMoOo6-gUiWbjrm2stGEHFUHBDEfUjTk5kMbVOTjOLb26Hq0tWBw3cu_4fQBSULSmh-vV3AdtjosGCEja2CE0kP0IyWRZWRgrJDNCOEioxVVXWCTmPcEkJkxeQxOuGMCsmZnKFBLvEAQ3IN4CH4zieIGPrGpw20Tre4cdZCgD45nZzvsbe49j3gTofgP3FM0GEDbRtx2gS_W2-wNsl9_MH3r8_0muHo1r1uXb8-Q0dWtxHOf-ocvS3v3-4es9XLw9Pd7SozPGcpk0TktrC8pFyyhlipc1E0lJq6rCnllWZlZWjJDSc1gNaS1JUwRWm5qOtRxhxdTWvHUO87iEl1Lu7_1D34XVSMF4KLvCjzERUTaoKPMYBVQ3BjvC9FidqrVls1qVZ71WpSPY5d_lzY1R00f0O_bkfgZgJgjPnhIKhoHPQGGhfAJNV49_-Fb1imkcM</recordid><startdate>20200605</startdate><enddate>20200605</enddate><creator>Zhang, Jia</creator><creator>Cui, Yuqi</creator><creator>Li, Xin</creator><creator>Xiao, Yuan</creator><creator>Liu, Lingjuan</creator><creator>Jia, Fengpeng</creator><creator>He, Jianfeng</creator><creator>Xie, Xiaoyun</creator><creator>Parthasarathy, Sampath</creator><creator>Hao, Hong</creator><creator>Fang, Ningyuan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200605</creationdate><title>5F peptide promotes endothelial differentiation of bone marrow stem cells through activation of ERK1/2 signaling</title><author>Zhang, Jia ; Cui, Yuqi ; Li, Xin ; Xiao, Yuan ; Liu, Lingjuan ; Jia, Fengpeng ; He, Jianfeng ; Xie, Xiaoyun ; Parthasarathy, Sampath ; Hao, Hong ; Fang, Ningyuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-5046f7f381352d0f5a647d11cb8b1139a289c183c30beeaa50b94c78f34bb173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Apolipoprotein A-I mimetic peptide</topic><topic>Bone marrow stem cell</topic><topic>Bone Marrow Transplantation</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Endothelial differentiation</topic><topic>Endothelial progenitor cell</topic><topic>Endothelial Progenitor Cells - cytology</topic><topic>Endothelial Progenitor Cells - drug effects</topic><topic>Endothelial Progenitor Cells - metabolism</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>ERK1/2</topic><topic>Intercellular Signaling Peptides and Proteins - pharmacology</topic><topic>MAP Kinase Signaling System - drug effects</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - drug effects</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Mice, Inbred C57BL</topic><topic>Mitogen-Activated Protein Kinase 1 - genetics</topic><topic>Octamer Transcription Factor-3 - genetics</topic><topic>Rats</topic><topic>RNA, Small Interfering - genetics</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Jia</creatorcontrib><creatorcontrib>Cui, Yuqi</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Xiao, Yuan</creatorcontrib><creatorcontrib>Liu, Lingjuan</creatorcontrib><creatorcontrib>Jia, Fengpeng</creatorcontrib><creatorcontrib>He, Jianfeng</creatorcontrib><creatorcontrib>Xie, Xiaoyun</creatorcontrib><creatorcontrib>Parthasarathy, Sampath</creatorcontrib><creatorcontrib>Hao, Hong</creatorcontrib><creatorcontrib>Fang, Ningyuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Jia</au><au>Cui, Yuqi</au><au>Li, Xin</au><au>Xiao, Yuan</au><au>Liu, Lingjuan</au><au>Jia, Fengpeng</au><au>He, Jianfeng</au><au>Xie, Xiaoyun</au><au>Parthasarathy, Sampath</au><au>Hao, Hong</au><au>Fang, Ningyuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>5F peptide promotes endothelial differentiation of bone marrow stem cells through activation of ERK1/2 signaling</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>2020-06-05</date><risdate>2020</risdate><volume>876</volume><spage>173051</spage><epage>173051</epage><pages>173051-173051</pages><artnum>173051</artnum><issn>0014-2999</issn><eissn>1879-0712</eissn><abstract>Synthetic apolipoprotein A-I (apoA-I) mimetic peptide 5F exhibits anti-atherosclerotic ability with largely unknown mechanism(s). Bone marrow (BM)-derived endothelial progenitor cells (EPCs) play a critical role in vascular integrity and function. The objective of the present study was to evaluate the effect of 5F on endothelial differentiation of BM stem cells and related mechanisms. Murine BM multipotent adult progenitor cells (MAPCs) were induced to differentiate into endothelial cells in vitro with or without 5F. The expression of endothelial markers vWF, Flk-1 and CD31 was significantly increased in the cells treated with 5F with enhanced in vitro vascular tube formation and LDL uptake without significant changes on proliferation and stem cell maker Oct-4 expression. Phosphorylated ERK1/2, not Akt, was significantly increased in 5F-treated cells. Treatment of MAPCs with PD98059 or small interfering RNA against ERK2 substantially attenuated ERK1/2 phosphorylation, and effectively prevented 5F-induced enhancement of endothelial differentiation of MAPCs. In vivo studies revealed that 5F increased EPCs number in the BM in mice after acute hindlimb ischemia that was effectively prevented with PD98059 treatment. These data supported the conclusion that 5F promoted endothelial differentiation of MAPCs through activation of ERK1/2 signaling.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>32145325</pmid><doi>10.1016/j.ejphar.2020.173051</doi><tpages>1</tpages></addata></record> |
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| ispartof | European journal of pharmacology, 2020-06, Vol.876, p.173051-173051, Article 173051 |
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| subjects | Animals Apolipoprotein A-I mimetic peptide Bone marrow stem cell Bone Marrow Transplantation Cell Differentiation - drug effects Cell Proliferation - drug effects Endothelial differentiation Endothelial progenitor cell Endothelial Progenitor Cells - cytology Endothelial Progenitor Cells - drug effects Endothelial Progenitor Cells - metabolism Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism ERK1/2 Intercellular Signaling Peptides and Proteins - pharmacology MAP Kinase Signaling System - drug effects Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - drug effects Mesenchymal Stem Cells - metabolism Mice, Inbred C57BL Mitogen-Activated Protein Kinase 1 - genetics Octamer Transcription Factor-3 - genetics Rats RNA, Small Interfering - genetics Transfection |
| title | 5F peptide promotes endothelial differentiation of bone marrow stem cells through activation of ERK1/2 signaling |
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