Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens
The present study explored the mechanism of Zn-methionine (Zn-Met) influencing eggshell quality of laying hens and investigated whether the mechanism was related to Ca deposition. Hyline grey layers (n 384, 38 weeks old) were divided into four groups: 0 mg Zn/kg, 40, 80 mg Zn/kg as Zn-Met, and 80 mg...
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| Veröffentlicht in: | British journal of nutrition 2019-11, Vol.122 (9), p.961-973 |
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| creator | Li, Lanlan Miao, Liping Zhu, Mingkun Wang, Liansong Zou, Xiaoting |
| description | The present study explored the mechanism of Zn-methionine (Zn-Met) influencing eggshell quality of laying hens and investigated whether the mechanism was related to Ca deposition. Hyline grey layers (n 384, 38 weeks old) were divided into four groups: 0 mg Zn/kg, 40, 80 mg Zn/kg as Zn-Met, and 80 mg Zn/kg as zinc sulphate (ZnSO4). Eggshell quality, Zn contents, Zn-containing enzyme activities and expressions of shell matrix proteins in eggshell gland (ESG) were analysed. Zn-Met treatment at 80 mg/kg increased (P < 0·05) egg weight and eggshell strength throughout the experiments. The 80 mg/kg Zn-Met group (P < 0·05) had decreased mammillary knob width and larger relative atomic weight percentage of Ca, stronger signal intensity of Ca in linear distribution and the Ca was more evenly distributed in the transversal surface of eggshell. Zn contents (P < 0·001) in yolk and serum, Ca, albumin (Alb) levels in ESG as well as carbonic anhydrase (CA) activity in serum (P < 0·05) and mRNA levels of CA and Ca-binding protein-d28k (CaBP-D28k) (P < 0·001) in the 80 mg/kg Zn-Met group were the highest among all treatments. In conclusion, shell strength as one of eggshell qualities was mostly related to mammillary cone width in ultrastructure caused by the pattern of Ca deposition in eggshell formation. Also, the increase in Zn-Met-induced Ca deposition may be due to the increased Zn contents in serum and tissues, which were attributable to the increased CA concentrations in serum, Ca, Alb levels and up-regulated CA and CaBP-D28k mRNA levels in ESG. |
| doi_str_mv | 10.1017/S000711451900206X |
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Hyline grey layers (n 384, 38 weeks old) were divided into four groups: 0 mg Zn/kg, 40, 80 mg Zn/kg as Zn-Met, and 80 mg Zn/kg as zinc sulphate (ZnSO4). Eggshell quality, Zn contents, Zn-containing enzyme activities and expressions of shell matrix proteins in eggshell gland (ESG) were analysed. Zn-Met treatment at 80 mg/kg increased (P < 0·05) egg weight and eggshell strength throughout the experiments. The 80 mg/kg Zn-Met group (P < 0·05) had decreased mammillary knob width and larger relative atomic weight percentage of Ca, stronger signal intensity of Ca in linear distribution and the Ca was more evenly distributed in the transversal surface of eggshell. Zn contents (P < 0·001) in yolk and serum, Ca, albumin (Alb) levels in ESG as well as carbonic anhydrase (CA) activity in serum (P < 0·05) and mRNA levels of CA and Ca-binding protein-d28k (CaBP-D28k) (P < 0·001) in the 80 mg/kg Zn-Met group were the highest among all treatments. In conclusion, shell strength as one of eggshell qualities was mostly related to mammillary cone width in ultrastructure caused by the pattern of Ca deposition in eggshell formation. Also, the increase in Zn-Met-induced Ca deposition may be due to the increased Zn contents in serum and tissues, which were attributable to the increased CA concentrations in serum, Ca, Alb levels and up-regulated CA and CaBP-D28k mRNA levels in ESG.</description><identifier>ISSN: 0007-1145</identifier><identifier>EISSN: 1475-2662</identifier><identifier>DOI: 10.1017/S000711451900206X</identifier><identifier>PMID: 32124714</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Albumins ; Atomic properties ; Calcium ; Calcium-binding protein ; Carbonic anhydrase ; Carbonic anhydrases ; Deposition ; Diet ; Egg shells ; Eggs ; Enzymatic activity ; Gene expression ; Metabolism and Metabolic Studies ; Methionine ; Mineralization ; mRNA ; Nutrition research ; Physiology ; Protein synthesis ; Proteins ; Quality ; Scanning electron microscopy ; Ultrastructure ; Weight ; Yolk ; Zinc ; Zinc sulfate</subject><ispartof>British journal of nutrition, 2019-11, Vol.122 (9), p.961-973</ispartof><rights>The Authors 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-e294bcd9a9937b7c0ae2fc97698a532b6d910c1772d4303ae34693b8d042f6f3</citedby><orcidid>0000-0002-0728-2333</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S000711451900206X/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32124714$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Lanlan</creatorcontrib><creatorcontrib>Miao, Liping</creatorcontrib><creatorcontrib>Zhu, Mingkun</creatorcontrib><creatorcontrib>Wang, Liansong</creatorcontrib><creatorcontrib>Zou, Xiaoting</creatorcontrib><title>Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens</title><title>British journal of nutrition</title><addtitle>Br J Nutr</addtitle><description>The present study explored the mechanism of Zn-methionine (Zn-Met) influencing eggshell quality of laying hens and investigated whether the mechanism was related to Ca deposition. Hyline grey layers (n 384, 38 weeks old) were divided into four groups: 0 mg Zn/kg, 40, 80 mg Zn/kg as Zn-Met, and 80 mg Zn/kg as zinc sulphate (ZnSO4). Eggshell quality, Zn contents, Zn-containing enzyme activities and expressions of shell matrix proteins in eggshell gland (ESG) were analysed. Zn-Met treatment at 80 mg/kg increased (P < 0·05) egg weight and eggshell strength throughout the experiments. The 80 mg/kg Zn-Met group (P < 0·05) had decreased mammillary knob width and larger relative atomic weight percentage of Ca, stronger signal intensity of Ca in linear distribution and the Ca was more evenly distributed in the transversal surface of eggshell. Zn contents (P < 0·001) in yolk and serum, Ca, albumin (Alb) levels in ESG as well as carbonic anhydrase (CA) activity in serum (P < 0·05) and mRNA levels of CA and Ca-binding protein-d28k (CaBP-D28k) (P < 0·001) in the 80 mg/kg Zn-Met group were the highest among all treatments. In conclusion, shell strength as one of eggshell qualities was mostly related to mammillary cone width in ultrastructure caused by the pattern of Ca deposition in eggshell formation. Also, the increase in Zn-Met-induced Ca deposition may be due to the increased Zn contents in serum and tissues, which were attributable to the increased CA concentrations in serum, Ca, Alb levels and up-regulated CA and CaBP-D28k mRNA levels in ESG.</description><subject>Albumins</subject><subject>Atomic properties</subject><subject>Calcium</subject><subject>Calcium-binding protein</subject><subject>Carbonic anhydrase</subject><subject>Carbonic anhydrases</subject><subject>Deposition</subject><subject>Diet</subject><subject>Egg shells</subject><subject>Eggs</subject><subject>Enzymatic activity</subject><subject>Gene expression</subject><subject>Metabolism and Metabolic Studies</subject><subject>Methionine</subject><subject>Mineralization</subject><subject>mRNA</subject><subject>Nutrition research</subject><subject>Physiology</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Quality</subject><subject>Scanning electron microscopy</subject><subject>Ultrastructure</subject><subject>Weight</subject><subject>Yolk</subject><subject>Zinc</subject><subject>Zinc sulfate</subject><issn>0007-1145</issn><issn>1475-2662</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kU1LxDAQhoMouq7-AC8S8OKlmo82aY7iNwge9OCtpMlkN9Kma9Me1rM_3Cy7KiiehmGe951hXoSOKDmjhMrzJ0KIpDQvqCKEEfGyhSY0l0XGhGDbaLIaZ6v5HtqP8TW1JSVqF-1xRlkuaT5BH1ceBt0vsbbWD74LuHP43QeTtTDMU-8DYB9cM0IwYDHMZnEOTYPfRt34YYnrJHUOzODDDBvdGD-22MKii2s7H340rutb_bWk0cuVZA4hHqAdp5sIh5s6Rc8318-Xd9nD4-395cVDZrjkQwZM5bWxSivFZS0N0cCcUVKoUhec1cIqSgyVktmcE66B50LxurQkZ044PkWna9tF372NEIeq9dGkw3SAbowV45IUTJWiTOjJL_S1G_uQjksULQUraCETRdeU6bsYe3DVovdt-mZFSbVKqPqTUNIcb5zHugX7rfiKJAF8Y6rbuvd2Bj-7_7f9BNAOnF4</recordid><startdate>20191114</startdate><enddate>20191114</enddate><creator>Li, Lanlan</creator><creator>Miao, Liping</creator><creator>Zhu, Mingkun</creator><creator>Wang, Liansong</creator><creator>Zou, Xiaoting</creator><general>Cambridge University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7T5</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0728-2333</orcidid></search><sort><creationdate>20191114</creationdate><title>Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens</title><author>Li, Lanlan ; Miao, Liping ; Zhu, Mingkun ; Wang, Liansong ; Zou, Xiaoting</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-e294bcd9a9937b7c0ae2fc97698a532b6d910c1772d4303ae34693b8d042f6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Albumins</topic><topic>Atomic properties</topic><topic>Calcium</topic><topic>Calcium-binding protein</topic><topic>Carbonic anhydrase</topic><topic>Carbonic anhydrases</topic><topic>Deposition</topic><topic>Diet</topic><topic>Egg shells</topic><topic>Eggs</topic><topic>Enzymatic activity</topic><topic>Gene expression</topic><topic>Metabolism and Metabolic Studies</topic><topic>Methionine</topic><topic>Mineralization</topic><topic>mRNA</topic><topic>Nutrition research</topic><topic>Physiology</topic><topic>Protein synthesis</topic><topic>Proteins</topic><topic>Quality</topic><topic>Scanning electron microscopy</topic><topic>Ultrastructure</topic><topic>Weight</topic><topic>Yolk</topic><topic>Zinc</topic><topic>Zinc sulfate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Lanlan</creatorcontrib><creatorcontrib>Miao, Liping</creatorcontrib><creatorcontrib>Zhu, Mingkun</creatorcontrib><creatorcontrib>Wang, Liansong</creatorcontrib><creatorcontrib>Zou, Xiaoting</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Lanlan</au><au>Miao, Liping</au><au>Zhu, Mingkun</au><au>Wang, Liansong</au><au>Zou, Xiaoting</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens</atitle><jtitle>British journal of nutrition</jtitle><addtitle>Br J Nutr</addtitle><date>2019-11-14</date><risdate>2019</risdate><volume>122</volume><issue>9</issue><spage>961</spage><epage>973</epage><pages>961-973</pages><issn>0007-1145</issn><eissn>1475-2662</eissn><abstract>The present study explored the mechanism of Zn-methionine (Zn-Met) influencing eggshell quality of laying hens and investigated whether the mechanism was related to Ca deposition. Hyline grey layers (n 384, 38 weeks old) were divided into four groups: 0 mg Zn/kg, 40, 80 mg Zn/kg as Zn-Met, and 80 mg Zn/kg as zinc sulphate (ZnSO4). Eggshell quality, Zn contents, Zn-containing enzyme activities and expressions of shell matrix proteins in eggshell gland (ESG) were analysed. Zn-Met treatment at 80 mg/kg increased (P < 0·05) egg weight and eggshell strength throughout the experiments. The 80 mg/kg Zn-Met group (P < 0·05) had decreased mammillary knob width and larger relative atomic weight percentage of Ca, stronger signal intensity of Ca in linear distribution and the Ca was more evenly distributed in the transversal surface of eggshell. Zn contents (P < 0·001) in yolk and serum, Ca, albumin (Alb) levels in ESG as well as carbonic anhydrase (CA) activity in serum (P < 0·05) and mRNA levels of CA and Ca-binding protein-d28k (CaBP-D28k) (P < 0·001) in the 80 mg/kg Zn-Met group were the highest among all treatments. In conclusion, shell strength as one of eggshell qualities was mostly related to mammillary cone width in ultrastructure caused by the pattern of Ca deposition in eggshell formation. Also, the increase in Zn-Met-induced Ca deposition may be due to the increased Zn contents in serum and tissues, which were attributable to the increased CA concentrations in serum, Ca, Alb levels and up-regulated CA and CaBP-D28k mRNA levels in ESG.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>32124714</pmid><doi>10.1017/S000711451900206X</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-0728-2333</orcidid></addata></record> |
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| subjects | Albumins Atomic properties Calcium Calcium-binding protein Carbonic anhydrase Carbonic anhydrases Deposition Diet Egg shells Eggs Enzymatic activity Gene expression Metabolism and Metabolic Studies Methionine Mineralization mRNA Nutrition research Physiology Protein synthesis Proteins Quality Scanning electron microscopy Ultrastructure Weight Yolk Zinc Zinc sulfate |
| title | Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens |
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