Morphological, Molecular, and Pathological Appraisal of Hymenolepis nana (Hymenolepididae) Infecting Laboratory Mice ( Mus musculus )
Hymenolepis nana, typically a parasite found in conventionally established mouse colonies, has zoonotic potential characterized by autoinfection and direct life cycle. The objective of this study was to determine the rate of parasite infection in laboratory mice. The hymenolepidide cestode infected...
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| Veröffentlicht in: | Microscopy and microanalysis 2020-04, Vol.26 (2), p.348-362 |
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| creator | Al-Olayan, Ebtsam Elamin, Maha Alshehri, Eman Aloufi, Abeer Alanazi, Zainab Almayouf, Mina Bakr, Lamia Abdel-Gaber, Rewaida |
| description | Hymenolepis nana, typically a parasite found in conventionally established mouse colonies, has zoonotic potential characterized by autoinfection and direct life cycle. The objective of this study was to determine the rate of parasite infection in laboratory mice. The hymenolepidide cestode infected 40% of the 50 mice sampled. The rate of infection in males (52%) was higher than in females (28%). Morphological studies on the cestode parasite showed that worms had a globular scolex with four suckers, a retractable rostellum with 20-30 hooks, and a short unsegmented neck. In addition, the remaining strobila consisted of immature, mature, and gravid proglottids, irregularly alternating genital pores, lobulated ovaries, postovarian vitelline glands, and uteri with up to 200 eggs in their gravid proglottids. The parasite taxonomy was confirmed by using molecular characterization based on the sequence analysis of the mitochondrial cytochrome c oxidase subunit 1 (mtCOX1) gene. The parasite recovered was up to 80% identical to other species in GenBank. High blast scores and low divergence were noted between the isolated parasite and previously described H. nana (gb| AP017666.1). The phylogenetic analysis using the COX1 sequence places this hymenolepidid species of the order Cyclophyllidea. |
| doi_str_mv | 10.1017/S1431927620000161 |
| format | Article |
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The objective of this study was to determine the rate of parasite infection in laboratory mice. The hymenolepidide cestode infected 40% of the 50 mice sampled. The rate of infection in males (52%) was higher than in females (28%). Morphological studies on the cestode parasite showed that worms had a globular scolex with four suckers, a retractable rostellum with 20-30 hooks, and a short unsegmented neck. In addition, the remaining strobila consisted of immature, mature, and gravid proglottids, irregularly alternating genital pores, lobulated ovaries, postovarian vitelline glands, and uteri with up to 200 eggs in their gravid proglottids. The parasite taxonomy was confirmed by using molecular characterization based on the sequence analysis of the mitochondrial cytochrome c oxidase subunit 1 (mtCOX1) gene. The parasite recovered was up to 80% identical to other species in GenBank. High blast scores and low divergence were noted between the isolated parasite and previously described H. nana (gb| AP017666.1). The phylogenetic analysis using the COX1 sequence places this hymenolepidid species of the order Cyclophyllidea.</description><identifier>ISSN: 1431-9276</identifier><identifier>EISSN: 1435-8115</identifier><identifier>DOI: 10.1017/S1431927620000161</identifier><identifier>PMID: 32131927</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Alcohol ; Animals ; Cestoda ; Cyclooxygenase 1 - genetics ; Cytochrome-c oxidase ; Cytochromes ; Deoxyribonucleic acid ; Disease ; Disease Models, Animal ; Divergence ; DNA ; DNA, Helminth ; Eggs ; Female ; Gender differences ; Glands ; Hooks ; Hymenolepiasis - pathology ; Hymenolepis nana ; Hymenolepis nana - anatomy & histology ; Hymenolepis nana - genetics ; Infections ; Laboratory animals ; Life cycles ; Male ; Mice ; Mitochondria ; Morphology ; Ovaries ; Parasites ; Phylogenetics ; Phylogeny ; Rodentia ; Rodents ; Sequence analysis ; Software ; Taxonomy ; Worms ; Zoonoses</subject><ispartof>Microscopy and microanalysis, 2020-04, Vol.26 (2), p.348-362</ispartof><rights>Copyright © Microscopy Society of America 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c329t-75f4e47c42cea0b1ff3abdd35936fc652dce6d7e7c80a30bb8e01a314b33057f3</citedby><cites>FETCH-LOGICAL-c329t-75f4e47c42cea0b1ff3abdd35936fc652dce6d7e7c80a30bb8e01a314b33057f3</cites><orcidid>0000-0001-9263-6871</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32131927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Al-Olayan, Ebtsam</creatorcontrib><creatorcontrib>Elamin, Maha</creatorcontrib><creatorcontrib>Alshehri, Eman</creatorcontrib><creatorcontrib>Aloufi, Abeer</creatorcontrib><creatorcontrib>Alanazi, Zainab</creatorcontrib><creatorcontrib>Almayouf, Mina</creatorcontrib><creatorcontrib>Bakr, Lamia</creatorcontrib><creatorcontrib>Abdel-Gaber, Rewaida</creatorcontrib><title>Morphological, Molecular, and Pathological Appraisal of Hymenolepis nana (Hymenolepididae) Infecting Laboratory Mice ( Mus musculus )</title><title>Microscopy and microanalysis</title><addtitle>Microsc Microanal</addtitle><description>Hymenolepis nana, typically a parasite found in conventionally established mouse colonies, has zoonotic potential characterized by autoinfection and direct life cycle. The objective of this study was to determine the rate of parasite infection in laboratory mice. The hymenolepidide cestode infected 40% of the 50 mice sampled. The rate of infection in males (52%) was higher than in females (28%). Morphological studies on the cestode parasite showed that worms had a globular scolex with four suckers, a retractable rostellum with 20-30 hooks, and a short unsegmented neck. In addition, the remaining strobila consisted of immature, mature, and gravid proglottids, irregularly alternating genital pores, lobulated ovaries, postovarian vitelline glands, and uteri with up to 200 eggs in their gravid proglottids. The parasite taxonomy was confirmed by using molecular characterization based on the sequence analysis of the mitochondrial cytochrome c oxidase subunit 1 (mtCOX1) gene. The parasite recovered was up to 80% identical to other species in GenBank. High blast scores and low divergence were noted between the isolated parasite and previously described H. nana (gb| AP017666.1). The phylogenetic analysis using the COX1 sequence places this hymenolepidid species of the order Cyclophyllidea.</description><subject>Alcohol</subject><subject>Animals</subject><subject>Cestoda</subject><subject>Cyclooxygenase 1 - genetics</subject><subject>Cytochrome-c oxidase</subject><subject>Cytochromes</subject><subject>Deoxyribonucleic acid</subject><subject>Disease</subject><subject>Disease Models, Animal</subject><subject>Divergence</subject><subject>DNA</subject><subject>DNA, Helminth</subject><subject>Eggs</subject><subject>Female</subject><subject>Gender differences</subject><subject>Glands</subject><subject>Hooks</subject><subject>Hymenolepiasis - pathology</subject><subject>Hymenolepis nana</subject><subject>Hymenolepis nana - anatomy & histology</subject><subject>Hymenolepis nana - genetics</subject><subject>Infections</subject><subject>Laboratory animals</subject><subject>Life cycles</subject><subject>Male</subject><subject>Mice</subject><subject>Mitochondria</subject><subject>Morphology</subject><subject>Ovaries</subject><subject>Parasites</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Rodentia</subject><subject>Rodents</subject><subject>Sequence analysis</subject><subject>Software</subject><subject>Taxonomy</subject><subject>Worms</subject><subject>Zoonoses</subject><issn>1431-9276</issn><issn>1435-8115</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkd1KxDAQhYMo_qw-gDcS8GYXtppp2qZ7KaKusIuCel2mabJW2qYm7cU-gO9t9scVNDc5zHxnZuAQcg7sChiI6xeIOExCkYTMP0hgjxz7UhykAPH-WkOw6h-RE-c-PMOZSA7JEQ9hbTwmX3Nj23dTmUUpsRrTuamU7Cu0Y4pNQZ-x2zXpTdtaLJ1XRtPpslaNh9vS0QYbpMPfSlEWqEb0sdFKdmWzoDPMjcXO2CWdl1LRIZ33jta986u8GJ2SA42VU2fbf0De7u9eb6fB7Onh8fZmFkgeTrpAxDpSkZBRKBWyHLTmmBcFjyc80TKJw0KqpBBKyJQhZ3meKgbIIco5Z7HQfECGm7mtNZ-9cl1Wl06qqsJGmd5lIReQxjGwyKOXf9AP09vGX-epSQJpmCbMU7ChpDXOWaWz1pY12mUGLFtllP3LyHsutpP7vFbFzvETCv8GmySMZQ</recordid><startdate>202004</startdate><enddate>202004</enddate><creator>Al-Olayan, Ebtsam</creator><creator>Elamin, Maha</creator><creator>Alshehri, Eman</creator><creator>Aloufi, Abeer</creator><creator>Alanazi, Zainab</creator><creator>Almayouf, Mina</creator><creator>Bakr, Lamia</creator><creator>Abdel-Gaber, Rewaida</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9263-6871</orcidid></search><sort><creationdate>202004</creationdate><title>Morphological, Molecular, and Pathological Appraisal of Hymenolepis nana (Hymenolepididae) Infecting Laboratory Mice ( Mus musculus )</title><author>Al-Olayan, Ebtsam ; Elamin, Maha ; Alshehri, Eman ; Aloufi, Abeer ; Alanazi, Zainab ; Almayouf, Mina ; Bakr, Lamia ; Abdel-Gaber, Rewaida</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-75f4e47c42cea0b1ff3abdd35936fc652dce6d7e7c80a30bb8e01a314b33057f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alcohol</topic><topic>Animals</topic><topic>Cestoda</topic><topic>Cyclooxygenase 1 - 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Academic</collection><jtitle>Microscopy and microanalysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Al-Olayan, Ebtsam</au><au>Elamin, Maha</au><au>Alshehri, Eman</au><au>Aloufi, Abeer</au><au>Alanazi, Zainab</au><au>Almayouf, Mina</au><au>Bakr, Lamia</au><au>Abdel-Gaber, Rewaida</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological, Molecular, and Pathological Appraisal of Hymenolepis nana (Hymenolepididae) Infecting Laboratory Mice ( Mus musculus )</atitle><jtitle>Microscopy and microanalysis</jtitle><addtitle>Microsc Microanal</addtitle><date>2020-04</date><risdate>2020</risdate><volume>26</volume><issue>2</issue><spage>348</spage><epage>362</epage><pages>348-362</pages><issn>1431-9276</issn><eissn>1435-8115</eissn><abstract>Hymenolepis nana, typically a parasite found in conventionally established mouse colonies, has zoonotic potential characterized by autoinfection and direct life cycle. The objective of this study was to determine the rate of parasite infection in laboratory mice. The hymenolepidide cestode infected 40% of the 50 mice sampled. The rate of infection in males (52%) was higher than in females (28%). Morphological studies on the cestode parasite showed that worms had a globular scolex with four suckers, a retractable rostellum with 20-30 hooks, and a short unsegmented neck. In addition, the remaining strobila consisted of immature, mature, and gravid proglottids, irregularly alternating genital pores, lobulated ovaries, postovarian vitelline glands, and uteri with up to 200 eggs in their gravid proglottids. The parasite taxonomy was confirmed by using molecular characterization based on the sequence analysis of the mitochondrial cytochrome c oxidase subunit 1 (mtCOX1) gene. The parasite recovered was up to 80% identical to other species in GenBank. High blast scores and low divergence were noted between the isolated parasite and previously described H. nana (gb| AP017666.1). The phylogenetic analysis using the COX1 sequence places this hymenolepidid species of the order Cyclophyllidea.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>32131927</pmid><doi>10.1017/S1431927620000161</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-9263-6871</orcidid></addata></record> |
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| ispartof | Microscopy and microanalysis, 2020-04, Vol.26 (2), p.348-362 |
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| source | MEDLINE; Cambridge Journals |
| subjects | Alcohol Animals Cestoda Cyclooxygenase 1 - genetics Cytochrome-c oxidase Cytochromes Deoxyribonucleic acid Disease Disease Models, Animal Divergence DNA DNA, Helminth Eggs Female Gender differences Glands Hooks Hymenolepiasis - pathology Hymenolepis nana Hymenolepis nana - anatomy & histology Hymenolepis nana - genetics Infections Laboratory animals Life cycles Male Mice Mitochondria Morphology Ovaries Parasites Phylogenetics Phylogeny Rodentia Rodents Sequence analysis Software Taxonomy Worms Zoonoses |
| title | Morphological, Molecular, and Pathological Appraisal of Hymenolepis nana (Hymenolepididae) Infecting Laboratory Mice ( Mus musculus ) |
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